The Lagoon of Venice (LoV) and the Gulf of Venice (GoV), two adjacent coastal Long Term Ecological Research (LTER) sites in the northern Adriatic Sea, represent a transitional/marine coupled ecosystem under the influence of regional and local factors. In this study, these sites were sampled on four dates from April 2016 to February 2017 for environmental DNA and relevant abiotic variables, aiming to assess the relative importance of habitat heterogeneity and connectivity in structuring the protist community. High Throughput Sequencing of V4-18S rRNA gene from 56 samples collected at seven stations produced ca 6 million reads, grouped into 7,336 Operational Taxonomic Units (OTUs) at 97% similarity, which were affiliated to protists belonging to 34 taxonomic groups. The whole community was dominated by Bacillariophyta, especially in spring-summer in the LoV, and by Dinophyta, mainly in the GoV. Ciliophora, Syndiniales, and Cryptophyceae were the next more abundant groups. The community structure varied across the seasons and was different in the two ecosystems, which shared 96% of the reads but showed a high proportion of OTUs distributed preferentially in one of the two sites (specialists) and a different partitioning of trophic categories. GoV specialists were mainly Dinophyceae (>56%), followed by Syndiniales and Bacillariophyta, while the LoV specialists were distributed among several groups, including Bacillariophyta, Syndiniales, Ciliophora, Cryptophyceae, and Trebouxiophyceae. The main abiotic drivers of the differences between protist communities were salinity and temperature, which however explained a minor part of the variance (17%), pointing at a higher relevance of biotic factors and inter-taxa relationships. This was more evident in the LoV, where the network analysis highlighted a higher number of OTUs’ connections than in the GoV. Overall, the metabarcoding approach allowed to depict the composition of the whole protist community in the lagoon and adjacent coastal waters with high resolution, revealing many taxa so far not reported in the area. In addition, despite no clear barrier to dispersal processes, differences in the relative abundance and temporal variability of local protist communities indicate that environmental heterogeneity, in these adjacent and connected ecosystems, can be strong enough to allow for ecological segregation.

Undaria pinnatifida (U. pinnatifida) is a highly invasive species and has caused concern all over the world because it has invaded coastal environments, has the potential to displace native species, significantly alters habitat for associated fauna, and disturbs navigation. Any attempt to eradicate it would be futile, owing to the elusive, microscopic gametophyte, and because the alga thrives in sites rich in anthropic activities. Venice Lagoon is the largest Mediterranean transitional environment and the spot of the highest introduction of non-indigenous species, including U. pinnatifida, which is removed as a waste. We demonstrated that polysaccharide extracts from U. pinnatifida have an anticoagulant effect on human blood in vitro and are not cytotoxic. The results obtained by PT (normal values 70-120%) and APTT (normal values 28-40s) assays were significantly prolonged by the polysaccharide extracts of U. pinnatifida, therefore algal extracts are ideal candidates as antithrombotic agents.

Seaweeds are attracting increasing attention as an alternative healthy food and renewable drugs source and as agents of climate change mitigation that provide essential ecosystem services. In this context, seaweeds represent marine resources capable of supporting and pursuing the objectives of the Sustainable Blue Economy and the Bio-Based Circular Economy. In this review, we analyze the state of seaweed bio-based products and research on the Mediterranean Sea from the last 20 years. Results of this analysis show a large number of investigations focusing on antimicrobial, antioxidant and anti-inflammatory activities compared to on biofuels and bioplastics. Attempts at seaweed farming, although generally very limited, are present in Israel and some North African countries. Lastly, we focus on the Italian situation—including research, companies and legislation on seaweed production—and we discuss gaps, perspectives and challenges for the potential development of a sustainable seaweed industry according to the Sustainable Blue Economy.

The cosmopolitan genus Ulva Linnaeus includes species of green macroalgae found in marine, brackish and some freshwater environments. Although there is a wide literature for the determination of Ulva taxa in Europe, they are among the most problematic algae to accurately identify, because they have few distinctive features, as well as a high intraspecific variation. At present, the knowledge of both diversity and distribution of the genus Ulva in the Mediterranean Sea is almost entirely based on morphological studies and there is only a few published papers dealing with molecular data. Tunisia has a key position in the Mediterranean and constitutes a transition area with a rich habitat diversity between eastern and western basins. The latest inventory of marine macrophytes dates back to 1987, updated in 1995. The aim of the present paper is to provide a molecular-assisted alpha taxonomy survey of Ulva spp. along Tunisian coasts, in comparison with a few Italian sites, using the tufA marker. Nine genetic species groups were resolved, including the non indigenous species Ulva ohnoi, newly reported for Tunisia. The actual picture of the taxonomy of Ulva spp. in the Mediterranean as a whole is far to be clarified and the present data on Tunisian collections aim to be a step towards its clarification. This paper is the first DNA barcoding study on green macroalgae in Tunisia and it contributes to add records to the Barcode Of Life Data Systems which are publically available.