Mapping maize water stress status and monitoring its spatial variability at a farm scale are a prerequisite for precision irrigation. High-resolution multispectral images acquired from an unmanned aerial vehicle (UAV) were used to evaluate the applicability of the data in mapping water stress status of maize under different levels of deficit irrigation at the late vegetative, reproductive and maturation growth stages. Canopy temperature, field air temperature and relative humidity obtained by a handheld infrared thermometer and a portable air temperature/relative humidity meter were used to establish a crop water stress index (CWSI) empirical model under the weather conditions in Ordos, Inner Mongolia, China. Nine vegetation indices (VIs) related to crop water stress were derived from the UAV multispectral imagery and used to establish CWSI inversion models. The results showed that non-water-stressed baseline had significant difference in the reproductive and maturation stages with an increase of 2.1 °C, however, the non-transpiring baseline did not change significantly with an increase of 0.1 °C. The ratio of transformed chlorophyll absorption in reflectance index (TCARI) and renormalized difference vegetation index (RDVI), and the TCARI and soil-adjusted vegetation index (SAVI) had the best correlations with CWSI. R² values were 0.47 and 0.50 for TCARI/RDVI and TCARI/SAVI at the reproductive and maturation stages, respectively; and 0.81 and 0.80 for TCARI/RDVI and TCARI/SAVI at the late reproductive and maturation stages, respectively. Compared to CWSI calculated by on-site measurements, CWSI values retrieved by VI-CWSI regression models established in this study had more abilities to assess the field variability of crop and soil. This study demonstrates the potentiality of using high-resolution UAV multispectral imagery to map maize water stress.

To identify drought-tolerant crop cultivars or achieve a balance between water use and yield, accurate measurements of crop water stress are needed. In this study, the canopy temperature (Tc) of maize at the late vegetative stage was extracted from high-resolution red–green–blue (RGB, 1.25 cm) and thermal (7.8 cm) images taken by an unmanned aerial vehicle (UAV). To reduce the number of parameters for crop water stress monitoring, four simple methods that require only Tc were identified: Tc, degrees above non-stress, standard deviation of Tc, and variation coefficient of Tc. The ground-truth temperatures obtained using a handheld infrared thermometer were used to calibrate the temperature obtained from the UAV thermal images and to evaluate the Tc extraction results. Measured leaf stomatal conductance values were used to evaluate the performance of the four Tc-based crop water stress indicators. The results showed a strong correlation between ground-truth Tc and Tc extracted by the red–green ratio index (RGRI)-Otsu method proposed in this study, with a coefficient of determination of 0.94 (n = 15) and root mean square error value of 0.7°C. The RGRI-Otsu method was most accurate for estimating temperatures around 32.9°C, but the magnitude of residuals increased above and below this value. This phenomenon may be attributable to changes in canopy cover (leaf curling) under water stress, resulting in changes in the proportion of exposed sunlit soil in UAV thermal orthophotographs. Therefore, to improve the accuracy of maize canopy detection and extraction, optimal methods and better strategies for eliminating mixed pixels are needed. This study demonstrates the potential of using high-resolution UAV RGB images to supplement UAV thermal images for the accurate extraction of maize Tc.

The rapid, accurate, and economical estimation of crop above-ground biomass at the farm scale is crucial for precision agricultural management. The unmanned aerial vehicle (UAV) remote-sensing system has a great application potential with the ability to obtain remote-sensing imagery with high temporal-spatial resolution. To verify the application potential of consumer-grade UAV RGB imagery in estimating maize above-ground biomass, vegetation indices and plant height derived from UAV RGB imagery were adopted. To obtain a more accurate observation, plant height was directly derived from UAV RGB point clouds. To search the optimal estimation method, the estimation performances of the models based on vegetation indices alone, based on plant height alone, and based on both vegetation indices and plant height were compared. The results showed that plant height directly derived from UAV RGB point clouds had a high correlation with ground-truth data with an R² value of 0.90 and an RMSE value of 0.12 m. The above-ground biomass exponential regression models based on plant height alone had higher correlations for both fresh and dry above-ground biomass with R² values of 0.77 and 0.76, respectively, compared to the linear regression model (both R² values were 0.59). The vegetation indices derived from UAV RGB imagery had great potential to estimate maize above-ground biomass with R² values ranging from 0.63 to 0.73. When estimating the above-ground biomass of maize by using multivariable linear regression based on vegetation indices, a higher correlation was obtained with an R² value of 0.82. There was no significant improvement of the estimation performance when plant height derived from UAV RGB imagery was added into the multivariable linear regression model based on vegetation indices. When estimating crop above-ground biomass based on UAV RGB remote-sensing system alone, looking for optimized vegetation indices and establishing estimation models with high performance based on advanced algorithms (e.g., machine learning technology) may be a better way.