This page has only limited features, please log in for full access.
The study is based on phytochemical profiling and in vitro evaluation of biological effects of phenolic acid derivatives-rich Herniaria fractions, isolated from two rupturewort (Herniaria L.) species, i.e. Herniaria incana Lam. (syn. H. besseri Fisch. ex Hornem) and H. polygama J. Gay (syn. H. odorata). For the first time, the composition of phenolic compounds of these species was extensively evaluated by both LC-HR-QTOF-ESI-MS and Nuclear Magnetic Resonance spectroscopy (NMR). LC-MS analyses of H. polygama revealed 72 tentatively identified compounds, while H. incana – 63. Only 8% of the metabolites reported in this work have been previously described for Herniaria spp. Most of the identified specialized metabolites were cinnamic and benzoic acid derivatives. Phenolic fraction of H. incana herb contained flavonoids as well. A multi-step chromatographic separation of phenolic fractions from H. polygama yielded three known cinnamic and one benzoic acid derivates, and from H. incana – 4 known flavonoids and one previously undescribed, i.e. rhamnocitrin-3-O-[3-hydroxy-3-methylglutaryl-(1 → 6′′)]-[α-rhamnopyranosyl-(1 → 2′′)]-β-glucopyranoside. Antioxidant properties of the examined fractions (1–50 μg/ml) were assessed in human blood plasma under the conditions of peroxynitrite-induced oxidative stress. Measurements of well-known biomarkers such as 3-nitrotyrosine, protein thiol groups, thiobarbituric acid-reactive substances and the ferric reducing ability of blood plasma revealed the protective effect of Herniaria fractions against oxidative damage to blood plasma components. Furthermore, the examined fractions effectively ameliorated the inflammatory response of the concanavalin A-stimulated human peripheral blood mononuclear cells (PBMCs). Additionally, cellular safety of the fractions was confirmed in PBMCs.
Joanna Kolodziejczyk-Czepas; Solomiia Kozachok; Łukasz Pecio; Svitlana Marchyshyn; Wiesław Oleszek. Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects. Phytochemistry 2021, 190, 112861 .
AMA StyleJoanna Kolodziejczyk-Czepas, Solomiia Kozachok, Łukasz Pecio, Svitlana Marchyshyn, Wiesław Oleszek. Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects. Phytochemistry. 2021; 190 ():112861.
Chicago/Turabian StyleJoanna Kolodziejczyk-Czepas; Solomiia Kozachok; Łukasz Pecio; Svitlana Marchyshyn; Wiesław Oleszek. 2021. "Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects." Phytochemistry 190, no. : 112861.
The bark of Aesculus hippocastanum is an herbal remedy used in conditions connected with vascular insufficiency; however, there is a lack of data concerning its mechanisms of action. The present work is a preliminary investigation into some of the potential directions of the bark activity. The phytochemically (qualitative UHPLC-PDA-MS/MS and quantitative UHPLC-PDA assays) characterized extract and its four main constituents (esculin, fraxin, (‒)-epicatechin and procyanidin A2) were first evaluated in terms of their antioxidant capacity. All analytes demonstrated dose-dependent scavenging potential towards the most common in vivo oxidants, with particularly advantageous capacity of the extract and its flavan-3-ol constituents against peroxynitrite (3.37–13.26 mmol AA/g), hydroxyl radical (5.03–8.91 mmol AA/g) and superoxide radical (3.50–5.50 mmol AA/g). Moreover, even at low concentrations (1–5 µg/mL), they protected components of human plasma against oxidative damage inflicted by peroxynitrite, preventing oxidation of plasma protein thiols and diminishing the tyrosine nitration and lipid peroxidation. High efficiency of the analytes was also demonstrated in preventing the peroxynitrite-induced nitrative changes of fibrinogen (up to 80% inhibition for (‒)-epicatechin at 50 µg/mL), an important protein of coagulation cascade. Additionally, the extract and its constituents had, at most, moderate inhibitory activity towards platelet aggregation induced by ADP and only negligible influence on clotting times. The results show that, among the investigated properties, the antioxidant activity might, to the highest extent, be responsible for the bark efficacy in vascular disorders, thus supporting its application in those conditions; they also indicate the directions for future research that would allow for better understanding of the bark activity.
Aleksandra Owczarek; Joanna Kolodziejczyk-Czepas; Joanna Woźniak-Serwata; Anna Magiera; Natalia Kobiela; Katarzyna Wąsowicz; Monika Olszewska. Potential Activity Mechanisms of Aesculus hippocastanum Bark: Antioxidant Effects in Chemical and Biological In Vitro Models. Antioxidants 2021, 10, 995 .
AMA StyleAleksandra Owczarek, Joanna Kolodziejczyk-Czepas, Joanna Woźniak-Serwata, Anna Magiera, Natalia Kobiela, Katarzyna Wąsowicz, Monika Olszewska. Potential Activity Mechanisms of Aesculus hippocastanum Bark: Antioxidant Effects in Chemical and Biological In Vitro Models. Antioxidants. 2021; 10 (7):995.
Chicago/Turabian StyleAleksandra Owczarek; Joanna Kolodziejczyk-Czepas; Joanna Woźniak-Serwata; Anna Magiera; Natalia Kobiela; Katarzyna Wąsowicz; Monika Olszewska. 2021. "Potential Activity Mechanisms of Aesculus hippocastanum Bark: Antioxidant Effects in Chemical and Biological In Vitro Models." Antioxidants 10, no. 7: 995.
