Climate change has significantly influenced the spread of waterborne diseases (WBDs), which affect environmental quality and human life. The impact of climate change is greatest in developing countries, especially in the Association of Southeast Asian Nations (ASEAN) countries. Vibrio cholerae, a waterborne pathogen, is most susceptible to and most prevalent during severe climatic changes. The Philippines is regularly exposed to tropical cyclones, such as Bopha in 2012 and Haiyan in 2013, because of its geographical location, while Cyclone Nargis in 2008 caused over 95% of the damage and casualties seen in the preceding two decades in Myanmar. Therefore, implementing policies to adjust to these climate changes and to safeguard their citizens from the effects of WBDs is imperative for ASEAN countries. This study aimed to (1) investigate the effects of climate change on health and to understand the policy requirements to prevent or minimize its negative impact and (2) explore the link between the Sustainable Development Goals (SDGs) and the effects of climate change on WBDs to determine perspectives for global sustainability. The framework of the SDGs should be adapted to ASEAN countries to improve legislation, laws, and regulations on climate-related health issues. Efficient collaboration among scientists, researchers, health professionals, and policymakers will assist in addressing the problems associated with the impact of climate change on WBDs in ASEAN countries.

The nitrogen cycle is a biogeochemical cycle primarily associated with the microbial activity that occurs in various environments, including soil. Various genes related to the nitrogen cycle have been studied for different purposes by many researchers; therefore, the polymerase chain reaction (PCR) conditions and gene compositions differ among reports, making comparisons difficult. In this study, we compare the PCR methods to amplify 13 nitrogen cycle-related genes (amo (amoA and amoB), norB (cnorB and qnorB), hzs, napA, narG, nifH, nirK, nirS, nosZ, nrfA, and nxrA) in the soil samples collected from four land use types and selected a method with excellent applicability. However, the PCR method for five nitrogen cycle-related genes (amoC, hao, hzo, nirB, and nxrB) could not be presented. In addition, the nitrogen cycle-related genes from the four land use types (field, forest, bare land, and grassland) and the seasonally collected samples were analyzed and discussed. In the grassland samples, all the nitrogen cycle-related genes reviewed were amplified. These results vary from those of the field, forest, and bare land samples, and it was estimated that grassland, among the land use types, could play an important role in the nitrogen cycle in soil. However, an association between the seasons and the rainy season was not confirmed. Thus, this study may be used for future research in various fields related to the nitrogen cycle.