Background: Complex regional pain syndrome type-I (CRPS-I) causes excruciating pain that affect patients’ life quality. However, the mechanisms underlying CRPS-I are incompletely understood, which hampers the development of target specific therapeutics. Methods: The mouse chronic post-ischemic pain (CPIP) model was established to mimic CRPS-I. qPCR, Western blot, immunostaining, behavioral assay and pharmacological methods were used to study mechanisms underlying neuroinflammation and chronic pain in spinal cord dorsal horn (SCDH) of CPIP mice. Results: CPIP mice developed robust and long-lasting mechanical allodynia in bilateral hindpaws. The expression of inflammatory chemokine CXCL13 and its receptor CXCR5 was significantly upregulated in ipsilateral SCDH of CPIP mice. Immunostaining revealed CXCL13 and CXCR5 was predominantly expressed in spinal neurons. Neutralization of spinal CXCL13 or genetic deletion of Cxcr5 (Cxcr5^−/−) significantly reduced mechanical allodynia, as well as spinal glial cell overactivation and c-Fos activation in SCDH of CPIP mice. Mechanical pain causes affective disorder in CPIP mice, which was attenuated in Cxcr5^−/− mice. Phosphorylated STAT3 co-expressed with CXCL13 in SCDH neurons and contributed to CXCL13 upregulation and mechanical allodynia in CPIP mice. CXCR5 coupled with NF-κB signaling in SCDH neurons to trigger pro-inflammatory cytokine gene Il6 upregulation, contributing to mechanical allodynia. Intrathecal CXCL13 injection produced mechanical allodynia via CXCR5-dependent NF-κB activation. Specific overexpression of CXCL13 in SCDH neurons is sufficient to induce persistent mechanical allodynia in naïve mice. Conclusions: These results demonstrated a previously unidentified role of CXCL13/CXCR5 signaling in mediating spinal neuroinflammation and mechanical pain in an animal model of CRPS-I. Our work suggests that targeting CXCL13/CXCR5 pathway may lead to novel therapeutic approaches for CRPS-I.

The utilization of waste rubber tires is of great value for environment protection and resource recovery, which can also improve the properties of matrix asphalt. The adhesion characteristics were evaluated for crumb rubber modified asphalt and limestone aggregate using the surface free energy (SFE) approach. Four types of matrix asphalt and four rubber contents were used to prepare the crumb rubber modified asphalt. The contact angle of matrix and crumb rubber modified asphalt was obtained, and the SFE indicators (dispersion, polar component, and compatibility rate—CR) were calculated. Moreover, the water stability tests were conducted using one matrix and rubber modified asphalt in order to investigate the relationship between SFE and water stability indicators. Results showed that the total SFE, dispersion component, adhesion work, and CR increased with the addition of crumb rubber, while the polar component and spalling work decreased. The types of asphalt had different influences on SFE indicators. The results from analysis of variation (ANOVA) indicated asphalt type and rubber content had significant influence on the adhesion work, spalling work and CR, and the influence of asphalt type was greater than that of rubber content. Additionally, the retained Marshall Stability and tensile strength ratio had better correlation with adhesion work and CR, but less with spalling work. The presented results demonstrated that the type of matrix asphalt played an important role in the adhesion characteristics for the crumb rubber modified asphalt.

To evaluate the effect of rejuvenating agent on reclaimed SBS (styrene-butadiene-styrene) modified asphalt pavement (RSMAP) material, different tests of asphalt and mixtures were conducted. Firstly, the improvement effect of rejuvenating agents on the aged modified asphalt was tested at macroscopic and microscope level. Then the properties of hot mix asphalt (HMA) with different RSMAP contents (0%, 30%, 50% and 70%) were evaluated by conducting freeze-thaw split, semi-circular bending (SCB) and dynamic modulus (DM). The results indicate that rejuvenating agent can recycle the properties of aged modified asphalt effectively. The initial phase structure of the aged modified asphalt is not changed by adding rejuvenating agents. Moreover, the SBS particles area ratio of modified asphalt blends is significantly correlated with tenacity as the proportion of rejuvenating agent increases. For mixtures, RSMAP is harmful to moisture susceptibility and low-temperature cracking resistance of recycled mixture, especially with high RSMAP content. Moreover, the two properties can be improved by adding rejuvenating agents, but the recycled mixtures with high RSMAP content are not easy to recover to HMA mixture level. In general, the rejuvenating agent has an effect on the stiffness of the recycled mixture, but this is effect not obvious. When loading frequency reaches a higher value, the DM of recycled mixtures and HMA mixture tend to be consistent including high RSMAP content.

