This page has only limited features, please log in for full access.
Laboratory selection for resistance of field populations is a well-known and useful tool to understand the potential of insect populations to evolve resistance to insecticides. It provides us with estimates of the frequency of resistance alleles and allows us to study the mechanisms by which insects developed resistance to shed light on the mode of action and optimize resistance management strategies. Here, a field population of Mythimna separata was subjected to laboratory selection with either Vip3Aa, Cry1Ab, or Cry1F insecticidal proteins from Bacillus thuringiensis. The population rapidly evolved resistance to Vip3Aa reaching, after eight generations, a level of >3061-fold resistance, compared with the unselected insects. In contrast, the same population did not respond to selection with Cry1Ab or Cry1F. The Vip3Aa resistant population did not show cross resistance to either Cry1Ab or Cry1F. Radiolabeled Vip3Aa was tested for binding to brush border membrane vesicles from larvae from the susceptible and resistant insects. The results did not show any qualitative or quantitative difference between both insect samples. Our data, along with previous results obtained with other Vip3Aa-resistant populations from other insect species, suggest that altered binding to midgut membrane receptors is not the main mechanism of resistance to Vip3Aa.
Yudong Quan; Jing Yang; Yueqin Wang; Patricia Hernández-Martínez; Juan Ferré; Kanglai He. The Rapid Evolution of Resistance to Vip3Aa Insecticidal Protein in Mythimna separata (Walker) Is Not Related to Altered Binding to Midgut Receptors. Toxins 2021, 13, 364 .
AMA StyleYudong Quan, Jing Yang, Yueqin Wang, Patricia Hernández-Martínez, Juan Ferré, Kanglai He. The Rapid Evolution of Resistance to Vip3Aa Insecticidal Protein in Mythimna separata (Walker) Is Not Related to Altered Binding to Midgut Receptors. Toxins. 2021; 13 (5):364.
Chicago/Turabian StyleYudong Quan; Jing Yang; Yueqin Wang; Patricia Hernández-Martínez; Juan Ferré; Kanglai He. 2021. "The Rapid Evolution of Resistance to Vip3Aa Insecticidal Protein in Mythimna separata (Walker) Is Not Related to Altered Binding to Midgut Receptors." Toxins 13, no. 5: 364.
Yellow Peach Moth (YPM), Conogethes punctiferalis (Guenée), is one of the most destructive maize pests in the Huang-Huai-Hai summer maize region of China. Transgenic Bacillus thuringiensis (Bt) maize provides an effective means to control this insect pest in field trials. However, the establishment of Bt resistance to target pests is endangering the continued success of Bt crops. To use Bt maize against YPM, the baseline susceptibility of the local populations in the targeted areas needs to be verified. Diet-overlay bioassay results showed that all the fourteen YPM populations in China are highly susceptible to Cry1Ab. The LC50 values ranged from 0.35 to 2.38 ng/cm2 over the two years of the collection, and the difference between the most susceptible and most tolerant populations was sevenfold. The upper limit of the LC99 estimates of six pooled populations produced >99% larval mortality for representative eight populations collected in 2020 and was designated as diagnostic concentrations for monitoring susceptibility in YPM populations in China. Hence, we evaluated the laboratory selection of resistance in YPM to Cry1Ab using the diet-overlay bioassay method. Although the resistant ratio was generally low, YPM potentially could evolve resistance to Cry1Ab. The potential developmentof resistance by target pests points out the necessity to implement resistance management strategies for delaying the establishment of pest resistance to Bt crops.
Su Shwe; Sivaprasath Prabu; Yu Chen; Qincheng Li; Dapeng Jing; Shuxiong Bai; Kanglai He; Zhenying Wang. Baseline Susceptibility and Laboratory Selection of Resistance to Bt Cry1Ab Protein of Chinese Populations of Yellow Peach Moth, Conogethes punctiferalis (Guenée). Toxins 2021, 13, 335 .
AMA StyleSu Shwe, Sivaprasath Prabu, Yu Chen, Qincheng Li, Dapeng Jing, Shuxiong Bai, Kanglai He, Zhenying Wang. Baseline Susceptibility and Laboratory Selection of Resistance to Bt Cry1Ab Protein of Chinese Populations of Yellow Peach Moth, Conogethes punctiferalis (Guenée). Toxins. 2021; 13 (5):335.
Chicago/Turabian StyleSu Shwe; Sivaprasath Prabu; Yu Chen; Qincheng Li; Dapeng Jing; Shuxiong Bai; Kanglai He; Zhenying Wang. 2021. "Baseline Susceptibility and Laboratory Selection of Resistance to Bt Cry1Ab Protein of Chinese Populations of Yellow Peach Moth, Conogethes punctiferalis (Guenée)." Toxins 13, no. 5: 335.
