There is a recent and growing interest in the study and application of non‐ Saccharomyces yeasts, mainly in fermented foods. Numerous publications and patents show the importance of these yeasts. However, a fundamental issue in studying and applying them is to ensure an appropriate preservation scheme that allows to the non‐ Saccharomyces yeasts conserve their characteristics and fermentative capabilities by long periods of time. The main objective of this review is to present and analyze the techniques available to preserve these yeasts (by conventional and non‐conventional methods), in small or large quantities for laboratory or industrial applications, respectively. Wine fermentation is one of the few industrial applications of non‐ Saccharomyces yeasts, but the preservation stage has been a major obstacle to achieve a wider application of these yeasts. This review considers the preservation techniques, and clearly defines parameters such as culturability, viability, vitality and robustness. Several conservation strategies published in research articles as well as patents are analyzed, and the advantages and disadvantages of each technique used are discussed. Another important issue during conservation processes is the stress to which yeasts are subjected at the time of preservation (mainly oxidative stress). There is little published information on the subject for non‐ Saccharomyces yeast, but it is a fundamental point to consider when designing a preservation strategy.

Selenium nanoparticles (SeNPs) are currently receiving attention for controlling plant pathogenic microorganisms, are expected to be especially effective against the genus Phytophthora, and show high anti-oomycete activity. SeNPs synthesized with plant extracts have shown low toxicity, high bioavailability, and mechanisms of action that alter cellular integrity and damage key components of phytopathogen metabolism, causing denaturation and cell death. The aim of this study was to evaluate the inhibitory activity of SeNPs on mycelial growth and the development of reproductive structures in Phytophthora capsici in vitro. Different concentrations of SeNPs (0 to 400 µg/mL) in culture media were used to analyze mycelial growth, sporangium formation, zoospores, and germination of the germ tube. To explain the changes in morphology and development of P. capsici, increased relative conductance and activation of glycerol synthesis were related to osmotic stress and damage to membrane permeability. In addition, SeNPs inhibited the production of exopolysaccharides (EPSs), which are compounds associated with pathogen virulence. A lower accumulation of its biomass evidences alterations in the oomycete growth. The percentage of inhibition of mycelial growth increased with higher SeNP concentrations and incubation time, reaching 100% growth inhibition at 300 and 400 µg/mL. A concentration-dependent reduction in the number of spores, sporangia, and zoospore germination was observed. Concentrations of 50 and 100 µg/mL of SeNPs reduced biomass production by 30%. The increase in glycerol levels indicated an osmoregulatory response to SeNP-induced stress. Also, the increase in electrical conductivity suggested plasma membrane damage, which supports the potential of SeNPs as antifungal agents by inducing cell disruption and structural damage in P. capsici. These results provide new knowledge on the in vitro mechanism of action of SeNPs against P. capsici and offer a new biological alternative for the control of diseases caused by oomycetes.

Agro-industrial wastes can be used to obtain high-value compounds rich in antioxidant and antimicrobial activity. This study aimed to compare different extraction techniques for the recovery of sugars, antioxidants, and antimicrobial compounds from brewer’s spent grain (BSG), blue agave bagasse (BAB), spoiled blackberries (BB), and raspberries (RB). Aqueous (AQ), enzymatic (E), chemical-enzymatic (CE), and hydroalcoholic (EOH) extractions were assessed, and sugars, phenolics, flavonoids, and anthocyanin contents were quantified. Antioxidant activity of the extracts was evaluated using the ABTS and DPPH assays, and antimicrobial activity was tested against three yeasts and six bacteria. The CE process gave the highest total and reducing sugars content for the four residues tested, and the highest antioxidant activity, phenolics, flavonoids and anthocyanin content for BAB and BSG. Regarding BB and RB, the best treatment to obtain total and reducing sugars and antioxidant activity with ABTS was CE; the highest content of anthocyanins, phenolic, flavonoids and antioxidant activity with DPPH was obtained with EOH treatment. CE extracts of BSG and RB showed the highest inhibition against the strains studied. Results show that BSG, BB, and RB can be a source of antioxidants and antimicrobial compounds for the food and pharmaceutical industries. Depending on the desired application and component of interest, one of the extraction techniques evaluated here could be used.

This study investigated the potential of wastepaper hydrolysate as a sustainable and low-cost carbon source for single-cell oil and protein production, attending to the growing need for alternative feedstocks and waste management strategies. Wastepaper, characterized by its high carbohydrate content, was subjected to enzymatic and chemo-enzymatic treatments for carbohydrate release. The chemo-enzymatic treatment performed better, yielding 65.3 g L⁻¹ of fermentable sugars. Sixty-two yeast strains were screened for single-cell oil accumulation, identifying Rhodotorula mucilaginosa M1K4 as the most advantageous oleaginous yeast. M1K4 lipid production was optimized in liquid culture, and its fatty acid profile was analyzed, showing a high content of industrially valuable fatty acids, particularly palmitic (28%) and oleic (51%). Batch-culture of M1K4 in a 3 L reactor demonstrated the strain's ability to utilize wastepaper hydrolysate as a carbon source, with dry cell weight, total lipid and protein production of 17.7 g L⁻¹, 4.5 g L⁻¹, and 2.1 g L⁻¹, respectively. Wastepaper as a substrate provides a sustainable solution for waste management and bioproduction. This research highlights the potential of R. mucilaginosa for lipid and protein production from wastepaper hydrolysate.