Urbanisation and related insufficiency of food sources is due to the high urban population, insufficient urban food sources, and inability of some urban communities to afford food due to rising costs. Food supply can also be jeopardised by natural and man-made disasters, such as warfare, pandemics, or any other calamities which result in the destruction of crop fields and disruption of food distribution. The COVID-19 pandemic exposed the impact of such calamities on the fresh food supply chain in Malaysia, especially when the Movement Control Order (MCO) policy was first implemented. The resulting panic buying caused some food shortage, while more importantly, the fresh food supply chain was severely disrupted, especially in urban areas, in the early stages of implementation. In this regard, urban farming, while a simple concept, can have a significant impact in terms of securing food sources for urban households. It has been used in several countries such as Canada, The Netherlands, and Singapore to ensure a continuous food supply. This paper thus attempted to review how the pandemic has affected Malaysian participation in urban farming and, in relation to that, the acceptance of urban farming in Malaysia and the initiatives and approaches of local governmental and non-governmental organisations in encouraging the urban community to participate in urban farming through peer-reviewed journal articles and other articles related to urban agriculture using the ROSES protocol. About 93 articles were selected after screening to ensure that the articles were related to the study. During the COVID-19 pandemic, the surge in Malaysians’ awareness of the importance of urban farming has offered great opportunities for the government to encourage more Malaysian urban communities to participate in urban farming activities. Limitations such as relevant knowledge, area, and space, however, are impediments to urban communities’ participation in these activities. Government initiatives, such as the Urban Community Garden Policy (Dasar Kebun Komuniti Bandar (DKKB)), are still inadequate as some issues are still not addressed. Permanent Food Production Parks (TKPM) and technology-driven practices are seen as possible solutions to the primary problem of land and space. Additionally, relevant stakeholders play a crucial role in disseminating relevant and appropriate knowledge and methodology applicable for urban farming. Partnerships between government agencies, the education sector, and the private sector are necessary to develop modern urban agricultural technologies as well as knowledge, knowhow, and supports to build and sustain urban community participation in urban farming activities.

In Malaysia, the main constraints of rice yield and productivity are infertile soils and poor management practices because these soils are characterized by low pH, low nutrient availability, low organic matter, and high exchangeable Al and Fe ions, due to high rainfall and hot temperatures. Thus, an incubation study was conducted to determine the optimum amount of calcium silicate (HmbG brand) to improve the soil pH, electrical conductivity (EC), exchangeable Al, available P, and cation exchange capacity (CEC) of a paddy soil in Sabah, Malaysia. The Kelawat series (Typic Dystrudept) soil was incubated with calcium silicate at the application rates of 0 (T1), 1 (T2), 2 (T3), and 3 t ha⁻¹ (T4) using a Completely Randomized Design (CRD) in triplicates for 30, 60, 90, and 120 days. The calcium silicate used significantly improved soil pH because of the release of SiO₄⁴⁻ and Ca²⁺ ions, which neutralized and immobilized H⁺ ions. Furthermore, the neutralizing effects of the amendment impeded Al hydrolysis by up to 57.4% and this resulted in an increase in the available P in the soil by 31.26% to 50.64%. The increased availability of P in the soil was also due to the high affinity of SiO₄⁴⁻ to desorb P from soil minerals and it is believed that SiO₄⁴⁻ can temporarily adsorb exchangeable base cations such as K⁺, Ca²⁺, Mg²⁺, and Na⁺. Moreover, applying calcium silicate at 3 t ha⁻¹ improved soil CEC by up to 54.84% compared to that of untreated soils (T1) because of increased pH and the number of negatively charged sites. The most suitable application rate of the calcium silicate was found to be 3 t ha⁻¹ (T4). These findings suggest that calcium silicate can improve soil productivity and agronomic efficiency in rice farming. Greenhouse and field trials are necessary to ascertain the effects of the recommended treatments of this incubation study on soil productivity, rice growth, and yield.