In the Asian corn borer (ACB), Ostrinia furnacalis (Guenée), diapause is governed by a multigenetic constitution that responds to daylength and temperature with seasonality. The ACB displays uni- or multivoltinism, depending on its geographic specificity. Hence, warmer temperatures may result in alternation of voltinism in the ACB, which will help in understanding the ecological consequences of climate warming on insects. In the present study, we investigated the voltinism in two natural populations from Harbin (H) and Gongzhuling (G) as well as a laboratory (L) population (established from the H population in 2017) of the ACB, at ambient and elevated atmospheric CO2 (aCO2 390 μL/L and eCO2 750 μL/L) and temperature (aT and Et = At + 2 °C). From the diapause response, both the uni- and multivoltine ecotypes were coexisting in the H population. The neonate occurrence date of 50% individuals that induced diapause was ca. 10 days later in the G population than in the H population, but it was about 10 days earlier than in the L population. Comparing to the dates of onset and the peak of diapause induction, the G and L populations were less variable than the H population in response to a short and/or shortening daylength in the field. The univoltine individuals could not be eliminated completely after 19 generations of selection. Diapause incidence decreased with a climate-warming scenario, which was temporally specific and could be overridden by significantly low daily average temperatures. The eCO2 did not directly impact the voltinism. On the basis of voltinism, the H population was sympatric for uni- and multivoltine ecotypes, with multivoltinism being dominant. The univoltinism trait was recessive. Climate warming could significantly override the effect of photoperiod, which was yearly dependent. Warmer temperatures and a decreased latitude (shortened daylength), and their interaction, would drive ACB evolution toward diapause homogeneity for multivoltinism.
Kai-Qiang Liu; Lian-Xia Wang; Tian-Tao Zhang; Shu-Xiong Bai; Ke-Qin Wang; Zhen-Ying Wang; Kang-Lai He; William Hutchison. Voltine Ecotypes of the Asian Corn Borer and Their Response to Climate Warming. Insects 2021, 12, 232 .
AMA StyleKai-Qiang Liu, Lian-Xia Wang, Tian-Tao Zhang, Shu-Xiong Bai, Ke-Qin Wang, Zhen-Ying Wang, Kang-Lai He, William Hutchison. Voltine Ecotypes of the Asian Corn Borer and Their Response to Climate Warming. Insects. 2021; 12 (3):232.
Chicago/Turabian StyleKai-Qiang Liu; Lian-Xia Wang; Tian-Tao Zhang; Shu-Xiong Bai; Ke-Qin Wang; Zhen-Ying Wang; Kang-Lai He; William Hutchison. 2021. "Voltine Ecotypes of the Asian Corn Borer and Their Response to Climate Warming." Insects 12, no. 3: 232.
Yellow peach moth, Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae), is a very destructive fruit borer in Eastern Asia and Australia. A successful artificial diet and rearing protocol are urgently needed for its biological control and research. In this study, we developed and assessed four artificial diets: AD‐1, AD‐2, AD‐3 and AD‐4, for rearing yellow peach moth larvae. AD‐1 is effective supplements, leading to the larval survival rate of 82.05%, percentage of pupation 79.48%, mean pupa weight of 76.53 mg, emergence rate of 95.25%, and an adult fecundity rate of 121 eggs/female. Using it to feed can shorten the whole generation developmental period to 33 days. Based on this diet AD‐1, we also develop a suitable mass rearing method. During the larval stages providing three‐ to four‐time fresh diet can keep a healthy larval colony.
Dapeng Jing; Tiantao Zhang; Shuxiong Bai; Kanglai He; Sivaprasath Prabu; Zhenying Wang. Artificial diet development for mass rearing and its effect on the reproduction of yellow peach moth, Conogethes punctiferalis (Guenée). Entomological Research 2021, 51, 127 -132.
AMA StyleDapeng Jing, Tiantao Zhang, Shuxiong Bai, Kanglai He, Sivaprasath Prabu, Zhenying Wang. Artificial diet development for mass rearing and its effect on the reproduction of yellow peach moth, Conogethes punctiferalis (Guenée). Entomological Research. 2021; 51 (3):127-132.
Chicago/Turabian StyleDapeng Jing; Tiantao Zhang; Shuxiong Bai; Kanglai He; Sivaprasath Prabu; Zhenying Wang. 2021. "Artificial diet development for mass rearing and its effect on the reproduction of yellow peach moth, Conogethes punctiferalis (Guenée)." Entomological Research 51, no. 3: 127-132.
1. Climate change is expected to increase the frequency and duration of extreme heat events, which would pose significant challenges to most ectotherm species, such as insects. 2. In the study, we address the thermal tolerance of corn leaf aphid, Rhopalosiphummaidis (Hemiptera: Aphididae) under brief extreme high‐temperature events (i.e. heat waves) across life stages. Immature instars and adults of R. maidis were exposed to heat events of increasing temperatures (0.5 °C min−1), starting at 22 °C and reaching one of the selected maximal temperatures (from 38.5 to 44.5 °C with an increased interval of 0.5 °C). In each temperature regime, the mortality of R. maidis was compared with and without barley seedlings. 3. Results showed that the critical high temperature (CHT) of R. maidis was 39.0 °C in no feeding treatments, while it was 39.5 °C in feeding treatments. The upper lethal temperatures (ULTs) of R. maidis were significantly different between feeding and no feeding treatments. In addition, the ULTs varied significantly across life stages with the highest ULT values for 4th instars. Feeding significantly increased the thermal tolerance of phytophagous insects. 4. Variable balances in plant‐herbivore interactions will be induced according to the insect feeding diet status and instar stage when experiencing extreme temperatures resulting from the warming climate. The findings will provide valuable information for understanding the role of food intake in thermal adaptation of small arthropods to extreme high‐temperature events under climate change.
Yu Chen; Yudong Quan; François Verheggen; Zhenying Wang; Frederic Francis; Kanglai He. Differential thermal tolerance across life stages under extreme high temperatures crossed with feeding status in corn leaf aphid. Ecological Entomology 2020, 46, 533 -540.
AMA StyleYu Chen, Yudong Quan, François Verheggen, Zhenying Wang, Frederic Francis, Kanglai He. Differential thermal tolerance across life stages under extreme high temperatures crossed with feeding status in corn leaf aphid. Ecological Entomology. 2020; 46 (3):533-540.
