Many living organisms have DNA in their cells that is responsible for their biological features. DNA is an organic molecule of two complementary strands of four different nucleotides wound up in a double helix. These nucleotides are adenine (A), thymine (T), guanine (G), and cytosine (C). Genes are DNA sequences containing the information to synthesize proteins. The genes of higher eukaryotic organisms contain coding sequences, known as exons and non-coding sequences, known as introns, which are removed on splice sites after the DNA is transcribed into RNA. Genome annotation is the process of identifying the location of coding regions and determining their function. This process is fundamental for understanding gene structure; however, it is time-consuming and expensive when done by biochemical methods. With technological advances, splice site detection can be done computationally. Although various software tools have been developed to predict splice sites, they need to improve accuracy and reduce false-positive rates. The main goal of this research was to generate Deep Splicer, a deep learning model to identify splice sites in the genomes of humans and other species. This model has good performance metrics and a lower false-positive rate than the currently existing tools. Deep Splicer achieved an accuracy between 93.55% and 99.66% on the genetic sequences of different organisms, while Splice2Deep, another splice site detection tool, had an accuracy between 90.52% and 98.08%. Splice2Deep surpassed Deep Splicer on the accuracy obtained after evaluating C. elegans genomic sequences (97.88% vs. 93.62%) and A. thaliana (95.40% vs. 94.93%); however, Deep Splicer’s accuracy was better for H. sapiens (98.94% vs. 97.15%) and D. melanogaster (97.14% vs. 92.30%). The rate of false positives was 0.11% for human genetic sequences and 0.25% for other species’ genetic sequences. Another splice prediction tool, Splice Finder, had between 1% and 3% of false positives for human sequences, while other species’ sequences had around 4% and 10%.

The world is facing the COVID-19 pandemic, leading to an unprecedented change in the lifestyle routines of millions. Beyond the general physical health, financial, and social repercussions of the pandemic, the adopted mitigation measures also present significant challenges in the populations mental health and health programs. It is complex for public organizations to measure the populations mental health in order to incorporate it into their own decision-making process. Traditional survey methods are time-consuming, expensive, and fail to provide the continuous information needed to respond to the rapidly evolving effects of governmental policies on the populations mental health. A significant portion of the population has turned to social media to express the details of their daily life, rendering this public data a rich field for understanding emotional and mental well-being. This study aims to track and measure the sentiment changes of the Mexican population in response to the COVID-19 pandemic. To this end, we analyzed 760,064,879 public domain tweets collected from a public access repository to examine the collective shifts in the general mood about the pandemic evolution, news cycles, and governmental policies using open sentiment analysis tools. Sentiment analysis polarity scores, which oscillate around -0.15, show a weekly seasonality according to Twitters usage and a consistently negative outlook from the population. It also remarks on the increased controversy after the governmental decision to terminate the lockdown and the celebrated holidays, which encouraged the people to incur social gatherings. These findings expose the adverse emotional effects of the ongoing pandemic while showing an increase in social media usage rates of 2.38 times, which users employ as a coping mechanism to mitigate the feelings of isolation related to long-term social distancing. The findings have important implications in the mental health infrastructure for ongoing mitigation efforts and feedback on the perception of policies and other measures. The overall trend of the sentiment polarity is 0.0001110643.

The world has been facing the COVID-19 pandemic, which has come with an unprecedented impact on general physical health and financial and social repercussions. The adopted mitigation measures also present significant challenges to the population’s mental health and health-related programs. It is complex for public organizations to measure the population’s mental health to incorporate its feedback into their decision-making process. A significant portion of the population has turned to social media to express the details of their daily life, making these public data a rich field for understanding emotional and mental well-being. To this end, by using open sentiment analysis tools, we analyzed 760,064,879 public domain tweets collected from a public access repository to examine the collective shifts in the general mood about the pandemic evolution, news cycles, and governmental policies. Several modern language models were evaluated and compared using intrinsic and extrinsic tasks, that is, the sentiment analysis evaluation of public domain tweets related to the COVID-19 pandemic in Mexico. This study provides a fair evaluation of state-of-the-art language models, such as BERT and VADER, showcasing their metrics and comparing their performance against a real-world task. Results show the importance of selecting the correct language model for large projects such as this one, for there is a need to balance costs with the model’s performance.

Risk assessment is relevant in any workplace, however, there is a degree of unpredictability when dealing with flammable or hazardous materials so that detection of fire accidents by itself may not be enough. An example of this is the impingement of jet fires, where the heat fluxes of the flame could reach nearby equipment and dramatically increase the probability of a domino effect with catastrophic results. Because of this, the characterization of such fire accidents is important from a risk management point of view. One such characterization would be the segmentation of different radiation zones within the flame, so this paper presents exploratory research regarding several traditional computer vision and Deep Learning segmentation approaches to solving this specific problem. A data set of propane jet fires is used to train and evaluate the different approaches and given the difference in the distribution of the zones and background of the images, different loss functions, that seek to alleviate data imbalance, are also explored. Additionally, different metrics are correlated to a manual ranking performed by experts to make an evaluation that closely resembles the expert’s criteria. The Hausdorff Distance and Adjusted Rand Index were the metrics with the highest correlation and the best results were obtained from the UNet architecture with a Weighted Cross-Entropy Loss. These results can be used in future research to extract more geometric information from the segmentation masks or could even be implemented on other types of fire accidents.