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Helicobacter pylori is a Gram-negative bacterium that colonizes the stomach of about 60% of people worldwide. The search for new drugs with activity against H. pylori is now a hotspot in the effective and safe control of this bacterium. Therefore, the aim of this research was to determine the antibacterial activity of extracts from selected plants of the Papaveraceae family against planktonic and biofilm forms of the multidrug-resistant clinical strain of H. pylori using a broad spectrum of analytical in vitro methods. It was revealed that among the tested extracts, those obtained from Corydalis cheilanthifolia and Chelidonium majus were the most active, with minimal inhibitory concentrations (MICs) of 64 µg/mL and 128 µg/mL, respectively. High concentrations of both extracts showed cytotoxicity against cell lines of human hepatic origin. Therefore, we attempted to lower their MICs through the use of a synergistic combination with synthetic antimicrobials as well as by applying cellulose as a drug carrier. Using checkerboard assays, we determined that both extracts presented synergistic interactions with amoxicillin (AMX) and 3-bromopyruvate (3-BP) (FICI = 0.5) and additive relationships with sertraline (SER) (FICI = 0.75). The antibiofilm activity of extracts and their combinations with AMX, 3-BP, or SER, was analyzed by two methods, i.e., the microcapillary overgrowth under flow conditions (the Bioflux system) and assessment of the viability of lawn biofilms after exposure to drugs released from bacterial cellulose (BC) carriers. Using both methods, we observed a several-fold decrease in the level of H. pylori biofilm, indicating the ability of the tested compounds to eradicate the microbial biofilm. The obtained results indicate that application of plant-derived extracts from the Papaveraceae family combined with synthetic antimicrobials, absorbed into organic BC carrier, may be considered a promising way of fighting biofilm-forming H. pylori.
Paweł Krzyżek; Adam Junka; Wojciech Słupski; Arleta Dołowacka-Jóźwiak; Bartosz J. Płachno; Aleksandra Sobiecka; Adam Matkowski; Grzegorz Chodaczek; Tadeusz Płusa; Grażyna Gościniak; Sylwia Zielińska. Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori. Pathogens 2021, 10, 1033 .
AMA StylePaweł Krzyżek, Adam Junka, Wojciech Słupski, Arleta Dołowacka-Jóźwiak, Bartosz J. Płachno, Aleksandra Sobiecka, Adam Matkowski, Grzegorz Chodaczek, Tadeusz Płusa, Grażyna Gościniak, Sylwia Zielińska. Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori. Pathogens. 2021; 10 (8):1033.
Chicago/Turabian StylePaweł Krzyżek; Adam Junka; Wojciech Słupski; Arleta Dołowacka-Jóźwiak; Bartosz J. Płachno; Aleksandra Sobiecka; Adam Matkowski; Grzegorz Chodaczek; Tadeusz Płusa; Grażyna Gościniak; Sylwia Zielińska. 2021. "Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori." Pathogens 10, no. 8: 1033.
