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The hydroalcoholic extract (MIT) of Micromeria imbricata (Forssk.) growing in Saudi Arabia in addition to the chloroform (MIC) and n-butanol (MIB) fractions were investigated for the first time using UPLC-ESI-MS/MS. The analysis revealed the tentative identification of fifty-eight compounds including three organic acids, twenty-five phenolic compounds, three coumarins, two anthocyanins, twenty-one flavonoids, three terpenes, and one miscellaneous. Moreover, the therapeutic potential of M. imbricata (MIT) and its fractions (MIC and MIB) were determined by in vitro evaluation of their cytotoxic, antioxidant, and anti-obesity characteristics. The MIT extract showed the highest phenolic (125.23 ± 0.87 mg gallic acid equivalent/100 gm extract) and flavonoid (112.24 ± 2.45 mg quercetin equivalent/100 gm extract) contents followed by n-butanol and chloroform fractions. The MIT extract revealed a potent cytotoxic activity against HepG-2 (Hepatocellular carcinoma) and MCF-7 (Breast carcinoma) with IC50 28.5 ± 2.0 and 35.2 ± 1.2 µg/mL, respectively. Additionally, the tested hydroalcoholic extract exhibited a significant DPPH scavenging activity with SC50 28.4 ± 1.2 µg/mL and a remarkable lipase inhibitory activity with IC50 54.2 ± 1.2 µg/mL. In conclusion, the current study presents the first insights into the phytochemical constituents and pharmacological properties of M. imbricata extract and its chloroform and n-butanol fractions. The results revealed that M. imbricata hydroalcoholic extract might be a prolific source of bioactive constituents with potent antioxidant, cytotoxic and anti-obesity potential. It might be a natural alternative therapy and nutritional strategy for obesity treatment.
Hanan M. Al-Yousef; Omer I. Fantoukh; May A. El-Sayed; Musarat Amina; Rasha Adel; Wafaa H.B. Hassan; Sahar Abdelaziz. Metabolic profiling and biological activities of the aerial parts of Micromeria imbricata Forssk. growing in Saudi Arabia. Saudi Journal of Biological Sciences 2021, 1 .
AMA StyleHanan M. Al-Yousef, Omer I. Fantoukh, May A. El-Sayed, Musarat Amina, Rasha Adel, Wafaa H.B. Hassan, Sahar Abdelaziz. Metabolic profiling and biological activities of the aerial parts of Micromeria imbricata Forssk. growing in Saudi Arabia. Saudi Journal of Biological Sciences. 2021; ():1.
Chicago/Turabian StyleHanan M. Al-Yousef; Omer I. Fantoukh; May A. El-Sayed; Musarat Amina; Rasha Adel; Wafaa H.B. Hassan; Sahar Abdelaziz. 2021. "Metabolic profiling and biological activities of the aerial parts of Micromeria imbricata Forssk. growing in Saudi Arabia." Saudi Journal of Biological Sciences , no. : 1.
Phytochemical and pharmacological investigations of Elettaria cardamomum L. were mostly focused on its essential oil and organic crude extracts with little attention on its aqueous extracts. Therefore, the current study aimed to investigate the phytochemical profile, in vitro antiobesity, and antidiabetic activities of the aqueous extract of Elettaria cardamomum L. family Zingiberaceae. UPLC-ESI-MS/MS analysis is used for the aqueous extract characterization in both ionization modes. The analysis revealed the tentative identification of forty-seven compounds based on their MS/MS fragmentation pattern and comparison with the reported data. The identified compounds include eight flavones, thirteen phenolic and nonphenolic acids, one coumarin, and nineteen anthocyanins. Moreover, the in vitro antiobesity and antidiabetic activities were also studied. The results showed that E. cardamomum L. aqueous extract inhibits pancreatic lipase and α-amylase enzyme in a concentration-dependent manner, as 1 mg/mL extract was able to inhibit pancreatic lipase and α-amylase by 62.25% ± 0.58 (IC50 = 288.75 ± 1.3 µg/mL) and 70.42% ± 1.5 (IC50 = 220.5 ± 1.3 µg/mL), respectively. Conclusively, the current study indicated that the investigated biological activities of Elettaria cardamomum aqueous extract were attributed to the existence of biologically active metabolites such as flavones, phenolic and nonphenolic acids, coumarins, and anthocyanins. Moreover, it proposed that the aqueous cardamom extract can be used as a natural potential source in different pharmaceutical preparations to protect against variable chronic disorders, especially obesity and diabetes. Deeper in vivo investigations, isolation, purification, and structural elucidation of the major active metabolites from cardamom are recommended.
Hanan M. Al-Yousef; Ali S. Alqahtani; Wafaa H. B. Hassan; Afraa Alzoubi; Sahar Abdelaziz. Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits. Journal of Chemistry 2021, 2021, 1 -10.
AMA StyleHanan M. Al-Yousef, Ali S. Alqahtani, Wafaa H. B. Hassan, Afraa Alzoubi, Sahar Abdelaziz. Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits. Journal of Chemistry. 2021; 2021 ():1-10.