For the concerns of investigating the viscoelastic properties of recycled asphalt mixtures incorporating high content reclaimed styrene-butadiene-styrene (SBS) modified asphalt pavement (RAP-SBS), asphalt mixture performance tester (AMPT) was applied to analyze the dynamic modulus and phase angle of recycled mixtures by the influence of RAP-SBS content, temperature, loading frequency, long-term aging (LOTA), and the incorporation of a rejuvenating agent. Master curves of recycled asphalt mixture regarding dynamic modulus and phase angle are developed, and the viscoelastic properties of recycled mixtures within a wide frequency range are characterized with the Christensen–Anderson–Marastean (CAM) model. Eventually, the one-way analysis of variance (ANOVA) was applied to investigate the role of factors on the viscoelasticity of recycled mixtures. The research indicates that (1) the elastic component of recycled mixtures elevates with the increasing of RAP-SBS content and loading frequency; as a result, the high-temperature stability of it enhances, while it is prone to cracking at low temperatures; (2) RAP-SBS content should be selected according to specific characteristics of pavement. For most cases, a content of 50% is recommended; (3) the recycled mixtures incorporating high-content RAP-SBS mixed with a rejuvenating agent has outstanding aging resistance performance; (4) RAP-SBS content is observed to have a significant influence on the viscoelasticity of recycled mixtures.

Refined separation not only controls the variability of reclaimed asphalt pavement (RAP), but also improves the mixing ratio of RAP and the quality of recycled asphalt mixtures. This study examines RAP treated with various refined separation frequency parameters, analyzes the variation rules and the variability of RAP aggregate gradation, asphalt content, asphalt properties, and aggregate properties, and calculates the maximum mixing percentage of coarse RAP material by using the gradation variability control method and the asphalt content variability control method. The results show that the variability of gradation and asphalt content of coarsely separated RAP is considerable, and a refined separation process significantly reduces the variability of gradation and asphalt content of RAP; the agglomeration of RAP decreases with an increase in the refined separation frequency; and the RAP agglomeration of three kinds of RAPs (E1, E2, and E3) under a refined separation frequency of 55 Hz reduces by 6.40%, 4.30%, and 4.30%, respectively, as compared with that of coarsely separated RAPs. The asphalt content of the refined separation RAP gradually decreases with an increase in frequency, and the asphalt content of E1 and E2 (55 Hz) was only 0.95% and 1.10%, respectively. The maximum percentage of RAP in recycled asphalt mixtures was calculated using the gradation variability control method and the asphalt content variability control method, respectively. The maximum proportions of RAP were 45% and 33% for A1 (0 Hz), respectively, and the maximum proportions of RAP for E1 (55 Hz) were all 100%. The results of the two methods show that the process of refined separation can increase the maximum proportion of blended RAP materials. They also demonstrate that the refined separation process can increase the maximum blending ratio of coarse RAP materials, thereby improving the quality of the RAP, increasing the proportion of RAP blending, and ensuring the quality of the recycled asphalt mixture. In conclusion, the refined separation process holds promise for maximizing the potential value of RAP and optimizing its recycling, environmental, and economic benefits.

To study the microscale properties of the interface between cement emulsified asphalt and aged asphalt in the emulsified asphalt cold recycled mixture, the PeakForce quantitative nano mechanics (PF-QNM) technology of atomic force microscope (AFM) and dynamic shear rheological (DSR) test were used. The results showed that at the microscale, the cement products were spot-like distribution in the surface morphology of the composite system consisting of cement emulsified asphalt and aged asphalt. The approximate interface area could be distinguished by the distribution of the cement products. The gradient of the microscale mechanical properties between cement emulsified asphalt and aged asphalt could quantify the interface thickness. When the penetration of aged asphalt was less than 20 (0.1 mm) and the cement content was 1.0%–2.0%, the mechanical properties of interface were relatively close, and the theory of black aggregate was basically applicable. At the macroscale, the rheological property of the composite system had a good correlation with the microscale modulus of the interface, and a quantitative model of the correlation was proposed. When the penetration of aged asphalt was less than 30 (0.1 mm), the fatigue property of the composite system was relatively close under the same cement content, and the fatigue resistance of the composite system could be improved to a large extent by decreasing the cement content appropriately. Finally, to obtain a more general conclusion, it is necessary to choose a variety of emulsified asphalt and carry out a comparative analysis of different emulsified asphalt content.