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Yueqin Wang
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

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Journal article
Published: 27 March 2020 in Insects
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Black cutworm (BCW), Agrotis ipsilon (Hufnagel), is an occasional pest of maize that can cause considerable economic loss and injury to corn seedlings. This research mainly assessed the susceptibility of BCW neonates to 11 Bt toxins (Cry1Ab, Cry1Ac, Cry1Ah, Cry1F, Cry1Ie, Cry1B, Cry2Aa, Vip3_ch1, Vip3_ch4, Vip3Ca2, Vip3Aa19) by exposing neonates to an artificial diet containing Bt toxins and evaluated the efficacy of three transgenic maize events (C008, C009, C010) expressing Vip3Aa19 toxin against BCW. The toxin-diet bioassay data indicated that Vip3Aa19 protein (LC50 = 0.43 μg/g) was the most active against BCW. Chimeric protein Vip3_ch1 (LC50 = 5.53 μg/g), Cry1F (LC50 = 83.62 μg/g) and Cry1Ac (LC50 = 184.77 μg/g) were less toxic. BCW was very tolerant to the other Bt toxins tested, with LC50 values more than 200 μg/g. Greenhouse studies were conducted with artificial infestations at the whorl stage by placing second-instar BCW larvae into whorl leaf and the fourth-instar larvae at the base of maize seedings. These results suggest that these transgenic maize events expressing Vip3Aa19 can provide effective control for BCW.

ACS Style

Xiaorui Yan; Junjiao Lu; Meifeng Ren; Yin He; Yueqin Wang; Zhenying Wang; Kanglai He. Insecticidal Activity of 11 Bt toxins and 3 Transgenic Maize Events Expressing Vip3Aa19 to Black Cutworm, Agrotis ipsilon (Hufnagel). Insects 2020, 11, 208 .

AMA Style

Xiaorui Yan, Junjiao Lu, Meifeng Ren, Yin He, Yueqin Wang, Zhenying Wang, Kanglai He. Insecticidal Activity of 11 Bt toxins and 3 Transgenic Maize Events Expressing Vip3Aa19 to Black Cutworm, Agrotis ipsilon (Hufnagel). Insects. 2020; 11 (4):208.

Chicago/Turabian Style

Xiaorui Yan; Junjiao Lu; Meifeng Ren; Yin He; Yueqin Wang; Zhenying Wang; Kanglai He. 2020. "Insecticidal Activity of 11 Bt toxins and 3 Transgenic Maize Events Expressing Vip3Aa19 to Black Cutworm, Agrotis ipsilon (Hufnagel)." Insects 11, no. 4: 208.

Journal article
Published: 04 February 2020 in Toxins
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The bacterium Bacillus thuringiensis produces insecticidal Vip3 proteins during the vegetative growth phase with activity against several lepidopteran pests. To date, three different Vip3 protein families have been identified based on sequence identity: Vip3A, Vip3B, and Vip3C. In this study, we report the construction of chimeras by exchanging domains between Vip3Aa and Vip3Ca, two proteins with marked specificity differences against lepidopteran pests. We found that some domain combinations made proteins insoluble or prone to degradation by trypsin as most abundant insect gut protease. The soluble and trypsin-stable chimeras, along with the parental proteins Vip3Aa and Vip3Ca, were tested against lepidopteran pests from different continents: Spodoptera exigua, Spodoptera littoralis, Spodoptera frugiperda, Helicoverpa armigera, Mamestra brassicae, Anticarsia gemmatalis, and Ostrinia furnacalis. The exchange of the Nt domain (188 N-terminal amino acids) had little effect on the stability and toxicity (equal or slightly lower) of the resulting chimeric protein against all insects except for S. frugiperda, for which the chimera with the Nt domain from Vip3Aa and the rest of the protein from Vip3Ca showed a significant increase in toxicity compared to the parental Vip3Ca. Chimeras with the C-terminal domain from Vip3Aa (from amino acid 510 of Vip3Aa to the Ct) with the central domain of Vip3Ca (amino acids 189–509 based on the Vip3Aa sequence) made proteins that could not be solubilized. Finally, the chimera including the Ct domain of Vip3Ca and the Nt and central domain from Vip3Aa was unstable. Importantly, an insect species tolerant to Vip3Aa but susceptible to Vip3Ca, such as Ostrinia furnacalis, was also susceptible to chimeras maintaining the Ct domain from Vip3Ca, in agreement with the hypothesis that the Ct region of the protein is the one conferring specificity to Vip3 proteins.

ACS Style

Joaquín Gomis-Cebolla; Rafael Ferreira dos Santos; Yueqin Wang; Javier Caballero; Primitivo Caballero; Kanglai He; Juan Jurat-Fuentes; Juan Ferré. Domain Shuffling between Vip3Aa and Vip3Ca: Chimera Stability and Insecticidal Activity against European, American, African, and Asian Pests. Toxins 2020, 12, 99 .

