To develop medical-grade stainless-steel 316L implants that are biocompatible, non-toxic and antibacterial, such implants need to be coated with biomaterials to meet the current demanding properties of biomedical materials. Hydroxyapatite (HA) is commonly used as a bone implant coating due to its excellent biocompatible properties. Zinc oxide (ZnO) nanoparticles are added to HA to increase its antibacterial and cohesion properties. The specimens were made of a stainless-steel grade 316 substrate coated with HA-ZnO using the electrophoretic deposition technique (EPD), and were subsequently characterized using scanning electron microscopy (SEM), energy dispersive X-ray (EDX), stylus profilometry, electrochemical corrosion testing and Fourier transform infrared (FTIR) spectroscopy. Additionally, cross-hatch tests, cell viability assays, antibacterial assessment and in vitro activity tests in simulated body fluid (SBF) were performed. The results showed that the HA-ZnO coating was uniform and resistant to corrosion in an acceptable range. FTIR confirmed the presence of HA-ZnO compositions, and the in vitro response and adhesion were in accordance with standard requirements for biomedical materials. Cell viability confirmed the viability of cells in an acceptable range (>70%). In addition, the antibacterial activity of ZnO was confirmed on Staphylococcus aureus. Thus, the HA-ZnO samples are recommended for biomedical applications.