Oxidative post-translational modifications of fibrinogen (a multifunctional blood plasma protein essential for hemostasis) are associated with the pathogenesis of cardiovascular disorders (CVDs). Prunus spinosa flower is a herbal medicine used in an adjuvant treatment of CVDs and rich in polyphenolic antioxidants. In the present study, phytochemically standardized P. spinosa flower extracts, their primary native polyphenols and potential phenolic metabolites were evaluated in vitro for their protective effects on fibrinogen (isolated and in the human plasma matrix) using a panel of complementary methods (SDS-PAGE, western blot, C-ELISA, fluorometry, FRAP, TBARS). The results revealed that the tested analytes at in vivo relevant levels (1–5 µg/mL) considerably reduced the structural changes in the fibrinogen molecule under the oxidative stress conditions induced by peroxynitrite. In particular, they diminished the oxidation and/or nitration of amino acid residues, including tyrosine and tryptophan, as well as the formation of high molecular weight aggregates. The decrease in the levels of 3-nitrotyrosine was about 13.5–33.0% and 58.3–97.1% at 1 µg/mL and 50 µg/mL, respectively. The study indicated that low molecular weight polyphenols were crucial for the protective activity of the extracts toward fibrinogen and other human plasma components. The investigated model compounds effectively protected total plasma proteins and lipids against oxidative damage (by reducing the levels of 3-nitrotyrosine and thiobarbituric acid-reactive substances and normalizing/enhancing the non-enzymatic antioxidant capacity of plasma). The work provides insight into the role of native and metabolized polyphenols as contributory factors to the systemic activity of blackthorn flower extracts within the circulatory system.
Anna Marchelak; Joanna Kolodziejczyk-Czepas; Paulina Wasielewska; Pawel Nowak; Monika Olszewska. The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro. Antioxidants 2021, 10, 581 .
AMA StyleAnna Marchelak, Joanna Kolodziejczyk-Czepas, Paulina Wasielewska, Pawel Nowak, Monika Olszewska. The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro. Antioxidants. 2021; 10 (4):581.
Chicago/Turabian StyleAnna Marchelak; Joanna Kolodziejczyk-Czepas; Paulina Wasielewska; Pawel Nowak; Monika Olszewska. 2021. "The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro." Antioxidants 10, no. 4: 581.
Introduction Background: Due to an imbalanced redox status, cancer cells generate intrinsically higher levels of reactive oxygen species (ROS), compared to normal cells. Targeting ROS is an important therapeutic strategy for cancer as exemplified by cancer drugs, which induce ROS-dependent synergistic cytotoxicity in gastric cancer cells. The present study was designed to assess the level of selected oxidative stress biomarkers in blood plasma, derived from gastric cancer patients. Material and methods The study included the assessment of the oxidative/nitrative biomarkers in blood plasma isolated form 51gastric (adenocarcinoma) cancer patients, compared to a control group of 32 healthy volunteers. Oxidative stress was evaluated using a panel of biomarkers such as plasma protein thiol groups and 3-nitrotyrosine levels as well as indicators of plasma lipid peroxidation, i.e. lipid hydroperoxides (LOOH) and thiobarbituric acid-reactive substances (TBARS). Additionally, the total antioxidant capacity of blood plasma (non-enzymatic capacity of blood plasma, NEAC) was also estimated. Results Our results showed that patients with gastric cancer had changed levels of thiol groups (a decrease, p Conclusions The presented study indicates on a considerable oxidative/nitrative stress in gastric cancer patients. Our pilot study shows that not a single marker, but a biomarkers panel may be a more reliable representation of oxidative stress in patients with gastric cancer.
Jarosław Andrzej Jakubik; Joanna Kołodziejczyk-Czepas; Magdalena Kędzierska; Michał Kaczmarek; Paweł Nowak; Piotr Potemski; Arkadiusz Jeziorski. A preliminary evaluation of oxidative stress in patients with gastric cancer before chemotherapy. Archives of Medical Science 2021, 1 .
AMA StyleJarosław Andrzej Jakubik, Joanna Kołodziejczyk-Czepas, Magdalena Kędzierska, Michał Kaczmarek, Paweł Nowak, Piotr Potemski, Arkadiusz Jeziorski. A preliminary evaluation of oxidative stress in patients with gastric cancer before chemotherapy. Archives of Medical Science. 2021; ():1.
Chicago/Turabian StyleJarosław Andrzej Jakubik; Joanna Kołodziejczyk-Czepas; Magdalena Kędzierska; Michał Kaczmarek; Paweł Nowak; Piotr Potemski; Arkadiusz Jeziorski. 2021. "A preliminary evaluation of oxidative stress in patients with gastric cancer before chemotherapy." Archives of Medical Science , no. : 1.
The Pulmonaria species (lungwort) are edible plants and traditional remedies for different disorders of the respiratory system. Our work covers a comparative study on biological actions in human blood plasma and cyclooxygenase-2 (COX-2) -inhibitory properties of plant extracts (i.e., phenolic-rich fractions) originated from aerial parts of P. obscura Dumort. and P. officinalis L. Phytochemical profiling demonstrated the abundance of phenolic acids and their derivatives (over 80% of the isolated fractions). Danshensu conjugates with caffeic acid, i.e., rosmarinic, lithospermic, salvianolic, monardic, shimobashiric and yunnaneic acids were identified as predominant components. The examined extracts (1–100 µg/mL) partly prevented harmful effects of the peroxynitrite-induced oxidative stress in blood plasma (decreased oxidative damage to blood plasma components and improved its non-enzymatic antioxidant capacity). The cellular safety of the extracts was confirmed in experimental models of blood platelets and peripheral blood mononuclear cells. COX-2 inhibitor screening evidently suggested a stronger activity of P. officinalis (IC50 of 13.28 and 7.24 µg/mL, in reaction with synthetic chromogen and physiological substrate (arachidonic acid), respectively). In silico studies on interactions of main components of the Pulmonaria extracts with the COX-2 demonstrated the abilities of ten compounds to bind with the enzyme, including rosmarinic acid, menisdaurin, globoidnan A and salvianolic acid H.
Justyna Krzyżanowska-Kowalczyk; Mariusz Kowalczyk; Michał Ponczek; Łukasz Pecio; Paweł Nowak; Joanna Kolodziejczyk-Czepas. Pulmonaria obscura and Pulmonaria officinalis Extracts as Mitigators of Peroxynitrite-Induced Oxidative Stress and Cyclooxygenase-2 Inhibitors–In Vitro and In Silico Studies. Molecules 2021, 26, 631 .