AMA Style

Joaquín Gomis-Cebolla, Rafael Ferreira dos Santos, Yueqin Wang, Javier Caballero, Primitivo Caballero, Kanglai He, Juan Jurat-Fuentes, Juan Ferré. Domain Shuffling between Vip3Aa and Vip3Ca: Chimera Stability and Insecticidal Activity against European, American, African, and Asian Pests. Toxins. 2020; 12 (2):99.

Chicago/Turabian Style

Joaquín Gomis-Cebolla; Rafael Ferreira dos Santos; Yueqin Wang; Javier Caballero; Primitivo Caballero; Kanglai He; Juan Jurat-Fuentes; Juan Ferré. 2020. "Domain Shuffling between Vip3Aa and Vip3Ca: Chimera Stability and Insecticidal Activity against European, American, African, and Asian Pests." Toxins 12, no. 2: 99.

Journal article
Published: 06 August 2019 in Toxins
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Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) have revolutionized pest control, but the benefits of this approach have been reduced by the evolution of resistance in pests. The widely adopted 'pyramid strategy' for delaying resistance entails transgenic crops producing two or more distinct toxins that kill the same pest. The limited experimental evidence supporting this strategy comes primarily from a model system under ideal conditions. Here we tested the pyramid strategy under nearly worst-case conditions, including some cross-resistance between the toxins in the pyramid. In a laboratory selection experiment with an artificial diet, we used Bt toxins Cry1Ab, Cry1F, and Cry1Ie singly or in pairs against Ostrinia furnacalis, one of the most destructive pests of corn in Asia. Under the conditions evaluated, pairs of toxins did not consistently delay the evolution of resistance relative to single toxins.

ACS Style

Yueqin Wang; Yudong Quan; Jing Yang; Changlong Shu; Zhenying Wang; Jie Zhang; Angharad M. R. Gatehouse; Bruce E. Tabashnik; Kanglai He. Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs. Toxins 2019, 11, 461 .

AMA Style

Yueqin Wang, Yudong Quan, Jing Yang, Changlong Shu, Zhenying Wang, Jie Zhang, Angharad M. R. Gatehouse, Bruce E. Tabashnik, Kanglai He. Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs. Toxins. 2019; 11 (8):461.

Chicago/Turabian Style

Yueqin Wang; Yudong Quan; Jing Yang; Changlong Shu; Zhenying Wang; Jie Zhang; Angharad M. R. Gatehouse; Bruce E. Tabashnik; Kanglai He. 2019. "Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs." Toxins 11, no. 8: 461.

Journal article
Published: 07 June 2017 in Toxins
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A strain of the Asian corn borer (ACB), Ostrinia furnacalis (Guenée), has evolved >800-fold resistance to Cry1Ie (ACB-IeR) after 49 generations of selection. The inheritance pattern of resistance to Cry1Ie in ACB-IeR strain and its cross-resistance to other Bt toxins were determined through bioassay by exposing neonates from genetic-crosses to toxins incorporated into the diet. The response of progenies from reciprocal F1 crosses were similar (LC50s: 76.07 vs. 74.32 μg/g), which suggested the resistance was autosomal. The effective dominance (h) decreased as concentration of Cry1Ie increased. h was nearly recessive or incompletely recessive on Cry1Ie maize leaf tissue (h = 0.02), but nearly dominant or incompletely dominant (h = 0.98) on Cry1Ie maize silk. Bioassay of the backcross suggested that the resistance was controlled by more than one locus. In addition, the resistant strain did not perform cross-resistance to Cry1Ab (0.8-fold), Cry1Ac (0.8-fold), Cry1F (0.9-fold), and Cry1Ah (1.0-fold). The present study not only offers the manifestation for resistance management, but also recommends that Cry1Ie will be an appropriate candidate for expression with Cry1Ab, Cry1Ac, Cry1F, or Cry1Ah for the development of Bt maize.

ACS Style

Yueqin Wang; Jing Yang; Yudong Quan; Zhenying Wang; Wanzhi Cai; Baltasar Escriche. Characterization of Asian Corn Borer Resistance to Bt Toxin Cry1Ie. Toxins 2017, 9, 186 .

AMA Style

Yueqin Wang, Jing Yang, Yudong Quan, Zhenying Wang, Wanzhi Cai, Baltasar Escriche. Characterization of Asian Corn Borer Resistance to Bt Toxin Cry1Ie. Toxins. 2017; 9 (6):186.

Chicago/Turabian Style

Yueqin Wang; Jing Yang; Yudong Quan; Zhenying Wang; Wanzhi Cai; Baltasar Escriche. 2017. "Characterization of Asian Corn Borer Resistance to Bt Toxin Cry1Ie." Toxins 9, no. 6: 186.