Nanotheranostics—where nanoscale materials serve both diagnostic and therapeutic functions—are rapidly transforming gene therapy by tackling critical delivery challenges. This review explores the design and engineering of various nanoparticle systems (lipid-based, polymeric, inorganic, and hybrid) to enhance stability, targeting, and endosomal escape of genetic payloads. We discuss how real-time imaging capabilities integrated into these platforms enable precise localization and controlled release of genes, improving treatment efficacy while reducing off-target effects. Key strategies to overcome delivery barriers (such as proton sponge effect and photothermal disruption) and to achieve nuclear localization are highlighted, along with recent advances in stimuli-responsive systems that facilitate spatiotemporal control of gene expression. Clinical trials and preclinical studies demonstrate the expanding role of nanotheranostics in managing cancer, inherited disorders, and cardiovascular and neurological diseases. We further address regulatory and manufacturing hurdles that must be overcome for the widespread clinical adoption of nanoparticle-based gene therapies. By synthesizing recent progress and ongoing challenges, this review underscores the transformative potential of nanotheranostics for effective, targeted, and image-guided gene delivery.

The food and beverage industry is constantly evolving, and consumers are increasingly searching for premium products that not only offer health benefits but a pleasant taste. A viable strategy to accomplish this is through the altering of sensory profiles through encapsulation of compounds with unique flavors. We used this approach here to examine how brewing in the presence of yeast cells encapsulated in alginate affected the sensory profile of beer wort. Initial tests were conducted for various combinations of sodium alginate and calcium chloride concentrations. Mechanical properties (i.e., breaking force and elasticity) and stability of the encapsulates were then considered to select the most reliable encapsulating formulation to conduct the corresponding alcoholic fermentations. Yeast cells were then encapsulated using 3% (w/v) alginate and 0.1 M calcium chloride as a reticulating agent. Fourteen-day fermentations with this encapsulating formulation involved a Pilsen malt-based wort and four S. cerevisiae strains, three commercially available and one locally isolated. The obtained beer was aged in an amber glass container for two weeks at 4 °C. The color, turbidity, taste, and flavor profile were measured and compared to similar commercially available products. Cell growth was monitored concurrently with fermentation, and the concentrations of ethanol, sugars, and organic acids in the samples were determined via high-performance liquid chromatography (HPLC). It was observed that encapsulation caused significant differences in the sensory profile between strains, as evidenced by marked changes in the astringency, geraniol, and capric acid aroma production. Three repeated batch experiments under the same conditions revealed that cell viability and mechanical properties decreased substantially, which might limit the reusability of encapsulates. In terms of ethanol production and substrate consumption, it was also observed that encapsulation improved the performance of the locally isolated strain.

Microfluidic separators play a pivotal role in the biomedical and chemical industries by enabling precise fluid manipulations. Traditional fabrication of these devices typically requires costly cleanroom facilities, which limits their broader application. This study introduces a novel microfluidic device that leverages the passive Zweifach–Fung principle to overcome these financial barriers. Through Lagrangian computational simulations, we optimized an eleven-channel Zweifach–Fung configuration that achieved a perfect 100% recall rate for particles following a specified normal distribution. Experimental evaluations determined 2 mL/h as the optimal total flow rate (TFR), under which the device showcased exceptional performance enhancements in precision and recall for micrometer-sized particles, achieving an overall accuracy of 94% ± 3%. Fabricated using a cost-effective, non-cleanroom method, this approach represents a significant shift from conventional practices, dramatically reducing production costs while maintaining high operational efficacy. The cost of each chip is less than USD 0.90 cents and the manufacturing process takes only 15 min. The development of this device not only makes microfluidic technology more accessible but also sets a new standard for future advancements in the field.