AMA StyleJustyna Krzyżanowska-Kowalczyk, Mariusz Kowalczyk, Michał Ponczek, Łukasz Pecio, Paweł Nowak, Joanna Kolodziejczyk-Czepas. Pulmonaria obscura and Pulmonaria officinalis Extracts as Mitigators of Peroxynitrite-Induced Oxidative Stress and Cyclooxygenase-2 Inhibitors–In Vitro and In Silico Studies. Molecules. 2021; 26 (3):631.
Chicago/Turabian StyleJustyna Krzyżanowska-Kowalczyk; Mariusz Kowalczyk; Michał Ponczek; Łukasz Pecio; Paweł Nowak; Joanna Kolodziejczyk-Czepas. 2021. "Pulmonaria obscura and Pulmonaria officinalis Extracts as Mitigators of Peroxynitrite-Induced Oxidative Stress and Cyclooxygenase-2 Inhibitors–In Vitro and In Silico Studies." Molecules 26, no. 3: 631.
A wide range of traditional medicine applications of Uncaria tomentosa (Willd. ex Schult.) DC., commonly known as ‘vilcacora’ or ‘cat's claw’, includes blood purification, its anticoagulant properties and its use in haemorrhage therapy. Our work is devoted to the effects of ethanol and aqueous extracts (1–50 μg/ml) from U. tomentosa leaves and bark on the haemostatic system. The study is based on two main questions: Can these extracts influence the coagulation cascade of blood plasma or the activation of blood platelets? Do they feature any anticoagulant properties? Blood platelet aggregation was measured in human platelet-rich plasma; the anticoagulant tests were based on the thrombin, prothrombin and the activated partial thromboplastin time. For the thrombin (TH)-inhibitory activity evaluation, the chromogenic substrate S-2238 and fibrinogen, i.e. physiological substrate for this enzyme, were used. In silico studies included the interactions of TH and the main components of the extracts. The examined extracts demonstrated slight antiplatelet activity. The thrombin time was slightly prolonged. The most efficient TH inhibitor was the ethanolic fraction from leaves (IC50 = 5.86 and 12.48 μg/ml, for the amidolytic and proteolytic assay, respectively). The plant ingredients interacted with TH within and outside the active site, dependently on the compound. The higher binding affinity was found for procyanidins B2 and C1. The examined extracts demonstrated slight antiplatelet effects; however, they may be promising candidates for the natural inhibitors of TH, which is critical for the formation of fibrin clot.
Joanna Kolodziejczyk-Czepas; Michał Ponczek; Magdalena Sady-Janczak; Radosław Pilarski; Bożena Bukowska. Extracts from Uncaria tomentosa as antiplatelet agents and thrombin inhibitors – The in vitro and in silico study. Journal of Ethnopharmacology 2020, 267, 113494 .
AMA StyleJoanna Kolodziejczyk-Czepas, Michał Ponczek, Magdalena Sady-Janczak, Radosław Pilarski, Bożena Bukowska. Extracts from Uncaria tomentosa as antiplatelet agents and thrombin inhibitors – The in vitro and in silico study. Journal of Ethnopharmacology. 2020; 267 ():113494.
Chicago/Turabian StyleJoanna Kolodziejczyk-Czepas; Michał Ponczek; Magdalena Sady-Janczak; Radosław Pilarski; Bożena Bukowska. 2020. "Extracts from Uncaria tomentosa as antiplatelet agents and thrombin inhibitors – The in vitro and in silico study." Journal of Ethnopharmacology 267, no. : 113494.
Cotoneaster plants are sources of traditional medicines and dietary products, with health benefits resulting from their phenolic contents and antioxidant activity. In this work, active markers of the leaves of C. bullatus and C. zabelii were characterized and evaluated in an integrated phytochemical and biological activity study. Based on UHPLC-PDA-ESI-MS3 analysis, twelve analytes were preselected from the constituents of the hydromethanolic leaf extracts, and two of them—caffeoylmalic acid and quercetin 3--O-β-d-(2″--O-β-d-xylopyranosyl)galactopyranoside (QPH)—were isolated for full identification (NMR spectroscopy: 1H, 13C, COSY, HMBC, HMQC). All selected phenolics contributed to the antioxidant activity of the extracts, which was demonstrated in chemical in vitro tests (DPPH, FRAP, and TBARS) and in a biological model of human plasma exposed to oxidative/nitrative stress induced by peroxynitrite. This contribution was partly due to the synergy between individual polyphenols, evidenced by an isobolographic analysis of the interactions of (–)-epicatechin, chlorogenic acid, and QPH as representatives of three classes of Cotoneaster polyphenols. All twelve markers, including also neochlorogenic acid, cryptochlorogenic acid, procyanidin B2, procyanidin C1, rutin, hyperoside, isoquercitrin, and quercitrin, were thus applied as calibration standards, and a fast, accurate, reproducible, and fully validated RP-HPLC-PDA method for quality control and standardization of the target extracts was proposed.
Agnieszka Kicel; Aleksandra Owczarek; Paulina Kapusta; Joanna Kolodziejczyk-Czepas; Monika A. Olszewska. Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control. Antioxidants 2020, 9, 69 .
AMA StyleAgnieszka Kicel, Aleksandra Owczarek, Paulina Kapusta, Joanna Kolodziejczyk-Czepas, Monika A. Olszewska. Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control. Antioxidants. 2020; 9 (1):69.
Chicago/Turabian StyleAgnieszka Kicel; Aleksandra Owczarek; Paulina Kapusta; Joanna Kolodziejczyk-Czepas; Monika A. Olszewska. 2020. "Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control." Antioxidants 9, no. 1: 69.
