Global warming and the increase in organic waste from agro-industries create a major problem for the environment. In this sense, microbial fuel cells (MFC) have great potential for the generation of bioelectricity by using organic waste as fuel. This research produced low-cost MFC by using zinc and copper electrodes and taking blueberry waste as fuel. A peak current and voltage of 1.130 ± 0.018 mA and 1.127 ± 0.096 V, respectively, were generated. The pH levels were acid, with peak conductivity values of 233. 94 ± 0.345 mS/cm and the degrees Brix were descending from the first day. The maximum power density was 3.155 ± 0.24 W/cm² at 374.4 mA/cm² current density, and Cándida boidinii was identified by means of molecular biology and bioinformatics techniques. This research gives a new way to generate electricity with this type of waste, generating added value for the companies in this area and helping to reduce global warming.

The enormous environmental problems that arise from organic waste have increased due to the significant population increase worldwide. Microbial fuel cells provide a novel solution for the use of waste as fuel for electricity generation. In this investigation, onion waste was used, and managed to generate maximum peaks of 4.459 ± 0.0608 mA and 0.991 ± 0.02 V of current and voltage, respectively. The conductivity values increased rapidly to 179,987 ± 2859 mS/cm, while the optimal pH in which the most significant current was generated was 6968 ± 0.286, and the ° Brix values decreased rapidly due to the degradation of organic matter. The microbial fuel cells showed a low internal resistance (154,389 ± 5228 Ω), with a power density of 595.69 ± 15.05 mW/cm² at a current density of 6.02 A/cm²; these values are higher than those reported by other authors in the literature. The diffractogram spectra of the onion debris from FTIR show a decrease in the most intense peaks, compared to the initial ones with the final ones. It was possible to identify the species Pseudomona eruginosa, Acinetobacter bereziniae, Stenotrophomonas maltophilia, and Yarrowia lipolytica adhered to the anode electrode at the end of the monitoring using the molecular technique.

Power generation and wastewater treatment are two great challenges for sustainable development. Microbial fuel cells (MFCs) are a sustainable alternative that can generate bioelectricity in the bioremediation process of wastewater. For this reason, the objective of this research was to generate bioelectricity through double-chamber microbial-combustion cell systems from wastewater from the Covicorti Wastewater Treatment Plant (PTARC) in the anodic chamber and electrogenic bacteria such as Stenotrophomonas maltophilia, Acinetobacter bereziniae, and Achromobacteria xylosoxidans in the cathode chamber, respectively. Measurements of the voltage, current, power density, current density, and optical density of the bacteria and biochemical oxygen demand (BOD) were made. In addition, a metagenomic analysis of the wastewater sample was performed. It was shown that the MFC with A. xylosoxidans generated the highest voltage peak (1.01 ± 0.06 V) on day 24, while the MFC with S. maltophilia generated the highest current value (0.71 ± 0.02 mA). The pH levels were slightly alkaline, and the maximum anodic conductivity value was presented by the MFC with A. cerevisiae, with a peak value of 81 ± 2 mS/cm on day 24. On the other hand, a maximum power density and current density of 195,493 ± 4717 mW/m² and 4987 A/cm², respectively, were obtained in the MFC with A. xylosoxidans. Finally, the metagenomic analysis identified the predominant phyla of Proteobacteria present in wastewater samples capable of generating electrical energy as Bacillota, Pseudomonadota, Bacteroidota, Actinomyketone, and Campylobacterota.

The excessive use of fossil sources for the generation of electrical energy and the increase in different organic wastes have caused great damage to the environment; these problems have promoted new ways of generating electricity in an eco-friendly manner using organic waste. In this sense, this research uses single-chamber microbial fuel cells with zinc and copper as electrodes and pineapple waste as fuel (substrate). Current and voltage peaks of 4.95667 ± 0.54775 mA and 0.99 ± 0.03 V were generated on days 16 and 20, respectively, with the substrate operating at an acid pH of 5.21 ± 0.18 and an electrical conductivity of 145.16 ± 9.86 mS/cm at two degrees Brix. Thus, it was also found that the internal resistance of the cells was 865.845 ± 4.726 Ω, and a maximum power density of 513.99 ± 6.54 mW/m² was generated at a current density of 6.123 A/m², and the final FTIR spectrum showed a clear decrease in the initial transmittance peaks. Finally, from the biofilm formed on the anodic electrode, it was possible to molecularly identify the yeast Wickerhamomyces anomalus with 99.82% accuracy. In this way, this research provides a method that companies exporting and importing this fruit may use to generate electrical energy from its waste.

The large amounts of organic waste thrown into the garbage without any productivity, and the increase in the demand for electrical energy worldwide, has led to the search for new eco-friendly ways of generating electricity. Because of this, microbial fuel cells have begun to be used as a technology to generate bioelectricity. The main objective of this research was to generate bioelectricity through banana waste using a low-cost laboratory-scale method, achieving the generation of maximum currents and voltages of 3.71667 ± 0.05304 mA and 1.01 ± 0.017 V, with an optimal pH of 4.023 ± 0.064 and a maximum electrical conductivity of the substrate of 182.333 ± 3.51 µS/cm. The FTIR spectra of the initial and final substrate show a decrease in the peaks belonging to phenolic compounds, alkanes, and alkenes, mainly. The maximum power density was 5736.112 ± 12.62 mW/cm² at a current density of 6.501 A/cm² with a peak voltage of 1006.95 mV. The molecular analysis of the biofilm formed on the anode electrode identified the species Pseudomonas aeruginosa (100%), and Paenalcaligenes suwonensis (99.09%), Klebsiella oxytoca (99.39%) and Raoultella terrigena (99.8%), as the main electricity generators for this type of substrate. This research gives a second use to the fruit with benefits for farmers and companies dedicated to exporting and importing because they can reduce their expenses by using their own waste.

Tuberculosis (TB) is the leading cause of death globally, surpassing HIV. Furthermore, multidrug-resistant and extensively drug-resistant TB have become global public health threats. Care of TB patients starts with quality, accessible, and affordable diagnosis. The study presents a novel technique called nanoparticle-based colorimetric biosensing assay (NCBA) based on the principles of magnetically activated cell enrichment. A total of 1108 sputum samples were subjected to sputum smear microscopy (SSM), NCBA, and standard culture. SSM and NCBA were completed in 20 min; culture was completed in 8 weeks. Results show that NCBA has matching sensitivity of 100.0% and specificity of 99.7% compared to the gold standard culture method at a cost of $0.50/test based on Peruvian conditions. Sputum smear microscopy has 63.87% sensitivity compared to culture. NCBA has the potential of being used in local health clinics as it only requires a microscope that is widely available in many rural areas. Because NCBA could detect low levels of bacterial load comparable to culture, it could be used for rapid and early TB-onset detection. The gain in time is critical as TB is airborne and highly infectious, minimizing contact exposure. Early detection could lead to early treatment, while the patient’s immune system is still high. The low cost makes NCBA affordable and accessible to those who need them the most.