The antimicrobial properties of herbs from Papaveraceae have been used in medicine for centuries. Nevertheless, mutual relationships between the individual bioactive substances contained in these plants remain poorly elucidated. In this work, phytochemical composition of extracts from the aerial and underground parts of five Papaveraceae species (Chelidonium majus L., Corydalis cava (L.) Schweigg. and Körte, C. cheilanthifolia Hemsl., C. pumila (Host) Rchb., and Fumaria vaillantii Loisel.) were examined using LC-ESI-MS/MS with a triple quadrupole analyzer. Large differences in the quality and quantity of all analyzed compounds were observed between species of different genera and also within one genus. Two groups of metabolites predominated in the phytochemical profiles. These were isoquinoline alkaloids and, in smaller amounts, non-phenolic carboxylic acids and phenolic compounds. In aerial and underground parts, 22 and 20 compounds were detected, respectively. These included: seven isoquinoline alkaloids: protopine, allocryptopine, coptisine, berberine, chelidonine, sanguinarine, and chelerythrine; five of their derivatives as well as non-alkaloids: malic acid, trans-aconitic acid, quinic acid, salicylic acid, trans-caffeic acid, p-coumaric acid, chlorogenic acid, quercetin, and kaempferol; and vanillin. The aerial parts were much richer in phenolic compounds regardless of the plant species. Characterized extracts were studied for their antimicrobial potential against planktonic and biofilm-producing cells of S. aureus, P. aeruginosa, and C. albicans. The impact of the extracts on cellular metabolic activity and biofilm biomass production was evaluated. Moreover, the antimicrobial activity of the extracts introduced to the polymeric carrier made of bacterial cellulose was assessed. Extracts of C. cheilanthifolia were found to be the most effective against all tested human pathogens. Multiple regression tests indicated a high antimicrobial impact of quercetin in extracts of aerial parts against planktonic cells of S. aureus, P. aeruginosa, and C. albicans, and no direct correlation between the composition of other bioactive substances and the results of antimicrobial activity were found. Conclusively, further investigations are required to identify the relations between recognized and unrecognized compounds within extracts and their biological properties.
Sylwia Zielińska; Magdalena Dziągwa-Becker; Adam Junka; Ewelina Piątczak; Anna Jezierska-Domaradzka; Malwina Brożyna; Justyna Paleczny; Aleksandra Sobiecka; Wojciech Słupski; Eleonora Mess; Mariusz Kucharski; Serhat Çiçek; Christian Zidorn; Adam Matkowski. Screening Papaveraceae as Novel Antibiofilm Natural-Based Agents. Molecules 2021, 26, 4778 .
AMA StyleSylwia Zielińska, Magdalena Dziągwa-Becker, Adam Junka, Ewelina Piątczak, Anna Jezierska-Domaradzka, Malwina Brożyna, Justyna Paleczny, Aleksandra Sobiecka, Wojciech Słupski, Eleonora Mess, Mariusz Kucharski, Serhat Çiçek, Christian Zidorn, Adam Matkowski. Screening Papaveraceae as Novel Antibiofilm Natural-Based Agents. Molecules. 2021; 26 (16):4778.
Chicago/Turabian StyleSylwia Zielińska; Magdalena Dziągwa-Becker; Adam Junka; Ewelina Piątczak; Anna Jezierska-Domaradzka; Malwina Brożyna; Justyna Paleczny; Aleksandra Sobiecka; Wojciech Słupski; Eleonora Mess; Mariusz Kucharski; Serhat Çiçek; Christian Zidorn; Adam Matkowski. 2021. "Screening Papaveraceae as Novel Antibiofilm Natural-Based Agents." Molecules 26, no. 16: 4778.
Corydalis and Pseudofumaria are two closely related genera from the Papaveraceae subfamily Fumarioideae with Corydalis solida (C. solida) and Pseudofumaria lutea (P. lutea) as two representative species. Phytochemical analysis revealed significant differences in the quality and quantity of isoquinoline alkaloids, phenolic compounds and non-phenolic carboxylic acids between aerial and underground parts of both species. Using the Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) technique, 21 compounds were identified: five protoberberine derivatives, three protopine derivatives, four phenanthridine derivatives, as well as three carboxylic acids, two hydroxycinnamic acids, one chlorogenic acid, one phenolic aldehyde, and two flavonoids. Moroever, significant differences in the content of individual compounds were observed between the two studied species. The phytochemical profile of C. solida showed a higher variety of compounds that were present in lower amounts, whereas P. lutea extracts contained fewer compounds but in larger quantities. Protopine was one of the most abundant constituents in C. solida (440–1125 µg/g d.w.) and in P. lutea (1036–1934 µg/g d.w.). Moreover, considerable amounts of coptisine (1526 µg/g) and quercetin (3247 µg/g) were detected in the aerial parts of P. lutea. Extracts from aerial and underground parts of both species were also examined for the antimicrobial potential against S. aureus, P. aeruginosa and C. albicans. P. lutea herb extract was the most effective (MIC at 0.39 mg/L) against all three pathogens.