Rhaponticin (3,3′,5-trihydroxy-4′-methoxystilbene 3-O-β-d-glucoside; synonym–rhapontin), is a stilbene compound, mainly found in various species of rhubarb (Rheum L.). The aglycone, rhapontigenin, is thought to be the biologically active form of rhaponticin. As a component of rhubarb, rhaponticin may be present in the human diet both in foods and dietary supplements. Although promising reports have been published on the various activities of rhaponticin (see: antioxidant, estrogenic, antithrombotic, anticancer and anti-inflammatory actions), no comprehensive review exists of its anti-inflammatory properties. Therefore, the aim of the present work is to summarize the existing evidence of the anti-inflammatory properties of rhaponticin and provide a prospective insight into research on this stilbenoid.
Joanna Kolodziejczyk-Czepas; Jan Czepas. Rhaponticin as an anti-inflammatory component of rhubarb: a minireview of the current state of the art and prospects for future research. Phytochemistry Reviews 2019, 18, 1375 -1386.
AMA StyleJoanna Kolodziejczyk-Czepas, Jan Czepas. Rhaponticin as an anti-inflammatory component of rhubarb: a minireview of the current state of the art and prospects for future research. Phytochemistry Reviews. 2019; 18 (5):1375-1386.
Chicago/Turabian StyleJoanna Kolodziejczyk-Czepas; Jan Czepas. 2019. "Rhaponticin as an anti-inflammatory component of rhubarb: a minireview of the current state of the art and prospects for future research." Phytochemistry Reviews 18, no. 5: 1375-1386.
Sorbus domestica leaves are a traditionally used herbal medicine recommended for the treatment of oxidative stress-related diseases. Dry leaf extracts (standardized by LC-MS/MS and LC-PDA) and nine model activity markers (polyphenols), were tested in scavenging assays towards six in vivo-relevant oxidants (O2•−, OH•, NO•, H2O2, ONOO−, HClO). Ascorbic acid (AA) and Trolox (TX) were used as positive standards. The most active extracts were the diethyl ether and ethyl acetate fractions with activities in the range of 3.61–20.03 µmol AA equivalents/mg, depending on the assay. Among the model compounds, flavonoids were especially effective in OH• scavenging, while flavan-3-ols were superior in O2•− quenching. The most active constituents were quercetin, (−)-epicatechin, procyanidins B2 and C1 (3.94–24.16 µmol AA/mg), but considering their content in the extracts, isoquercitrin, (−)-epicatechin and chlorogenic acid were indicated as having the greatest influence on extract activity. The analysis of the synergistic effects between those three compounds in an O2•− scavenging assay demonstrated that the combination of chlorogenic acid and isoquercitrin exerts the greatest influence. The results indicate that the extracts possess a strong and broad spectrum of antioxidant capacity and that their complex composition plays a key role, with various constituents acting complementarily and synergistically.
Magdalena Rutkowska; Monika Anna Olszewska; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Aleksandra Owczarek. Sorbus domestica Leaf Extracts and Their Activity Markers: Antioxidant Potential and Synergy Effects in Scavenging Assays of Multiple Oxidants. Molecules 2019, 24, 2289 .
AMA StyleMagdalena Rutkowska, Monika Anna Olszewska, Joanna Kolodziejczyk-Czepas, Pawel Nowak, Aleksandra Owczarek. Sorbus domestica Leaf Extracts and Their Activity Markers: Antioxidant Potential and Synergy Effects in Scavenging Assays of Multiple Oxidants. Molecules. 2019; 24 (12):2289.
Chicago/Turabian StyleMagdalena Rutkowska; Monika Anna Olszewska; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Aleksandra Owczarek. 2019. "Sorbus domestica Leaf Extracts and Their Activity Markers: Antioxidant Potential and Synergy Effects in Scavenging Assays of Multiple Oxidants." Molecules 24, no. 12: 2289.
Anna Marchelak; Aleksandra Owczarek; Magdalena Rutkowska; Piotr Michel; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Monika Anna Olszewska. New insights into antioxidant activity of Prunus spinosa flowers: Extracts, model polyphenols and their phenolic metabolites in plasma towards multiple in vivo-relevant oxidants. Phytochemistry Letters 2019, 30, 288 -295.
AMA StyleAnna Marchelak, Aleksandra Owczarek, Magdalena Rutkowska, Piotr Michel, Joanna Kolodziejczyk-Czepas, Pawel Nowak, Monika Anna Olszewska. New insights into antioxidant activity of Prunus spinosa flowers: Extracts, model polyphenols and their phenolic metabolites in plasma towards multiple in vivo-relevant oxidants. Phytochemistry Letters. 2019; 30 ():288-295.
Chicago/Turabian StyleAnna Marchelak; Aleksandra Owczarek; Magdalena Rutkowska; Piotr Michel; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Monika Anna Olszewska. 2019. "New insights into antioxidant activity of Prunus spinosa flowers: Extracts, model polyphenols and their phenolic metabolites in plasma towards multiple in vivo-relevant oxidants." Phytochemistry Letters 30, no. : 288-295.
This work is aimed at establishing analytical and bioactivity markers for the quality control of polyphenol-rich extracts of Sorbus domestica leaves – an antioxidant and anti-inflammatory agent. The potential marker analytes, representative of the main groups of S. domestica polyphenols, were pre-selected by LC–MS/MS analysis of the methanol-water (7:3, v/v) leaf extract (MED). For structural identification, two of them (rare flavonoids) were isolated from MED by preparative HPLC and analysed by 1D and 2D NMR (COSY, HMBC, HMQC) spectroscopy. The in vitro tests revealed that all analytes increase the non-enzymatic antioxidant capacity of plasma, effectively protect human plasma components against oxidative/nitrative damage induced by peroxynitrite (reduce the levels of thiobarbituric acid-reactive substances and 3-nitrotyrosine), and inhibit lipoxygenase and hyaluronidase. Considering the activity parameters and concentration levels, the highest contribution (70–80%) to the activity of the total phenolic fraction of MED was attributed to flavanols represented by (–)-epicatechin, procyanidin B2 and procyanidin C1. However, taking into account the observed synergistic effects, flavonoid and phenolic acid fractions (represented by chlorogenic acid, quercitrin, rutin, quercetin and its 3-O-(2′'-O-β-d-glucopyranosyl)-α-l-rhamnopyranoside and 3-O-(2′'-O-β-d-xylopyranosyl)-α-l-rhamnopyranoside) had also a substantial impact. All nine markers were thus applied as calibration standards, and a simple, accurate, reproducible and fully validated RP-HPLC-PDA method for the standardisation of the target extracts was proposed.
