Lead (Pb) is one of the toxins responsible for the deterioration of ecological health in aquatic environments. The present study investigated the effects of Pb(NO₃)₂ toxicity on growth, blood cell morphology, and the histopathology of gills, liver, and intestine of juvenile Nile tilapia, Oreochromis niloticus. A 30-day long aquarium trial was conducted by assigning three treatment groups T₁ 5.20 mg L⁻¹, T₂ 10.40 mg L⁻¹, and T₃ 20.80 mg L⁻¹, and a control 0 mg L⁻¹ following the 96 h LC₅₀ of 51.96 mg L⁻¹ from acute toxicity test. Overall growth performance significantly declined in all the Pb(NO₃)₂ treated groups and the highest mortality was recorded in T₃. Behavioural abnormalities were intense in all the treatment groups compared to the control. Hepatosomatic index (HSI) values were reported as higher in treatment groups. Reduced nucleus diameter and nuclei size in erythrocytes were reported for T₂ and T₃ groups. Dose-dependent histological alterations were visible in the gills, liver, and intestine of all the Pb(NO₃)₂ treated groups. The width of the intestinal villi was highly extended in T₃ showing signs of severe histological alterations. In conclusion, Pb toxicity causes a negative effect on growth performance, erythrocyte morphology, and affected the vital organs histomorphology of juvenile O. niloticus.

Plastic pollution is one of the pressing issues in freshwater ecosystems that may further contribute to coastal pollution. The present study aimed to address the state of macroplastics pollution in the Surma River system, Bangladesh. Six sampling sites were allocated in the river starting from upstream to downstream, water parameters and fin fish assemblage were recorded, and plastic debris was collected from each site. Afterward, macroplastics were categorized and weighed to measure their abundance. Previous data on rainfall, water discharge, and depth were aggregated to study the trend of river depth changes. A survey was conducted to identify the possible sources of plastic pollution in the river and awareness of the pollution. The results showed that Kazir Bazar (Site 4) and Beter Bazar (Site 5), comparatively contained poor water quality, diverse macroplastics categories, and higher macroplastics abundance. The water pollution index (WPI) also ranked the above sites as extremely polluted. Similarly, biodiversity indices revealed lower diversity at Site 4 and Site 5. The river depth analysis revealed that there was no remarkable tendency to change the depth. To conclude, the Surma River system is being polluted due to inadvertent plastic dumping. Contemporary awareness is highly required, and proper policies should be implemented to minimize the detrimental effects of macroplastics.

Pb is one of the most extensively used harmful heavy metals in Bangladesh, and its occurrence in waters affects aquatic organisms significantly. The tropical pearl mussel, Lamellidens marginalis, was exposed to different concentrations (T₁ 21.93 mgL⁻¹, T₂ 43.86 mgL⁻¹, and T₃ 87.72 mgL⁻¹) of Pb(NO₃)₂ and was evaluated against a control C 0 mgL⁻¹ of Pb(NO₃)₂, followed by a 96 h acute toxicity test. The LC₅₀ value was recorded as 219.32 mgL⁻¹. The physicochemical parameters were documented regularly for each treatment unit. The values of % SGR, shell weight, soft tissue wet weight, and weight gain remained statistically higher for the control group in comparison with the treatment. No mortality was noted for control units, while a gradually decreased survival rate was recorded for the different treatment groups. Fulton’s condition factor was recorded as highest in the control and lowest in the T₃ unit, while the condition indices did not vary between the control and treatment groups. The hemocyte was accounted as maximum in the control and T₁, while minimum in T₂ and T₃. The serum lysosomal parameters also followed a similar pattern, and a significantly low level of lysosomal membrane stability, and serum lysosome activity was noted for T₃ and T₂ units in comparison to the control group. The histology of the gill, kidney, and muscle was well structured in the control group, while distinct pathologies were observed in the gill, kidney, and muscle tissue of different treatment groups. The quantitative comparison revealed that the intensity of pathological alteration increased as the dosage of Pb increased. The current study, therefore, indicated that intrusion of Pb(NO₃)₂ in the living medium significantly alters growth performance and hemocyte counts, and chronic toxicity induces histomorphological abnormalities in vital organs.

RNA interference (RNAi)-based biotechnology has been previously implemented in decapod crustaceans. Unlike traditional RNAi methodologies that investigate single gene silencing, we employed a multigene silencing approach in decapods based on chimeric double-stranded RNA (dsRNA) molecules coined ‘gene blocks’. Two dsRNA constructs, each targeting three genes of the crustacean hyperglycaemic hormone (CHH) superfamily of neuropeptides, were produced: Type II construct targeting Cq-Molt-inhibiting hormone 1 (MIH1), Cq-MIH-like 1 (MIHL1), and Cq-MIHL2 isoforms and Type I construct targeting Cq-ion transport peptide (Cq-ITP; a putative hybrid of CHH and MIH) and Cq-CHH and Cq-CHH-like (CHHL) isoforms. Both constructs were injected into juvenile redclaw crayfish, Cherax quadricarinatus, to determine the effects of multigene knockdown on molting and developmental processes. A 20-Hydroxyecdysone (20E) enzyme-linked immunosorbent assay (ELISA) and glucose assay were used to determine the effects of RNAi on molting and hemolymph glycemic activities, respectively. Multigene silencing reduced the intermolt interval by 23%. Statistically significant elevated 20E was recorded in treated intermolt individuals, consistent with the reduced intermolt interval as well as unique and abnormal phenotypes related to the molting process, which indicates a shift in 20E-induced cascade. There was no effect of RNAi treatment on hemolymph glucose level or molt increment. Through multigene silencing and subsequent annotation of gene networks, gene blocks may provide a tailored approach to investigate complex polygenic traits with RNAi in a more efficient and scalable manner.