Sylwia Zielińska; Magdalena Dziągwa-Becker; Ewelina Piątczak; Anna Jezierska-Domaradzka; Malwina Brożyna; Adam Junka; Mariusz Kucharski; Serhat Sezai ÇİÇek; Christian Zidorn; Adam Matkowski. Phytochemical Composition and Antimicrobial Activity of Corydalis solida and Pseudofumaria lutea. Molecules 2020, 25, 3591 .
AMA StyleSylwia Zielińska, Magdalena Dziągwa-Becker, Ewelina Piątczak, Anna Jezierska-Domaradzka, Malwina Brożyna, Adam Junka, Mariusz Kucharski, Serhat Sezai ÇİÇek, Christian Zidorn, Adam Matkowski. Phytochemical Composition and Antimicrobial Activity of Corydalis solida and Pseudofumaria lutea. Molecules. 2020; 25 (16):3591.
Chicago/Turabian StyleSylwia Zielińska; Magdalena Dziągwa-Becker; Ewelina Piątczak; Anna Jezierska-Domaradzka; Malwina Brożyna; Adam Junka; Mariusz Kucharski; Serhat Sezai ÇİÇek; Christian Zidorn; Adam Matkowski. 2020. "Phytochemical Composition and Antimicrobial Activity of Corydalis solida and Pseudofumaria lutea." Molecules 25, no. 16: 3591.
Moluccella laevis L. (Lamiaceae) is an annual ornamental plant, naturally occurring in the areas of Western and Central Asia (Irano-Turanian area). It is a popular ornamental species, but its phytochemical profile remains poorly studied. This study presents the first report on in vitro cultures of M. laevis. The influence of two types of illumination and plant growth regulators (PGRs) supplementation on the morphogenetic response and the content of phenolic compounds were examined. Shoots were cultured on Murashige and Skoog (MS) solid basal medium. White light or photosynthetic active radiation (PAR) as well as three concentrations of 6-benzylaminopurine (BA) 2, 3 or 4 µM and one of the three auxins: 1-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA) or 4-amino-3,5,6-trichloropicolinic acid (picloram—PIC) at concentration of 0.5 µM were used for shoot proliferation. The highest number of axillary shoots per explant (3.4) was obtained for shoots grown on MS medium supplemented with 3-µM BA and 0.5-μM NAA under PAR light. However, the effect of PGRs treatment on shoot growth and development was stronger than the influence of light. The phytochemical analysis using Ultra-Performance Liquid Chromatography–Mass Spectrometry allowed identification of four hydroxycinnamic acids (caffeic, ferulic, rosmarinic, chlorogenic) and two cyanidin glycosides (cyanidin 3-O-galactoside, and cyanidin 3-O-malonylglucoside) in shoot clusters and callus tissue.
Sylwia Zielińska; Ewelina Piątczak; Weronika Kozłowska; Aleksandra Bohater; Anna Jezierska-Domaradzka; Joanna Kolniak-Ostek; Adam Matkowski. LED illumination and plant growth regulators’ effects on growth and phenolic acids accumulation in Moluccella laevis L. in vitro cultures. Acta Physiologiae Plantarum 2020, 42, 1 -10.
AMA StyleSylwia Zielińska, Ewelina Piątczak, Weronika Kozłowska, Aleksandra Bohater, Anna Jezierska-Domaradzka, Joanna Kolniak-Ostek, Adam Matkowski. LED illumination and plant growth regulators’ effects on growth and phenolic acids accumulation in Moluccella laevis L. in vitro cultures. Acta Physiologiae Plantarum. 2020; 42 (5):1-10.
Chicago/Turabian StyleSylwia Zielińska; Ewelina Piątczak; Weronika Kozłowska; Aleksandra Bohater; Anna Jezierska-Domaradzka; Joanna Kolniak-Ostek; Adam Matkowski. 2020. "LED illumination and plant growth regulators’ effects on growth and phenolic acids accumulation in Moluccella laevis L. in vitro cultures." Acta Physiologiae Plantarum 42, no. 5: 1-10.