Magdalena Rutkowska; Aleksandra Owczarek; Joanna Kolodziejczyk-Czepas; Piotr Michel; Dorota Gabriela Piotrowska; Paulina Kapusta; Pawel Nowak; Monika Olszewska. Identification of bioactivity markers of Sorbus domestica leaves in chromatographic, spectroscopic and biological capacity tests: Application for the quality control. Phytochemistry Letters 2019, 30, 278 -287.
AMA StyleMagdalena Rutkowska, Aleksandra Owczarek, Joanna Kolodziejczyk-Czepas, Piotr Michel, Dorota Gabriela Piotrowska, Paulina Kapusta, Pawel Nowak, Monika Olszewska. Identification of bioactivity markers of Sorbus domestica leaves in chromatographic, spectroscopic and biological capacity tests: Application for the quality control. Phytochemistry Letters. 2019; 30 ():278-287.
Chicago/Turabian StyleMagdalena Rutkowska; Aleksandra Owczarek; Joanna Kolodziejczyk-Czepas; Piotr Michel; Dorota Gabriela Piotrowska; Paulina Kapusta; Pawel Nowak; Monika Olszewska. 2019. "Identification of bioactivity markers of Sorbus domestica leaves in chromatographic, spectroscopic and biological capacity tests: Application for the quality control." Phytochemistry Letters 30, no. : 278-287.
Polyphenol-rich plant extracts might alleviate the negative impact of oxidative stress and inflammation, but careful phytochemical standardisation and evaluation of various mechanisms are required to fully understand their effects. In this context, flower extracts of Sorbus aucuparia L.—a traditional medicinal plant—were investigated in the present work. The LC-MS/MS profiling of the extracts, obtained by fractionated extraction, led to the identification of 66 constituents, mostly flavonols (quercetin and sexangularetin glycosides with dominating isoquercitrin), pseudodepsides of quinic and shikimic acids (prevailing isomers of chlorogenic acid and cynarin), and flavanols (catechins and proanthocyanidins). Minor extract components of possible chemotaxonomic value were flavalignans (cinchonain I isomers) and phenylamides (spermidine derivatives). As assessed by HPLC-PDA and UV-spectrophotometric studies, the extracts were polyphenol-abundant, with the contents up to 597.6 mg/g dry weight (dw), 333.9 mg/g dw, 382.0 mg/g dw, and 169.0 mg/g dw of total phenolics, flavonoids, proanthocyanidins, and caffeoylquinic acids, respectively. Their biological in vitro effects were phenolic-dependent and the strongest for diethyl ether, ethyl acetate, and n-butanol fractions of the methanol-water (7 : 3, v/v) extract. The extracts showed significant, concentration-dependent ability to scavenge in vivo-relevant radical/oxidant agents (O2∙−, OH∙, H2O2, ONOO–, NO∙, and HClO) with the strongest effects towards OH∙, ONOO–, HClO, and O2∙− (compared to ascorbic acid). Moreover, the extracts efficiently inhibited lipoxygenase and hyaluronidase (compared to indomethacin) but were inactive towards xanthine oxidase. At in vivo-relevant levels (1-5 μg/mL), they also effectively protected human plasma components (proteins and lipids) against ONOO–-induced oxidative damage (reduced the levels of 3-nitrotyrosine, lipid hydroperoxides, and thiobarbituric acid-reactive substances) and normalised/enhanced the total nonenzymatic antioxidant capacity of plasma. In cytotoxicity tests, the extracts did not affect the viability of human PBMCs and might be regarded as safe. The results support the application of the extracts in the treatment of oxidative stress-related pathologies cross-linked with inflammatory changes.
Monika A. Olszewska; Joanna Kolodziejczyk-Czepas; Magdalena Rutkowska; Anna Magiera; Piotr Michel; Marcin W. Rejman; Pawel Nowak; Aleksandra Owczarek. The Effect of Standardised Flower Extracts of Sorbus aucuparia L. on Proinflammatory Enzymes, Multiple Oxidants, and Oxidative/Nitrative Damage of Human Plasma Components In Vitro. Oxidative Medicine and Cellular Longevity 2019, 2019, 1 -18.
AMA StyleMonika A. Olszewska, Joanna Kolodziejczyk-Czepas, Magdalena Rutkowska, Anna Magiera, Piotr Michel, Marcin W. Rejman, Pawel Nowak, Aleksandra Owczarek. The Effect of Standardised Flower Extracts of Sorbus aucuparia L. on Proinflammatory Enzymes, Multiple Oxidants, and Oxidative/Nitrative Damage of Human Plasma Components In Vitro. Oxidative Medicine and Cellular Longevity. 2019; 2019 ():1-18.
Chicago/Turabian StyleMonika A. Olszewska; Joanna Kolodziejczyk-Czepas; Magdalena Rutkowska; Anna Magiera; Piotr Michel; Marcin W. Rejman; Pawel Nowak; Aleksandra Owczarek. 2019. "The Effect of Standardised Flower Extracts of Sorbus aucuparia L. on Proinflammatory Enzymes, Multiple Oxidants, and Oxidative/Nitrative Damage of Human Plasma Components In Vitro." Oxidative Medicine and Cellular Longevity 2019, no. : 1-18.