Chicago/Turabian StyleYu Chen; Yudong Quan; François Verheggen; Zhenying Wang; Frederic Francis; Kanglai He. 2020. "Differential thermal tolerance across life stages under extreme high temperatures crossed with feeding status in corn leaf aphid." Ecological Entomology 46, no. 3: 533-540.
Ostrinia furnacalis, is the major pest of maize causing significant yield losses. So far, many approaches have been used to increase the virulence of entomopathogenic fungal isolates. The current study is an attempt to estimate synergistic effect of Beauveria bassiana and Trichoderma asperellum in order to explore larval immune response through RNA sequencing and differentially expression analysis. In vivo synergism was examined in seven proportions (B. bassiana: T. asperellum = 1:1, 1:2, 1:3, 1:4, 4:1, 3:1, 2:1) and in the in vitro case, two inoculation methods were applied: seed coating and soil drenching. Results revealed significant decrease in plant damage and high larval mortality in fungal treatments. Fungal isolates mediated the plant defense by increasing proline, superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) and protease activities. Seed coating method was proved to be the most effective in case of maize endophytic colonization. In total, 59 immune-related differentially expressed genes DEGs were identified including, cytochrome P450, heat shock protein, ABC transporter, cadherin, peptidoglycan recognition protein (PGRP), cuticlular protein, etc. Further, transcriptomic response was confirmed by qRT-PCR. Our results concluded that, coculture of B. bassiana and T. asperellum has the synergistic potential to suppress the immune response of O. furnacalis and can be used as sustainable approach to induce plant resistance through activation of defense-related enzymes.
RaufA Batool; Muhammad Umer; Yangzhou Wang; Kanglai He; Tiantao Zhang; Shuxiong Bai; Yang Zhi; Jie Chen; Zhenying Wang. Synergistic Effect of Beauveria bassiana and Trichoderma asperellum to Induce Maize (Zea mays L.) Defense against the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera, Crambidae) and Larval Immune Response. International Journal of Molecular Sciences 2020, 21, 8215 .
AMA StyleRaufA Batool, Muhammad Umer, Yangzhou Wang, Kanglai He, Tiantao Zhang, Shuxiong Bai, Yang Zhi, Jie Chen, Zhenying Wang. Synergistic Effect of Beauveria bassiana and Trichoderma asperellum to Induce Maize (Zea mays L.) Defense against the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera, Crambidae) and Larval Immune Response. International Journal of Molecular Sciences. 2020; 21 (21):8215.
Chicago/Turabian StyleRaufA Batool; Muhammad Umer; Yangzhou Wang; Kanglai He; Tiantao Zhang; Shuxiong Bai; Yang Zhi; Jie Chen; Zhenying Wang. 2020. "Synergistic Effect of Beauveria bassiana and Trichoderma asperellum to Induce Maize (Zea mays L.) Defense against the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera, Crambidae) and Larval Immune Response." International Journal of Molecular Sciences 21, no. 21: 8215.
Evolution of insect resistance to Bt toxins challenges the use of Cry toxins to control agricultural pests. In lepidopterans, Cry toxin affinity towards multiple midgut epithelial receptors has become a matter of dispute. Cry1Ah toxin-binding proteins were identified in the larval midgut of susceptible (ACB-BtS) and resistant (ACB-AhR) strains of the Asian corn borer (ACB). A pull-down assay was performed using biotinylated Cry1Ah toxin, and the binding proteins were identified by employing liquid chromatography–tandem mass spectrometry (LC-MS/MS). This study aimed to find the binding consistency of the midgut epithelial protein to the Cry1Ah toxin. The binding proteins from different fractions of SDS-PAGE showed a different pattern. We observed an isoform of prophenoloxidase PPO1b (UniProt Acc No. A0A1Q1MKI0), which was found only in the ACB-AhR fractions. Prophenoloxidase (proPO) is an extraordinary defense molecule activated in insect species during pathogen invasion and the wound healing process. Importantly, this prophenoloxidase might have direct/indirect interaction with the Cry1Ah toxin. Our data also suggest that factors like techniques, enrichment of binding proteins in the sample and the reversible and irreversible nature of the brush border membrane vesicles (BBMVs) to Cry toxins could cause the inconsistency in the protein–protein interactions. Moreover, inside the larva midgut, the influence of the Cry toxins under physiological conditions might be different from the laboratory procedures.
Sivaprasath Prabu; Muhammad Shabbir; Zhenying Wang; Kanglai He. Analysis of Cry1Ah Toxin-Binding Reliability to Midgut Membrane Proteins of the Asian Corn Borer. Toxins 2020, 12, 418 .
AMA StyleSivaprasath Prabu, Muhammad Shabbir, Zhenying Wang, Kanglai He. Analysis of Cry1Ah Toxin-Binding Reliability to Midgut Membrane Proteins of the Asian Corn Borer. Toxins. 2020; 12 (6):418.
Chicago/Turabian StyleSivaprasath Prabu; Muhammad Shabbir; Zhenying Wang; Kanglai He. 2020. "Analysis of Cry1Ah Toxin-Binding Reliability to Midgut Membrane Proteins of the Asian Corn Borer." Toxins 12, no. 6: 418.
