In a previous study, we uncovered the anticonvulsant properties of turmeric oil and its sesquiterpenoids (ar-turmerone, α-, β-turmerone and α-atlantone) in both zebrafish and mouse models of chemically-induced seizures using pentylenetetrazole (PTZ). In this follow-up study, we aimed at evaluating the anticonvulsant activity of ar-turmerone further. A more in-depth anticonvulsant evaluation of ar-turmerone was therefore carried out in the i.v. PTZ and 6-Hz mouse models. The potential toxic effects of ar-turmerone were evaluated using the beam walking test to assess mouse motor function and balance. In addition, determination of the concentration-time profile of ar-turmerone was carried out for a more extended evaluation of its bioavailability in the mouse brain. Ar-turmerone displayed anticonvulsant properties in both acute seizure models in mice and modulated the expression patterns of two seizure-related genes (c-fos and brain-derived neurotrophic factor [bdnf]) in zebrafish. Importantly, no effects on motor function and balance were observed in mice after treatment with ar-turmerone even after administering a dose 500-fold higher than the effective dose in the 6-Hz model. In addition, quantification of its concentration in mouse brains revealed rapid absorption after i.p. administration, capacity to cross the BBB and long-term brain residence. Hence, our results provide additional information on the anticonvulsant properties of ar-turmerone and support further evaluation towards elucidating its mechanism of action, bioavailability, toxicity and potential clinical application.

The study investigates the phytochemical profile, antioxidant capacity, and antimicrobial activities of ethanol (ChL-EtOH) and ethyl acetate (ChL-EtOAc) extracts from Chamaenerion latifolium L. (ChL) harvested in Kazakhstan. The ChL-EtOH extract exhibited higher total phenolic (267.48 ± 3.44 mg GAE/g DE) and flavonoid content (24.18 ± 1.06 mg QE/g DE) compared to ChL-EtOAc. HPLC-UV-ESI/MS identified key phenolic acids and flavonoids, including gallic acid, chlorogenic acid, and quercetin 3-glucoside. FT-IR analysis confirmed the presence of characteristic functional groups. Antioxidant assays revealed strong DPPH scavenging and FRAP activities, with ChL-EtOH showing superior results (IC₅₀ = 21.31 ± 0.65 μg/mL and 18.13 ± 0.15 μg/mL, respectively). Additionally, ChL-EtOH displayed notable antimicrobial efficacy against Gram-positive and Gram-negative bacteria, as well as the fungal strain Candida albicans. These findings suggest that ethanol extraction is more efficient for isolating bioactive compounds from ChL, underscoring its potential for pharmaceutical and nutraceutical applications.

The accumulated waste generated from industries severely affects environmental conditions. Using waste as a construction material or soil stabilization is an emerging area in the construction industry. Introducing new additive materials to strengthen local soils using industrial waste is an inexpensive and more effective method to improve the soil. In light of this, this study aims to develop environmentally clean construction materials for stabilizing natural loam (NL) using red mud (RM), blast furnace slag (BFS), and lime production waste (LPW). Nine different mixtures were prepared with four different combinations of RM (20, 30, and 40%), BFS (25, 30 and 35%), LPW (4, 6 and 8%), and various content of NL. X-ray diffraction (XRD), X-ray fluorescence (XRF), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), atomic absorption spectroscopy (AAS), and axial compressive strength were examined. The results indicated that the optimum strength was obtained from the sample containing 40% of RM, 35% of BFS, and 8% of LPW. The observed compressive strength of the sample for 90 days was 7.38 MPa, water resistance was 7.12 MPa, and frost resistance was 7.35 MP, with low linear expansion meeting the demands for first class construction materials of the Kazakh norms. The mineral composition analysis evidenced the lack of heavy metals contaminants and hazardous compounds. Based on strength and environmental performance, RM, BFS, LPW, and NL mix can be used as a road base material. This process is believed to reduce environmental pollution related to RM and BFS, and lower the road base cost.

According to the Bureau of National Statistics of the Republic of Kazakhstan, by the end of 2023, approximately 120 million tons of municipal solid waste (MSW) had been generated across over 3200 landfills in the country. About 4.5 million tons are generated annually, of which only about 15% are recycled. The accumulation of both unsorted and sorted waste poses significant environmental risks, primarily through the generation of methane, a greenhouse gas that is 28 times more dangerous than carbon dioxide in contributing to the planet’s greenhouse effect over a century and 84 times more effective over a 20-year timeframe. The objective of this research is to examine the physicochemical composition, as well as the physical and thermal-chemical properties, of municipal solid waste from six cities in Kazakhstan: Astana, Almaty, Shymkent, Aktobe, Karaganda, and Ust-Kamenogorsk. Unlike existing studies, this study has a uniform waste sample, which includes the complete emptying of dozens of containers from different areas of the cities under consideration. Thus, the average composition of solid waste across the cities was maintained. Analysis of the physicochemical composition was conducted for both unsorted and sorted municipal solid waste from all cities, determining the total and analytical moisture content, ash content, and volatile matter, as well as the higher and lower calorific values. The calorific value of unsorted waste by city was as follows, in kJ/kg: Astana,8850.37; Almaty, 9244.57; Atobe, 9596.41; Shymkent, 9425.48; Karaganda, 8902.8; Ust-Kamenogorsk, 9669.07. The calorific value of sorted waste was as follows, in kJ/kg: Astana, 11,922.79; Almaty, 11,692.31; Atobe, 11,913.13; Shymkent, 12,494.38; Karaganda, 11,671.92; Ust-Kamenogorsk, 12,462.52. The efficiency of sorting was estimated as the first stage of MSW processing. The efficiency factor of the manual sorting process in practice was 0.4–0.8. The results obtained enable the evaluation of technologies for the effective management of municipal solid waste and facilitate experimental investigations into semi-industrial pyrolysis, combustion, plasma processing, and composting facilities.