The work presents the results of an investigation into the molecular background of the activity of Cotoneaster fruits, providing a detailed description of their phytochemical composition and some of the mechanisms of their anti-inflammatory and antioxidant effects. GS-FID-MS and UHPLC-PDA-ESI-MS3 methods were applied to identify the potentially health-beneficial constituents of lipophilic and hydrophilic fractions, leading to the identification of fourteen unsaturated fatty acids (with dominant linoleic acid, 375.4–1690.2 mg/100 g dw), three phytosterols (with dominant β-sitosterol, 132.2–463.3 mg/100 g), two triterpenoid acids (10.9–54.5 mg/100 g), and twenty-six polyphenols (26.0–43.5 mg GAE/g dw). The most promising polyphenolic fractions exhibited dose-dependent anti-inflammatory activity in in vitro tests of lipoxygenase (IC50 in the range of 7.7–24.9 μg/U) and hyaluronidase (IC50 in the range of 16.4–29.3 μg/U) inhibition. They were also demonstrated to be a source of effective antioxidants, both in in vitro chemical tests (DPPH, FRAP, and TBARS) and in a biological model, in which at in vivo-relevant levels (1–5 μg/mL) they normalized/enhanced the nonenzymatic antioxidant capacity of human plasma and efficiently protected protein and lipid components of plasma against peroxynitrite-induced oxidative/nitrative damage. Moreover, the investigated extracts did not exhibit cytotoxicity towards human PMBCs. Among the nine Cotoneaster species tested, C. hjelmqvistii, C. zabelii, C. splendens, and C. bullatus possess the highest bioactive potential and might be recommended as dietary and functional food products.
Agnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Magdalena Rutkowska; Anna Wajs-Bonikowska; Sebastian Granica; Pawel Nowak; Monika A. Olszewska. Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro. Oxidative Medicine and Cellular Longevity 2018, 2018, 1 -16.
AMA StyleAgnieszka Kicel, Joanna Kolodziejczyk-Czepas, Aleksandra Owczarek, Magdalena Rutkowska, Anna Wajs-Bonikowska, Sebastian Granica, Pawel Nowak, Monika A. Olszewska. Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro. Oxidative Medicine and Cellular Longevity. 2018; 2018 ():1-16.
Chicago/Turabian StyleAgnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Magdalena Rutkowska; Anna Wajs-Bonikowska; Sebastian Granica; Pawel Nowak; Monika A. Olszewska. 2018. "Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro." Oxidative Medicine and Cellular Longevity 2018, no. : 1-16.
The present study investigated the phenolic profile and biological activity of dry extracts from leaves of C. bullatus, C. zabelii and C. integerrimus—traditional medicinal and dietary plants—and evaluated their potential in adjunctive therapy of cardiovascular diseases. Complementary UHPLC-PDA-ESI-MS3, HPLC-PDA-fingerprint, Folin-Ciocalteu, and n-butanol/HCl assays of the extracts derived by fractionated extraction confirmed that they are rich in structurally diverse polyphenols (47 analytes, content up to 650.8 mg GAE/g dw) with proanthocyanidins (83.3–358.2 mg CYE/g) dominating in C. bullatus and C. zabelii, and flavonoids (53.4–147.8 mg/g) in C. integerrimus. In chemical in vitro tests of pro-inflammatory enzymes (lipoxygenase, hyaluronidase) inhibition and antioxidant activity (DPPH, FRAP), the extracts effects were dose-, phenolic- and extraction solvent-dependent. The most promising polyphenolic extracts were demonstrated to be effective antioxidants in a biological model of human blood plasma—at in vivo-relevant levels (1–5 µg/mL) they normalized/enhanced the non-enzymatic antioxidant capacity of plasma and effectively prevented peroxynitrite-induced oxidative/nitrative damage of plasma proteins and lipids. As demonstrated in cytotoxicity tests, the extracts were safe—they did not affect viability of human peripheral blood mononuclear cells. In conclusion, Cotoneaster leaves may be useful in development of natural-based products, supporting the treatment of oxidative stress/inflammation-related chronic diseases, including cardiovascular disorders.
Agnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Anna Marchelak; Malgorzata Sopinska; Pawel Ciszewski; Pawel Nowak; Monika A. Olszewska. Polyphenol-Rich Extracts from Cotoneaster Leaves Inhibit Pro-Inflammatory Enzymes and Protect Human Plasma Components against Oxidative Stress In Vitro. Molecules 2018, 23, 2472 .
AMA StyleAgnieszka Kicel, Joanna Kolodziejczyk-Czepas, Aleksandra Owczarek, Anna Marchelak, Malgorzata Sopinska, Pawel Ciszewski, Pawel Nowak, Monika A. Olszewska. Polyphenol-Rich Extracts from Cotoneaster Leaves Inhibit Pro-Inflammatory Enzymes and Protect Human Plasma Components against Oxidative Stress In Vitro. Molecules. 2018; 23 (10):2472.
Chicago/Turabian StyleAgnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Anna Marchelak; Malgorzata Sopinska; Pawel Ciszewski; Pawel Nowak; Monika A. Olszewska. 2018. "Polyphenol-Rich Extracts from Cotoneaster Leaves Inhibit Pro-Inflammatory Enzymes and Protect Human Plasma Components against Oxidative Stress In Vitro." Molecules 23, no. 10: 2472.
