Despite the threat posed by tuberculosis (TB) to the protected European bison (Bison bonasus), no validated TB tests exist for this species. This pilot study evaluates two tests based on detecting cellular immunity for this purpose: interferon gamma release assay (IGRA) and tuberculin skin test (TST). Ten animals were subjected to ante-mortem and post-mortem examinations. IGRA was performed using a commercial test, and the comparative TST was performed in the eyelids. The lesions were assessed post-mortem and material was collected for mycobacterial culture. The isolated strains were subjected to genotyping. At post-mortem examination, five out of ten individuals demonstrated both tuberculous lesions and positive culture results (Mycobacterium caprae). Compared to the palpebral TST, the findings of the IGRA are easier to interpret when diagnosing tuberculosis in European bison.

Mycobacterial infections are significant issues in zoo animals, influencing animal welfare, conservation efforts, and the zoonotic potential of pathogens. Although tuberculosis is recognised to be highly dangerous, paratuberculosis can also lead to animal losses and is potentially dangerous for humans. The aim of the current study was to confirm whether Mycobacterium avium spp. paratuberculosis (MAP) infections are currently present in zoos in Poland. Faeces samples (n = 131) were collected from different animal species from eight zoos in Poland. The faeces were decontaminated and inoculated into Herrold’s Egg Yolk Media. The species was determined using commercial DNA testing. The IS900 was checked using RT-PCR. The culture was positive in seven samples: five with M. avium, one with Mycobacterium fortiatum, and one without any identified Mycobacterium species. RT-PCR confirmed MAP genetic material in nine animals. Our findings represent the first confirmation of MAP in bongo (Tragelaphus eurycerus), indicating that it is present in Polish zoological gardens. Fortunately, the disease can be monitored more easily due to recent legislation (the Animal Health Law).

In Poland, bovine tuberculosis (BTB) is most often detected in cattle, although some cases have also been reported in pigs and in other non-domestic species. BTB is believed to be mainly present in the Bieszczady region, in the south-eastern part of the country. The present study analyses the practical applications of the law regarding the eradication of tuberculosis among European bison in Poland, as well as the influence of legal rules in species conservation effects. Its aim is to present and evaluate the effectiveness of legal provisions in selected cases of BTB detected in European bison. The analysis highlights key “critical points” in the management of cases of BTB in European bison, which resulted from the problematic interpretation and application of the existing legal provisions. Findings indicate that before the Animal Health Law, the eradication of BTB in European bison in Poland was not regulated clearly enough by the applicable laws in force prior to 2021. This posed a threat to an effective reintroduction strategy for bison based on creating larger metapopulations and maintaining small, isolated reservoir herds (breading centers) for fallback in the case of infectious disease. It should be emphasized that in the current legal system, there is no doubt that a District Veterinary Officer has the competence to prevent and control tuberculosis in European bison.

In the period 1996–2012, two outbreaks of animal tuberculosis were noted in the population of free-living European bison (Bison bonasus caucasicus) in the Bieszczady Mountains, Southern Poland. As the European bison is an endangered species and particularly susceptible to tuberculosis, not to mention a national icon, the decision was made to test all deceased bison for TB in Poland. The screened bison were obtained by elimination due to poor health or natural death. A total of 159 European bison have been examined over the last 10 years. The individuals came from four regions of Poland (Białowieża Forest, Bieszczady Mountains, Borecka Forest, Knyszyńska Forest), not only from the area where tuberculosis is still endemic. Mycobacterium bovis and Mycobacterium avium spp. hominisuis were identified in two different herds. The isolation of M. bovis from European bison was the first case described in Poland. So far, the only causative agent of tuberculosis identified in European bison in Poland, both in the wild and in captive herds, was Mycobacterium caprae. The isolated M. bovis spoligotype has not previously been registered in international spoligotype databases so far. The obtained results highlight the need to monitor TB in European bison in Poland.

Introduction and objective: Bovine tuberculosis (BTB) remains an emerging problem in animals, and particular care is required in zoos to protect the health of the public visitors (zoonosis) and the unique animals kept in them, which may be endangered species. In larger zoos, the problem is...