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Dr. Ding (PhD) is currently working as a full-time researcher at the Lanzhou Veterinary Research Institute (LVRI), Chinese Academy of Agricultural Sciences (CAAS), China, Gansu province, Lanzhou City, PR China. He received his BSc from Gansu Agricultural University. He got his MSc and PhD from the institute he is currently working in. He has published 8 articles in reputable journals as principal and co-investigator. His current research of interest focuses on diagnostic methods, bioinformatics, the molecular pathogenic mechanism of animal viral diseases, pathogens and host interactions (Porcine Reproductive and Respiratory Syndrome (PRRS), Porcine Circovirus type 2 (PCV2), Foot-and-Mouth Disease (FMD).
An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with His-SUMO (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs.
Guoxiu Li; Ashenafi Wubshet; Yaozhong Ding; Qian Li; Junfei Dai; Yang Wang; Qian Hou; Jiao Chen; Bing Ma; Anna Szczotka-Bochniarz; Susan Szathmary; Yongguang Zhang; Jie Zhang. Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus. Viruses 2021, 13, 1005 .
AMA StyleGuoxiu Li, Ashenafi Wubshet, Yaozhong Ding, Qian Li, Junfei Dai, Yang Wang, Qian Hou, Jiao Chen, Bing Ma, Anna Szczotka-Bochniarz, Susan Szathmary, Yongguang Zhang, Jie Zhang. Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus. Viruses. 2021; 13 (6):1005.
Chicago/Turabian StyleGuoxiu Li; Ashenafi Wubshet; Yaozhong Ding; Qian Li; Junfei Dai; Yang Wang; Qian Hou; Jiao Chen; Bing Ma; Anna Szczotka-Bochniarz; Susan Szathmary; Yongguang Zhang; Jie Zhang. 2021. "Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus." Viruses 13, no. 6: 1005.
Virus infections are the root cause of epidemics in the world. Vaccines and antiviral agents have been the two important methods to control viral diseases; in recent times, RNA-mediated therapeutics and prevention have received much attention. In this review, we provide an overview of the current information regarding the use of vaccines, antiviral agents, and RNA-mediated methods in controlling or preventing viral infections. We stress specifically on the potential of existing RNA-mediated methods in clinical applications.
Yao-Zhong Ding; Jan-Liang Lv; Zhong-Wang Zhang; Xiao-Yuan Ma; Jie Zhang; Yong-Guang Zhang. The program of antiviral agents inhibits virus infection. Archives of Microbiology 2018, 200, 841 -846.
AMA StyleYao-Zhong Ding, Jan-Liang Lv, Zhong-Wang Zhang, Xiao-Yuan Ma, Jie Zhang, Yong-Guang Zhang. The program of antiviral agents inhibits virus infection. Archives of Microbiology. 2018; 200 (6):841-846.
Chicago/Turabian StyleYao-Zhong Ding; Jan-Liang Lv; Zhong-Wang Zhang; Xiao-Yuan Ma; Jie Zhang; Yong-Guang Zhang. 2018. "The program of antiviral agents inhibits virus infection." Archives of Microbiology 200, no. 6: 841-846.
Long noncoding (lnc)RNAs comprise a diverse group of transcripts including large intervening noncoding (linc)RNAs, natural antisense transcripts (NATs) and intronic lncRNAs. The functions and mechanisms of more than 200 lncRNAs have been studied in vitro and the results suggest that lncRNAs may be molecular markers of prognosis in cancer patients. Some lncRNAs can promote virus replication and allow escape from cytosolic surveillance to suppress antiviral immunity. For example, lncRNA can cause persistent infection by Theiler's virus, and microRNA (miR)-27a/b is important for efficient murine cytomegalovirus (MCMV) replication. The available evidence suggests that lncRNAs may be potential targets of novel antiviral drugs.
Yao-Zhong Ding; Zhong-Wang Zhang; Ya-Li Liu; Chong-Xu Shi; Jie Zhang; Yong-Guang Zhang. Relationship of long noncoding RNA and viruses. Genomics 2016, 107, 150 -154.