Chicago/Turabian StyleHanan M. Al-Yousef; Ali S. Alqahtani; Wafaa H. B. Hassan; Afraa Alzoubi; Sahar Abdelaziz. 2021. "Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits." Journal of Chemistry 2021, no. : 1-10.
Cyanobacteria, also known as blue-green algae, are known to be a rich source of secondary metabolites with diverse chemical structures and biological activities. Over 1100 natural constituents of cyanobacterial origin have been reported in the literature. These include various natural biologically active constituents with antimicrobial, antiviral, immunosuppressive, and anticancer activities. Terrestrial cyanobacteria of genus Fischerella (Family Fischerellaceae), belonging to order Stigonematales have recently received enormous attention from the researchers due to the presence of vast array bioactive compounds. Fischerella species are filamentous cyanobacteria with creeping thallus and sheath in filaments. Several species within the genus are known to produce interesting bioactive constituents. Perhaps the best characterized are F. ambigua and F. muscicola, which have been reported to produce antibacterial, antifungal and antialgal isonitrile containing indole alkaloids, such as hapalindoles, ambiguine isonitriles, fischerindoles, and wetwitindolinones. Keeping in mind these resources, a detailed literature survey on a cultured, fresh water and terrestrial cyanobacterium Fischerella ambigua (Nӓg.) Gom. has been carried out. The current review describes the chemical constituents isolated from different extracts of Fischerella ambigua, as well as their biological activities.
Hanan M. Al-Yousef; Musarat Amina. Phytoconstituents and pharmacological activities of cyanobacterium Fischerella ambigua. Arabian Journal of Chemistry 2021, 14, 103153 .
AMA StyleHanan M. Al-Yousef, Musarat Amina. Phytoconstituents and pharmacological activities of cyanobacterium Fischerella ambigua. Arabian Journal of Chemistry. 2021; 14 (6):103153.
Chicago/Turabian StyleHanan M. Al-Yousef; Musarat Amina. 2021. "Phytoconstituents and pharmacological activities of cyanobacterium Fischerella ambigua." Arabian Journal of Chemistry 14, no. 6: 103153.
To investigate the effect of the ethyl acetate fraction of the aerial parts of E. paralias L. F. Euphorbiaceae on nephroprotective, cytotoxic, and antioxidant. different spectroscopic and spectrophotometric methods were applied to identify phytoconstituents. The nephroprotective potential of E. paralias ethyl acetate fraction (Ep EtOAc) was evaluated in male rats with thioacetamide-induced kidney injury, as wll as cytotoxic activity was evaluated using a viability assay, and the antioxidant activity was evaluated using the DPPH method. Results: quantitative estimation of total phenolics and flavonoids of E. paralias was performed using unique spectrophotometric methods. The polyphenolic compounds gallic acid (1), ellagic acid (2), kaempferol-3-O-(6″-O-galloyl-β-D-glucopyranoside) (3), quercetin-3-O-β-D-glucopyranoside (4) and quercetin-3-O-β-D-arabinoside (5) were isolated from the ethyl acetate fraction of the aerial parts of E. paralias. The thioacetamide administration resulted in marked nephrotoxicity, but pretreatment with Ep EtOAc significantly attenuated the nephrotoxicity through alteration of kidney biomarkers, thereby improving the redox status of the tissue and restoring serum biochemical parameters nearly to normal levels. This study revealed a significant cytotoxic and strong antioxidant effect. Conclusion: we conclude that the Ep EtOAc may be used in the future as nephroprotective, cytotoxic, and antioxidant agent derived from a natural source.
Hanan M. Al-Yousef; Ali S. Alqahtani; Afaf S.A. Ghani; Sayed A. El-Toumy; Wagdi I.A. El-Dougdoug; Wafaa H.B. Hassan; Hanaa M. Hassan. Nephroprotective, cytotoxic and antioxidant activities of Euphorbia paralias. Saudi Journal of Biological Sciences 2020, 28, 785 -792.
AMA StyleHanan M. Al-Yousef, Ali S. Alqahtani, Afaf S.A. Ghani, Sayed A. El-Toumy, Wagdi I.A. El-Dougdoug, Wafaa H.B. Hassan, Hanaa M. Hassan. Nephroprotective, cytotoxic and antioxidant activities of Euphorbia paralias. Saudi Journal of Biological Sciences. 2020; 28 (1):785-792.
Chicago/Turabian StyleHanan M. Al-Yousef; Ali S. Alqahtani; Afaf S.A. Ghani; Sayed A. El-Toumy; Wagdi I.A. El-Dougdoug; Wafaa H.B. Hassan; Hanaa M. Hassan. 2020. "Nephroprotective, cytotoxic and antioxidant activities of Euphorbia paralias." Saudi Journal of Biological Sciences 28, no. 1: 785-792.