Sandy-textured soil infertility poses a problem for sustainable crop cultivation. This problem is usually dealt with by the addition of chemical fertilizers, which are expensive and can cause serious environmental issues if not managed well. A better alternative to improve and manage sandy soil infertility is the use of organic amendments produced from agro-wastes. This two (2)-year field study was conducted in a sandy-textured soil grown with black pepper to evaluate mainly the effects of chemical fertilizer, fermented plant and fruit juices, compost, and biochar on soil properties and morpho-physiological characteristics and yield of black pepper. Generally, the results indicated that the combination of fermented fruit juice, compost, and biochar was comparable to and in some cases better than chemical fertilizer in terms of soil physical, chemical, and biological properties as well as crop performance. These findings were even more pronounced in the second year of measurements. There were strong positive relationships between soil N and pepper leaf chlorophyll content and between soil microbes and soil respiration. The findings showed that these soil amendments produced from agro-wastes are a good alternative to the use of chemical fertilizers on sandy soils to improve soil fertility and productivity and the yield of black pepper sustainably.

The rise in chemical fertilizer use in Malaysia raises concerns about soil degradation and potential long-term yield reductions, highlighting the importance of using organic matter for soil restoration. Azolla has been extensively studied as an alternative soil amendment due to its high nitrogen and nutrient content, as well as its rapid growth. However, the effects of fresh and composted Azolla amendments on soil chemical properties are not yet fully understood. A soil incubation study was thus conducted to determine the effects of fresh and composted Azolla on soil chemical properties over a 3-month incubation period. The soil treatments consisted of non-amended soil (control); fresh Azolla at 3, 6, and 9% w/w; and composted Azolla at 1, 2, and 3% w/w, with soil water holding capacity maintained at 55% throughout the incubation period. The collected soil samples were analyzed for soil pH and electrical conductivity (EC), total carbon (C) and nitrogen (N), available phosphorus, exchangeable bases—potassium (K), calcium, and magnesium, using inductively coupled plasma optical emission spectrometry, and cation exchange capacity (CEC). All data were subjected to variance analysis for statistical analysis. The study revealed significant effects of interaction between soil treatments and incubation periods for all soil parameters. At the end of the incubation period, the soil treated with 3% composted Azolla exhibited higher soil EC, total C and N, exchangeable K, and CEC compared to other soil treatments. The 3% fresh Azolla treatments were also observed to improve the soil’s exchangeable calcium by the end of the incubation period. In conclusion, 3% composted Azolla is best to help restore soil nutrient levels for crop uptake.

2S albumin seed storage proteins from Theobroma cacao (Tc-2S) are known for their roles in plant defense mechanisms due to their antimicrobial properties. However, it remains unclear whether both the light and heavy chains of Tc-2S are required for this activity. This study develops an expression system for the single-chain precursor of Tc-2S and evaluate its antimicrobial activity. Specifically, the heavy-chain subunit (Tc-9M), corresponding to residues 78 to 150 of the Tc-2S precursor, was cloned and expressed in a heterologous system. The resulting Tc-9M protein, expressed as a fully soluble protein, was purified via column chromatography, yielding 24 mg of pure protein from 300 mL of the expression culture. Antibacterial and antifungal activity was assessed using the Kirby-Bauer disc diffusion method, revealing that Tc-9M remarkably inhibited the growth of several bacterial strains, including Salmonella sp., Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus. Additionally, Tc-9M inhibited the growth of the yeasts Saccharomyces cerevisiae and Pichia pastoris but showed no activity against the fungus Trichoderma asperellum. These findings ndicate that the single-chain Tc-2S may be sufficient for antimicrobial defense in plants. Because the characterization of the Tc-9M protein has not been previously reported, this work provides a basis for further exploration of the biological significance of the 2S albumin subunit in plant defense. Keywords: 2S albumin protein, antibacterial, antifungal, heavy-chain peptide, Theobroma cacao