Due to certain differences in terms of molecular structure, isoquinoline alkaloids from Chelidonium majus engage in various biological activities. Apart from their well-documented antimicrobial potential, some phenanthridine and protoberberine derivatives as well as C. majus extract present with anti-inflammatory and cytotoxic effects. In this study, the LC–MS/MS method was used to determine alkaloids, phenolic acids, carboxylic acids, and hydroxybenzoic acids. We investigated five individually tested alkaloids (coptisine, berberine, chelidonine, chelerythrine, and sanguinarine) as well as C. majus root extract for their effect on the secretion of IL-1β, IL-8, and TNF-α in human polymorphonuclear leukocytes (neutrophils). Berberine, chelidonine, and chelerythrine significantly decreased the secretion of TNF-α in a concentration-dependent manner. Sanguinarine was found to be the most potent inhibitor of IL-1β secretion. However, the overproduction of IL-8 and TNF-α and a high cytotoxicity for these compounds were observed. Coptisine was highly cytotoxic and slightly decreased the secretion of the studied cytokines. The extract (1.25–12.5 μg/mL) increased cytokine secretion in a concentration-dependent manner, but an increase in cytotoxicity was also noted. The alkaloids were active at very low concentrations (0.625–2.5 μM), but their potential cytotoxic effects, except for chelidonine and chelerythrine, should not be ignored.
Sylwia Zielińska; Monika Ewa Czerwińska; Magdalena Dziągwa-Becker; Andrzej Dryś; Mariusz Kucharski; Anna Jezierska-Domaradzka; Bartosz J. Płachno; Adam Matkowski. Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils. Molecules 2020, 25, 842 .
AMA StyleSylwia Zielińska, Monika Ewa Czerwińska, Magdalena Dziągwa-Becker, Andrzej Dryś, Mariusz Kucharski, Anna Jezierska-Domaradzka, Bartosz J. Płachno, Adam Matkowski. Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils. Molecules. 2020; 25 (4):842.
Chicago/Turabian StyleSylwia Zielińska; Monika Ewa Czerwińska; Magdalena Dziągwa-Becker; Andrzej Dryś; Mariusz Kucharski; Anna Jezierska-Domaradzka; Bartosz J. Płachno; Adam Matkowski. 2020. "Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils." Molecules 25, no. 4: 842.
Agastache rugosa (Fisch. & C.A.Mey.) Kuntze is an East Asian medicinal and aromatic crop rich in bioactive polyphenols, but their content is highly variable. To get insight into mechanisms regulating the biosynthesis of phenolics, leaves of one-, two- and three-year-old A. rugosa and their mother plants, were analyzed for phytochemical profile and key biosynthetic genes expression. Using UHPLC-QTOF-MS thirty-six polyphenolic compounds were found, out of which twelve were quantified. The major compounds (rosmarinic acid, apigenin glucoside, chlorogenic acid) content differed significantly between plants of different age and different age of their mother plant as confirmed by multivariate statistics. There was also a close relationship between one-year old plant groups and their respective plant progenitors which suggests an existence of a transgenerational phenomenon. Quantitative real-time PCR showed that transcript levels of: phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate: CoA ligase (4CL), tyrosine aminotransferase (TAT), hydroxyphenylpyruvate reductase (HPPR), chalcone synthase (CHS) and chalcone isomerase (CHI) did not reflect end-product concentrations. Only expression of rosmarinic acid synthase (ArRAS) correlated with its end-product accumulation. We propose that ArRAS is the rate-limiting step for RA biosynthesis in A. rugosa.
Monika Bielecka; Sylwia Zielińska; Bartosz Pencakowski; Marta Stafiniak; Sylwester Ślusarczyk; Anna Prescha; Adam Matkowski. Age-related variation of polyphenol content and expression of phenylpropanoid biosynthetic genes in Agastache rugosa. Industrial Crops and Products 2019, 141, 111743 .
