This study seeks to understand the effects of new hydrogels based on Oregano^® essential oil, Frankincense^® essential oil, and the Thieves^® blend, which includes clove, lemon, cinnamon bark, eucalyptus radiata, rosemary extract, and Frankincense^® essential oil through in vitro cytotoxicity and antimicrobial analysis. Mesenchymal stem cells (D1MSCs) generated from the dental papilla of human wisdom teeth germs were used to test the hydrogels’ cytotoxicity (D1MSCs). The chemical makeup of the tested essential oils (EO) was determined using GC-MS analysis, and their presence in the novel hydrogels was determined using UV-VIS analysis. The primary constituents of the essential oils identified as being present were eugenol, pinene, limonene, carvacrol, and cymene. The primary constituents from essential oils identified by the absorption spectra included eugenol, pinene, limonene, carvacrol, and cymene. The presence of essential oils in the hydrogel composition was also clearly discernible. All of the studied compounds had a reduced cytotoxic effect on cell cultures, proving the lack of in vitro toxicity of the gels. This study demonstrates that hydrogels enriched with pure natural extracts of essential oils have a tangible in vitro antimicrobial potential, especially for Staphylococcus aureus and Bacillus cereus.

Support and management of second-intention wound healing involves frequent dressing changes having different properties. Dressings can range from simple ones, such as nonadherent dressings, to more complex options, such as foam, hydrocolloid, alginate or negative pressure dressings. Seven cats were enrolled in the study with a total of nine wounds of various sizes with different etiology sizes and localizations. Three methods of local ozone administration were used to cover more of the ozone properties in the treatment of wounds: bagging, perilesional subcutaneous infiltrations and lavages with ozonated saline. Evaluation of the healing process was performed by clinical observation and wound area measurements every seven days until the complete recovery of the patients. The results of this study should encourage clinicians to consider medical ozone as a new therapeutic approach with regenerative properties in the second-intention healing of cats presenting cutaneous wounds.

The aim of this research was to validate the use of a gentamicin (GEN) and nano-hydroxiapatite (nHAP)-loaded polycaprolactone nanostructured membrane (NM) as an innovative, highly efficient, low-cost treatment for periodontitis. We conducted an in vivo study on Wistar rats, in which we induced periodontitis by placing silk ligatures around the first right and left upper molars. The subjects were divided into three groups; the first group received no periodontal treatment, the second group received open flap debridement, and the third group received open flap debridement, together with the positioning of the GEN and nHAP-loaded nanostructured membrane as a treatment. The extent of periodontal regeneration was assessed by the periodontal pocket depth, bleeding on probing, tooth mobility, dental plaque, microbiological analysis, concentration of MMP-8 in saliva, plasma levels of CRP, and histological analysis. The results showed that using open flap debridement with the NM is more efficient, and it significantly reduces the probing depth, extent of bleeding on probing, dental mobility, bacterial plaque, and pathogenic flora. The concentrations of MMP-8 and CRP decrease. The histological analysis demonstrated that NM leads to bone regeneration. Our study indicates that gentamicin and nano-hydroxyapatite embedded in the fiber of the biodegradable membranes might be a promising therapeutic option for periodontitis treatment.

This case report describes a new therapeutic approach for a domestic shorthaired female cat, who has an extensive posttraumatic wound in the right hind limb. After patient stabilization, general anesthesia was started and the wound was cleaned and debrided of devitalized tissues, followed by the application of ozone therapy and bandage. Eight sessions of ozone therapy were performed for 17 days until the application of the skin graft. Three more sessions of ozone therapy were performed every 3 days postoperatively. The bagging method and the perilesional infiltration method were used. The ozone therapy ensured an accelerated recovery of the patient without any complications. According to our knowledge, this is the first case report with the use of ozone therapy to support a free skin graft in a cat. The new therapeutic approach could be used to accelerate healing of the wounds with a significant lack of substance, by supporting pre- and post-operative skin grafts.

The search for broad-spectrum antimicrobial products that do not generate resistance upon multiple applications has led to increased scientific and clinical interest in ozonated oils. The aim of this preliminary study was to evaluate the physico-chemical structure and antimicrobial properties of 1–12 h ozonated extra virgin olive oil produced in a veterinary clinic with a medical-grade generator. Prolonging the ozonation time causes a decrease in the iodine index, followed by significant increases in viscosity, acidity index, and peroxide values (p < 0.001). Other similar studies using industrial generators obtained satisfactory clinical results at peroxide values between 335 and 3590 mEq O₂/Kg. Contrary to these established minimum thresholds, we found that ozonated olive oil with a peroxide index of 184 and 224 mEq O₂/Kg exhibits fungicidal and bactericidal effects, demonstrating significant differences (p < 0.05) between tested and control samples for strains such as Staphylococcus aureus, E. faecalis, and E. coli. The 12 h ozonated oil showed itself to be efficient in the treatment of a 3-year-old cat presenting a chronic infected wound. The results encourage more detailed investigations of the antimicrobial effect of ozonated oils obtained with medical-grade generators and their evaluation on bacterial strains isolated from different individuals, followed by clinical evaluations and standardization.

The therapeutic management of extensive skin wounds in cats can be time-consuming and require multiple therapeutic interventions, which can have significant financial implications for pet owners. Reconstructive surgery is often necessary to close skin defects with tissue loss to provide a quicker patient recovery. Conventional therapies like systemic antibiotics, anti-inflammatories, and local dressings are not always successful due to antibiotic resistance or a poor response, such as no or delayed healing. For more than a century, ozone has been utilized as an excellent disinfectant, but caution should be taken due to its oxidizing properties. Only in the past decade have numerous studies established therapeutic dose ranges for a wider medical use of ozone. The objective of this study was to clinically evaluate ozone therapy as a complementary treatment supporting and completing plastic and reconstructive surgery in 4 cats with extensive skin defects. The results obtained, following the local application of ozone therapy before and after skin reconstruction in our patients, encourage the use of ozone as a complementary therapy in the management of extensive skin wounds treated surgically by different reconstructive techniques.