AMA StyleYao-Zhong Ding, Zhong-Wang Zhang, Ya-Li Liu, Chong-Xu Shi, Jie Zhang, Yong-Guang Zhang. Relationship of long noncoding RNA and viruses. Genomics. 2016; 107 (4):150-154.
Chicago/Turabian StyleYao-Zhong Ding; Zhong-Wang Zhang; Ya-Li Liu; Chong-Xu Shi; Jie Zhang; Yong-Guang Zhang. 2016. "Relationship of long noncoding RNA and viruses." Genomics 107, no. 4: 150-154.
The adaptation of the overall codon usage pattern of hepatitis C virus (HCV) to that of human is estimated by the synonymous codon usage value (RSCU). The synonymous codon usage biases for the translation initiation region (TIR) of this virus are also analyzed by calculation of usage fluctuation of each synonymous codon along the TIR (the first 30 codon sites of the whole coding sequence of HCV). As for the overall codon usage pattern of HCV, this virus has a significant tendency to delete the codons with CpG or TpA dinucleotides. Turning to the adaptation of the overall codon usage of HCV to that of human, over half part of codons has a similar usage pattern between this virus and human, suggesting that the host cellular environment of the overall codon usage pattern influences the formation of codon usage for HCV. In addition, there is no obvious phenomenon that the codons with relatively low energy tend to be highly selected in the TIR of HCV, suggesting that the synonymous codon usage patterns for the TIR of HCV might be not affected by the secondary structure of nucleotide sequence, however, the formation of synonymous codons usage in the TIR of HCV is influenced by the overall codon usage patterns of human to some degree.
Jian-Hua Zhou; Jun-Hong Su; Hao-Tai Chen; Jie Zhang; Li-Na Ma; Yao-Zhong Ding; Laszlo Stipkovits; Susan Szathmary; Zygmunt Pejsak; Yong-Sheng Liu. Clustering of low usage codons in the translation initiation region of hepatitis C virus. Infection, Genetics and Evolution 2013, 18, 8 -12.
AMA StyleJian-Hua Zhou, Jun-Hong Su, Hao-Tai Chen, Jie Zhang, Li-Na Ma, Yao-Zhong Ding, Laszlo Stipkovits, Susan Szathmary, Zygmunt Pejsak, Yong-Sheng Liu. Clustering of low usage codons in the translation initiation region of hepatitis C virus. Infection, Genetics and Evolution. 2013; 18 ():8-12.
Chicago/Turabian StyleJian-Hua Zhou; Jun-Hong Su; Hao-Tai Chen; Jie Zhang; Li-Na Ma; Yao-Zhong Ding; Laszlo Stipkovits; Susan Szathmary; Zygmunt Pejsak; Yong-Sheng Liu. 2013. "Clustering of low usage codons in the translation initiation region of hepatitis C virus." Infection, Genetics and Evolution 18, no. : 8-12.
The 3C protease of foot-and-mouth disease virus (FMDV) has a conserved amino acid sequence and is responsible for most cleavage in the viral polyprotein. The effects of the synonymous codon usage of FMDV 3C gene and tRNA abundance of the hosts on shaping different folding units (α-helix, β-strand and the coil) in the 3C protease were analyzed based on the structural information of the FMDV 3C protease from Protein Data Bank (PDB: 2BHG) and 210 genes of 3C for all serotypes of FMDV. The strong correlation between some codons usage and the specific folding unit in the FMDV 3C protease is found. As for the effect of translation speed caused by tRNA abundance on the formation of folding units, the codon positions with lowly abundant tRNA scatter in the 3C gene and there is the obvious fluctuation of tRNA abundance locating in the transition boundaries from the β-strand to the α-helix and the coil, respectively. However, codon positions with lowly abundant tRNA clustering into these boundaries are not found, suggesting that the adjustment of translation speed is likely also achieved by the single codon position with low tRNA abundance rather than a cluster. The observations can provide the information for insight into the role of the synonymous codon usage in the formation of 3C protease of FMDV and effect of the tRNA abundance of the hosts on this formation of protease.