Magnetoliposomes represent a transformative advancement in nanomedicine by integrating magnetic nanoparticles with liposomal structures, creating multifunctional delivery platforms that overcome key limitations of conventional drug carriers. These hybrid systems enable precision targeting through external magnetic fields, controlled release via magnetic hyperthermia, and real-time theranostic capabilities, offering unprecedented spatiotemporal control over therapeutic administration. This manuscript focused primarily on studies from 2023-2025 however, a few select older references were included to provide background and context.This review examines the fundamental design principles of Magnetoliposomes, including bilayer composition, nanoparticle integration strategies, and physicochemical properties governing their biological performance. We comprehensively assess synthesis methodologies - from traditional thin-film hydration to advanced microfluidic approaches - highlighting their impact on colloidal stability, drug encapsulation, and scaling potential. Characterization techniques essential for quality control and regulatory approval are systematically reviewed, followed by applications across oncology, gene delivery, neurology, and infectious disease treatment, supported by recent experimental evidence. While magnetoliposomes show remarkable therapeutic versatility, their clinical translation requires addressing biocompatibility concerns, manufacturing scalability, and regulatory hurdles. Integration with artificial intelligence, organ-on-chip technologies, and personalized medicine approaches will likely accelerate their development toward clinical reality, potentially revolutionizing treatment paradigms for complex diseases through tailored therapeutic interventions.

This work studied the feasibility of using a novel microreactor based on torus geometry to carry out a sample pretreatment before its analysis by graphite furnace atomic absorption. The miniaturized retention of total arsenic was performed on the surface of a magnetic sorbent material consisting of 6 mg of magnetite (Fe₃O₄) confined in a very small space inside (20.1 µL) a polyacrylate device filling an internal lumen (inside space). Using this geometric design, a simulation theoretical study demonstrated a notable improvement in the analyte adsorption process on the solid extractant surface. Compared to single-layer geometries, the torus microreactor geometry brought on flow turbulence within the liquid along the curvatures inside the device channels, improving the efficiency of analyte–extractant contact and therefore leading to a high preconcentration factor. According to this design, the magnetic solid phase was held internally as a surface bed with the use of an 8 mm-diameter cylindric neodymium magnet, allowing the pass of a fixed volume of an arsenic aqueous standard solution. A preconcentration factor of up to 60 was found to reduce the typical “characteristic mass” (as sensitivity parameter) determined by direct measurement from 53.66 pg to 0.88 pg, showing an essential improvement in the arsenic signal sensitivity by absorption atomic spectrometry. This methodology emulates a miniaturized micro-solid-phase extraction system for flow-through water pretreatment samples in chemical analysis before coupling to techniques that employ reduced sample volumes, such as graphite furnace atomic absorption spectroscopy.

In vitro tissue models are crucial for regenerative medicine, drug discovery, and the reduction of animal testing. 3D bioprinting, particularly when utilizing magnetic manipulation of cell spheroids, provides precise control over tissue architecture. However, existing mathematical models lack the precision to capture the interplay between biological dynamics and magnetic forces during spheroid fusion. This study developed and validated a novel mathematical model that simulates magnetically assisted spheroid fusion, taking into account cell migration, adhesion, and the effects of external magnetic fields. The model integrates principles of cell mechanics, fluid dynamics, and magnetostatics, implemented in COMSOL Multiphysics. Experimental validation used HeLa cell spheroids bioprinted with superparamagnetic iron oxide nanoparticles (SPIONs). Spheroid fusion was monitored with and without an external magnetic field using confocal microscopy. Rigorous statistical analysis (MAE, RMSE, MAPE, R², Chi-Square, Bland-Altman, and variance-weighted metrics) was used to evaluate model performance. The model accurately predicted accelerated fusion under magnetic manipulation, reducing fusion time from approximately 7 days (without field) to 2 days. High R² values (> 0.99 for two-spheroid fusion and > 0.97 for multi-spheroid systems) and narrow confidence intervals demonstrated strong agreement between the simulation and the experiment. Increased system complexity introduced slightly higher error variability, but the model maintained robust predictive capabilities. Spheroid disassembly was observed in the four-spheroid case, highlighting the complex interplay of magnetic forces and cellular reorganization. This validated, high-precision model represents a significant advancement in tissue engineering, providing a powerful tool for optimizing bioprinting protocols, designing complex tissue constructs, and advancing in vitro model development. This breakthrough has implications for regenerative medicine and drug discovery while also highlighting the importance of addressing nanoparticle safety concerns.