This study seeks to understand the effects of new hydrogels based on Oregano^® essential oil, Frankincense^® essential oil, and the Thieves^® blend, which includes clove, lemon, cinnamon bark, eucalyptus radiata, rosemary extract, and Frankincense^® essential oil through in vitro cytotoxicity and antimicrobial analysis. Mesenchymal stem cells (D1MSCs) generated from the dental papilla of human wisdom teeth germs were used to test the hydrogels’ cytotoxicity (D1MSCs). The chemical makeup of the tested essential oils (EO) was determined using GC-MS analysis, and their presence in the novel hydrogels was determined using UV-VIS analysis. The primary constituents of the essential oils identified as being present were eugenol, pinene, limonene, carvacrol, and cymene. The primary constituents from essential oils identified by the absorption spectra included eugenol, pinene, limonene, carvacrol, and cymene. The presence of essential oils in the hydrogel composition was also clearly discernible. All of the studied compounds had a reduced cytotoxic effect on cell cultures, proving the lack of in vitro toxicity of the gels. This study demonstrates that hydrogels enriched with pure natural extracts of essential oils have a tangible in vitro antimicrobial potential, especially for Staphylococcus aureus and Bacillus cereus.

Veterinary minimally invasive surgery (MIS) has experienced notable growth in recent years, yet the availability of specialized training tools remains limited and not readily accessible to practitioners worldwide. While borrowing simulators from human medicine practices suffices for acquiring fundamental laparoscopic skills, it proves inadequate when addressing procedure-specific nuances. Veterinary professionals are now taking steps to create simulators tailored to their patients, although the validation process can be time-consuming. Consequently, the availability of advanced laparoscopic simulators for veterinary training remains scarce. The present study aims to highlight custom-made simulators. A comprehensive search across five databases was conducted to uncover the simulators documented from 2010 to 2022. A total of five simulators emerged from this search, with four grounded in a canine model and only one in an equine model. These models underwent validation and were found to be effective in training surgeons for their designated tasks. The findings underscore a limited array of simulators, predominantly catering to two species (horses and dogs). Considering these findings, it is evident that further research is imperative to create laparoscopic simulators capable of facilitating advanced veterinary training. This would enable the continued evolution of surgical techniques across diverse species, including ruminants, small mammals, and non-mammalian animals.

Limb wounds in horses represent a significant therapeutic challenge due to poor vascularization, reduced skin elasticity, and high risk of complications such as exuberant granulation tissue. Conventional treatments sometimes fail to provide satisfactory healing outcomes, leading to prolonged recovery and increased costs. This study aimed to evaluate the efficacy of topical ozone therapy using the bagging method in promoting the epithelialization and contraction of chronic distal limb wounds in horses refractory to conventional management. Eight horses, aged 3–21 years, with chronic wounds averaging 48.79 ± 21.20 cm², were treated exclusively with ozone (50 μg/mL) administered by bagging for 30 min every 48 h until complete healing. All cases achieved full wound closure within 27–91 days without systemic medication or major complications. Macroscopic evaluation showed favorable healing, with the restoration of skin pigmentation and hair growth in most cases, while only minimal fibrous scarring was observed in a few patients. Statistical analysis revealed significant improvements in epithelialization, particularly during the last four weeks of treatment. These findings suggest that ozone bagging therapy is a simple, cost-effective, and well-tolerated method that may enhance the healing of chronic distal limb wounds in horses. Further controlled trials are needed to standardize treatment protocols and compare ozone with conventional therapies.

Grade IV lateral patellar luxation is an advanced orthopedic condition that may be associated with severe skeletal deformities, chronic soft-tissue contracture, and irreversible loss of normal stifle function. Although reconstructive procedures are considered the treatment of choice in most dogs, some end-stage cases may not be amenable to conventional correction. This report describes the clinical presentation, surgical management, and long-term outcome of a young dog with severe bilateral grade IV lateral patellar luxation treated with single-stage bilateral stifle arthrodesis. A 1-year-old, 42-kg intact female Romanian Shepherd Dog was presented with chronic severe hindlimb dysfunction, crouched posture, and permanent bilateral patellar luxation. Orthopedic and radiographic examination revealed severe femoral valgus, proximal tibial varus, external tibial torsion, absence of a functional trochlear groove, marked caudal thigh muscle contracture, and severely restricted stifle extension. Computed tomography was considered but not performed because of financial limitations. Based on the severity of the deformities and the limited preoperative range of motion, conventional reconstructive procedures were considered unlikely to restore functional joint motion. Bilateral stifle arthrodesis was therefore performed during a single anesthetic event using tibial tuberosity osteotomy, temporary Kirschner-wire fixation, and bilateral 3.5-mm locking compression plates. Due to severe chronic soft-tissue contracture, final arthrodesis angles of 95° and 99° were achieved. Progressive clinical improvement and radiographic bone union were observed throughout follow-up. At 12 months, complete bilateral arthrodesis and implant stability were confirmed radiographically. At 24 months, the dog remained independently ambulatory with a markedly improved quality of life despite persistent mechanical gait abnormalities, including circumduction and intermittent bunny-hopping. This case suggests that bilateral stifle arthrodesis may represent a salvage option in carefully selected dogs with end-stage bilateral grade IV lateral patellar luxation when conventional reconstructive procedures are unlikely to restore useful limb function.

The use of an omental pedicle flap as an adjuvant treatment has gained popularity in veterinary medicine due to its versatility. This technique has been successfully applied in both intra-abdominal and extra-abdominal surgeries in human as well as veterinary medicine. In most intra-abdominal procedures, the greater omentum can be easily accessed through a midline laparotomy and directed to the area of interest. However, dogs and cats are typically positioned in lateral recumbency for extra-abdominal surgeries involving the thorax or limbs. In such cases, a lateral approach to the greater omentum could minimize the risk of infection or trauma associated with patient repositioning. This cadaver study evaluated the feasibility of accessing the greater omentum through a lateral approach laparotomy in both left and right lateral recumbency. A total of 22 cadavers (13 cats and 9 dogs) were included. Dorsal extension of the greater omentum was successfully performed in all specimens without major structural damage. Further elongation using an inverted L-shaped pedicle flap was achieved without rupture in 17 of 22 cadavers (77.3%). The results demonstrate that lateral approach laparotomy provides reliable access to the greater omentum and allows successful mobilization of the omentum for potential extra-abdominal applications, with no significant differences observed between left and right lateral approaches. This technique may represent a useful alternative in clinical situations where repositioning the patient is not feasible or carries increased surgical risk.