In the last century, large watersheds in Southern Europe have been impacted by a combination of anthropogenic and climatic pressures, which have rapidly evolved to change the ecological status of freshwater and coastal systems. A comparative analysis was performed for Ebro, Rhône, Po and Danube rivers, to investigate if they exhibited differential dynamics in hydrology and water quality that can be linked to specific human and natural forces acting at sub-continental scales. Flow regime series were analyzed from daily to multi-decadal scales, considering frequency distributions, trends (Mann–Kendall and Sen tests) and discontinuities (SRSD Method). River loads of suspended matter, nutrients and organic matter and the eutrophication potential of river nutrients were estimated to assess the impact of river loads on adjacent coastal areas. The decline of freshwater resources largely impacted the Ebro watershed on annual (−0.139 km³ yr⁻¹) and seasonal (−0.4% yr⁻¹) scales. In the other rivers, only spring–summer showed significant decreases of the runoff coupled to an exacerbated flow variability (0.1–0.3% yr⁻¹), which suggested the presence of an enhanced regional climatic instability. Discontinuities in annual runoff series (every 20–30 years) indicated a similar long-term evolution of Rhône and Po rivers, differently from Ebro and Danube. Higher nutrient concentrations in the Ebro and Po (+50%) compared to Rhône and Danube and distinct stoichiometric nutrient ratios may exert specific impacts on the growth of plankton biomass in coastal areas. The overall decline of inorganic phosphorus in the Rhône and Po (since the 1980s) and the Ebro and Danube (since the 1990s) mitigated the eutrophication in coastal ecosystems inducing, however, a phase in which the role of organic phosphorus loads (Po > Danube > Rhône > Ebro) on coastal productivity could be more relevant. Overall, the study showed that the largest South European watersheds are differently impacted by anthropogenic and climatic forces and that this will influence their vulnerability to future changes of flow regime and water quality.

Eutrophication, driven by excessive nutrient enrichment from sources like agricultural runoff, industrial discharge, and urbanisation, has severely impacted the Black Sea since the 1980s. This study aimed to assess eutrophication dynamics in the Romanian Exclusive Economic Zone from 2020 to 2022 using the Black Sea Eutrophication Assessment Tool (BEAST), an integrated approach to the causes and effects of eutrophication. Data were collected from 68 stations during five oceanographic expeditions, analysing 617 water samples for nutrients, chlorophyll a, zooplankton species Noctiluca scintillans, and dissolved oxygen. Additionally, 179 zoobenthic and 251 phytobenthic community samples were collected. The results indicate that coastal waters exhibit higher nutrient levels and algal blooms compared to offshore waters, necessitating significant reductions in nutrient concentrations to achieve good environmental status. In transitional waters, within the Danube Delta Biosphere Reserve, a 55% reduction in inorganic phosphorus and a 43% reduction in inorganic nitrogen concentrations are required, while coastal waters need reductions of 38% and 37%, respectively. The study highlights the need for improved wastewater treatment, stricter agricultural runoff controls, and continuous monitoring. Effective ecosystem-based management strategies, integrated coastal zone management, and international cooperation are essential to mitigate eutrophication and promote the long-term health of the Black Sea ecosystem.

Heavy metals are among the most persistent and bioaccumulative pollutants in marine ecosystems, posing significant toxicological threats to fish via complex molecular and cellular disruptions. This review synthesizes current knowledge on the cascade of mechanistic responses in marine fish following HM exposure, which includes oxidative stress, modulation of antioxidant responses, activation of detoxification systems, DNA damage, inflammation, apoptosis, neuroendocrine disruption, and ultimately, cellular energy imbalance. In addition to established pathways, the review highlights recent advances in mechanistic understanding and biomarker development, including cellular stress responses, epigenetic regulation, metal homeostasis mechanisms, and novel molecular indicators. These mechanisms support the development of an integrated biomarker framework that combines classical indicators (e.g., antioxidant enzymes, metallothionein) with next-generation endpoints (e.g., miRNA profiles, gene-level responses of metal transporters or stress chaperones, epigenetic alterations). The interpretation of biomarker responses requires consideration of the exposure context, environmental variables, and physiological status to ensure accurate assessment of sublethal toxicity in field settings. By bridging mechanistic understanding with biomonitoring relevance, this review provides a comprehensive foundation for advancing molecular tools in pollution monitoring and risk assessment. Special emphasis is placed on biomarkers specific to heavy metal exposure, enhancing their diagnostic value relative to general stress indicators.

PERSEUS project aims to identify the most relevant pressures exerted on the ecosystems of the Southern European Seas (SES), highlighting knowledge and data gaps that endanger the achievement of SES Good Environmental Status (GES) as mandated by the Marine Strategy Framework Directive (MSFD). A complementary approach has been adopted, by a meta-analysis of existing literature on pressure/impact/knowledge gaps summarized in tables related to the MSFD descriptors, discriminating open waters from coastal areas. A comparative assessment of the Initial Assessments (IAs) for five SES countries has been also independently performed. The comparison between meta-analysis results and IAs shows similarities for coastal areas only. Major knowledge gaps have been detected for the biodiversity, marine food web, marine litter and underwater noise descriptors. The meta-analysis also allowed the identification of additional research themes targeting research topics that are requested to the achievement of GES.

This study investigates the long-term seasonal shifts in nutrient dynamics and eutrophication processes in the Romanian Black Sea coastal waters using multi-decadal data (1960/1976/1980–2023). The findings highlight significant seasonal and interannual changes, revealing a progressive rise in seawater temperature, declining oxygen concentrations, and notable shifts in nutrient stoichiometry, particularly an increasing nitrogen–phosphorus (N:P) ratio. These changes are closely associated with increased occurrences of harmful algal blooms (Noctiluca scintillans), emphasizing the complex relationship between warming, nutrient and dissolved oxygen cycles, and biological activity. Seasonal patterns show that prolonged warmer periods, especially during autumn, exacerbate oxygen depletion and nutrient imbalances, with implications for marine life and food webs. The study underscores the importance of targeted nitrogen reduction strategies, including optimized fertilizer use, improved wastewater treatment, and the establishment of buffer zones to minimize land-based nutrient inputs. Regional cooperation and integrated coastal management aligned with the Marine Strategy Framework Directive are essential for mitigating eutrophication. The results provide critical insights into the impacts of climate change on the Black Sea ecosystems. This research contributes to global efforts under SDG 13 (Climate Action), SDG 14 (Life Below Water), and SDG 3 (Good Health and Well-being), addressing the key challenges to marine biodiversity, water quality, and ecosystem sustainability.