Plasmin (EC 3.4.21.7) is a key enzyme of the fibrinolytic system, responsible for the degradation of fibrin clot and maintaining blood fluidity. Hence, alterations of the fibrinolytic capacity of blood plasma may contribute to thrombotic or bleeding complications. The aim of this study was to determine effects of a bufadienolide-rich fraction, isolated from roots of Kalanchoe daigremontiana (0.05–50 μg/ml) on enzymatic properties of plasmin. Hydrolysis of a synthetic substrate S-2251 (H-D-Valyl-l-leucyl-l-lysine-p-nitroaniline dihydrochloride) by plasmin revealed that the bufadienolide-rich fraction had a diverse effect on this enzyme, dependently on the concentration range. While the lower concentrations of the examined fraction (0.05–2.5 μg/ml) significantly enhanced the amidolytic activity of plasmin, at 25–50 μg/ml concentrations, the enzyme was evidently inhibited (by about 60%). The Lineweaver-Burk plot indicated on an uncompetitive inhibition of plasmin. Inhibitory effects (up to 80%) were also found in the streptokinase-induced plasminogen activation to plasmin. Docking results suggest that only some of compounds (mostly bersaldegenin 1-acetate (10), bryotoxin (13) and hovetrichoside C (17)) were bound to plasminogen/plasmin, depending on the presence or absence of the substrate in the active site. The obtained findings suggest allosteric regulation of plasminogen activation and plasmin activity by components of the examined fraction.
Joanna Kolodziejczyk-Czepas; Bartłomiej Pasiński; Michal B. Ponczek; Barbara Moniuszko-Szajwaj; Mariusz Kowalczyk; Łukasz Pecio; Pawel Nowak; Anna Stochmal. Bufadienolides from Kalanchoe daigremontiana modulate the enzymatic activity of plasmin - In vitro and in silico analyses. International Journal of Biological Macromolecules 2018, 120, 1591 -1600.
AMA StyleJoanna Kolodziejczyk-Czepas, Bartłomiej Pasiński, Michal B. Ponczek, Barbara Moniuszko-Szajwaj, Mariusz Kowalczyk, Łukasz Pecio, Pawel Nowak, Anna Stochmal. Bufadienolides from Kalanchoe daigremontiana modulate the enzymatic activity of plasmin - In vitro and in silico analyses. International Journal of Biological Macromolecules. 2018; 120 ():1591-1600.
Chicago/Turabian StyleJoanna Kolodziejczyk-Czepas; Bartłomiej Pasiński; Michal B. Ponczek; Barbara Moniuszko-Szajwaj; Mariusz Kowalczyk; Łukasz Pecio; Pawel Nowak; Anna Stochmal. 2018. "Bufadienolides from Kalanchoe daigremontiana modulate the enzymatic activity of plasmin - In vitro and in silico analyses." International Journal of Biological Macromolecules 120, no. : 1591-1600.
The phytochemical investigation of the whole plant extracts of Herniaria glabra L. (Caryophyllaceae) led to the identification and isolation of four known flavonoids, one known and three undescribed maltol derivatives, and benzyl β-gentiobioside. The structures were established by extensive 1D and 2D NMR spectroscopic analyses, as well as HRESIMS data. For the first time in Herniaria genus, as well as in Caryophylaceae family the presence of apiorutin {quercetin 3-O-[(D-apio-β-d-furanosyl-(1 → 2)-O-[-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside]} and licoagroside B {maltol 3-O-[6-O-(3-hydroxy-3-methylglutaroyl)]-β-d-glucopyranoside} were revealed. Additionally, antioxidant actions of apiorutin, rutin, narcissin (isorhamentin 3-O-β-d-rutinoside) and licoagroside B were assessed in human blood plasma, exposed to the peroxynitrite-induced oxidative stress in vitro. The isolates partly reduced oxidative (oxidation of thiol groups) and nitrative (tyrosine nitration) damage to blood plasma proteins, decreased plasma lipid peroxidation as well as enhanced the non-enzymatic antioxidant capacity of blood plasma. No cytotoxicity of the examined substances towards peripheral blood mononuclear cells was found.
Solomiia Kozachok; Łukasz Pecio; Joanna Kolodziejczyk-Czepas; Svitlana Marchyshyn; Pawel Nowak; Jaroslaw Moldoch; Wieslaw Oleszek. γ-Pyrone compounds: flavonoids and maltol glucoside derivatives from Herniaria glabra L. collected in the Ternopil region of the Ukraine. Phytochemistry 2018, 152, 213 -222.
AMA StyleSolomiia Kozachok, Łukasz Pecio, Joanna Kolodziejczyk-Czepas, Svitlana Marchyshyn, Pawel Nowak, Jaroslaw Moldoch, Wieslaw Oleszek. γ-Pyrone compounds: flavonoids and maltol glucoside derivatives from Herniaria glabra L. collected in the Ternopil region of the Ukraine. Phytochemistry. 2018; 152 ():213-222.
Chicago/Turabian StyleSolomiia Kozachok; Łukasz Pecio; Joanna Kolodziejczyk-Czepas; Svitlana Marchyshyn; Pawel Nowak; Jaroslaw Moldoch; Wieslaw Oleszek. 2018. "γ-Pyrone compounds: flavonoids and maltol glucoside derivatives from Herniaria glabra L. collected in the Ternopil region of the Ukraine." Phytochemistry 152, no. : 213-222.
Objective The study covers an evaluation of the influence of extracts (1–50 μg/ml), isolated from aerial parts of nine Trifolium L. species (i.e. T. alexandrinum, T. fragiferum, T. hybridum, T. incarnatum, T. pallidum, T. pratense, T. resupinatum var. majus, T. resupinatum var. resupinatum and T. scabrum) on haemostatic properties of blood plasma. Methods The clot formation and fibrinolysis assay (CFF), blood clotting times, the extrinsic and intrinsic coagulation pathway-dependent polymerization of plasma fibrin were measured. The effects of plant extracts on amidolytic activity of thrombin were also evaluated and compared with argatroban, an antithrombotic drug. Cytotoxicity was assessed in a model of blood platelets and as the viability of peripheral blood mononuclear cells. Key findings While no changes in blood clotting times or fibrinolytic properties of blood plasma were found, some fractions impaired the blood plasma coagulation induced by the intrinsic coagulation pathway. Reduction in the maximal velocity of fibrin polymerization was also observed in the clot formation and fibrinolysis assay. No cytotoxicity of Trifolium extracts towards the investigated cells was recorded. Conclusions The most efficient anticoagulant activity in plasma was found for T. fragiferum and T. incarnatum extracts, while the T. alexandrinum fraction was the most effective inhibitor of thrombin amidolytic activity.
