A total of 636 1-day-old male Cobb chicks were randomly assigned to seven treatments. The chicks were offered feed and water ad libitum throughout the experimental period. The first three groups included different stocking densities of broiler birds (low stocking density, LSD: 23 kg/m²; medium stocking density, MSD: 34 kg birds/m²; and high stocking density HSD: 39 kg birds/m²). The LSD group was considered a control group. The other four groups included MSD or HSD broiler birds supplemented with either Vit E (100 mg/kg DM diet; MSDVE and HSDVE) or Zn (100 mg/kg DM diet; MSDZn and HSDZn) in their basal diet. The main findings indicated that HSD and MSD negatively affected (p < 0.05) all variables under investigation compared with LSD. Compared with LSD, broiler birds in the MSD and HSD groups had lower body weights and higher feed conversion ratios, higher concentrations of blood plasma hormones (triiodothyronine thyroxine and corticosterone), and downregulated expression levels of hepatic growth hormone and insulin-like growth factor-l. In addition, broiler birds stocked at medium or high densities resulted in less economic return and profit. Vit E or Zn supplementation to broiler birds stocked at medium or high densities significantly reversed all adverse effects of HSD (> 23 kg/m²) on growth performance, hormones, and gene expression. It could be recommended that adding Zn at a level of 100 mg/kg per DM diet allows increasing the stocking density of broiler birds from 23 kg/m² to 34 birds/m² while maintaining the birds, welfare and economic profit.

Background and Aim: Despite many trials, buffalo embryos have poor cryosurvivability because of their high lipid content. L-carnitine was found to be a lipid-reducing agent when added to oocyte and embryo culture media. The study aimed to determine the most effective concentration of L-carnitine to improve the oocyte developmental competence and cryotolerance of buffalo embryos. Materials and Methods: In vitro maturation and embryo culture media were supplemented with four concentrations of L-carnitine: 0 (control), 0.25, 0.5, and 1 mM. Good-quality embryos on 7 days were vitrified using mixtures of dimethyl sulfoxide and ethylene glycol at two concentrations (3.5 and 7 M). Results: The result showed that the cleavage and morula rates were significantly (p<0.05) higher in the 0.5 mM group. Blastocyst rates were significantly (p<0.05) higher at both 0.5 and 1 mM. The rates of viable embryos directly after thawing were significantly (p<0.05) increased in the 0.5 mM group. No significant difference was found in embryos cultured for 24 h after warming among all the groups. Conclusion: The addition of L-carnitine at a concentration of 0.5 mM to the culture media improves the oocyte developmental competence and cryotolerance of buffalo embryos directly after warming but not after 24 h of culture. Nevertheless, further studies must identify how L-carnitine exerts its beneficial micromechanisms.

Background and Aim: Artemisia annua (AA), used as a growth promoter in poultry, lowers feed costs and enhances economic efficiency. This study aimed to assess the impact of varying AA concentrations on broiler chicken growth, gene expression, and profitability. Materials and Methods: Two hundred 1-day-old male Cobb chicks were randomly allocated into four treatment groups, each containing five replicates and 10 birds. The experimental groups consisted of G1 (basal diet), G2 (basal diet with 0.3% AA), G3 (basal diet with 0.6% AA), and G4 (basal diet with 0.9% AA). The birds had continuous access to feed and water throughout the study. The experiment lasted for 42 days. we measured the growth performance (Feed intake, Life weight), carcass traits (weight after slaughter, dressed carcass, heart, gizzard, spleen, giblet and thymus weight), liver and spleen antioxidants (CAT, GSH, SOD), and gene expression of anti-inflammatory and immune- related genes. Results: The primary findings revealed that the addition of 0.6% AA had a positive impact (p < 0.05) on all investigated variables compared with the control and other groups. Dietary supplementation with 0.6% AA led to increased breast, giblet, skeleton, and total yield, and net return compared with the control group. Supplementation with AA exhibited antioxidant, anti-inflammatory, and immunological effects through improved levels of antioxidant superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in tissue homogenates of the liver and spleen. It also upregulated the relative messenger RNA levels of anti-inflammatory interleukin (IL)-10, SOD, CAT, and GSH-Px, whereas IL-1β and tumor necrosis factor-alpha were downregulated. Conclusion: The study found that AA is a promising replacement for antibiotics in poultry farming as a growth promoter for chickens. 0.6% AA in the broiler diet yielded the best results, striking a balance between superior performance and robust economic benefits. Keywords: Artemisia annua, broiler, carcass traits, economics, immunity, mRNA gene expression.