In continuation of our work on genus Euphorbia, the phytochemical investigation of ethyl acetate fraction of the aerial parts of Euphorbia geniculata Ortega (Eg) family Euphorbiaceae revealed the isolation and identification of eight polyphenolic compounds; gallic acid (1), ellagic acid (2), quercetin-3-O-rutinoside) (rutin) (3), quercetin-3-O-glucopyranoside-7-O-rhamnoside (4), quercetin-3-O-rhamnoside (5), quercetin-3-O-β-D- glucopyranoside (6), and quercetin-3-O-β-D-arabinoside (7) and quercetin (8) using spectrophotometric and physicochemical analysis. Quantitative estimation of total flavonoids and phenolics contents were carried out for total methanolic extract. Biological activities of ethyl acetate fraction including nephroprotective and antioxidant activities were evaluated for the first time. The nephroprotective potential of Eg was evaluated in male rats with thioacetamide induced kidney injury. Antioxidant activity was evaluated using DPPH method. Results; Quantitative estimation of total phenolics and flavonoids content in the total methanolic extract of Eg revealed high phenolics content (285.4 mg GAE /gm extract) in comparison with flavonoids content (36.9 mg RE /gm extract). Isolation and characterization of eight polyphenolic compounds. The nephroprotictive activity was studied using thioacetamide as nephrotoxicant agent resulted in marked nephrotoxicity. While pretreatment of rats with ethyl acetate fraction of Eg significantly attenuated the nephrotoxicity through alteration of kidney biomarkers, improving the redox status of the tissue and so brought the serum biochemical parameters nearly toward the normal levels. The study revealed significant antioxidant activity of Eg in comparison with ascorbic acid. Conclusion; The results suggested that E. geniculata Ortega ethyl acetate fraction could be used in future therapy as nephroprotective and antioxidant drugs of natural source.
Hanan M. Al-Yousef; Ali S. Alqahtani; Afaf E. Abdel Ghani; Sayed A. El-Toumy; Wagdi I.A. El-Dougdoug; Wafaa H.B. Hassan; Hanaa M. Hassan. Nephroprotective and antioxidant activities of ethyl acetate fraction of Euphorbia geniculata Ortega family Euphorbiaceae. Arabian Journal of Chemistry 2020, 13, 7843 -7850.
AMA StyleHanan M. Al-Yousef, Ali S. Alqahtani, Afaf E. Abdel Ghani, Sayed A. El-Toumy, Wagdi I.A. El-Dougdoug, Wafaa H.B. Hassan, Hanaa M. Hassan. Nephroprotective and antioxidant activities of ethyl acetate fraction of Euphorbia geniculata Ortega family Euphorbiaceae. Arabian Journal of Chemistry. 2020; 13 (11):7843-7850.
Chicago/Turabian StyleHanan M. Al-Yousef; Ali S. Alqahtani; Afaf E. Abdel Ghani; Sayed A. El-Toumy; Wagdi I.A. El-Dougdoug; Wafaa H.B. Hassan; Hanaa M. Hassan. 2020. "Nephroprotective and antioxidant activities of ethyl acetate fraction of Euphorbia geniculata Ortega family Euphorbiaceae." Arabian Journal of Chemistry 13, no. 11: 7843-7850.
In the brain, propionic acid (PA) can cross cell membranes and accumulate within cells, leading to intracellular acidification, which may alter neurotransmitter release (NT), communication between neurons, and behavior. Such elevation in levels of PA constitutes a neurodevelopmental metabolic disorder called propionic acidemia, which could clinically manifest as autism. The purpose of this study was to investigate the protective effects of different fractions of bee pollen (BP) on PA‐induced autism in rats, and to evaluate their effects on the expression of liver and renal biomarkers. Groups of rats received treatments of different fractions of BP at a dose of 250 mg/kg of body weight/day for a period of 1 month. Normal control group I and group II were orally administered with phosphate‐buffered saline and propionic acid, respectively, for 3 days. BP contains various health‐promoting phenolic components. Different fractions of BP administered pre‐ and post‐treatment with PA showed significant reduction in the levels of liver and renal biomarkers (p < .05). Also, a significant enhancement in the levels of glutathione S‐transferase (GST), catalase CAT), and ascorbic acid (VIT C) was observed. Supplementation with BP significantly reduced biochemical changes in the liver, kidneys, and brain of rats with PA‐induced toxicity. It exhibited protective effects against oxidative damage and reactive oxygen species produced by PA‐induced adverse reactions in rats. Taken together, our study shows that BP possesses protective effects in PA‐induced liver and kidney damage.
Huda S. Al‐Salem; Hanan M. Al‐Yousef; Abdelkader E. Ashour; Atallah F. Ahmed; Musarat Amina; Iman S. Issa; Ramesa Shafi Bhat. Antioxidant and hepatorenal protective effects of bee pollen fractions against propionic acid‐induced autistic feature in rats. Food Science & Nutrition 2020, 8, 5114 -5127.
