The effects of dietary inclusion of dried Leucaena leucocephala leaves (DLL) on nutrient digestibility, fermentation parameters, microbial rumen population, and production of enteric methane (CH₄) in crossbred heifers were evaluated. Four heifers were used in a 4 × 4 Latin square design consisting of four periods and four levels of inclusion of DLL: 0%, 12%, 24%, and 36% of dry matter (DM) intake. Results showed that DM intake (DMI), organic matter intake, and gross energy intake (GEI) were similar (p > 0.05) among treatments. Apparent digestibility of organic matter, neutral detergent fiber, and energy decreased with increasing levels of DLL in the ration (p < 0.05). In contrast, digestible crude protein (CP) was higher (p < 0.05) in treatments with 12% and 24% DM of DLL. The inclusion of DLL did not affect (p > 0.05) rumen pH and total volatile fatty acids. Rumen microbial community was not affected (p > 0.05) by treatment. There was a linear reduction (p < 0.05) in CH₄ emissions as the levels of DLL in the ration were increased. Results of this study suggest that an inclusion of 12% DM of ration as DLL enhances digestible CP and reduces daily production of enteric CH₄ without adversely affecting DMI, rumen microbial population, and fermentation parameters.

Forage grass nutritional quality directly affects animal feed intake, productivity, and enteric methane (CH₄) emissions. This study evaluated the nutritional quality,in vitroenteric CH₄emission potential, and optimization of diets based on two widely grown tropical forage grasses either alone or mixed with legumes. The grassesUrochloahybrid cv. Cayman (UHC) andU. brizanthacv. Toledo (UBT), which typically have low concentrations of crude protein (CP), were incubatedin vitroeither alone or mixed with the legumesCanavalia brasiliensis(CB) andLeucaena diversifolia(LD), which have higher CP concentrations. Substitution of 30% of the grass dry matter (DM) with CB or LD did not affect gas production or DM degradability. After 96 h of incubation, accumulated CH₄was 87.3 mg CH₄g⁻¹DM and 107.7 mg CH₄g⁻¹DM for the grasses alone (UHC and UBT, respectively), and 100.7 mg CH₄g⁻¹DM and 113.2 mg CH₄g⁻¹DM for combined diets (70% grass, 15% CB, and 15% LD). Diets that combined legumes (CB or LC) and grass (UHC or UBT) had higher CP contents, gross, and metabolizable energy (GE, ME, respectively) densities, as well as lower concentrations of neutral detergent fiber (NDF) and acid detergent lignin (ADL). The ME and nutritional variables such as NFD, tannins (T), and CP showed a positive correlation within vitronet gas production, while ruminal digestibility was affected by CP, ADL, T, and GE. Optimal ratios of components for ruminant diets to reduce rumen net gas production and increase protein content were found with mixtures consisting of 60% grass (either UHC or UBT), 30% CB, and 10% LD. However, this ratio did not result in a decrease in CH₄production.

The present study aims to establish the relationship between chemical composition and in vitro methane (CH₄) production of high Andean grasses. For this purpose, eight species were collected in dry and rainy seasons: Alchemilla pinnata, Distichia muscoides, Carex ecuadorica, Hipochoeris taraxacoides, Mulhenbergia fastigiata, Mulhenbergia peruviana, Stipa brachiphylla and Stipa mucronata. They were chemically analyzed and incubated under an in vitro system. Species such as A. pinnata and H. taraxacoides were characterized by high crude protein (CP. 124 g/kg DM) and low neutral detergent fiber (NDF. 293 g/kg DM) contents in both seasons, contrary to Stipa grasses. This same pattern was obtained for H. taraxacoides, which presented the highest values of gas production, organic matter digestibility (DOM), metabolizable energy (ME) and CH₄ production (241 mL/g DM, 59% DOM, 8.4 MJ ME/kg DM and 37.7 mL CH₄/g DM, on average). For most species, the content of CP, acid detergent fiber (FDA) and ME was higher in the rainy season than in the dry season, which was the opposite for CH₄ production (p ≥ 0.05). In general, the nutritional content that most explained the behavior of CH₄ production was the NDF content (R² = 0.69). Grasses characterized by high NDF content produced less CH₄ (R = −0.85).

