This study investigated the bactericidal effects of ultraviolet (UV) radiation, a high-voltage electric field (HVEF), and their combination on Escherichia coli. The results indicated that UV and combined disinfection were more effective with longer exposure, leading to significant reductions in microbial activity. Specifically, the single UV disinfection alone reduced activity by 3.3 log after 5 min, while combined disinfection achieved a 4.2 log reduction. In contrast, short-term HVEF treatment did not exhibit significant bactericidal effects, only achieving a reduction of 0.17 log in 5 min. Furthermore, prolonged exposure to both UV disinfection and an HVEF was found to damage cell membranes, ultimately causing cell death, while shorter durations did not. Despite rapid cell count decreases, flow cytometry did not detect apoptotic or necrotic cells, likely due to rapid cell rupture. This study suggests that combining UV radiation and an HVEF could be a promising approach for inhibiting bacterial reproduction, with HVEF enhancing UV effects. These findings provide insights for using combined HVEF and UV disinfection in food safety and preservation.

The application of a high-voltage electric field (HVEF) is crucial for mitigating the growth and reproduction of pathogenic microorganisms on solid surfaces. However, the electric field distribution of HVEF in the undischarged state and bactericidal effect remain largely unexplored. Herein, a unipolar wire–plate HVEF device was developed and its field strength distribution was simulated by COMOSL software (version 6.2). The simulation analysis revealed that the electric field strength and space charge density of HVEF in the undischarged state reached a maximum at the discharge electrode and a minimum at the middle of the two electrode wires. The electric field strength and the inhomogeneity of electric intensity distribution also decreased with the increase in electrode spacing. The sterilization effect of E. coli was positively correlated with voltage and treatment time, but negatively correlated with electrode spacing, which was consistent with the change in the field strength of the simulation results. When the voltage was −12.5 kv and electrode spacing 2.7 cm, the sterilization rate was 98.03% at 60 min. In addition, the increase in treatment temperature and relative humidity also affected the electric field distribution, thereby improving the bactericidal effect. Our results revealed that the electric field distribution was the main factor affecting the sterilization effect of HVEF, which provided theoretical support for the design of HVEF sterilization equipment and the optimization of sterilization performance

Haloalkane dehalogenase (DhaA) catalyzes the hydrolysis of halogenated compounds through the cleavage of carbon halogen bonds. However, the low activity, poor environmental stability, and difficult recycling of free DhaA greatly increases the economic cost of practical application. Inspired by the organic–inorganic hybrid system, an iron-based hybrid nanocomposite biocatalyst FeHN@DhaA is successfully constructed to enhance its environmental tolerability. A series of characterization methods demonstrate that the synthesized enzyme–metal iron complexes exhibit granular nanostructures with good crystallinity. Under optimized conditions, the activity recovery and the effective encapsulation yield of FeHN@DhaA are 138.54% and 87.21%, respectively. Moreover, it not only exhibits excellent immobilized enzymatic properties but also reveals better tolerance to extreme acid, and is alkali compared with the free DhaA. In addition, the immobilized enzyme FeHN@DhaA can be easily recovered and has a satisfactory reusability, retaining 57.8% of relative activity after five reaction cycles. The results of this study might present an alternative immobilized DhaA-based clean biotechnology for the decontamination of organochlorine pollutants.