AMA StyleHuda S. Al‐Salem, Hanan M. Al‐Yousef, Abdelkader E. Ashour, Atallah F. Ahmed, Musarat Amina, Iman S. Issa, Ramesa Shafi Bhat. Antioxidant and hepatorenal protective effects of bee pollen fractions against propionic acid‐induced autistic feature in rats. Food Science & Nutrition. 2020; 8 (9):5114-5127.
Chicago/Turabian StyleHuda S. Al‐Salem; Hanan M. Al‐Yousef; Abdelkader E. Ashour; Atallah F. Ahmed; Musarat Amina; Iman S. Issa; Ramesa Shafi Bhat. 2020. "Antioxidant and hepatorenal protective effects of bee pollen fractions against propionic acid‐induced autistic feature in rats." Food Science & Nutrition 8, no. 9: 5114-5127.
Parkinsonia aculeata L. growing in Saudi Arabia was investigated for its phytochemical profile, antioxidant, and cytotoxic properties. UPLC-ESI-MS/MS was employed as a powerful technique for the characterization of secondary metabolites from a hydroalcoholic extract, dichloromethane, and ethyl acetate fractions of P. aculeata L. aerial parts. Sixty-nine compounds (flavonoids, anthocyanins, phenolics and fatty acids) were detected and characterized; flavonoids were the abundant components in the analyzed samples. The dichloromethane fraction was rich in phenolics as vanillic acid hexoside, flavonols as 3,7-dimthylquercetin, and flavones as 3′-hydroxymelanettin. However, the ethyl acetate fraction was rich in flavonoid-C-glycosides as luteolin-8-C-β-D-glucoside (orientin) and apigenin-8-C-glucoside (vitexin), flavonoid- O, C-diglycosides such as luteolin 7-O-[6′'-dihydrogalloyl]-glucosyl-8-C-pentosyl-(1 → 2)-glucoside and 2′'-O-rhamnosyl isoorientin. These compounds were identified for the first time in dichloromethane and ethyl acetate fractions of Saudi P. aculeata L. Additionally, all the samples were assessed for antioxidant activity using DPPH radical scavenging method and for cytotoxic activity through MTT assay. Accordingly, the most active fraction was the ethyl acetate which showed the highest antioxidant activity (SC50 = 57.4 ± 1.2 μg/mL) compared with the positive control, ascorbic acid (SC50 = 12.4 ± 0.5 μg/mL) and moderate cytotoxicity against HepG-2 (hepatocellular carcinoma) and MCF-7 (breast carcinoma) cell lines with IC50 = 56.9 ± 3.1 and 95.8 ± 3.8 μg/mL, respectively compared with cisplatin (IC50 = 3.67 ± 0.22 and 5.71 ± 0.57 μg/mL, respectively for both cell lines). The antioxidant and cytotoxic activities may be attributed to the presence of high percentage of phenolic compounds and hydroxylated flavonoids detected in ethyl acetate fraction using UPLS-ESI-MS/MS.
Sahar Abdelaziz; Hanan M. Al Yousef; Ali S. Al-Qahtani; Wafaa H.B. Hassan; Omer I. Fantoukh; May A. El-Sayed. Phytochemical profile, antioxidant and cytotoxic potential of Parkinsonia aculeata L. growing in Saudi Arabia. Saudi Pharmaceutical Journal 2020, 28, 1129 -1137.
AMA StyleSahar Abdelaziz, Hanan M. Al Yousef, Ali S. Al-Qahtani, Wafaa H.B. Hassan, Omer I. Fantoukh, May A. El-Sayed. Phytochemical profile, antioxidant and cytotoxic potential of Parkinsonia aculeata L. growing in Saudi Arabia. Saudi Pharmaceutical Journal. 2020; 28 (9):1129-1137.
Chicago/Turabian StyleSahar Abdelaziz; Hanan M. Al Yousef; Ali S. Al-Qahtani; Wafaa H.B. Hassan; Omer I. Fantoukh; May A. El-Sayed. 2020. "Phytochemical profile, antioxidant and cytotoxic potential of Parkinsonia aculeata L. growing in Saudi Arabia." Saudi Pharmaceutical Journal 28, no. 9: 1129-1137.
The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50 = 50 ± 5.1 μg/mL, and Hibiscus micranthus showed a close effect with IC50 = 60.4 ± 1.7 μg/mL. Hibiscus micranthus showed the most potent effect on HCT-116 (human colon carcinoma) with IC50 = 56 ± 1.9 μg/mL compared with cisplatin (IC50 = 7.53 ± 3.8 μg/mL). HcA and HdA extracts showed weak cytotoxic activity against A-549 and HCT-116 cell lines compared to the other extracts. Eventually, Hibiscus deflersii showed astonishing antidiabetic (IC50 = 56 ± 1.9 μg/mL) and antiobesity (IC50 = 95.45 ± 1.9 μg/mL) activities using in vitro α-amylase inhibitory assay (compared with acarbose (IC50 = 34.71 ± 0.7 μg/mL)) and pancreatic lipase inhibitory assay (compared with orlistat (IC50 = 23.8 ± 0.7 μg/mL)), respectively. In conclusion, these findings are regarded as the first vision of the phytochemical constituents and biological activities of different Hibiscus aqueous extracts. Hibiscus deflersii aqueous extract might be a hopeful origin of functional constituents with anticancer (on A-549 cell line), antidiabetic, and antiobesity activities. It might be a natural alternative remedy and nutritional policy for diabetes and obesity treatment without negative side effects. Isolation of the bioactive phytochemicals from the aqueous extracts of aerial parts of Hibiscus calyphyllus, Hibiscus micranthus, and Hibiscus deflersii and estimation of their biological effects are recommended in further studies.