Studying testicular genes´ expression may give key insights into precise regulation of its functions that influence epididymal sperm quality. The current study aimed to investigate the abundance of candidate genes involved in the regulation of testicular functions specially those regulate sperm function (PLA2G4D, SPP1 and CLUAP1), testicular steroidogenic function (ESR1 and AR), materials transport (AQP12B and LCN15) and defense mechanisms (DEFB110, GPX5, SOCS3 and IL6). Therefore, blood samples and testes with epididymis were collected from mature middle-aged (5–10 years) dromedary camels (n = 45) directly prior and after their slaughtering, respectively, during breeding season. Sera were evaluated for testosterone level and testicular biometry was measured with caliper. The epididymal tail semen was evaluated manually. Samples were distinguished based on testosterone level, testicular biometry, as well as epididymal semen features into high and low fertile groups. Total RNA was isolated from testicular tissues and genes expression was done using qRT-PCR. Results revealed that testosterone levels were significantly (P < 0.005) higher in camels with good semen quality than those of low quality. There was a significant (P < 0.0001) increase in testicular weight, length, width, thickness, and volume in high fertile than low fertile camels. PLA2G4D, SPP1, CLUAP1, ESR1, AR, AQP12B, LCN15, DEFB110, GPX5 and SOCS3 genes were up-regulated (P < 0.001), and IL6 gene was down-regulated (P < 0.01) in the testes of high fertile camels compared to the low fertile one. Thus, it could be concluded that examined genes might be valuable monitors of testicular functional status and fertility in dromedary camels.

After the copulation process, some sperm start the long journey with an ultimate goal of fertilizing the oocyte. Inside the oviduct, sperm are stored, waiting for the ovulated oocyte where they bind to the apical surface of the oviduct cells, which in turn hold sperm to form a sperm nest. The essential functions of the sperm reservoir include attaching spermatozoa to oviduct epithelial cells, selecting intact, good-quality sperm with an end result of extending sperm life expectancy. The current study aimed to evaluate the fertilization ability of sperm that bind to cell aggregates from different parts of the oviduct (infundibulum-ampulla-isthmus), and to assess the effect of heparin and or progesterone (P4) on the in-vitro fertilization ability of sperm co-incubated with cell aggregates from the isthmus. In-vitro fertilization was identified as a cleaved oocyte to two cells or more. The sperm bound to cell aggregates from the isthmus improved the rate of in-vitro fertilization (48.09%) compared to aggregates from the infundibulum (36.90%) and ampulla (37.61%). Moreover, pre-treatment of mature COCs with heparin (40 μg/mL) and P4 (80 nanomolar) play a coactive role that improves the in-vitro fertilization ability of sperm bound to cell aggregates from isthmus (63.33%), compared to 42.61% in the absence of cells aggregates. In conclusion, binding to cell aggregates from isthmus improves the in-vitro fertilization ability of Bovine sperm. Additionally, heparin, together with P4, exerts a synergistic action that improves the in-vitro fertilizing potential of sperm attached to cell aggregates from the isthmus of the bovine oviduct.

Acaricide usage has led to the growing problem of resistance in ticks. A heavy tick burden and the presence of ticks on animals throughout the year, despite the monthly application of acaricides, in farms in the United Arab Emirates formed the motivation for this study. The objectives of this research were as follows: (a) to assess the acaricide resistance status of the most prevalent tick Hyalomma spp. to widely used acaricides Cypermethrin and Deltamethrin; (b) to identify the association of farm management practices and farm-level risk factors with the failure of tick treatment (acaracide resistance). A total of 1600 ticks were collected from 20 farms located in three different regions of Abu Dhabi Emirate including Al Ain (n = 10), Al Dhafra (n = 5), and Abu Dhabi (n = 5). The ticks were subjected to an in vitro bioassay adult immersion test (AIT) modified with a discriminating dose (AIT-DD) against commercial preparations of Cypermethrin and Deltamethrin. A questionnaire was designed to collect metadata and information on farm management and the farm-level risk factors associated with routine farm practices relating to the treatment and control of tick and blood parasite infections in camels and small ruminant populations. Hyalomma anatolicum and Hyalomma dromedarii were identified among the collected ticks, with H. anatolicum being the most prevalent tick species (70%) in the present study. The test results of the in vitro bioassay revealed varied emerging resistance to both of the acaricides in the majority of the three regions; fully susceptible tick isolates with zero resistance to Deltamethrin were recorded in one farm at Al Ain and two farms in the Abu Dhabi region. A questionnaire analysis showed that the failure of tick treatment in farms varied with the presence or absence of vegetation areas, types of animal breeds, and management practices. This study reports the emergence of resistance in ticks to Cypermethrin and Deltamethrin across the Abu Dhabi Emirate, indicating a strict warning for the cautious use of acaricides. There is also a need to improve awareness about sound tick management and control practices among farm owners through a multidisciplinary approach adopting integrated pest management strategies that engage farmers, veterinarians, and policy makers.