Background: Green extraction involves using green solvents, such as water, to reduce energy consumption, avoid health and environmental hazards and induce the quality and quantity of the extract. Date palm fruits are a vital source of food and medicinal activities, as they contain a high diversity of phytochemicals, mainly phenolic and flavonoid compounds. The main aim of this study is to investigate the use of water as a green solvent, when assisted by different ultrasonic frequencies, in the extraction of four different cultivars of date palm fruits, by evaluating the phenolic and flavonoid composition as well as the antioxidant capacity of the extract. Methods: Four date palm fruits’ cultivars (Agwa, Anbarah, Khalas, and Reziz) were extracted using conventional methods (by water and ethanol) and by ultrasonic means, using two frequencies, 28 and 40 kHz, and applying temperatures (30, 45, and 60 °C), also measuring extraction times (20, 40, 60 min.). Response surface methodology was used for the statistical analysis, applying three factors (temperature, time, and ultrasonic frequency), four responses (total phenolic content, total flavonoid content, FRAP, and ABTS), and four cultivars (categories). Results: Conventional water extraction obtained minimal phenolic and flavonoid compounds (up to 52% of ethanol extraction). This percent improved to reach 60% when heat was utilized. The application of ultrasonic frequencies significantly enhanced the extraction of phenolics/flavonoids and the antioxidant ability of the extract to nearly 90% and 80%, respectively. The use of 40 kHz ultrasonic power managed to extract more phenolic and flavonoid components; however, the antioxidant capacities of the extract were less than when the 28 kHz power was utilized. Agwa and Khalas demonstrated themselves to be the best cultivars for ultrasonic-assisted extraction, depending on the results of the optimized responses. Conclusion: This study could be implemented in the industry to produce date palm fruits’ enriched extracts with phenolic and flavonoid components and/or antioxidants.

The fruit thinning process is employed for the production of high quality large-sized fruits and prevent the production of compact bunches. It is also one way to reduce the alternate bearing habits in date palm. In this study, seven thinning treatments (i.e. without spraying water after pollination, spraying water at 3 minutes after 3, 4 and 5 h, spraying Ethephon at 0, 500 and 1000 ppm after ten days from pollination) are used for Khalas and Ruzeiz date palm cultivars. The factorial experiment in a randomized completely block design with three replicates was done. The results reveal that, spraying water after mechanical pollination has reduced fruit set% and increased fruit shees%. Most thinning treatments reduced fruit yield/palm in both Khalas and Ruzeiz. Spraying water after 5 h enhanced fruit quality compared with the other thinning treatments in besr and tamr stages. Spraying with ethephon at 1000 ppm gave the increased sugars content and TSS, whereas reduced the moisture content in besr stage. Spraying water after 5 h from mechanical pollination or Ethephon at 1000 ppm after 10 days are suitable for obtaining economic yield with best fruit quality.

Sustainable agriculture is essential to attain food security and address increasing climate change concerns. Population, abiotic and biotic stresses and limited agricultural water supplies and land are important barriers to cereal crops production, in addition to extreme weather. These factors influence their quality and productivity. Therefore, there is a pressing need for effective methods of food production under these conditions. For many individuals in low- and middle-income nations, cereals represent a key food source. Developing cereal crops with enhanced flexibility, high yields, and the ability to tolerate these biotic and abiotic problems is therefore essential. Modern OMICS techniques, next-generation sequencing, bioinformatics tools and the most recent improvements in genome editing tools (GET) have made targeted mutagenesis conceivable. By altering a crop variety's endogenous genome, which is free of any foreign genes, genome editing (GE) enhances the crop variety. Therefore, crops that have undergone GE but have not integrated foreign genes are not considered genetically modified organisms (GMOs) in a number of countries. GE is being used by researchers to promote the nutritional value of cereal crops and increase their tolerance to biotic and abiotic stresses. This review critically discusses the activities of GET, the role of bioinformatics tools and its application to sustainable agriculture for cereal crops.