The aim of this study was to evaluate the possible effects of a 50 Hz electric field on brain tissue and the positive effects of juglone (5-hydroxy-1,4-naphthoquinone) antioxidant activity, using the immunohistochemical technique on male Wistar-Albino rats. The effects on blood tissue were also examined using biochemical, physiological and comet methods. Animals were randomly divided into three groups (eight in each group): group I: control, group II: electric field, group III: 50 Hz electric field + juglone (5-hydroxy-1,4-naphthoquinone)/300 ppm. Juglone was applied per day by gavage over 30 days. At the end of the experimental procedure, animals were sacrificed and brain tissue was subjected to routine histologic and immunohistochemical processes. As a result of histophatological examination, the brain tissue of rats with 50 Hz electric field exposure showed severe histopathological changes. The differences between groups were statistically significant according to total comet score (p = 0.001). For the antioxidant parameters on the blood, SOD activity in the electric field group was significantly higher among the other groups, although we did not find significant differences in MDA, CAT activity level.

Although heavy metals are naturally occurring elements that are found throughout the Earth’s crust, most environmental contamination and human exposure result from anthropogenic activities, such as mining and smelting operations, industrial production and use, and the domestic and agricultural use of metals and metal-containing compounds. The accumulation of heavy metals eventually produces reactive oxygen species that can cause oxidative stress, which may lead to the production of various diseases. The aim of this study was to evaluate the possible effects of iron and zinc on kidney and liver tissues and the positive effects of juglone (5-hydroxy-1,4-naphthoquinone) antioxidant activity, using an immunohistochemical technique. The animals under study were randomly divided into five groups (seven in each group): group I, control; group II, iron (Fe) (600 ppm); group III, zinc (Zn) (400 ppm); group IV, Fe + antioxidant juglone; and group V, Zn + antioxidant juglone. Hematoxylin-eosin (H&E) was applied to determine the histological sides of the damage caused by the heavy metals in the liver and kidney tissues and the effects of the administration of juglone on reducing these damages. Furthermore, the immunohistochemical TUNEL method was applied to determine the DNA damages in the cells. The density of the damage in the liver and kidney tissues of the iron group was higher than in the other groups.

Numerous studies have demonstrated that the brain and blood–brain barrier, which are sensitive targets for cell phone and microwave radiation, are damaged after exposure. Additionally, ginseng has been shown to play a role in preserving the integrity of the blood–brain barrier. In this study, we investigated the immunohistochemical, genetic and biochemical effects of electromagnetic field (EMF) on the blood–brain barrier (BBB) and the protective role of ginseng on these effects. The animals were randomly allocated into seven groups (eight in each group): group I: control, group II: sham, group III: ginseng, group IV: 2100 MHz EMF, group V: 2100 MHz EMF + ginseng, group VI: 2450 MHz EMF, group VII: 2450 MHz EMF + ginseng. EMF groups exposed to EMF, 1 h day⁻¹ for 30 days. Ginseng was administered 150 mg/kg/day for 30 days. As a result, it was determined immunohistochemically that EMF caused apoptosis in brain tissue. It was observed that cyclooxygenase-2 (COX-2) gene decreased and B-cell lymphoma/leukemia-2 gene (BCL-2)-associated X (BAX) protein increased in EMF groups, as well as apoptosis formation. On the other hand, it was concluded that ginseng decreased the harmful effects by increasing the expression of the COX-2 gene and decreasing the BAX protein in this process leading to apoptosis.