Hanan M. Al-Yousef; Wafaa H. B. Hassan; Sahar Abdelaziz; Musarat Amina; Rasha Adel; May El-Sayed. UPLC-ESI-MS/MS Profile and Antioxidant, Cytotoxic, Antidiabetic, and Antiobesity Activities of the Aqueous Extracts of Three Different Hibiscus Species. Journal of Chemistry 2020, 2020, 1 -17.
AMA StyleHanan M. Al-Yousef, Wafaa H. B. Hassan, Sahar Abdelaziz, Musarat Amina, Rasha Adel, May El-Sayed. UPLC-ESI-MS/MS Profile and Antioxidant, Cytotoxic, Antidiabetic, and Antiobesity Activities of the Aqueous Extracts of Three Different Hibiscus Species. Journal of Chemistry. 2020; 2020 ():1-17.
Chicago/Turabian StyleHanan M. Al-Yousef; Wafaa H. B. Hassan; Sahar Abdelaziz; Musarat Amina; Rasha Adel; May El-Sayed. 2020. "UPLC-ESI-MS/MS Profile and Antioxidant, Cytotoxic, Antidiabetic, and Antiobesity Activities of the Aqueous Extracts of Three Different Hibiscus Species." Journal of Chemistry 2020, no. : 1-17.
Bee pollens are rich source of essential amino acids and are often considered as complete food for human beings. Herein, we exploited the potential reducing abilities of Bee pollens extract for the eco-friendly preparation of silver nanoparticles (AgNPs-G). The resulting NPs were characterized using a combination of microscopic and spectroscopic techniques. The analyses confirm the formation of spherical Ag NPs. AgNPs-G obtained from the aqueous extract of bee pollens was used to study their antibacterial properties against Gram-positive and Gram-negative microbes using the Minimum Inhibitory Concentration 50 (MIC50) method. The antibacterial properties of AgNPs-G were compared to the properties of chemically synthesized Ag NPs (AgNPs-C) using sodium borohydride as a reducing agent. The green synthesized nanoparticles (AgNPs-G) exhibited a better antibacterial activity against most of the studied strains when compared to the chemically synthesized Ag NPs (AgNPs-C). In addition, the anti-cancer activity of Ag NPs was also studied against human liver and breast carcinoma cell lines by applying MTT-assay. The Ag NPs demonstrated considerable anticancer activity against the studied cell lines and exhibited high IC50 values in both MCF-7 and HepG2 cell lines.
Hanan M. Al-Yousef; Musarat Amina; Ali S. Alqahtani; Mohammed S. Alqahtani; Abdul Malik; Mohammad Rafe Hatshan; Mohammed Rafiq H. Siddiqui; Mujeeb Khan; Mohammed Rafi Shaik; Mohammad Shamsul Ola; Rabbani Syed. Pollen Bee Aqueous Extract-Based Synthesis of Silver Nanoparticles and Evaluation of Their Anti-Cancer and Anti-Bacterial Activities. Processes 2020, 8, 524 .
AMA StyleHanan M. Al-Yousef, Musarat Amina, Ali S. Alqahtani, Mohammed S. Alqahtani, Abdul Malik, Mohammad Rafe Hatshan, Mohammed Rafiq H. Siddiqui, Mujeeb Khan, Mohammed Rafi Shaik, Mohammad Shamsul Ola, Rabbani Syed. Pollen Bee Aqueous Extract-Based Synthesis of Silver Nanoparticles and Evaluation of Their Anti-Cancer and Anti-Bacterial Activities. Processes. 2020; 8 (5):524.
Chicago/Turabian StyleHanan M. Al-Yousef; Musarat Amina; Ali S. Alqahtani; Mohammed S. Alqahtani; Abdul Malik; Mohammad Rafe Hatshan; Mohammed Rafiq H. Siddiqui; Mujeeb Khan; Mohammed Rafi Shaik; Mohammad Shamsul Ola; Rabbani Syed. 2020. "Pollen Bee Aqueous Extract-Based Synthesis of Silver Nanoparticles and Evaluation of Their Anti-Cancer and Anti-Bacterial Activities." Processes 8, no. 5: 524.