Jian-Hua Zhou; Ya-Nan You; Hao-Tai Chen; Jie Zhang; Li-Na Ma; Yao-Zhong Ding; Zygmunt Pejsak; Yong-Sheng Liu. The effects of the synonymous codon usage and tRNA abundance on protein folding of the 3C protease of foot-and-mouth disease virus. Infection, Genetics and Evolution 2013, 16, 270 -274.
AMA StyleJian-Hua Zhou, Ya-Nan You, Hao-Tai Chen, Jie Zhang, Li-Na Ma, Yao-Zhong Ding, Zygmunt Pejsak, Yong-Sheng Liu. The effects of the synonymous codon usage and tRNA abundance on protein folding of the 3C protease of foot-and-mouth disease virus. Infection, Genetics and Evolution. 2013; 16 ():270-274.
Chicago/Turabian StyleJian-Hua Zhou; Ya-Nan You; Hao-Tai Chen; Jie Zhang; Li-Na Ma; Yao-Zhong Ding; Zygmunt Pejsak; Yong-Sheng Liu. 2013. "The effects of the synonymous codon usage and tRNA abundance on protein folding of the 3C protease of foot-and-mouth disease virus." Infection, Genetics and Evolution 16, no. : 270-274.
The synonymous codon usage patterns of open reading frame (ORF) in foot-and-mouth disease virus (FMDV), the similarity degree of the synonymous codon usage between this virus and the hosts and the genetic diversities of FMDV ORFs and the viral functional genes in viral ORF have been investigated by some simply statistical analyses. As for the synonymous codon usage of FMDV, some over-represented and under-represented codons have a similar usage in all seven serotypes. 33 out of 59 synonymous codons are similarly selected between FMDV ORF and the hosts. It is interesting that the overall codon usage pattern of the strains of serotype O isolated from pigs is different with that of strains of the same serotype isolated from non-pig origin, suggesting that the factor of pigs takes part in the formation of codon usage of FMDV serotype O. Projection of codon usage of nine viral functional genes onto the two-dimensional map represents that even though viral functional genes have various genetic diversities and each gene is not separated from each other based on seven serotypes, the codon usage patterns of VP2, 2C, 3A, 3C and 3D genes belonging to serotype O strains isolated from pigs are different with those of the same serotype strains from non-pig origin. In addition, the interaction between GC12% and GC3% of viral functional genes indicates that the codon usage patterns of VP1, VP2, 2B, 3A, 3C and 3D genes are influenced by mutation pressure from virus. Furthermore, distribution plots of ENC value vs. GC3% for viral function genes indicate that mutation pressure is not the only factor in the formation of codon usage of these genes. The results suggest that both the mutation pressure from virus and the translation selection from the hosts take part in the evolution process of viral functional genes of FMDV.
Jian-Hua Zhou; Zong-Liang Gao; Jie Zhang; Yao-Zhong Ding; Laszlo Stipkovits; Susan Szathmary; Zygmunt Pejsak; Yong-Sheng Liu. The analysis of codon bias of foot-and-mouth disease virus and the adaptation of this virus to the hosts. Infection, Genetics and Evolution 2013, 14, 105 -110.
AMA StyleJian-Hua Zhou, Zong-Liang Gao, Jie Zhang, Yao-Zhong Ding, Laszlo Stipkovits, Susan Szathmary, Zygmunt Pejsak, Yong-Sheng Liu. The analysis of codon bias of foot-and-mouth disease virus and the adaptation of this virus to the hosts. Infection, Genetics and Evolution. 2013; 14 ():105-110.
Chicago/Turabian StyleJian-Hua Zhou; Zong-Liang Gao; Jie Zhang; Yao-Zhong Ding; Laszlo Stipkovits; Susan Szathmary; Zygmunt Pejsak; Yong-Sheng Liu. 2013. "The analysis of codon bias of foot-and-mouth disease virus and the adaptation of this virus to the hosts." Infection, Genetics and Evolution 14, no. : 105-110.
