Phenotyping has been a reality for aiding the selection of optimal crops for specific environments for decades in various horticultural industries. However, until recently, phenotyping was less accessible to tree breeders due to the size of the crop, the length of the rotation and the difficulty in acquiring detailed measurements. With the advent of affordable and non-destructive technologies, such as mobile laser scanners (MLS), phenotyping of mature forests is now becoming practical. Despite the potential of MLS technology, few studies included detailed assessments of its accuracy in mature plantations. In this study, we assessed a novel, high-density MLS operated below canopy for its ability to derive phenotypic measurements from mature Pinus radiata. MLS data were co-registered with above-canopy UAV laser scanner (ULS) data and imported to a pipeline that segments individual trees from the point cloud before extracting tree-level metrics. The metrics studied include tree height, diameter at breast height (DBH), stem volume and whorl characteristics. MLS-derived tree metrics were compared to field measurements and metrics derived from ULS alone. Our pipeline was able to segment individual trees with a success rate of 90.3%. We also observed strong agreement between field measurements and MLS-derived DBH (R² = 0.99, RMSE = 5.4%) and stem volume (R² = 0.99, RMSE = 10.16%). Additionally, we proposed a new variable height method for deriving DBH to avoid swelling, with an overall accuracy of 52% for identifying the correct method for where to take the diameter measurement. A key finding of this study was that MLS data acquired from below the canopy was able to derive canopy heights with a level of accuracy comparable to a high-end ULS scanner (R² = 0.94, RMSE = 3.02%), negating the need for capturing above-canopy data to obtain accurate canopy height models. Overall, the findings of this study demonstrate that even in mature forests, MLS technology holds strong potential for advancing forest phenotyping and tree measurement.

In commercial forestry and large-scale plant propagation, the utilization of artificial intelligence techniques for automated somatic embryo analysis has emerged as a highly valuable tool. Notably, image segmentation plays a key role in the automated assessment of mature somatic embryos. However, to date, the application of Convolutional Neural Networks (CNNs) for segmentation of mature somatic embryos remains unexplored. In this study, we present a novel application of CNNs for delineating mature somatic conifer embryos from background and residual proliferating embryogenic tissue and differentiating various morphological regions within the embryos. A semantic segmentation CNN was trained to assign pixels to cotyledon, hypocotyl, and background regions, while an instance segmentation network was trained to detect individual cotyledons for automated counting. The main dataset comprised 275 high-resolution microscopic images of mature Pinus radiata somatic embryos, with 42 images reserved for testing and validation sets. The evaluation of different segmentation methods revealed that semantic segmentation achieved the highest performance averaged across classes, achieving F1 scores of 0.929 and 0.932, with IoU scores of 0.867 and 0.872 for the cotyledon and hypocotyl regions respectively. The instance segmentation approach demonstrated proficiency in accurate detection and counting of the number of cotyledons, as indicated by a mean squared error (MSE) of 0.79 and mean absolute error (MAE) of 0.60. The findings highlight the efficacy of neural network-based methods in accurately segmenting somatic embryos and delineating individual morphological parts, providing additional information compared to previous segmentation techniques. This opens avenues for further analysis, including quantification of morphological characteristics in each region, enabling the identification of features of desirable embryos in large-scale production systems. These advancements contribute to the improvement of automated somatic embryogenesis systems, facilitating efficient and reliable plant propagation for commercial forestry applications.

The classification and quantification of fuel is traditionally a labour-intensive, costly and often subjective operation, especially in hazardous vegetation types, such as gorse (Ulex europaeus L.) scrub. In this study, unmanned aerial vehicle (UAV) technologies were assessed as an alternative to traditional field methodologies for fuel characterisation. UAV laser scanning (ULS) point clouds were captured, and a variety of spatial and intensity metrics were extracted from these data. These data were used as predictor variables in models describing destructively and non-destructively sampled field measurements of total above ground biomass (TAGB) and above ground available fuel (AGAF). Multiple regression of the structural predictor variables yielded correlations of R² = 0.89 and 0.87 for destructively sampled measurements of TAGB and AGAF, respectively, with relative root mean square error (RMSE) values of 18.6% and 11.3%, respectively. The best metrics for non-destructive field-measurements yielded correlations of R² = 0.50 and 0.49, with RMSE values of 40% and 30.8%, for predicting TAGB and AGAF, respectively, indicating that ULS-derived structural metrics offer higher levels of precision. UAV-derived versions of the field metrics (overstory height and cover) predicted TAGB and AGAF with R² = 0.44 and 0.41, respectively, and RMSE values of 34.5% and 21.7%, demonstrating that even simple metrics from a UAV can still generate moderate correlations. In further analyses, UAV photogrammetric data were captured and automatically processed using deep learning in order to classify vegetation into different fuel categories. The results yielded overall high levels of precision, recall and F1 score (0.83 for each), with minimum and maximum levels per class of F1 = 0.70 and 0.91. In conclusion, these ULS-derived metrics can be used to precisely estimate fuel type components and fuel load at fine spatial resolutions over moderate-sized areas, which will be useful for research, wildfire risk assessment and fuel management operations.

Samara morphology, including weight, size, and wing-to-seed ratios, is an important precursor to seed dispersal, and therefore a primary driver in large-scale alien conifer invasions. Prior studies have not reported morphological differences between samaras of different cones within a tree possibly because cone position at differing crown heights has not been examined. This preliminary study investigated whether cones from different crown heights of three lodgepole pine (Pinus contorta Douglas) trees differ in the morphological characteristics of their samaras. Samaras from the lower tree crown were 17% heavier on average than those from the upper crown, without any significant differences in wing loading. Cones in the upper crown produced more seeds than in the lower crown, although this was inconsistent across the small sample size. These results suggest the effects on primary seed-dispersal are negligible, but further research is needed to determine the effect on secondary seed-dispersal. Larger seeds from the lower crown are better adapted to survive in a competitive environment near other trees, while cones in the upper crown may produce more, but smaller, seeds which could infer a bet-hedging strategy when dispersing into heterogeneous environments. These results suggest canopy-height should be considered when accounting for inter-cone variation in conifers.