Bacillus thuringiensis (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 in vitro and could therefore be expressed in the alkaline gut of lepidopteron larvae. A ∆cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards Ostrinia furnacalis neonate larvae, indicating its important role in infection. Purified recombinant Escherichia coli CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP–CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter PcbpA was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible in vivo gut activity, last instar Galleria mellonella (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the ∆cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions.
Jiaxin Qin; Zongxing Tong; Yiling Zhan; Christophe Buisson; Fuping Song; Kanglai He; Christina Nielsen-Leroux; Shuyuan Guo. A Bacillus thuringiensis Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process. Toxins 2020, 12, 252 .
AMA StyleJiaxin Qin, Zongxing Tong, Yiling Zhan, Christophe Buisson, Fuping Song, Kanglai He, Christina Nielsen-Leroux, Shuyuan Guo. A Bacillus thuringiensis Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process. Toxins. 2020; 12 (4):252.
Chicago/Turabian StyleJiaxin Qin; Zongxing Tong; Yiling Zhan; Christophe Buisson; Fuping Song; Kanglai He; Christina Nielsen-Leroux; Shuyuan Guo. 2020. "A Bacillus thuringiensis Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process." Toxins 12, no. 4: 252.
Black cutworm (BCW), Agrotis ipsilon (Hufnagel), is an occasional pest of maize that can cause considerable economic loss and injury to corn seedlings. This research mainly assessed the susceptibility of BCW neonates to 11 Bt toxins (Cry1Ab, Cry1Ac, Cry1Ah, Cry1F, Cry1Ie, Cry1B, Cry2Aa, Vip3_ch1, Vip3_ch4, Vip3Ca2, Vip3Aa19) by exposing neonates to an artificial diet containing Bt toxins and evaluated the efficacy of three transgenic maize events (C008, C009, C010) expressing Vip3Aa19 toxin against BCW. The toxin-diet bioassay data indicated that Vip3Aa19 protein (LC50 = 0.43 μg/g) was the most active against BCW. Chimeric protein Vip3_ch1 (LC50 = 5.53 μg/g), Cry1F (LC50 = 83.62 μg/g) and Cry1Ac (LC50 = 184.77 μg/g) were less toxic. BCW was very tolerant to the other Bt toxins tested, with LC50 values more than 200 μg/g. Greenhouse studies were conducted with artificial infestations at the whorl stage by placing second-instar BCW larvae into whorl leaf and the fourth-instar larvae at the base of maize seedings. These results suggest that these transgenic maize events expressing Vip3Aa19 can provide effective control for BCW.
Xiaorui Yan; Junjiao Lu; Meifeng Ren; Yin He; Yueqin Wang; Zhenying Wang; Kanglai He. Insecticidal Activity of 11 Bt toxins and 3 Transgenic Maize Events Expressing Vip3Aa19 to Black Cutworm, Agrotis ipsilon (Hufnagel). Insects 2020, 11, 208 .
AMA StyleXiaorui Yan, Junjiao Lu, Meifeng Ren, Yin He, Yueqin Wang, Zhenying Wang, Kanglai He. Insecticidal Activity of 11 Bt toxins and 3 Transgenic Maize Events Expressing Vip3Aa19 to Black Cutworm, Agrotis ipsilon (Hufnagel). Insects. 2020; 11 (4):208.
Chicago/Turabian StyleXiaorui Yan; Junjiao Lu; Meifeng Ren; Yin He; Yueqin Wang; Zhenying Wang; Kanglai He. 2020. "Insecticidal Activity of 11 Bt toxins and 3 Transgenic Maize Events Expressing Vip3Aa19 to Black Cutworm, Agrotis ipsilon (Hufnagel)." Insects 11, no. 4: 208.
Background Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Results Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues. Conclusions Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be supportive for pest management studies in future.
Dapeng Jing; Tiantao Zhang; Shuxiong Bai; Kanglai He; Sivaprasath Prabu; Junbo Luan; Zhenying Wang. Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae). BMC Genomics 2020, 21, 244 -13.
AMA StyleDapeng Jing, Tiantao Zhang, Shuxiong Bai, Kanglai He, Sivaprasath Prabu, Junbo Luan, Zhenying Wang. Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae). BMC Genomics. 2020; 21 (1):244-13.
Chicago/Turabian StyleDapeng Jing; Tiantao Zhang; Shuxiong Bai; Kanglai He; Sivaprasath Prabu; Junbo Luan; Zhenying Wang. 2020. "Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)." BMC Genomics 21, no. 1: 244-13.
Phenoloxidase (PO) is a crucial enzyme in the Arthropods melanization process, in which synthesized melanin rapidly acts at the site of injury and infection. In this study, we observed significant changes in humoral and cellular responses after exposing susceptible and resistant strains to a sub-lethal concentration of Cry1Ah toxin. Based on STRING v 11.0 computational protein-protein interaction analysis, we selected seven immune genes namely Prophenoloxidase PPO1b, PP03, Serpin-3, Serpin-5, Beta-1,3-glucan recognition protein, Immulectin-3 and Serine protease SP105 reported in Asian corn borer. Quantitative real-time PCR gene expression studies showed Cry1Ah resistant strain had higher expression of PPO1b, PP03, Serpin-3, Beta-1,3-glucan recognition protein, Immulectin-3 and Serine protease SP105 genes in midgut and hemocyte samples. This study also investigated and found that the level of prophenoloxidation (proPO) activity in Cry1Ah resistant strains was significantly higher than susceptible strains. Cry1Ah toxin significantly increased the resistant strain's immune responses, the difference was observed through assays of bacterial agglutination and phagocytosis. Additionally, immune response induced by Cry1Ah toxin influences the microbiome composition associated with the host system. These parameters seem to explain the contribution of PO/PO regulating proteins render the host to resist the Cry1Ah toxin.