Joanna Kolodziejczyk-Czepas; Malgorzata Sieradzka; Barbara Moniuszko-Szajwaj; Pawel Nowak; Wieslaw Oleszek; Anna Stochmal. Phenolic fractions from nine Trifolium species modulate the coagulant properties of blood plasma in vitro without cytotoxicity towards blood cells. Journal of Pharmacy and Pharmacology 2018, 70, 413 -425.
AMA StyleJoanna Kolodziejczyk-Czepas, Malgorzata Sieradzka, Barbara Moniuszko-Szajwaj, Pawel Nowak, Wieslaw Oleszek, Anna Stochmal. Phenolic fractions from nine Trifolium species modulate the coagulant properties of blood plasma in vitro without cytotoxicity towards blood cells. Journal of Pharmacy and Pharmacology. 2018; 70 (3):413-425.
Chicago/Turabian StyleJoanna Kolodziejczyk-Czepas; Malgorzata Sieradzka; Barbara Moniuszko-Szajwaj; Pawel Nowak; Wieslaw Oleszek; Anna Stochmal. 2018. "Phenolic fractions from nine Trifolium species modulate the coagulant properties of blood plasma in vitro without cytotoxicity towards blood cells." Journal of Pharmacy and Pharmacology 70, no. 3: 413-425.
Michał Bijak; Ewelina Synowiec; Jacek Grębowski; Joanna Kołodziejczyk-Czepas; Michał Ponczek. Methods of detecting biological materials. CBRN. Security Manager Handbook 2018, 1 .
AMA StyleMichał Bijak, Ewelina Synowiec, Jacek Grębowski, Joanna Kołodziejczyk-Czepas, Michał Ponczek. Methods of detecting biological materials. CBRN. Security Manager Handbook. 2018; ():1.
Chicago/Turabian StyleMichał Bijak; Ewelina Synowiec; Jacek Grębowski; Joanna Kołodziejczyk-Czepas; Michał Ponczek. 2018. "Methods of detecting biological materials." CBRN. Security Manager Handbook , no. : 1.
Magdalena Matczak; Anna Marchelak; Piotr Michel; Aleksandra Owczarek; Anna Piszczan; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Monika Olszewska. Sorbus domestica L. leaf extracts as functional products: phytochemical profiling, cellular safety, pro-inflammatory enzymes inhibition and protective effects against oxidative stress in vitro. Journal of Functional Foods 2018, 40, 207 -218.
AMA StyleMagdalena Matczak, Anna Marchelak, Piotr Michel, Aleksandra Owczarek, Anna Piszczan, Joanna Kolodziejczyk-Czepas, Pawel Nowak, Monika Olszewska. Sorbus domestica L. leaf extracts as functional products: phytochemical profiling, cellular safety, pro-inflammatory enzymes inhibition and protective effects against oxidative stress in vitro. Journal of Functional Foods. 2018; 40 ():207-218.
Chicago/Turabian StyleMagdalena Matczak; Anna Marchelak; Piotr Michel; Aleksandra Owczarek; Anna Piszczan; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Monika Olszewska. 2018. "Sorbus domestica L. leaf extracts as functional products: phytochemical profiling, cellular safety, pro-inflammatory enzymes inhibition and protective effects against oxidative stress in vitro." Journal of Functional Foods 40, no. : 207-218.
This in vitro study provides a new insight into the phytochemical, antioxidant, antiplatelet and cytotoxic profiles of commercial trans-clovamide and clovamide-rich extracts, isolated from aerial parts of three Trifolium species (i.e. T. clypeatum L., T. obscurum Savi and T. squarrosum L.). Under inorganic experimental conditions, the EC50 for the investigated substances varied between 4.87 (clovamide) and 7.91 μg/ml (T. clypeatum) according to the DPPH(•) assay. The most effective 1 mM ONOO(-) scavenger was clovamide (IC50 = 19.29 μg/ml), and the weakest was the T. obsucurum extract (IC50 = 41.79 μg/ml). The antioxidant action of the examined substances (1-50 μg/ml) was also evaluated in blood plasma (under 100 μM ONOO(-)-induced oxidative stress) using 3-nitrotyrosine, protein thiol groups, lipid hydroperoxides and thiobarbituric acid-reactive substances as biomarkers. All examined substances were more effective in limiting the oxidative damage to blood plasma components than the nitrative damage. Their anti-nitrative action was statistically significant only at the highest concentration (50 μg/ml). Measurements of platelet adhesion and aggregation found the compounds to have moderate antiplatelet properties of up to 20% inhibition of platelet adhesion at concentrations of 1-5 μg/ml. Additionally, clovamide and Trifolium extracts demonstrated no cytotoxicity towards blood platelets or peripheral blood mononuclear cells.
Joanna Kolodziejczyk-Czepas; Justyna Krzyżanowska-Kowalczyk; Malgorzata Sieradzka; Pawel Nowak; Anna Stochmal. Clovamide and clovamide-rich extracts of three Trifolium species as antioxidants and moderate antiplatelet agents in vitro. Phytochemistry 2017, 143, 54 -63.
AMA StyleJoanna Kolodziejczyk-Czepas, Justyna Krzyżanowska-Kowalczyk, Malgorzata Sieradzka, Pawel Nowak, Anna Stochmal. Clovamide and clovamide-rich extracts of three Trifolium species as antioxidants and moderate antiplatelet agents in vitro. Phytochemistry. 2017; 143 ():54-63.
Chicago/Turabian StyleJoanna Kolodziejczyk-Czepas; Justyna Krzyżanowska-Kowalczyk; Malgorzata Sieradzka; Pawel Nowak; Anna Stochmal. 2017. "Clovamide and clovamide-rich extracts of three Trifolium species as antioxidants and moderate antiplatelet agents in vitro." Phytochemistry 143, no. : 54-63.