In the last decades, strategies have been evaluated to reduce rumen methane (CH₄) production by supplementing tropical forages rich in secondary compounds; however, most of these beneficial effects need to be validated in terms of their persistence over time. The aim of this study was to assess CH₄ emissions over time in heifers fed with and without Gliricidia sepium foliage (G) mixed with ground pods of Enterolobium cyclocarpum(E). Two groups of 4 crossbred (Bos taurus x Bos indicus) heifers (284 ±17 kg initial weight) were fed with 2 diets (0% and 15% of a mixture of the pods and foliage [E + G:0 and E + G:15, respectively]) over 80 d, plus 2 wk before the experiment, in which every animal was fed a legume and pod-free diet. Every 14 d, CH₄ production, apparent digestibility, volatile fatty acids (VFA), and microbial population were quantified for each animal. The experiment was conducted with a repeated measurements design over time. Diets fed differed in terms of their crude protein (CP), condensed tannins, and saponins content supplied by E. cyclocarpum and G. sepium. For most of the experiment, dry matter intake (DMI) and digestible dry-matter intake (DDMI) were 6.3 kg DMI/d and 512 g DDMI/kg, respectively, for both diets (diet: P > 0.05). Apparent digestible crude protein (DCP) was reduced by 21 g DCP/kg DM when the diet was supplemented with E + G:15 (P = 0.040). Molar proportions of VFA’s in the rumen did not differ between diets or in time (P > 0.05). Daily methane production, expressed in relation to DMI, was 23.95 vs. 23.32 g CH₄/kg DMI for the diet E + G:0 and E + G:15, respectively (diet: P = 0.016; Time: P > 0.05). Percent gross energy loss as CH₄ (Ym) with grass-only diets was above 8.1%, whereas when feeding heifers with the alternate supplementation, Ym values of 7.59% (P = 0.016) were observed. The relative abundance of total bacterial, protozoa, and methanogenic archaeal replicates was not affected by time nor by the incorporation of legume and pods into the diet (P > 0.05). Results suggest that addition of G. sepium mixed with E. cyclocarpum pods can reduce CH₄ production in heifers and this response remains over time, without effect on microbial population and VFA concentration and a slight reduction in CPD digestibility.

The aim of this research was to evaluate dietary inclusion of Leucaena diversifolia into a grass monoculture (GM) diet and its influence on enteric methane emissions, animal live weight gain and meat fatty acid content of steers. Two GM diets comprised Urochloa hybrid cv. Cayman or U. brizantha cv. Toledo; silvopastoral (SPS) mixed diets included an association of either grass with L. diversifolia in a 74:26 (grass:legume) planting ratio. The experiment was conducted over 16 months with 14 Brahman x Angus crossbred steers (374±7.5 kg body weight), divided into two diet groups. Steers were weighed periodically during the experiment, and methane emissions were quantified with the polytunnel technique. The meat quality and fatty acid profile were measured after slaughtering the steers. Energy density, total and digestible dry matter intake and crude protein were higher in steers fed SPS than GM (P≤0.05). At the end of the experiment, animals from SPS weighed on average 63 kg more than animals offered GM (535 vs. 472 kg; P=0.025). Net methane emitted by steers offered SPS was 142 g per day, this is equivalent to 15% less than emissions from animals that were offered GM (168 g; P=0.007). The meat fatty acid profile was similar between diets, except for cervoniv-DHA and omega-6: omega-3 ratio, which were higher in steers consuming GM diet (P≤0.05). The results of this study suggest that the inclusion of L. diversifolia improves nutrient intake, increases animal productivity, reduces daily enteric methane emission, and decreases the omega-6: omega-3 ratio in meat.

The objective of this research was to determine the effect of cassava (Manihot esculenta Crantz) supplementation on enteric methane (CH₄) emissions, carbon footprint, and production parameters in dairy cows. Daily concentrate supply for Jersey and Jersey * Holstein breeds was evaluated in four treatments (T): T1: 100% commercial concentrate; T2: 70% concentrate + 30% cassava leaves; T3: 70% concentrate + 30% cassava roots; and T4: 70% concentrate + 15% cassava leaves + 15% cassava root chips. Measurements of CH₄ emissions were performed using the polytunnel technique. Average daily dry matter intake ranged from 7.8 to 8.5 kg dry matter (DM). Cassava leaves were characterized by a high crude protein (CP) content (171 g CP/kg DM), with 5 times more neutral detergent fiber (NDF) content than cassava root (587 vs. 108 g NDF/kg DM). Average enteric CH₄ emissions per animal ranged from 194 to 234 g/d (p > 0.05). The carbon footprint was reduced by replacing 30% of the concentrate with cassava leaves and/or roots. Energy-corrected milk production was 1.15 times higher in Jersey * Holstein animals than Jersey cows (47 vs. 55 kg). Therefore, supplementation with cassava leaves and/or roots is a nutritionally and environmentally sustainable strategy.