AMA StyleMonika Bielecka, Sylwia Zielińska, Bartosz Pencakowski, Marta Stafiniak, Sylwester Ślusarczyk, Anna Prescha, Adam Matkowski. Age-related variation of polyphenol content and expression of phenylpropanoid biosynthetic genes in Agastache rugosa. Industrial Crops and Products. 2019; 141 ():111743.
Chicago/Turabian StyleMonika Bielecka; Sylwia Zielińska; Bartosz Pencakowski; Marta Stafiniak; Sylwester Ślusarczyk; Anna Prescha; Adam Matkowski. 2019. "Age-related variation of polyphenol content and expression of phenylpropanoid biosynthetic genes in Agastache rugosa." Industrial Crops and Products 141, no. : 111743.
Chelidonium majus (Papaveraceae) extracts exhibit antimicrobial activity due to the complex alkaloid composition. The aim of the research was to evaluate the antimicrobial potential of extracts from wild plants and in vitro cultures, as well as seven major individual alkaloids. Plant material derived from different natural habitats and in vitro cultures was used for the phytochemical analysis and antimicrobial tests. The composition of alkaloids was analyzed using chromatographic techniques (HPLC with DAD detection). The results have shown that roots contained higher number and amounts of alkaloids in comparison to aerial parts. All tested plant extracts manifested antimicrobial activity, related to different chemical structures of the alkaloids. Root extract used at 31.25–62.5 mg/L strongly reduced bacterial biomass. From the seven individually tested alkaloids, chelerythrine was the most effective against P. aeruginosa (MIC at 1.9 mg/L), while sanguinarine against S. aureus (MIC at 1.9 mg/L). Strong antifungal activity was observed against C. albicans when chelerythrine, chelidonine, and aerial parts extract were used. The experiments with plant extracts, individually tested alkaloids, and variable combinations of the latter allowed for a deeper insight into the potential mechanisms affecting the activity of this group of compounds.
Sylwia Zielińska; Magdalena Wójciak-Kosior; Magdalena Dziągwa-Becker; Michał Gleńsk; Ireneusz Sowa; Karol Fijałkowski; Danuta Rurańska-Smutnicka; Adam Matkowski; Adam Junka. The Activity of Isoquinoline Alkaloids and Extracts from Chelidonium majus against Pathogenic Bacteria and Candida sp. Toxins 2019, 11, 406 .
AMA StyleSylwia Zielińska, Magdalena Wójciak-Kosior, Magdalena Dziągwa-Becker, Michał Gleńsk, Ireneusz Sowa, Karol Fijałkowski, Danuta Rurańska-Smutnicka, Adam Matkowski, Adam Junka. The Activity of Isoquinoline Alkaloids and Extracts from Chelidonium majus against Pathogenic Bacteria and Candida sp. Toxins. 2019; 11 (7):406.
Chicago/Turabian StyleSylwia Zielińska; Magdalena Wójciak-Kosior; Magdalena Dziągwa-Becker; Michał Gleńsk; Ireneusz Sowa; Karol Fijałkowski; Danuta Rurańska-Smutnicka; Adam Matkowski; Adam Junka. 2019. "The Activity of Isoquinoline Alkaloids and Extracts from Chelidonium majus against Pathogenic Bacteria and Candida sp." Toxins 11, no. 7: 406.