Ovine adenovirus 287 (OAdV287) emerges as one of the most promising gene vectors resulting from its unique biological characteristics. To obtain a more detailed knowledge about the codon usage of OAdV287, a comparative study based on the codon usage of OAdV287 and the prototypes of human adenovirus serotypes 2 and 5 (HAdV2/5) was carried out. Some commonly used indices measuring the codon usage patterns, including effective number of codons, relative synonymous codon usage, and statistical methods, were adopted. Overall, OAdV287 had a more biased and conservative codon usage pattern than that of HAdV2/5. Both mutation pressure and natural selection played important roles in shaping the codon usage patterns of these three adenoviruses. All the preference codons of OAdV287 had A/U ends and were totally different from those of sheep and humans; however, the preference codons of HAdV2/5 mostly had G/C ends and were mostly coincident with those of sheep and humans. The codon usage analysis in this study supplies some clues for further comprehending the unique biological characteristics of OAdV287 as gene vectors.
Yuan-Xing Gu; Jie Zhang; Jian-Hua Zhou; Feng Zhao; Wen-Qian Liu; Meng Wang; Hao-Tai Chen; Li-Na Ma; Yao-Zhong Ding; Yong-Sheng Liu. Comparative Analysis of Ovine Adenovirus 287 and Human Adenovirus 2 and 5 Based on Their Codon Usage. DNA and Cell Biology 2012, 31, 360 -366.
AMA StyleYuan-Xing Gu, Jie Zhang, Jian-Hua Zhou, Feng Zhao, Wen-Qian Liu, Meng Wang, Hao-Tai Chen, Li-Na Ma, Yao-Zhong Ding, Yong-Sheng Liu. Comparative Analysis of Ovine Adenovirus 287 and Human Adenovirus 2 and 5 Based on Their Codon Usage. DNA and Cell Biology. 2012; 31 (3):360-366.
Chicago/Turabian StyleYuan-Xing Gu; Jie Zhang; Jian-Hua Zhou; Feng Zhao; Wen-Qian Liu; Meng Wang; Hao-Tai Chen; Li-Na Ma; Yao-Zhong Ding; Yong-Sheng Liu. 2012. "Comparative Analysis of Ovine Adenovirus 287 and Human Adenovirus 2 and 5 Based on Their Codon Usage." DNA and Cell Biology 31, no. 3: 360-366.
To give a new perspective on the codon usage of the hepatitis C virus (HCV) and the factors accounting for shaping the codon usage pattern of the virus, the relative synonymous codon usage (RSCU) values, aromaticity and hydrophobicity of each polyprotein of the virus, effective number of codons (ENC) values and nucleotide contents were calculated to implement a comparative analysis to evaluate the dynamics of the virus evolution. The RSCU values of each codon of 144 HCV ORFs indicated that all abundant codons were C/G-ended codons. The plots of principal component analysis based on sub-genotype of HCV indicated that sub-genotype 1a and 1b separated clearly on the axis of f2 suggesting that the codon usage bias between sub-genotype 1a and 1b strains was different. By comparing the codon usage between HCV and human cells, we found that the synonymous codon usage pattern of HCV was a mixture of coincidence and antagonism to that of host cells. The characteristics of the synonymous codon usage patterns and nucleotide contents of HCV, and the correlation analysis between GC(3s), GC(1,2s), GC% (ORF), GC% (5'-UTR), GC% (3'-UTR), aromaticity, hydrophobicity and ENC value, respectively, indicated that mutational pressure was the dominant factor accounting for the codon usage variation and selection pressure also accounted for HCV codon usage pattern.
Jin-Song Hu; Qin-Qin Wang; Jie Zhang; Hao-Tai Chen; Zhi-Wen Xu; Ling Zhu; Yao-Zhong Ding; Li-Na Ma; Kai Xu; Yuan-Xing Gu; Yong-Sheng Liu. The characteristic of codon usage pattern and its evolution of hepatitis C virus. Infection, Genetics and Evolution 2011, 11, 2098 -2102.
AMA StyleJin-Song Hu, Qin-Qin Wang, Jie Zhang, Hao-Tai Chen, Zhi-Wen Xu, Ling Zhu, Yao-Zhong Ding, Li-Na Ma, Kai Xu, Yuan-Xing Gu, Yong-Sheng Liu. The characteristic of codon usage pattern and its evolution of hepatitis C virus. Infection, Genetics and Evolution. 2011; 11 (8):2098-2102.
