Surfactants are among the main chemical contaminants in greywater (GW) and can cause severe health issues in humans and aquatic organisms. We assessed the performance of a multistage constructed wetland system (EvaTAC) for GW treatment and capacity of the microbial community in linear alkyl benzene sulfonate (LAS) biodegradation. Physicochemical analyses were performed over 497 d, and biomass samples were collected for high-throughput DNA sequencing. The system was predominated by anaerobic conditions and received an average chemical oxygen demand (COD) and LAS of 374 and 32 mg·L⁻¹, with removal rates of 66% and 43%, respectively. A positive correlation between COD and LAS suggested COD as a design parameter for LAS removal. We identified microbial genera participating in hydrolysis, fermentation, syntrophy, acetogenesis, methanogenesis, surfactant degradation, and sulphate reduction. Among the 15 surfactant-degrading genera, Pseudomonas was predominant. Community richness and diversity indices were comparable between subsystems, with a slight decrease in diversity observed towards the outlet. Among the LAS degraders, Rhodopseudomonas palustris had the highest relative abundance of operational taxonomic unit (OTU)s in all samples and the highest richness in the anaerobic chamber. The patterns in microbial community composition and environmental conditions suggest that LAS biodegradation occurred throughout the EvaTAC system.

There is a worldwide range of technical sanitation guidelines focusing on small or traditional and isolated communities for ecological alternatives at the household level. However, a computational tool (software) that has a database and connects these guidelines in a single reference for resource-oriented sanitation concept decision making is still lacking. In this regard, an easy-to-use tool was developed using a participatory approach for the decision-making process from a choice of technical solutions to a type of system management. The results obtained from a pilot study indicate that the proposed tool in this paper will help with the decision-making process to aid in not only choosing sustainable sanitation solutions, but also sustainable operation and maintenance options for the systems. When presenting and discussing the tool with research groups and technicians, the potential for participatory application was noticed. The proposed tool can be used in the elaboration of municipal sanitation plans, assisting local technicians and environmental licensing agencies, designers and engineering students, among others. The software can be applied with other management tools, such as 5W2H and Canvas business model.

Greywater (GW) can be separated in different fractions where the kitchen component might be included. Constructed wetland (CW) systems are commonly used for the onsite treatment of GW, and the fraction treated might impact the performance, operation, and maintenance. These aspects are still poorly explored in the literature and are of importance for a proper design and system sustainability. In this study, a multi-stage household-scale CW system composed of a horizontal flow (HF), followed by a vertical flow (VF) unit, was monitored over 1330 days, focusing on different GW fractions and hydraulic and organic loading rates. The biochemical oxygen demand (BOD) was ~50% lower without the kitchen sink component (GW_L) in the system inlet, while no drop was observed in the chemical oxygen demand (COD). Treatment with the GW_L component caused a sudden drop in the hydraulic loading rate applied at the HF-CW (~114 to 35 mm per day) and the VF-CW (~230 to 70 mm per day). Even when the HF-CW received ~90 gCOD m⁻² per day (GW), the multistage system reached a COD removal of 90%. The lower BOD load when treating GW_L avoids clogging and decreases the frequency of maintenance. These variables can be used for the optimal design and operation of a CW, contributing with empirical data to CW guidelines in Brazil, and could additionally be expanded for application in other countries with similar climates.

Urban water management faces growing pressure from population growth, pollution, and climate variability, demanding innovative strategies to ensure long-term sustainability. This study applies the Life Cycle Sustainability Assessment (LCSA) across four case studies in Brazil and Germany, evaluating integrated systems that combine constructed wetlands for greywater treatment with rainwater harvesting for non-potable use. The scenarios include a single-family household, a high-rise residential building, a rural residence, and worker housing. A multi-criteria analysis was conducted to derive consolidated sustainability indicators, and sensitivity analysis explored the influence of dimension weighting. Results showed that water reuse scenarios consistently outperformed conventional counterparts across environmental, economic, and social dimensions. Life Cycle Assessment (LCA) revealed notable reductions in global warming potential, terrestrial acidification, and eutrophication. Life Cycle Costing (LCC) confirmed financial feasibility when externalities were considered, especially in large-scale systems. Social Life Cycle Assessment (S-LCA) highlighted the perceived benefits in terms of health, safety, and sustainability engagement. Integrated water reuse systems achieved overall sustainability scores up to 4.8 times higher than their baseline equivalents. These findings underscore the effectiveness of decentralized water reuse as a complementary and robust alternative to conventional supply and treatment models, supporting climate resilience and sustainable development goals.

This paper shows the influence of turbidity (in Nephelometric Turbidity Units - NTU), chemical oxygen demand (COD) and aeration (CO2 supply) on the productivity and growth rate and lipid content of microalgae (a mixed culture predominantly composed of Chlorella vulgaris), using anaerobically digested vinasse as a culture medium. The microalgae can be cultivated in anaerobically digested vinasse, at turbidity and chemical oxygen demand of 690 NTU and 2.5 gCOD L -1, respectively, according to the modified Gompertz model, and removal of turbidity by filtration did not influence the microalgae productivity (≈ 77 mg L1 d1). Furthermore, aeration increased the productivity up to 139 mg L1 d1, with a biomass dry weight of 2.7 g L-1. Finally, a maximum lipid content of 265 mg L -1 was obtained, while a nitrogen removal of 98% was recorded for all conditions. Thus, the combination of anaerobic digestion followed by the use of the digestate for the cultivation of microalgae may be an efficient way to treat large quantities of this residue, in turn yielding large amounts of microalgae biomass, which can be transformed into fertilizer and biofuel.