Extreme environmental conditions, such as abiotic stresses (drought, salinity, heat, chilling and intense light), offer great opportunities to study how different microorganisms and plant nutrition can influence plant growth and development. The intervention of biological agents such as plant growth-promoting rhizobacteria (PGPRs) coupled with proper plant nutrition can improve the agricultural importance of different plant species. Brassicaceae (Cruciferae) belongs to the monophyletic taxon and consists of around 338 genera and 3709 species worldwide. Brassicaceae is composed of several important species of economical, ornamental and food crops (vegetables, cooking oils, forage, condiments and industrial species). Sustainable production of Brassicas plants has been compromised over the years due to several abiotic stresses and the unbalanced utilization of chemical fertilizers and uncertified chemicals that ultimately affect the environment and human health. This chapter summarized the influence of PGPRs and nutrient management in the Brassicaceae family against abiotic stresses. The use of PGPRs contributed to combating climate-induced change/abiotic factors such as drought, soil and water salinization and heavy metal contamination that limits the general performance of plants. Brassica is widely utilized as an oil and vegetable crop and is harshly affected by abiotic stresses. Therefore, the use of PGPRs along with proper mineral nutrients management is a possible strategy to cope with abiotic stresses by improving biochemical, physiological and growth attributes and the production of brassica in an eco-friendly environment.

This study was designed to investigate the effects of Azospirillum brasilense and Bradyrhizobium sp. co-inoculation coupled with N application on soil N levels and N in plants (total N, nitrate N-NO₃⁻ and ammonium N-NH₄⁺), photosynthetic pigments, cowpea plant biomass and grain yield. An isotopic technique was employed to evaluate ¹⁵N fertilizer recovery and derivation. Field trials involved two inoculations—(i) single Bradyrhizobium sp. and (ii) Bradyrhizobium sp. + A. brasilense co-inoculation—and four N fertilizer rates (0, 20, 40 and 80 kg ha⁻¹). The co-inoculation of Bradyrhizobium sp. + A. brasilense increased cowpea N uptake (an increase from 10 to 14%) and grain yield (an average increase of 8%) compared to the standard inoculation with Bradyrhizobium sp. specifically derived from soil and other sources without affecting ¹⁵N fertilizer recovery. There is no need for the supplementation of N via mineral fertilizers when A. brasilense co-inoculation is performed in a cowpea crop. However, even in the case of an NPK basal fertilization, applied N rates should remain below 20 kg N ha⁻¹ when co-inoculation with Bradyrhizobium sp. and A. brasilense is performed.

Biofortification of cereal crops with zinc and diazotrophic bacteria is a sustainable solution to nutrient deficiency and hidden hunger. The inoculation of staple grain crops such as maize is increased with reducing productivity losses while improving nutrition and use efficiency under climatic extremes and weathered soils of tropical savannah. Therefore, objectives of our study were to evaluate the influence of seed inoculation with diazotrophic bacteria (No inoculation–Control, Azospirillum brasilense, Bacillus subtilis, and Pseudomonas fluorescens) together with residual effect of soil Zn (absence and presence) on growth, yield, Zn nutrition, Zn use efficiencies, and intake of maize in 2019 and 2020 cropping seasons. The inoculation of B. subtilis increased hundred grain mass and yield (14.5 and 17%), while P. fluorescens under residual Zn fertilization has improved shoot and grain Zn concentration in shoot (29.5 and 30.5%). and grain (25.5 and 26.2%), while improving Zn accumulation in shoot (33.8 and 35%) and grain (37.2 and 42%) of maize. The estimated Zn intake in maize was also increased with A. brasilense inoculation and residual Zn application. The Zn use efficiencies including Zn use efficiency, agro-physiological, and utilization efficiency was increased with B. subtilis, while applied Zn recovery was increased with A. brasilense inoculations under residual Zn fertilization. Zinc use efficiency was increased by 93.3 and 397% with inoculation of B. subtilis regardless of Zn application. Therefore, inoculation with B. subtilis and P. fluorescens along residual Zn fertilization is considered the most effective and sustainable strategy for agronomic biofortification of maize under harsh tropical conditions of Brazil.

In Brazil, several silicic rocks can be used as powder-based K sources, which can reduce production costs in agriculture. The optimized supply of K not only increases yield but also contributes to soil fertility preservation and long-term sustainability by curtailing nutrient losses and reducing the risk of nutrient imbalances. Therefore, this study aimed to investigate the effects of K application timing, source, and doses on nodulation, productive components, and productivity of soybeans in a not-tillage system in the Savannah. The experiment was carried out in the field, for two years, in an Oxisol, with a clayey texture. The experimental design was in random blocks, in a 2 × 4 × 3 factorial scheme, as follows: two application timings (early and sowing), four K₂O rates (0, 40, 80, and 120 kg ha⁻¹), and three sources (KCl, Potasil, and Ekosil), with four replicates. Potassium fertilizer was broadcasted on the soil without incorporation into the soil. Due to the interactions between doses and K₂O sources, there was a linear adjustment for KCl sources, the higher the dose, the lower the nodule mass. Also, for nodule mass, the interaction between dose and application time was significant for the early application of the Ecosil and Potasil sources for 80 kg ha⁻¹. The highest estimated soybean grain productivity was 3262 kg ha⁻¹ with 78 kg ha⁻¹ of K₂O, being the most suitable for growing soybeans under a no-tillage system.