Chicago/Turabian StyleJin-Song Hu; Qin-Qin Wang; Jie Zhang; Hao-Tai Chen; Zhi-Wen Xu; Ling Zhu; Yao-Zhong Ding; Li-Na Ma; Kai Xu; Yuan-Xing Gu; Yong-Sheng Liu. 2011. "The characteristic of codon usage pattern and its evolution of hepatitis C virus." Infection, Genetics and Evolution 11, no. 8: 2098-2102.
In this study, an abundant (A + U)% and low codon bias were revealed in duck hepatitis virus type 1 (DHV-1) and the new serotype strains isolated from Taiwan, South Korea and Mainland China (DHV-N). The general correlation between base composition and codon usage bias suggests that mutational pressure rather than natural selection is the main factor that determines the codon usage bias in these samples. By comparative analysis of the codon usage patterns of 40 ORFs of DHV, we found that all of DHV-1 strains grouped in genotype C; the DHV-N strains isolated in South Korea and China clustered into genotypes B; and the DHV-N strains isolated from Taiwan clustered into genotypes A. The findings revealed that more than one subtype of DHV-1 circulated in East Asia. Furthermore, the results of phylogenetic analyses based on RSCU values and Clustal W method indicated obvious phylogenetic congruities. This suggested that better genome consistency of DHV may exist in nature and phylogenetic analyses based on RSCU values maybe a good method in classifying genotypes of the virus. Our work might give some clues to the features and some evolutionary information of DHV.
Meng Wang; Jie Zhang; Jian-Hua Zhou; Hao-Tai Chen; Li-Na Ma; Yao-Zhong Ding; Wen-Qian Liu; Yuan-Xing Gu; Feng Zhao; Yong-Sheng Liu. Analysis of codon usage in type 1 and the new genotypes of duck hepatitis virus. Biosystems 2011, 106, 45 -50.
AMA StyleMeng Wang, Jie Zhang, Jian-Hua Zhou, Hao-Tai Chen, Li-Na Ma, Yao-Zhong Ding, Wen-Qian Liu, Yuan-Xing Gu, Feng Zhao, Yong-Sheng Liu. Analysis of codon usage in type 1 and the new genotypes of duck hepatitis virus. Biosystems. 2011; 106 (1):45-50.
Chicago/Turabian StyleMeng Wang; Jie Zhang; Jian-Hua Zhou; Hao-Tai Chen; Li-Na Ma; Yao-Zhong Ding; Wen-Qian Liu; Yuan-Xing Gu; Feng Zhao; Yong-Sheng Liu. 2011. "Analysis of codon usage in type 1 and the new genotypes of duck hepatitis virus." Biosystems 106, no. 1: 45-50.
To investigate the codon usage pattern of the contexts flanking 11 cleavage sites of foot-and-mouth disease virus (FMDV) polyprotein, the codon usage model of the corresponding codon position and the synonymous codon usage in the target contexts of 66 strains were characterized by two simple methods based on the relative synonymous codon usage value. The synonymous codons usage pattern was also compared between this virus and two species of hosts (cattle and domestic pig). It is indicated that FMDV bore a general resemblance to the hosts in terms of the synonymous codon usage pattern. This feature may help FMDV to utilize translational resources of host efficiently. The two amino acid residues constituting each cleavage site contain at least one conserved residue. It was noticed that the codon usage model with the strong bias appeared in some specific positions in the target contexts, and the under-represented synonymous codons, AUA for Ile, CUA for Leu, UUA for Leu and GUA for Val, are preferentially used in these positions. These under-represented synonymous codons likely play role in regulating the translation rate and influencing the secondary structure of the contexts flanking the cleavage sites.
Jian-Hua Zhou; Jie Zhang; Hao-Tai Chen; Li-Na Ma; Yao-Zhong Ding; Zygmunt Pejsak; Yong-Sheng Liu. The codon usage model of the context flanking each cleavage site in the polyprotein of foot-and-mouth disease virus. Infection, Genetics and Evolution 2011, 11, 1815 -1819.