Sivaprasath Prabu; Dapeng Jing; Muhammad Zeeshan Shabbir; Weining Yuan; Zhenying Wang; Kanglai He. Contribution of phenoloxidase activation mechanism to Bt insecticidal protein resistance in Asian corn borer. International Journal of Biological Macromolecules 2020, 153, 88 -99.
AMA StyleSivaprasath Prabu, Dapeng Jing, Muhammad Zeeshan Shabbir, Weining Yuan, Zhenying Wang, Kanglai He. Contribution of phenoloxidase activation mechanism to Bt insecticidal protein resistance in Asian corn borer. International Journal of Biological Macromolecules. 2020; 153 ():88-99.
Chicago/Turabian StyleSivaprasath Prabu; Dapeng Jing; Muhammad Zeeshan Shabbir; Weining Yuan; Zhenying Wang; Kanglai He. 2020. "Contribution of phenoloxidase activation mechanism to Bt insecticidal protein resistance in Asian corn borer." International Journal of Biological Macromolecules 153, no. : 88-99.
The bacterium Bacillus thuringiensis produces insecticidal Vip3 proteins during the vegetative growth phase with activity against several lepidopteran pests. To date, three different Vip3 protein families have been identified based on sequence identity: Vip3A, Vip3B, and Vip3C. In this study, we report the construction of chimeras by exchanging domains between Vip3Aa and Vip3Ca, two proteins with marked specificity differences against lepidopteran pests. We found that some domain combinations made proteins insoluble or prone to degradation by trypsin as most abundant insect gut protease. The soluble and trypsin-stable chimeras, along with the parental proteins Vip3Aa and Vip3Ca, were tested against lepidopteran pests from different continents: Spodoptera exigua, Spodoptera littoralis, Spodoptera frugiperda, Helicoverpa armigera, Mamestra brassicae, Anticarsia gemmatalis, and Ostrinia furnacalis. The exchange of the Nt domain (188 N-terminal amino acids) had little effect on the stability and toxicity (equal or slightly lower) of the resulting chimeric protein against all insects except for S. frugiperda, for which the chimera with the Nt domain from Vip3Aa and the rest of the protein from Vip3Ca showed a significant increase in toxicity compared to the parental Vip3Ca. Chimeras with the C-terminal domain from Vip3Aa (from amino acid 510 of Vip3Aa to the Ct) with the central domain of Vip3Ca (amino acids 189–509 based on the Vip3Aa sequence) made proteins that could not be solubilized. Finally, the chimera including the Ct domain of Vip3Ca and the Nt and central domain from Vip3Aa was unstable. Importantly, an insect species tolerant to Vip3Aa but susceptible to Vip3Ca, such as Ostrinia furnacalis, was also susceptible to chimeras maintaining the Ct domain from Vip3Ca, in agreement with the hypothesis that the Ct region of the protein is the one conferring specificity to Vip3 proteins.
Joaquín Gomis-Cebolla; Rafael Ferreira dos Santos; Yueqin Wang; Javier Caballero; Primitivo Caballero; Kanglai He; Juan Jurat-Fuentes; Juan Ferré. Domain Shuffling between Vip3Aa and Vip3Ca: Chimera Stability and Insecticidal Activity against European, American, African, and Asian Pests. Toxins 2020, 12, 99 .
AMA StyleJoaquín Gomis-Cebolla, Rafael Ferreira dos Santos, Yueqin Wang, Javier Caballero, Primitivo Caballero, Kanglai He, Juan Jurat-Fuentes, Juan Ferré. Domain Shuffling between Vip3Aa and Vip3Ca: Chimera Stability and Insecticidal Activity against European, American, African, and Asian Pests. Toxins. 2020; 12 (2):99.
Chicago/Turabian StyleJoaquín Gomis-Cebolla; Rafael Ferreira dos Santos; Yueqin Wang; Javier Caballero; Primitivo Caballero; Kanglai He; Juan Jurat-Fuentes; Juan Ferré. 2020. "Domain Shuffling between Vip3Aa and Vip3Ca: Chimera Stability and Insecticidal Activity against European, American, African, and Asian Pests." Toxins 12, no. 2: 99.
Bacillus thuringiensis produces insecticidal Cry toxins used in the control of multiple insect pests. Evolution of insect resistance to Bt toxins endangers the use of Cry toxins for pest control. Analysis of the Cry1Ah-binding proteins from brush border membrane vesicles (BBMV) of Ostrinia furnacalis, Asian corn borer (ACB) from the Cry1Ah-resistant (ACB-AhR) and susceptible (ACB-BtS) strains was performed by an improved pull down assay that includes coupling Cry1Ah to NHS-activated Sepharose combined with liquid chromatography–tandem mass spectrometry (LC-MS/MS). Our data show that Cry1Ah bound to alkaline phosphatase (ALP), cadherin-like (CAD), actin, aminopeptidase-N (APN), prophenoloxidase (proPO), serine proteinase inhibitor (SPI), immulectin, and V-ATPase and to other proteins that were not previously characterized as Cry-binding proteins in ACB-BtS strain. Analysis of Cry1Ah-pulled down proteins of the BBMV from ACB-AhR revealed that Cry1Ah toxin did not bind to ALP in ACB-AhR strain, suggesting that this protein may correlate with the resistant phenotype of this strain. Additionally, we analyzed the expression of representative genes coding for Cry1Ah-binding proteins such as ALP, APN, CAD, proPO, SPI, and immulectin by qRT-PCR. ACB-AhR showed increased expression levels of proPO (7.5 fold), ALP (6.2 fold) and APN (1.4 fold) in comparison to ACB-BtS strain. In contrast, the cad gene showed slight decreased expression in ACB-AhR strain (0.7 fold) compared with ACB-BtS strain. Our data suggest that differences in the susceptibility to Cry1Ah toxin in the ACB-AhR strain may be associated with reduced ALP binding sites and with an increased immune response. This study also brings evidence of a possible binding interaction of Cry1Ah toxin to immune related proteins like proPO.