Summary The aim of this study was to develop a validated high-performance thin-layer chromatography (HPTLC) procedure for resolution of chemical constituents and identification and quantification of two selected natural anticancer compounds, stigmasterol (PD-1) and cinnamic acid (PD-2), in Pluchea dioscoridis chloroform fraction (PDCF). The chromatographic estimations were conducted on normal HPTLC (20 cm × 10 cm glass-backed silica gel 60 F254) plates with chloroform-methanol-acetic acid (93:5:2, V/V) used as the mobile phase. para-Anisaldehyde was used for the derivatization of the developed plate, and compact spots were scanned at λmax = 513 nm. A well resolved, compact, and intense peaks of PD-1 and PD-2 were recorded at RF = 0.57 and 0.19, respectively. The proposed analytical method for both biomarker compounds was found to be handy, simple, precise, linear (%RSD = 1.03–1.45), accurate (98.91–99.14%), reliable, and sensitive for the analysis of both bio-markers. The LOD/LOQ (ng) for PD-1 and PD-2 were recorded as 38.73/117.37 and 42.58/129.04, respectively, in the linearity range of 200–1400 ng per spot. The obtained result showed maximum quantities of PD-1 and PD-2 (5.36 and 16.98 μg mg−1, respectively). The developed HPTLC was found to be suitable for the routine analysis of these 2 biomarkers in the chloroform fraction of Pluchea dioscoridis and can be further employed in the process quality control of herbal formulations containing the said biomarkers.
Hanan M. Al-Yousef; Tawfeq A. Alhowiriny; Nasir A. Siddiqui; Perwez Alam; Wafaa Hassan Badr Hassan; Musarat Amina. Quantification of two biomarker compounds by a validated high-performance thin-layer chromatographic method from different extracts of Pluchea dioscoridis growing in Saudi Arabia. JPC – Journal of Planar Chromatography – Modern TLC 2019, 32, 243 -249.
AMA StyleHanan M. Al-Yousef, Tawfeq A. Alhowiriny, Nasir A. Siddiqui, Perwez Alam, Wafaa Hassan Badr Hassan, Musarat Amina. Quantification of two biomarker compounds by a validated high-performance thin-layer chromatographic method from different extracts of Pluchea dioscoridis growing in Saudi Arabia. JPC – Journal of Planar Chromatography – Modern TLC. 2019; 32 (3):243-249.
Chicago/Turabian StyleHanan M. Al-Yousef; Tawfeq A. Alhowiriny; Nasir A. Siddiqui; Perwez Alam; Wafaa Hassan Badr Hassan; Musarat Amina. 2019. "Quantification of two biomarker compounds by a validated high-performance thin-layer chromatographic method from different extracts of Pluchea dioscoridis growing in Saudi Arabia." JPC – Journal of Planar Chromatography – Modern TLC 32, no. 3: 243-249.
Quorum sensing is an important regulatory factor of P. aeruginosa virulence induction such as BF, motility, formations of proteases, pyocyanin, and some toxins. The aim of the current study is to detect the effect of the pet.ether extract from onion husk and compound drive from it on quorum sensing and virulence formations of P. aeruginosa. Quorum sensing inhibiting effect of the pet.ether extract of onion husk and a compound drive from it, was evaluated by C. violaceum reporter using dilution method as well as an antioxidant by using DPPH. The efficacious of: Quorum sensing inhibiting on pet.ether fraction and compound derived from it, were investigated for their activities toward biofilm and pyocyanin synthesis as well as motility from P. aeruginosa. The pet.ether fraction and compound derived from it of onion husk exhibited potent antimicrobial, antioxidant and Quorum sensing inhibiting effects. The pet.ether fraction and compound derived from it possesses significant reduction on pyocyanin and biofilm induction of P. aeruginosa. Moreover, they significantly inhibited swimming motilities of P. aeruginosa. For the first time, our study showed the medical importance of Allium cepa L. as antimicrobial, antioxidant as well as Quorum sensing inhibiting and virulence suppressors of P. aeruginosa. Thus, these might emphasized on Allium cepa L as a natural source for attenuating toxins of the Pseudomonas.
Hanan M. Al-Yousef; Ishfaq Ahmad Sheikh. β-Sitosterol derived compound from onion husks non-polar fraction reduces quorum sensing controlled virulence and biofilm production. Saudi Pharmaceutical Journal 2019, 27, 664 -672.
AMA StyleHanan M. Al-Yousef, Ishfaq Ahmad Sheikh. β-Sitosterol derived compound from onion husks non-polar fraction reduces quorum sensing controlled virulence and biofilm production. Saudi Pharmaceutical Journal. 2019; 27 (5):664-672.
Chicago/Turabian StyleHanan M. Al-Yousef; Ishfaq Ahmad Sheikh. 2019. "β-Sitosterol derived compound from onion husks non-polar fraction reduces quorum sensing controlled virulence and biofilm production." Saudi Pharmaceutical Journal 27, no. 5: 664-672.