AMA StyleJian-Hua Zhou, Jie Zhang, Hao-Tai Chen, Li-Na Ma, Yao-Zhong Ding, Zygmunt Pejsak, Yong-Sheng Liu. The codon usage model of the context flanking each cleavage site in the polyprotein of foot-and-mouth disease virus. Infection, Genetics and Evolution. 2011; 11 (7):1815-1819.
Chicago/Turabian StyleJian-Hua Zhou; Jie Zhang; Hao-Tai Chen; Li-Na Ma; Yao-Zhong Ding; Zygmunt Pejsak; Yong-Sheng Liu. 2011. "The codon usage model of the context flanking each cleavage site in the polyprotein of foot-and-mouth disease virus." Infection, Genetics and Evolution 11, no. 7: 1815-1819.
A short linear peptide was designed according to the antigenic site analysis of VP1 protein of foot-and-mouth virus (FMDV) serotype O and synthesized as the peptide immunogen. The peptide, which covers the region from amino acid 133 to 160 of VP1 of FMDV, was linked to the N-terminal cysteine and conjugated with the carrier protein of keyhole limpet hemocyanin (KLH). Normal 6- to 8-week-old BALB/c mice were immunized with the 20 μg dose conjugated peptide antigen four times. The splenocytes from the immunized mice were fused with SP2/0 mouse myeloma cells, and positive hybridomas were selected by indirect enzyme-linked immunosorbent assay (ELISA) and subcloned four times with limiting dilution. Five stable hybridoma cell lines, designated as 4F9, 1B11, 1E10, 1D4, and 4B8, were obtained. Isotyping of all obtained MAbs indicated that the MAbs of 4F9, 1E10, and 4B8 belonged to IgG2b; the 1B11 and 1D4 belonged to IgG1 and IgM, respectively. The micro-neutralization test indicated that the MAbs of 4F9, 4B8, and 1B11 were capable of neutralizing FMDV serotype O with neutralization indices ranging from 1.81 to 2.11. These results suggest that linear synthetic peptide conjugate can elicit antibodies against native FMDV virus and can be used as an alternative immunogen for production of MAbs with exact epitope.
Lina Ma; Yong-Sheng Liu; Yao-Zhong Ding; Hao-Tai Chen; Jian-Hua Zhou; Wen-Qian Liu; Meng Wang; Jie Zhang. Preparation and Characterization of Neutralizing Monoclonal Antibodies Against FMDV Serotype O with Synthetic Peptide Antigen. Hybridoma 2010, 29, 409 -412.
AMA StyleLina Ma, Yong-Sheng Liu, Yao-Zhong Ding, Hao-Tai Chen, Jian-Hua Zhou, Wen-Qian Liu, Meng Wang, Jie Zhang. Preparation and Characterization of Neutralizing Monoclonal Antibodies Against FMDV Serotype O with Synthetic Peptide Antigen. Hybridoma. 2010; 29 (5):409-412.
Chicago/Turabian StyleLina Ma; Yong-Sheng Liu; Yao-Zhong Ding; Hao-Tai Chen; Jian-Hua Zhou; Wen-Qian Liu; Meng Wang; Jie Zhang. 2010. "Preparation and Characterization of Neutralizing Monoclonal Antibodies Against FMDV Serotype O with Synthetic Peptide Antigen." Hybridoma 29, no. 5: 409-412.
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 101 50% egg infection dose (EID50) per 50 μl of titrated viruses and no cross-reaction of IBV RT-LAMP was found when tested with other viruses including Newcastle disease virus (NDV), avian reovirus (ARV), and infectious laryngotrachietis virus (ILTV) due to their mismatch with IBV RT-LAMP primers. A total of 187 clinical tissues samples (88 blood, 62 kidney and 37 lung) were evaluated and compared to conventional RT-PCR. The sensitivity of RT-LAMP and RT-PCR assays for detecting IBV RNA in clinical specimens was 99.5% and 98.4%, respectively. These findings showed that the RT-LAMP assay has potential usefulness for rapid and sensitive diagnosis in outbreak of IBV.
Hao-Tai Chen; Jie Zhang; Yan-Ping Ma; Li-Na Ma; Yao-Zhong Ding; Xiang-Tao Liu; Xue-Peng Cai; Yong-Guang Zhang; Yong-Sheng Liu. Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues. Molecular and Cellular Probes 2010, 24, 104 -106.