Kınalı keklik (Alectoris chukar) ve sülün (Phasianus colchicus)’ün primer lenfoid organlardan bursa Fabricius ve timus dokularının histolojik yönden değerlendirilmesi ve karşılaştırılması amaçlandı. Çalışmada, Süleyman Demirel Üniversitesi Zootekni Bölümü Kanatlı Hayvan Yetiştirme Biriminden 3 adet, sağlıklı kınalı keklik ve sülün temin edildi. Bursa Fabricius ve timus doku örnekleri diseksiyon ile alındı. Genel histolojik incelemeler için hematoksilen-eosin ve masson trikrom boyamaları, doku ve hücrelerdeki nötral mukosubstansı ve karbonhidrat içeriğini belirleyebilmek için periodic acid schiff (PAS) reaksiyonu, plazma hücrelerini belirlemek için methyl green-pyronin boyaması uygulandı. Timusun major hücreleri için Weigert’s hematoksilenli van gieson boyaması gerçekleştirildi. Mast hücrelerini göstermek için ise toludin blue ve alcian blue/safranin O kombinasyon boyama teknikleri yapıldı. Kınalı keklik ve sülün timus ve bursa Fabricius’unun korteks ve medulla bölgelerindeki plazmosit, mastosit ve lenfosit hücrelerinin büyüklüklerinin ölçümleri yapılıp ortalama değerleri hesaplandı. Ayrıca dokuların fotoğrafları Leica DM 500 mikroskobunda çekildi. Kınalı keklik ve sülün bursa Fabricius ve timus dokuları histolojik açıdan karşılaştırıldığında önemli bir farklılığın olmadığı sonucuna varıldı.

There is strong scientific evidence that the electric field is harmful to life. Exposure to an electric field (EF) can cause lung toxicity and respiratory disorders. In addition, the electric field has been shown to cause tissue damage through inflammation and apoptosis. Juglone (JUG) is one of the powerful antioxidants with anti-apoptotic and anti-inflammatory, various pharmacological properties in the biological system. In this study, we evaluated the efficacy of JUG against the potential adverse effects of electric field on the lung. Twenty-four Wistar albino rats were randomly divided into three groups; control group (Cont), EF group, and EF exposure+JUG-treated group (EJUG). After routine histological procedures, sections stained with hematoxylin-eosin (H&E) showed significant changes in lung tissues in the EF group compared to the Cont group. Significant protective effects were observed in the building volumes and histopathology in the EJUG group. Our immunohistochemical and gene expression results increased the expression of caspase-3 and tumor necrosis factor alpha (TNF-alpha) in the EF group (p < 0.05). Juglon increased cytokine signal suppressor (SOCS) expression (p < 0.001). These findings were consistent with the antioxidant effect of JUG treatment. We reasoned that exposure to EF damaged rat lung tissues and administration of JUG alleviated the complications caused by 50 Hz EF.

Amaç Lokal ve sistemik faktörlerin, östrojen reseptörü pozitif meme kanseri riskinde artış olan postmenopozal obez kadınlarda meme kanseri hücrelerinin büyümesini yönlendirdiği gösterilmiştir. Postmenapozal dönemdeki obez kadınlarda, dolaşımdaki girelin hormonunun azaldığı, bazı tümör hücreleri tarafından üretildiği ve ayrıca tümör büyümesini etkilediği gösterilmiştir. Bu çalışmada, adiponektin uygulamasının östrojen reseptörü pozitif MCF-7 meme kanseri hücre hattındaki rolünü ve girelin seviyeleri üzerine olan etkilerini araştırmayı amaçladık. Gereç ve Yöntem MCF-7 meme kanseri hücre hattı 2 boyutlu kültürde çoğaltıldı. MCF-7 meme kanseri hücrelerine artan konsantrasyonlarda adiponektin (50-5000 ng/mL) ile muamele edilerek inhibisyon dozu belirlendi. MCF-7 meme kanseri hücre hattına 50, 100, 250 ve 500 ng/ mL konsantrasyonda adiponektin uygulanarak 24, 48 ve 72. Saatlerde örneklerde girelin seviyeleri enzim bağlı immünosorbent analizi (ELISA) ile ölçüldü. Bulgular 24, 48 ve 72. saate alınan MCF-7 meme kanseri hücre hattı kültür örneklerinde 50, 100, 250 ve 500 ng/mL adiponektin konsantrasyonuna bağlı olarak proliferasyonun azaldığı bulundu. 24, 48 ve 72. saate alınan MCF-7 meme kanseri hücre hattı kültür örneklerinde 50, 100, 250 ve 500 ng/mL adiponektin konsantrasyonuna bağlı olarak girelin miktarında artış oluştuğu bulundu. Sonuç Çalışmamızda MCF-7 meme kanseri hücre hattında (ER/PR pozitif) anti-kanserojenik özelliği kanıtlanmış olan ve aynı zamanda obezite ile negatif korelasyon gösteren adiponektinin, girelin ile karşılıklı etkileşimlerinin, ER pozitif meme kanserlerindeki rolünü ortaya koymuş olduk.