Muhammad Zeeshan Shabbir; Tiantao Zhang; Sivaprasath Prabu; Yueqin Wang; Zhenying Wang; Alejandra Bravo; Mario Soberón; Kanglai He. Identification of Cry1Ah-binding proteins through pull down and gene expression analysis in Cry1Ah-resistant and susceptible strains of Ostrinia furnacalis. Pesticide Biochemistry and Physiology 2019, 163, 200 -208.
AMA StyleMuhammad Zeeshan Shabbir, Tiantao Zhang, Sivaprasath Prabu, Yueqin Wang, Zhenying Wang, Alejandra Bravo, Mario Soberón, Kanglai He. Identification of Cry1Ah-binding proteins through pull down and gene expression analysis in Cry1Ah-resistant and susceptible strains of Ostrinia furnacalis. Pesticide Biochemistry and Physiology. 2019; 163 ():200-208.
Chicago/Turabian StyleMuhammad Zeeshan Shabbir; Tiantao Zhang; Sivaprasath Prabu; Yueqin Wang; Zhenying Wang; Alejandra Bravo; Mario Soberón; Kanglai He. 2019. "Identification of Cry1Ah-binding proteins through pull down and gene expression analysis in Cry1Ah-resistant and susceptible strains of Ostrinia furnacalis." Pesticide Biochemistry and Physiology 163, no. : 200-208.
Insects recognize odorous compounds using sensory neurons organized in olfactory sensilla. The process odor detection in insects requires an ensemble of proteins, including odorant binding proteins, olfactory receptors, and odor degrading enzymes; each of them are encoded by multigene families. Most functional proteins seem to be broadly tuned, responding to multiple chemical compounds with different, but mostly quite similar structures. Based on the hypothesis that insects recognize host volatiles by means of general odorant binding proteins (GOBPs), the current study aimed to characterize GOBPs of the yellow peach moth, Conogethes punctiferalis (Guenée). In oviposition preference tests, it was found that the yellow peach moth preferred volatiles from Prunus persica (peach) in finding their host plant. Exposure of the moth to volatiles from peaches affected the expression level of GOBP genes. Binding affinity of GOBPs from yellow peach moth was assessed for 16 host plant volatiles and 2 sex pheromones. The fluorescence ligand-binding assays revealed highest affinities for hexadecanal, farnesol, and limonene with KD values of 0.55 ± 0.08, 0.35 ± 0.04, and 1.54 ± 0.39, respectively. The binding sites of GOBPs from yellow peach moth were predicted using homology modeling and characterized using molecular docking approaches. The results indicated the best binding affinity of both GOBP1 and GOBP2 for farnesol, with scores of −7.4 and −8.5 kcal/mol. Thus, GOBPs may play an important role in the process of finding host plants.
Dapeng Jing; Tiantao Zhang; Shuxiong Bai; Sivaprasath Prabu; Kanglai He; Youssef Dewer; Zhenying Wang; Jing; Bai; He; Wang. GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, Conogethes punctiferalis (Lepidoptera: Crambidae). Insects 2019, 10, 302 .
AMA StyleDapeng Jing, Tiantao Zhang, Shuxiong Bai, Sivaprasath Prabu, Kanglai He, Youssef Dewer, Zhenying Wang, Jing, Bai, He, Wang. GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, Conogethes punctiferalis (Lepidoptera: Crambidae). Insects. 2019; 10 (9):302.
Chicago/Turabian StyleDapeng Jing; Tiantao Zhang; Shuxiong Bai; Sivaprasath Prabu; Kanglai He; Youssef Dewer; Zhenying Wang; Jing; Bai; He; Wang. 2019. "GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, Conogethes punctiferalis (Lepidoptera: Crambidae)." Insects 10, no. 9: 302.
Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) have revolutionized pest control, but the benefits of this approach have been reduced by the evolution of resistance in pests. The widely adopted 'pyramid strategy' for delaying resistance entails transgenic crops producing two or more distinct toxins that kill the same pest. The limited experimental evidence supporting this strategy comes primarily from a model system under ideal conditions. Here we tested the pyramid strategy under nearly worst-case conditions, including some cross-resistance between the toxins in the pyramid. In a laboratory selection experiment with an artificial diet, we used Bt toxins Cry1Ab, Cry1F, and Cry1Ie singly or in pairs against Ostrinia furnacalis, one of the most destructive pests of corn in Asia. Under the conditions evaluated, pairs of toxins did not consistently delay the evolution of resistance relative to single toxins.
Yueqin Wang; Yudong Quan; Jing Yang; Changlong Shu; Zhenying Wang; Jie Zhang; Angharad M. R. Gatehouse; Bruce E. Tabashnik; Kanglai He. Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs. Toxins 2019, 11, 461 .