AMA StyleHao-Tai Chen, Jie Zhang, Yan-Ping Ma, Li-Na Ma, Yao-Zhong Ding, Xiang-Tao Liu, Xue-Peng Cai, Yong-Guang Zhang, Yong-Sheng Liu. Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues. Molecular and Cellular Probes. 2010; 24 (2):104-106.
Chicago/Turabian StyleHao-Tai Chen; Jie Zhang; Yan-Ping Ma; Li-Na Ma; Yao-Zhong Ding; Xiang-Tao Liu; Xue-Peng Cai; Yong-Guang Zhang; Yong-Sheng Liu. 2010. "Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues." Molecular and Cellular Probes 24, no. 2: 104-106.
Monoclonal antibodies (MAbs) against prion protein (PrP) are powerful tools for diagnosis and research in transmissible spongiform encephalopathies. Ten MAbs to recombinant/native cellular PrP (PrPc) in mammals were prepared with a simple method and identified in detail. Normal BALB/c mice were immunized with the recombinant bovine mature PrP (rbomPrP) and PrP27-30 (rboPrP27-30) expressed in Escherichia coli. The immunized splenocytes were fused with SP2/0 mouse myeloma cells, and positive hybridomas were selected by indirect enzyme-linked immunosorbent assay (ELISA). The characterizations of these MAbs, such as Ig, Ig subclass, titer, affinity index, specificity, epitopes recognized, and binding to recombinant/native PrPc of cattle, sheep, or human beings, were evaluated by Western blotting and indirect or sandwich ELISA. Ten MAbs could be divided into five groups depending on the results of indirect ELISA additivity test and their reaction to E. coli-expressed truncated-PrPs. Isotyping of the MAbs revealed that they belong to IgG1, IgG2a, and IgG2b subclass. Their indirect ELISA titers were between 10(3) and 10(6). Affinity constants were between 10(9) and 10(12) M(-1). Ten MAbs specifically reacted with the rbomPrP, without binding to prion-like protein Doppel and the lysates of E. coli. These MAbs could also respond to the recombinant mature PrP (rmPrP) of sheep and human beings. Also of interest was the ability of the MAbs to bind with dimer of rmPrP and PrP extracted from the brain tissue of cattle or sheep. We conclude that anti-PrP MAbs successfully prepared with a simple method could potentially be useful in mammalian prion research.
Yong-Sheng Liu; Yao-Zhong Ding; Jie Zhang; Hao-Tai Chen; Xiao-Ling Zhu; Xue-Peng Cai; Xiang-Tao Liu; Qing-Ge Xie. Simple Method of Monoclonal Antibody Production Against Mammalian Cellular Prion Protein. Hybridoma 2010, 29, 37 -43.
AMA StyleYong-Sheng Liu, Yao-Zhong Ding, Jie Zhang, Hao-Tai Chen, Xiao-Ling Zhu, Xue-Peng Cai, Xiang-Tao Liu, Qing-Ge Xie. Simple Method of Monoclonal Antibody Production Against Mammalian Cellular Prion Protein. Hybridoma. 2010; 29 (1):37-43.
Chicago/Turabian StyleYong-Sheng Liu; Yao-Zhong Ding; Jie Zhang; Hao-Tai Chen; Xiao-Ling Zhu; Xue-Peng Cai; Xiang-Tao Liu; Qing-Ge Xie. 2010. "Simple Method of Monoclonal Antibody Production Against Mammalian Cellular Prion Protein." Hybridoma 29, no. 1: 37-43.