Agastache rugosa (Fischer & C.A.Meyer) O.Kuntze (Lamiaceae) is an East Asian medicinal and aromatic plant. It is rich in polyphenolic compounds such as rosmarinic, chlorogenic, ferulic acids and apigenin glycosides. in vitro shoot cultures were used to study influence of various factors on polyphenol profile using Ultra Performance Liquid Chromatography coupled to high resolution mass spectrometry (UPLC-ESI-qTOF-MS). Large differences in the morphology and polyphenol profile were observed in experiments with various illumination (white fluorescent lamps or white and photosynthetically active radiation LEDs) and supplementation with plant growth regulators and amino acids. Shoots were cultured on the MS basal agar medium with or without plant growth regulators (6-benzylaminopurine - BA, indole-3-acetic acid – IAA), or supplemented with different concentrations of phenylpropanoid biosynthesis precursor - l-phenylalanine or an amino acid mixture (casein hydrolysate). The composition of polyphenols in methanolic extracts was analyzed using UPLC-DAD-qTOF-MS. Three phenolic acids: cryptochlorogenic acid, feruloyl-quinic acid, rosmarinic acid, a rosmarinic acid methyl ester and two isomeric ferulic acid glucosides, as well as one flavonoid – an apigenin derivative were detected. Rosmarinic acid (RA) was the most abundant compound found in the analyzed plant material. Supplementation with amino acids resulted in highest content of RA in shoots cultured for 196 days on media containing either low concentration (1 mg/L) of l-phenylalanine or two of the highest - 20, 50 mg/L. The effect of casein hydrolysate supplementation was noticed from the beginning of shoot culture and reached maximum of 23.3 mg/g on 140 day. On the other hand, shoots that were growing under different illumination produced over 20 mg/g dw of RA after 70 days of culture. In conclusion, the production of phenolic compounds in A. rugosa in vitro shoots was influenced by the age of the shoot cultures, illumination regime and amino acids supplementation.
Sylwia Zielińska; Andrzej Dryś; Ewelina Piątczak; Joanna Kolniak-Ostek; Marta Podgórska; Jan Oszmiański; Adam Matkowski. Effect of LED illumination and amino acid supplementation on phenolic compounds profile in Agastache rugosa in vitro cultures. Phytochemistry Letters 2019, 31, 12 -19.
AMA StyleSylwia Zielińska, Andrzej Dryś, Ewelina Piątczak, Joanna Kolniak-Ostek, Marta Podgórska, Jan Oszmiański, Adam Matkowski. Effect of LED illumination and amino acid supplementation on phenolic compounds profile in Agastache rugosa in vitro cultures. Phytochemistry Letters. 2019; 31 ():12-19.
Chicago/Turabian StyleSylwia Zielińska; Andrzej Dryś; Ewelina Piątczak; Joanna Kolniak-Ostek; Marta Podgórska; Jan Oszmiański; Adam Matkowski. 2019. "Effect of LED illumination and amino acid supplementation on phenolic compounds profile in Agastache rugosa in vitro cultures." Phytochemistry Letters 31, no. : 12-19.
Chelidonium majus L. is a medicinal plant containing several alkaloids, such as chelidonine, chelerythrine, sanguinarine, protopine, and coptisine that are stored and secreted from laticifers—highly specialized elongated cells of aerial and terrestrial parts of the plant. For use as a drug, several cultivars of this specialized crop are cultivated but the content and composition of bioactive substances is variable. Based on the observation of a characteristic orange-yellow color of latex in dedifferentiated tissue of in vitro callus cultures we investigated the alkaloid composition and the anatomy of callus and organ cultures of C. majus maintained on media enriched in plant growth regulators and illuminated with white or photosynthetically active light. Alkaloid composition was analyzed using DAD-HPLC, whereas tissue and organ anatomy was studied using light and scanning electron microscopy. The fully developed organs and rhizogenic callus compared to the undifferentiated cells, produced larger proportion of alkaloids, and complex alkaloid composition. In roots, sanguinarine was the predominant alkaloid exceeding 4 mg/g dry mass. In microshoots, coptisine was the major compound, with the maximum content exceeding 14 mg/g, depending on the medium composition. In callus cultures, either protopine or sanguinarine predominated on different media and under different illumination, with maximum content over 13 mg/g dry mass of protopine reached under PAR (Photosynthetically Active Radiation), and sanguinarine over 0.9 mg/g. The presence of laticifers was confirmed with microscopy and the scanning electron microscopy revealed an abundant extracellular matrix in the callus tissues. These results suggest the high biosynthetic potential of in vitro grown organs and dedifferentiated callus tissue that are able to produce significant amounts of pharmacologically relevant alkaloids from C. majus in various proportions that depend on the culture conditions such as supplementation with growth substances and sugars as well as on the illumination with light of different spectrum.
