There are a variety of amino acids and polysaccharides contained in Pleurotus eryngii that can improve immunity and prevent cancer in humans. The purpose of this research was to investigate the improved quality hypha of P. eryngii-3, which could shorten the ripening cycle of the hypha and increase economic benefits to industries. In this paper, the quality hypha of P. eryngii-3 was investigated for the DCW, total protein, polysaccharides, laccase activity, and so on. The medium was screened and carbon and nitrogen sources were screened and optimized as 40 g/L of corn flour and soybean meal by P. eryngii-3. Then, the effects of pH, temperature, volume, and rotation speed were investigated, in which the suitable fermentation conditions were, respectively, pH = 6, a temperature of 27 °C, 90 mL of fermentation broth, and a 150 r/min rotation speed. Meanwhile, the transcription levels of Lcc1, Lcc5, Lcc8, and Lcc12 were upregulated by 10.25-, 3.32-, 2.33-, and 6.29-fold, respectively, after the optimization. In addition, the response surface method of the BBD was utilized for further improvements to the DCW by P. eryngii-3, in which the optimized DCW reached 17.2345 ± 0.7845 g/L.

There were a variety of amino acids and polysaccharides in Pleurotus eryngii contained that could improve human immunity and prevent cancer. In this paper, the medium was screened and carton and nitrogen sources were screened and optimized as 40 g/L corn flour and soybean meal by P. eryngii-3. Then, the effect of pH, temperature, volume, and rotate speed were investigated, in which the suitable fermentation conditions were respectively pH = 6, 27 ℃, 90 mL of fermentation broth, and 150 r/min of rotate speed. Meanwhile, the transcription levels of lcc1, lcc5, lcc8, and lcc12 were up-regulated by 10.25-, 3.32-, 2.33- and 6.29-fold respectively after optimization. In addition, the box-behnken design was utilized to improve DCW (17.23 g/L). This research was optimized the medium and condition of fermentation by P. eryngii-3, which could shorten the ripening cycle of seeds and improve economic benefits for industries.