Runs of homozygosity (ROH) are contiguous stretches of homozygous genotypes that are passed from parents to their offspring. ROHs are suitable for determining population history, inbreeding rates, and the genetic relationships between individuals in the populations, as well as to detect candidate genes responsible for economic traits in farm animals. In this study, we observed that the Kazakh white-headed (KWh) cattle breed (ROH n = 55,976) had a higher number of ROH compared to the Auliekol (AK) breed (ROH n = 13,137). When calculating the mean length of ROH, there were considerable differences between Kazakh white-headed (211.59 ± 92.98 Mb) and Auliekol (99.62 ± 46.48 Mb) populations. The maximum length of ROH was higher in Auliekol cattle (510.25 Mb) than in Kazakh white-headed cattle (498.91 Mb). The average inbreeding coefficient rate was equal to 0.084 ± 0.037 in Kazakh white-headed cattle and 0.039 ± 0.018 in Auliekol cattle. The high frequency of genomic regions showed that the strongest patterns were observed on chromosomes 2, 6, and 26 for KWh and 1, 5, and 14 for AK. The estimation of ROH numbers per animal showed that the number of ROH decreased with increasing ROH length in both populations. The genomic inbreeding coefficient of both cattle breeds was calculated based on the ROH, and ancient inbreeding was observed. The harbored genes within ROH islands were associated with meat growth and milk production.

Kazakh white-headed and Auliekol breeds are mostly spread through Kazakhstan and are animals with dual purpose productivity directions including meat and milk. Kazakh native cattle breeds are still largely unexplored based on the genetic markers and are of great interest in the country. In this study, we for the first time applied high-density SNP genotyping for two indigenous cattle breeds to study genetic diversity, population structure and relationships with the different foreign breeds. Using of GGP Bovine 150K SNP array the principal component analysis (PCA), populations estimates of the genetic structure (ADMIXTURE), genetic diversity and distances (Fst), and phylogenetic tree showed that the Kazakh white-headed and Auliekol breeds have certain separate genetic structure that differ from European and Russian cattle breeds The two of these Kazakh cattle breeds have their genetic background according to observed results as distinct breeds. The obtained results will be aided in the development breeding system and conservation programs of the Kazakh white-headed and Auliekol breeds of beef cattle in the Kazakhstan.

A study on the study of the qualitative assessment of the behavior of dairy cattle according to the VAS system (visual analog scale) was conducted in the LLP "Agrofirme "AKAS", located in the West Kazakhstan region of the Republic of Kazakhstan. According to research, it follows that different seasons of time affect the conditions of detention in different ways. So, for the maintenance of cows in the summer, the distance of avoidance at the feeding site according to test 1 and 2 is within 3 meters, whereas in spring and autumn it was 2 meters. The difference between the reference norm and the household ration has been determined, from which it follows that the feeding ration of the farm corresponds to the norm of 12 kg of milk yield per head per day. The vitamin and mineral premix developed by us includes vitamins, minerals, including macro-microelements. The premix also includes components in the following ratio: phosphorus (P) – 5% of monocalcium phosphate, vitamin A – 1.2% of rovimix A 1000, vitamin D3 – 0.64% of rovimix D3, vitamin E – 16% of rovimix E-50, manganese (Mn) – 22.2%, copper (Cu) – 9%, zinc (Zn) – 19%, iodine (I) – 0.28%, selenium (Se) – 7.65 and cobalt (Co) – 0.14%, and feed chalk – 5% and chickpea flour – 13.89% are used as fillers as a source of calcium (Ca).. The average daily milk yield at the beginning of the experiment in both study groups was in the range of 10.6-10.8 kg. At the end of the experiment, the average daily milk yield in animals receiving premix exceeded their peers from the control group by 1.4 kg or 1.6%. According to the chemical composition of milk, the indicators of fat, protein and lactose were higher in the cows of the experimental group compared with the peers of the control group (for fat – 3.92>3.37, for protein – 3.32>3.23, for lactose - 4.16>4.01).

This article presents the results of the culling of cows of the Simmental and Holstein breeds in the Republic of Kazakhstan. The purpose of the research was to study the actual number of culled cows of dairy productivity in the farms of the Republic of Kazakhstan to determine its norm. The object of research were cows of Simmental (18,462 heads) and Holstein (17,862 heads) breeds in various regions of the republic. The research was carried out according to generally accepted zootechnical methods using statistical data processing using RStudio. The results of the studies showed that the percentage of culling increases in cows of the Simmental and Holstein breeds and amounts to 19.7 and 22.9%, respectively. The analysis of the retirement of full-aged cows of the Simmental breed showed that the main causes are hoof diseases (up to 26%), low reproductive function (up to 21%), low productivity (up to 18%). Holstein cows had an increase in disposals in all categories except for disposals for various reasons, for problems with reproduction, the disposal is up to 21%. The determination of the relationship between productivity and retirement in the Simmental breed cows showed that with age and increased lactation, the proportion of retired cows also increases, in the Holstein breed, with increased productivity, the percentage of culled animals also increases.

The high milk productivity of cows with an inadequate feeding level is the cause of many animal diseases. To control protein and energy in feeding ration it may be used as an indicator the milk urea content (Nousiainen, J.K.J. Shingfield, and P. Huhtanen, 2004). The norm of its content is in the range of 15–30 mg% (Smith, J., G. Verkerk, B. McKay, 2000). The purpose of the work was to introduce milk urea indicator in Republic of Kazakhstan by the experience of USA and Canada milk labs. Research work was carried out under project “Improving the breeding methods efficiency.” The studies were carried out using infrared analyzer CombiFoss FT +. The results of the study are shown in table 1. As you can see, milk urea content in Agrofirm Rodina LLP was 34.25 ± 0.29 mg%. Analysis of cows diet in this farm showed, there it was protein excess by 7.9% in comparison with the norms. In the second farm, Esil-Agro LLP, it was a different case. Milk urea content was 11.7 mg%. Low level of urea in this case was the result of energy and protein lack in the diet of dairy cows. It can be concluded that in conditions of dairy farms in the Republic of Kazakhstan, milk urea can serve as reliable indicator of protein and energy level in the diets of dairy cows, monitoring its content will ensure the rational use of expensive protein feeds, preserving animal health and thereby increase the efficiency of milk production.