AMA StyleYueqin Wang, Yudong Quan, Jing Yang, Changlong Shu, Zhenying Wang, Jie Zhang, Angharad M. R. Gatehouse, Bruce E. Tabashnik, Kanglai He. Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs. Toxins. 2019; 11 (8):461.
Chicago/Turabian StyleYueqin Wang; Yudong Quan; Jing Yang; Changlong Shu; Zhenying Wang; Jie Zhang; Angharad M. R. Gatehouse; Bruce E. Tabashnik; Kanglai He. 2019. "Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs." Toxins 11, no. 8: 461.
Climate change is a major environmental concern and is directly related to the increasing concentrations of greenhouse gases. The increase in concentrations of atmospheric carbon dioxide (CO2), not only affects plant growth and development, but also affects the emission of plant organic volatile compounds (VOCs). Changes in the plant odor profile may affect the plant-insect interactions, especially the behavior of herbivorous insects. In this study, we compared the foraging behavior of corn leaf aphid (Rhopalosiphum maidis) on barley (Hordeum vulgare L.) seedlings grown under contrasted CO2 concentrations. During the dual choice bioassays, the winged and wingless aphids were more attracted by the VOCs of barley seedlings cultivated under ambient CO2 concentrations (aCO2; 450 ppm) than barley seedlings cultivated under elevated CO2 concentrations (eCO2; 800 ppm), nymphs were not attracted by the VOCs of eCO2 barley seedlings. Then, volatile compositions from 14-d-old aCO2 and eCO2 barley seedlings were investigated by GC-MS. While 16 VOCs were identified from aCO2 barley seedlings, only 9 VOCs were found from eCO2 barley seedlings. At last, we discussed the potential role of these chemicals observed during choice bioassays. Our findings lay foundation for functional response of corn leaf aphid under climate change through host plant modifications.
Yu Chen; Clément Martin; Junior Corneille Fingu Mabola; François Verheggen; Zhenying Wang; Kanglai He; Frederic Francis. Effects of Host Plants Reared under Elevated CO2 Concentrations on the Foraging Behavior of Different Stages of Corn Leaf Aphids Rhopalosiphum maidis. Insects 2019, 10, 182 .
AMA StyleYu Chen, Clément Martin, Junior Corneille Fingu Mabola, François Verheggen, Zhenying Wang, Kanglai He, Frederic Francis. Effects of Host Plants Reared under Elevated CO2 Concentrations on the Foraging Behavior of Different Stages of Corn Leaf Aphids Rhopalosiphum maidis. Insects. 2019; 10 (6):182.
Chicago/Turabian StyleYu Chen; Clément Martin; Junior Corneille Fingu Mabola; François Verheggen; Zhenying Wang; Kanglai He; Frederic Francis. 2019. "Effects of Host Plants Reared under Elevated CO2 Concentrations on the Foraging Behavior of Different Stages of Corn Leaf Aphids Rhopalosiphum maidis." Insects 10, no. 6: 182.
Polyphenism, a common phenomenon in nature, is an important form of adaptation in a diverse environment. Corn leaf aphid (CLA), Rhopalosiphum maidis, (Hemiptera: Aphididae), exhibit wing polyphenism in response to poor habitat quality. In this study, we focused on the effects of crowding and thermal cues on morph determination of CLA. Five developmental stages of aphids (1st to 4th nymphs and maternal adults) with increased population densities, were tested under two kinds of temperature patterns, i.e., A) a constant temperature of 22 °C with 2 h exposure to high temperature in the range of 35 to 39 °C during mid-photophase and B) different constant temperatures in the range of 22–30 °C with 2 h exposure to high temperature of 39 °C during mid-photophase. Crowding was found to directly impact winged induction. The 1st and 2nd nymphs were more sensitive for alate morphs induction under high density. In addition, temperature played a significant role in wing production, with the temperature setting of 26/39 °C in pattern B inducing higher alate morphs and survival than other temperature settings. Therefore, we hypothesize that warmer climate with brief high temperature is more favourable for survival and alate morphs production, but cool weather and transient extreme high temperature (>39 °C) is detrimental for CLA. Our results provide a new perspective on understanding the interactions between changes in extreme high temperatures and insect densities that differentially affect wing polymorphism for further demographic and distribution rates of species across temporal and spatial scales.
Yu Chen; François Verheggen; Dandan Sun; Zhenying Wang; Frederic Francis; Kanglai He. Differential wing polyphenism adaptation across life stages under extreme high temperatures in corn leaf aphid. Scientific Reports 2019, 9, 1 -8.
AMA StyleYu Chen, François Verheggen, Dandan Sun, Zhenying Wang, Frederic Francis, Kanglai He. Differential wing polyphenism adaptation across life stages under extreme high temperatures in corn leaf aphid. Scientific Reports. 2019; 9 (1):1-8.
Chicago/Turabian StyleYu Chen; François Verheggen; Dandan Sun; Zhenying Wang; Frederic Francis; Kanglai He. 2019. "Differential wing polyphenism adaptation across life stages under extreme high temperatures in corn leaf aphid." Scientific Reports 9, no. 1: 1-8.