The genus Hibiscus contains about 275 species of flowering plants widely grown in the tropics and sub-tropics. The available literature revealed that several Hibiscus species exhibited excellent anticancer activity against several cancer cells like lung, breast, and liver. This motivated the authors to explore the anticancer property of other Hibiscus species (Hibiscus calyphyllus, H. deflersii and H. micranthus) along with development of a validated HPTLC method for the concurrent analysis of three anticancer biomarkers (ursolic acid, β-sitosterol and lupeol) in different Hibiscus species. The anticancer activity of various fractions (petroleum ether, toluene, dichloromethane, ethyl acetate and n-butanol) of all the Hibiscus species (aerial parts) were evaluated in vitro against HepG2 and MCF-7 cell lines using MTT assay. The HPTLC analysis was carried out using chloroform and methanol as mobile phase (97:3; v/v) on 20 × 10 cm glass-backed silica gel 60F254 plates and analyzed different phytoconstituents present in all fractions at λ = 575 nm wavelength. Of the tested fractions of H. calyphyllus, H. deflersii and H. micranthus, HdP (H. deflersii petroleum ether fraction) exhibited the most potent cytotoxic effect on HepG2 and MCF-7 (IC50: 14.4 and 11.1 μg/mL, respectively) cell lines. Using the developed HPTLC method a compact and intense peak of ursolic acid, β-sitosterol and lupeol were obtained at Rf = 0.22, 0.39 and 0.51, respectively. The LOD/LOQ (ng) for ursolic acid, β-sitosterol and lupeol were found as 42.30/128.20, 13.20/40.01 and 31.57/95.68, respectively in the linearity range 100–1200 ng/spot. The obtained result showed maximum presence of ursolic acid, β-sitosterol and lupeol (5.50, 11.85 and 7.47 μg/mg, respectively) in HdP which also supported its strong anticancer effect. Our data suggest that H. deflersii petroleum ether fraction (HdP) can be further subjected to the isolation of active cytotoxic phytoconstituents and establishment of their mechanism of action. The maiden developed HPTLC method for concurrent analysis of anticancer biomarkers may be further employed in the in process quality control of herbal formulation containing the said biomarkers.
Perwez Alam; Hanan M. Al-Yousef; Nasir A. Siddiqui; Tawfeq A. Alhowiriny; Saleh I. Alqasoumi; Musarat Amina; Wafaa Hassan Badr Hassan; Sahar Abdelaziz; Rehab H. Abdalla. Anticancer activity and concurrent analysis of ursolic acid, β-sitosterol and lupeol in three different Hibiscus species (aerial parts) by validated HPTLC method. Saudi Pharmaceutical Journal 2018, 26, 1060 -1067.
AMA StylePerwez Alam, Hanan M. Al-Yousef, Nasir A. Siddiqui, Tawfeq A. Alhowiriny, Saleh I. Alqasoumi, Musarat Amina, Wafaa Hassan Badr Hassan, Sahar Abdelaziz, Rehab H. Abdalla. Anticancer activity and concurrent analysis of ursolic acid, β-sitosterol and lupeol in three different Hibiscus species (aerial parts) by validated HPTLC method. Saudi Pharmaceutical Journal. 2018; 26 (7):1060-1067.
Chicago/Turabian StylePerwez Alam; Hanan M. Al-Yousef; Nasir A. Siddiqui; Tawfeq A. Alhowiriny; Saleh I. Alqasoumi; Musarat Amina; Wafaa Hassan Badr Hassan; Sahar Abdelaziz; Rehab H. Abdalla. 2018. "Anticancer activity and concurrent analysis of ursolic acid, β-sitosterol and lupeol in three different Hibiscus species (aerial parts) by validated HPTLC method." Saudi Pharmaceutical Journal 26, no. 7: 1060-1067.
In this study, we have developed a validated high-performance thin-layer chromatographic (HPTLC) method for the simultaneous quantification of two phenolic biomarkers, protocatechuic acid (compound 1) and quercetin 4ʹ-O-β-d-glucopyranoside (compound 2) in antimicrobial and antioxidant active A. cepa ethyl acetate extract (ACEAE). The quantitative analysis was carried out on normal-phase HPTLC (glass-backed silica gel 60 F254) plates with solvents toluene, ethyl acetate, and formic acid in the ratio of 3:6:1, v/v (as the mobile phase). Well-resolved, compact, and intense peaks of compound 1 (RF = 0.56 ± 0.001) and compound 2 (RF = 0.05 ± 0.001) were found at λmax = 275 nm. The linear regression equation / r2 for compound 1 (Y = 8.89X + 250.71 / 0.994) and compound 2 (Y = 6.64X + 209.34 / 0.998) in the concentration range of 100–700 ng spot−1 indicated good linear relationship. The low values of percent relative standard deviation (%RSD) for intra-day / inter-day precision of compound 1 (1.14–1.26 / 1.08–1.23) and compound 2 (0.97–1.18 / 0.93–1.16) suggested that the method is precise. The (%) recoveries for compound 1 / compound 2 were found as 98.07–99.55 / 98.20–99.89 which confirms the good accuracy of the proposed method. The quantities (%w/w) of compound 1 / compound 2 in ACEAE were found as 18.84% / 13.64% of the dried weight of the extract. In vitro 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay showed the promising free radical scavenging activity of ACEAE (69.00 ± 2.99%) and compound 2 (63.86 ± 2.02%) which were comparable to ascorbic acid tested at 400 μg mL−1. ACEAE was found to be highly active against all tested bacterial strains, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa; however, Candida albicans was found to be susceptible to both compound 2 and ACEAE. The presence of compound 2 in high quantity in the ethyl acetate fractions of A. cepa peel (ACEAE) validated its antimicrobial and antioxidant property. The above developed HPTLC method can be further employed in the analysis of these markers in marketed formulations and in the quality control of herbal drugs.