Sylwia Zielinska; Magdalena Wójciak-Kosior; Bartosz J. Płachno; Ireneusz Sowa; Maciej Włodarczyk; Adam Matkowski. Quaternary alkaloids in Chelidonium majus in vitro cultures. Industrial Crops and Products 2018, 123, 17 -24.
AMA StyleSylwia Zielinska, Magdalena Wójciak-Kosior, Bartosz J. Płachno, Ireneusz Sowa, Maciej Włodarczyk, Adam Matkowski. Quaternary alkaloids in Chelidonium majus in vitro cultures. Industrial Crops and Products. 2018; 123 ():17-24.
Chicago/Turabian StyleSylwia Zielinska; Magdalena Wójciak-Kosior; Bartosz J. Płachno; Ireneusz Sowa; Maciej Włodarczyk; Adam Matkowski. 2018. "Quaternary alkaloids in Chelidonium majus in vitro cultures." Industrial Crops and Products 123, no. : 17-24.
The root of Symphytum officinale L. is commonly used in folk medicine to promote the wound healing, reduce the inflammation and in the treatment of broken bones. The objective of our investigation was to analyse the extract from S. officinale in term of its antioxidant activity and the effect on cell viability and proliferation of human skin fibroblast (HSF). Moreover, the quantification of main phenolics and allantoin was conducted using HPLC–DAD method. Five compounds were found: rosmarinic, p-hydroxybenzoic, caffeic, chlorogenic and p-coumaric acid. DPPH, FRAP and TPC assay showed the high antioxidant activity of the extract. MTT test proved the stimulatory effect on cell metabolism and viability of HSF cells. Moreover, no changes in cytoskeleton structure and cells shape were observed. The obtained results indicate that non-toxic extract from S. officinale root has strong antioxidant potential and a beneficial effect on human skin fibroblasts.
Ireneusz Sowa; Roman Paduch; Maciej Strzemski; Sylwia Zielińska; Ewelina Rydzik-Strzemska; Jan Sawicki; Ryszard Kocjan; Janusz Polkowski; Adam Matkowski; Michał Latalski; Magdalena Wójciak-Kosior. Proliferative and antioxidant activity of Symphytum officinale root extract. Natural Product Research 2017, 32, 605 -609.
AMA StyleIreneusz Sowa, Roman Paduch, Maciej Strzemski, Sylwia Zielińska, Ewelina Rydzik-Strzemska, Jan Sawicki, Ryszard Kocjan, Janusz Polkowski, Adam Matkowski, Michał Latalski, Magdalena Wójciak-Kosior. Proliferative and antioxidant activity of Symphytum officinale root extract. Natural Product Research. 2017; 32 (5):605-609.
Chicago/Turabian StyleIreneusz Sowa; Roman Paduch; Maciej Strzemski; Sylwia Zielińska; Ewelina Rydzik-Strzemska; Jan Sawicki; Ryszard Kocjan; Janusz Polkowski; Adam Matkowski; Michał Latalski; Magdalena Wójciak-Kosior. 2017. "Proliferative and antioxidant activity of Symphytum officinale root extract." Natural Product Research 32, no. 5: 605-609.
Sylwia Zielińska; Mariola Dąbrowska; Weronika Kozłowska; Danuta Kalemba; Renata Abel; Andrzej Dryś; Antoni Szumny; Adam Matkowski. Ontogenetic and trans-generational variation of essential oil composition in Agastache rugosa. Industrial Crops and Products 2017, 97, 612 -619.
AMA StyleSylwia Zielińska, Mariola Dąbrowska, Weronika Kozłowska, Danuta Kalemba, Renata Abel, Andrzej Dryś, Antoni Szumny, Adam Matkowski. Ontogenetic and trans-generational variation of essential oil composition in Agastache rugosa. Industrial Crops and Products. 2017; 97 ():612-619.