The aim of this study was to develop a rapid, cost-saving triple reverse transcription polymerase chain reaction (triple RT-PCR) for subtyping H9N2 avian influenza viruses (AIVs). The three primer pairs for amplification of target sequences of nucleoprotein (NP), hemagglutinin (HA) and neuraminidase (NA) genes, respectively, were designed for subtyping the viruses in the triple RT-PCR. The sensitivity of triple RT-PCR was found to be 10(2) copies per reaction for each of NP, H9 and N2 gene. The specificity tests indicated that all of NP, HA and NA genes were positive for H9N2, only NP gene was positive for H5N1 and H1N1 AIVs, and the results were negative for the other avian viruses including Newcastle disease virus, infectious bronchitis virus, infectious bursal disease virus, duck hepatitis virus and avian encephalomyelitis virus. A total of 112 clinical samples were evaluated by the assay and the results showed that the sensitivity and specificity of triple RT-PCR were in accordance with the virus isolation. In conclusion, this method is rapid and cost-effective making it feasible and attractive for large-scale epidemiological investigation of H9N2 influenza virus.
Hao-Tai Chen; Jie Zhang; Li-Na Ma; Yan-Ping Ma; Yao-Zhong Ding; Meng Wang; Xiang-Tao Liu; Yong-Guang Zhang; Yong-Sheng Liu. Rapid subtyping of H9N2 influenza virus by a triple reverse transcription polymerase chain reaction. Journal of Virological Methods 2009, 158, 58 -62.
AMA StyleHao-Tai Chen, Jie Zhang, Li-Na Ma, Yan-Ping Ma, Yao-Zhong Ding, Meng Wang, Xiang-Tao Liu, Yong-Guang Zhang, Yong-Sheng Liu. Rapid subtyping of H9N2 influenza virus by a triple reverse transcription polymerase chain reaction. Journal of Virological Methods. 2009; 158 (1-2):58-62.
Chicago/Turabian StyleHao-Tai Chen; Jie Zhang; Li-Na Ma; Yan-Ping Ma; Yao-Zhong Ding; Meng Wang; Xiang-Tao Liu; Yong-Guang Zhang; Yong-Sheng Liu. 2009. "Rapid subtyping of H9N2 influenza virus by a triple reverse transcription polymerase chain reaction." Journal of Virological Methods 158, no. 1-2: 58-62.
The usefulness of reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid pre-clinical detection of classical swine fever virus (CSFV) infection was evaluated. The RT-LAMP reaction could be finished in 60 min under isothermal condition at 65 °C by employing a set of four primers targeting the 5′ untranslated region of CSFV. The RT-LAMP assay of CSFV showed higher sensitivities than that of RT-PCR, with a detection limit of 5 copies per reaction. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, Japanese encephalitis virus, and porcine reproductive and respiratory syndrome virus. The detection rates of CSFV RT-LAMP, RT-PCR and virus isolation for samples including blood, tonsil, nasal and rectal swabs from uninoculated pigs without any clear clinical symptom were 89%, 78% and 71%, respectively. Furthermore, all of the assays showed higher sensitivity for blood and tonsil swabs samples than nasal and rectal swabs. These results indicate that the CSFV RT-LAMP assay is a valuable tool for its rapid, cost-effective detection and has potential usefulness for rapid pre-clinical detection and surveillance of classical swine fever in developing countries.
Hao-Tai Chen; Jie Zhang; Li-Na Ma; Yan-Ping Ma; Yao-Zhong Ding; Xiang-Tao Liu; Lei Chen; Yong-Guang Zhang; Yong-Sheng Liu. Rapid pre-clinical detection of classical swine fever by reverse transcription loop-mediated isothermal amplification. Molecular and Cellular Probes 2009, 23, 71 -74.
AMA StyleHao-Tai Chen, Jie Zhang, Li-Na Ma, Yan-Ping Ma, Yao-Zhong Ding, Xiang-Tao Liu, Lei Chen, Yong-Guang Zhang, Yong-Sheng Liu. Rapid pre-clinical detection of classical swine fever by reverse transcription loop-mediated isothermal amplification. Molecular and Cellular Probes. 2009; 23 (2):71-74.
Chicago/Turabian StyleHao-Tai Chen; Jie Zhang; Li-Na Ma; Yan-Ping Ma; Yao-Zhong Ding; Xiang-Tao Liu; Lei Chen; Yong-Guang Zhang; Yong-Sheng Liu. 2009. "Rapid pre-clinical detection of classical swine fever by reverse transcription loop-mediated isothermal amplification." Molecular and Cellular Probes 23, no. 2: 71-74.