Support and management of second-intention wound healing involves frequent dressing changes having different properties. Dressings can range from simple ones, such as nonadherent dressings, to more complex options, such as foam, hydrocolloid, alginate or negative pressure dressings. Seven cats were enrolled in the study with a total of nine wounds of various sizes with different etiology sizes and localizations. Three methods of local ozone administration were used to cover more of the ozone properties in the treatment of wounds: bagging, perilesional subcutaneous infiltrations and lavages with ozonated saline. Evaluation of the healing process was performed by clinical observation and wound area measurements every seven days until the complete recovery of the patients. The results of this study should encourage clinicians to consider medical ozone as a new therapeutic approach with regenerative properties in the second-intention healing of cats presenting cutaneous wounds.

The search for broad-spectrum antimicrobial products that do not generate resistance upon multiple applications has led to increased scientific and clinical interest in ozonated oils. The aim of this preliminary study was to evaluate the physico-chemical structure and antimicrobial properties of 1–12 h ozonated extra virgin olive oil produced in a veterinary clinic with a medical-grade generator. Prolonging the ozonation time causes a decrease in the iodine index, followed by significant increases in viscosity, acidity index, and peroxide values (p < 0.001). Other similar studies using industrial generators obtained satisfactory clinical results at peroxide values between 335 and 3590 mEq O₂/Kg. Contrary to these established minimum thresholds, we found that ozonated olive oil with a peroxide index of 184 and 224 mEq O₂/Kg exhibits fungicidal and bactericidal effects, demonstrating significant differences (p < 0.05) between tested and control samples for strains such as Staphylococcus aureus, E. faecalis, and E. coli. The 12 h ozonated oil showed itself to be efficient in the treatment of a 3-year-old cat presenting a chronic infected wound. The results encourage more detailed investigations of the antimicrobial effect of ozonated oils obtained with medical-grade generators and their evaluation on bacterial strains isolated from different individuals, followed by clinical evaluations and standardization.

This case report describes a new therapeutic approach for a domestic shorthaired female cat, who has an extensive posttraumatic wound in the right hind limb. After patient stabilization, general anesthesia was started and the wound was cleaned and debrided of devitalized tissues, followed by the application of ozone therapy and bandage. Eight sessions of ozone therapy were performed for 17 days until the application of the skin graft. Three more sessions of ozone therapy were performed every 3 days postoperatively. The bagging method and the perilesional infiltration method were used. The ozone therapy ensured an accelerated recovery of the patient without any complications. According to our knowledge, this is the first case report with the use of ozone therapy to support a free skin graft in a cat. The new therapeutic approach could be used to accelerate healing of the wounds with a significant lack of substance, by supporting pre- and post-operative skin grafts.

The therapeutic management of extensive skin wounds in cats can be time-consuming and require multiple therapeutic interventions, which can have significant financial implications for pet owners. Reconstructive surgery is often necessary to close skin defects with tissue loss to provide a quicker patient recovery. Conventional therapies like systemic antibiotics, anti-inflammatories, and local dressings are not always successful due to antibiotic resistance or a poor response, such as no or delayed healing. For more than a century, ozone has been utilized as an excellent disinfectant, but caution should be taken due to its oxidizing properties. Only in the past decade have numerous studies established therapeutic dose ranges for a wider medical use of ozone. The objective of this study was to clinically evaluate ozone therapy as a complementary treatment supporting and completing plastic and reconstructive surgery in 4 cats with extensive skin defects. The results obtained, following the local application of ozone therapy before and after skin reconstruction in our patients, encourage the use of ozone as a complementary therapy in the management of extensive skin wounds treated surgically by different reconstructive techniques.

Limb wounds in horses represent a significant therapeutic challenge due to poor vascularization, reduced skin elasticity, and high risk of complications such as exuberant granulation tissue. Conventional treatments sometimes fail to provide satisfactory healing outcomes, leading to prolonged recovery and increased costs. This study aimed to evaluate the efficacy of topical ozone therapy using the bagging method in promoting the epithelialization and contraction of chronic distal limb wounds in horses refractory to conventional management. Eight horses, aged 3–21 years, with chronic wounds averaging 48.79 ± 21.20 cm², were treated exclusively with ozone (50 μg/mL) administered by bagging for 30 min every 48 h until complete healing. All cases achieved full wound closure within 27–91 days without systemic medication or major complications. Macroscopic evaluation showed favorable healing, with the restoration of skin pigmentation and hair growth in most cases, while only minimal fibrous scarring was observed in a few patients. Statistical analysis revealed significant improvements in epithelialization, particularly during the last four weeks of treatment. These findings suggest that ozone bagging therapy is a simple, cost-effective, and well-tolerated method that may enhance the healing of chronic distal limb wounds in horses. Further controlled trials are needed to standardize treatment protocols and compare ozone with conventional therapies.