Hanan M. Al-Yousef; Tawfeq A. Alhowiriny; Perwez Alam; Nasir A. Siddiqui; Atallah F. Ahmed; Jawaher H. Al-Qahtani; Musarat Amina. Simultaneous quantification of two phenolic biomarkers by a validated high-performance thin-layer chromatographic method in antimicrobial and antioxidant active ethyl acetate fraction of Allium cepa L. (peel). JPC – Journal of Planar Chromatography – Modern TLC 2017, 30, 510 -515.
AMA StyleHanan M. Al-Yousef, Tawfeq A. Alhowiriny, Perwez Alam, Nasir A. Siddiqui, Atallah F. Ahmed, Jawaher H. Al-Qahtani, Musarat Amina. Simultaneous quantification of two phenolic biomarkers by a validated high-performance thin-layer chromatographic method in antimicrobial and antioxidant active ethyl acetate fraction of Allium cepa L. (peel). JPC – Journal of Planar Chromatography – Modern TLC. 2017; 30 (6):510-515.
Chicago/Turabian StyleHanan M. Al-Yousef; Tawfeq A. Alhowiriny; Perwez Alam; Nasir A. Siddiqui; Atallah F. Ahmed; Jawaher H. Al-Qahtani; Musarat Amina. 2017. "Simultaneous quantification of two phenolic biomarkers by a validated high-performance thin-layer chromatographic method in antimicrobial and antioxidant active ethyl acetate fraction of Allium cepa L. (peel)." JPC – Journal of Planar Chromatography – Modern TLC 30, no. 6: 510-515.
The resistance and pathogenesis of bacteria could be related to their ability to sense and respond to population density, termed quorum sensing (QS). Inhibition of the QS system is considered as a novel strategy for the development of antipathogenic agents, especially for combating drug-resistant bacterial infections. In the present study, the anti-QS activity of Onion peel ethylacetate fraction (ONE) was tested against Chromobacterium violaceum CV12472 and Pseudomonas aeruginosa PAO1. ONE inhibit the QS-mediated virulence factors production such as violacein in C. violaceum and elastase, pyocyanin in P. aeruginosa. Further, the treatment with sub-MICs of ONE significantly inhibited the QS-mediated biofilm formation, EPS (Extracellular polymeric substances) production and swarming motility. Further, quercetin 4′-O-β-D glucopyranoside (QGP) was isolated from ONE and its anti-QS potential was confirmed after observing significant inhibition of QS-controlled virulence factors such as violacein, elastase, pyocyanin and biofilm formation in test pathogens. Molecular docking analysis predicted that QGP should be able to bind at the active sites of Vfr and LasR, and if so blocks the entry of active sites in Vfr and LasR.
Hanan M. Al-Yousef; Atallah F. Ahmed; Nasser A. Al-Shabib; Sameen Laeeq; Rais A. Khan; T. Rehman; Ali Alsalme; Mohamed F. Al-Ajmi; Mohammad S. Khan; Fohad M. Husain. Onion Peel Ethylacetate Fraction and Its Derived Constituent Quercetin 4′-O-β-D Glucopyranoside Attenuates Quorum Sensing Regulated Virulence and Biofilm Formation. Frontiers in Microbiology 2017, 8, 1675 .
AMA StyleHanan M. Al-Yousef, Atallah F. Ahmed, Nasser A. Al-Shabib, Sameen Laeeq, Rais A. Khan, T. Rehman, Ali Alsalme, Mohamed F. Al-Ajmi, Mohammad S. Khan, Fohad M. Husain. Onion Peel Ethylacetate Fraction and Its Derived Constituent Quercetin 4′-O-β-D Glucopyranoside Attenuates Quorum Sensing Regulated Virulence and Biofilm Formation. Frontiers in Microbiology. 2017; 8 ():1675.
Chicago/Turabian StyleHanan M. Al-Yousef; Atallah F. Ahmed; Nasser A. Al-Shabib; Sameen Laeeq; Rais A. Khan; T. Rehman; Ali Alsalme; Mohamed F. Al-Ajmi; Mohammad S. Khan; Fohad M. Husain. 2017. "Onion Peel Ethylacetate Fraction and Its Derived Constituent Quercetin 4′-O-β-D Glucopyranoside Attenuates Quorum Sensing Regulated Virulence and Biofilm Formation." Frontiers in Microbiology 8, no. : 1675.