Background: Asian corn borer (ACB), Ostrinia furnacalis can develop resistance to transgenic Bacillus thuringiensis (Bt) maize expressing Cry1Ah-toxin. However, the mechanisms that regulate the resistance of ACB to Cry1Ah-toxin are unknown. Objective: In order to understand the molecular basis of the Cry1Ah-toxin resistance in ACB, “omics” analyses were performed to examine the difference between Cry1Ah-resistant (ACB-AhR) and susceptible (ACB-BtS) strains of ACB at both transcriptional and translational levels. Results: A total of 7,007 differentially expressed genes (DEGs) and 182 differentially expressed proteins (DEPs) were identified between ACB-AhR and ACB-BtS and 90 genes had simultaneous transcription and translation profiles. Down-regulated genes associated with Cry1Ah resistance included aminopeptidase N, ABCC3, DIMBOA-induced cytochrome P450, alkaline phosphatase, glutathione S-transferase, cadherin-like protein, and V-ATPase. Whereas, anti-stress genes, such as heat shock protein 70 and carboxylesterase were up-regulated in ACB-AhR, displaying that a higher proportion of genes/proteins related to resistance was down-regulated compared to up-regulated. The Kyoto encyclopedia of genes and genomes (KEGG) analysis mapped 578 and 29 DEGs and DEPs, to 27 and 10 pathways, respectively (P < 0.05). Furthermore, real-time quantitative (qRT-PCR) results based on relative expression levels of randomly selected genes confirmed the “omics” response. Conclusion: Despite the previous studies, this is the first combination of a study using RNA-Seq and iTRAQ approaches on Cry1Ah-toxin binding, which led to the identification of longer length of unigenes in ACB. The DEGs and DEPs results are valuable for further clarifying Cry1Ah-mediated resistance.
Muhammad Zeeshan Shabbir; Tiantao Zhang; Zhenying Wang; Kanglai He. Transcriptome and Proteome Alternation With Resistance to Bacillus thuringiensis Cry1Ah Toxin in Ostrinia furnacalis. Frontiers in Physiology 2019, 10, 27 .
AMA StyleMuhammad Zeeshan Shabbir, Tiantao Zhang, Zhenying Wang, Kanglai He. Transcriptome and Proteome Alternation With Resistance to Bacillus thuringiensis Cry1Ah Toxin in Ostrinia furnacalis. Frontiers in Physiology. 2019; 10 ():27.
Chicago/Turabian StyleMuhammad Zeeshan Shabbir; Tiantao Zhang; Zhenying Wang; Kanglai He. 2019. "Transcriptome and Proteome Alternation With Resistance to Bacillus thuringiensis Cry1Ah Toxin in Ostrinia furnacalis." Frontiers in Physiology 10, no. : 27.
GO can cause “physical damage” to the cuticle that provides a new channel for the penetration of insecticides.
Xiuping Wang; Haicui Xie; Zhenying Wang; Kanglai He; Dapeng Jing. Graphene oxide as a multifunctional synergist of insecticides against lepidopteran insect. Environmental Science: Nano 2018, 6, 75 -84.
AMA StyleXiuping Wang, Haicui Xie, Zhenying Wang, Kanglai He, Dapeng Jing. Graphene oxide as a multifunctional synergist of insecticides against lepidopteran insect. Environmental Science: Nano. 2018; 6 (1):75-84.
Chicago/Turabian StyleXiuping Wang; Haicui Xie; Zhenying Wang; Kanglai He; Dapeng Jing. 2018. "Graphene oxide as a multifunctional synergist of insecticides against lepidopteran insect." Environmental Science: Nano 6, no. 1: 75-84.
The oriental armyworm (OAW), Mythimna separata (Walker), is a destructive pest of agricultural crops in Asia and Australia. Commercialized Bt crops have performed very well against their target pests; however, very few studies have been done on the susceptibility of OAW to Bt toxins in either sprays or expressed in Bt crops. In this work, we evaluated the toxicities of Cry1Ab, Cry1Ac, Cry1Ah, Cry1Fa, Cry2Aa, Cry2Ab, Cry1Ie, Vip3Aa19, Vip3Aa16, and Vip3Ca against OAW neonate larvae, as well as the interaction between Cry and Vip toxins. The results from bioassays revealed that LC50 (lethal concentration for 50% mortality) values ranged from 1.6 to 78.6 μg/g (toxin/diet) for those toxins. Among them, Vip3 proteins, along with Cry1A proteins and Cry2Aa, were the ones with the highest potency, with LC50 values ranging from 1.6 to 7.4 μg/g. Synergism between Cry and Vip toxins was observed, being high in the combination of Vip3Aa16 with Cry1 toxins, with synergetic factors ranging from 2.2 to 9.2. The Vip3Ca toxin did not show any synergistic effect with any of the toxins tested. These results can help in designing new combinations of pyramiding genes in Bt crops, as well as in recombinant bacteria, for the control of OAW as well as for resistance management programs.
Jing Yang; Yudong Quan; Prabu Sivaprasath; Muhammad Zeeshan Shabbir; Zhenying Wang; Juan Ferré; Kanglai He. Insecticidal Activity and Synergistic Combinations of Ten Different Bt Toxins against Mythimna separata (Walker). Toxins 2018, 10, 454 .
AMA StyleJing Yang, Yudong Quan, Prabu Sivaprasath, Muhammad Zeeshan Shabbir, Zhenying Wang, Juan Ferré, Kanglai He. Insecticidal Activity and Synergistic Combinations of Ten Different Bt Toxins against Mythimna separata (Walker). Toxins. 2018; 10 (11):454.
Chicago/Turabian StyleJing Yang; Yudong Quan; Prabu Sivaprasath; Muhammad Zeeshan Shabbir; Zhenying Wang; Juan Ferré; Kanglai He. 2018. "Insecticidal Activity and Synergistic Combinations of Ten Different Bt Toxins against Mythimna separata (Walker)." Toxins 10, no. 11: 454.