Chicago/Turabian StyleSylwia Zielińska; Mariola Dąbrowska; Weronika Kozłowska; Danuta Kalemba; Renata Abel; Andrzej Dryś; Antoni Szumny; Adam Matkowski. 2017. "Ontogenetic and trans-generational variation of essential oil composition in Agastache rugosa." Industrial Crops and Products 97, no. : 612-619.
Sylwia Zielińska; Joanna Kolniak-Ostek; Mariusz Dziadas; Jan Oszmiański; Adam Matkowski. Characterization of polyphenols in Agastache rugosa leaves and inflorescences by UPLC–qTOF–MS following FCPC separation. Journal of Liquid Chromatography & Related Technologies 2016, 39, 209 -219.
AMA StyleSylwia Zielińska, Joanna Kolniak-Ostek, Mariusz Dziadas, Jan Oszmiański, Adam Matkowski. Characterization of polyphenols in Agastache rugosa leaves and inflorescences by UPLC–qTOF–MS following FCPC separation. Journal of Liquid Chromatography & Related Technologies. 2016; 39 (4):209-219.
Chicago/Turabian StyleSylwia Zielińska; Joanna Kolniak-Ostek; Mariusz Dziadas; Jan Oszmiański; Adam Matkowski. 2016. "Characterization of polyphenols in Agastache rugosa leaves and inflorescences by UPLC–qTOF–MS following FCPC separation." Journal of Liquid Chromatography & Related Technologies 39, no. 4: 209-219.
The composition of volatile organic compounds emitted by in vitro shoots of Agastache rugosa (Fischer & C.A. Meyer) O. Kuntze (Lamiaceae) was studied using headspace solid-phase microextraction–gas chromatography–mass spectrometry and compared to the those emitted by adult plants and in vitro-germinated seedlings. Shoot-tip explants were cultured on a solid MS medium supplemented with either 4.4 μM 6-benzyladenine (BA), 9.3 μM kinetin, or 0.45 μM thidiazuron and with either 0.57 μM indole-3-acetic acid (IAA) or 0.41 μM picloram. Shoot proliferation was observed in all these treatments. The presence of these plant growth regulators in the culture medium significantly influenced the composition of volatiles as well as morphogenetic responses observed. The number and quality of regenerating shoots and frequency of axillary bud break were highest in medium containing the BA + IAA combination. Sixty-five compounds were identified in the headspace of the in vitro-produced material and plants cultivated in the field. The in vitro shoots emitted both hydrocarbon (limonene, α-pinene) and oxidized (menthone, isomenthone, pulegone) monoterpenes. The composition of monoterpenes differed depending on the type of auxin—rather than cytokinin—in the medium. The emission of phenylallyl compounds, such as estragole, a major compound in field-grown plants, was markedly lower in shoot cultures.
Sylwia Zielińska; Ewelina Piątczak; Danuta Kalemba; Adam Matkowski. Influence of plant growth regulators on volatiles produced by in vitro grown shoots of Agastache rugosa (Fischer & C.A.Meyer) O. Kuntze. Plant Cell, Tissue and Organ Culture (PCTOC) 2011, 107, 161 -167.
AMA StyleSylwia Zielińska, Ewelina Piątczak, Danuta Kalemba, Adam Matkowski. Influence of plant growth regulators on volatiles produced by in vitro grown shoots of Agastache rugosa (Fischer & C.A.Meyer) O. Kuntze. Plant Cell, Tissue and Organ Culture (PCTOC). 2011; 107 (1):161-167.
Chicago/Turabian StyleSylwia Zielińska; Ewelina Piątczak; Danuta Kalemba; Adam Matkowski. 2011. "Influence of plant growth regulators on volatiles produced by in vitro grown shoots of Agastache rugosa (Fischer & C.A.Meyer) O. Kuntze." Plant Cell, Tissue and Organ Culture (PCTOC) 107, no. 1: 161-167.