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University Educator/Researcher
01 October 2003 - 30 August 2021
The lyoprotective effects of mannitol and lactose have been evaluated in the production of sildenafil citrate liposomes. Liposomes were prepared by mixing the components under ultrasonic agitation, followed by a transmembrane pH gradient for remote drug loading. Mannitol and lactose, as compared to sucrose and trehalose, were used as the stabilizing agents, and different freeze-drying cycles were assayed. The remaining moisture and the thermal characteristics of the lyophilized samples were analyzed. Size, entrapment efficiency, biocompatibility, and cell internalization of original and rehydrated liposomes were compared. The type of additive did not affect the biocompatibility or cell internalization, but did influence other liposome attributes, including the thermal characteristics and the remaining moisture of the lyophilized samples. A cut-off of 5% (w/w) remaining moisture was an indicator of primary drying completion—information useful for scaling up and transfer from laboratory to large-scale production. Lactose increased the glass transition temperature to over 70 °C, producing lyoprotective effects similar to those obtained with sucrose. Based on these results, formulations containing liposomes lyophilized with lactose meet the FDA’s requirements and can be used as a biocompatible and biodegradable vehicle for the pulmonary delivery of therapeutic doses of sildenafil citrate.
María De Jesús Valle; Andreía Alves; Paula Coutinho; Maximiano Prata Ribeiro; Cristina Maderuelo; Amparo Sánchez Navarro. Lyoprotective Effects of Mannitol and Lactose Compared to Sucrose and Trehalose: Sildenafil Citrate Liposomes as a Case Study. Pharmaceutics 2021, 13, 1164 .
AMA StyleMaría De Jesús Valle, Andreía Alves, Paula Coutinho, Maximiano Prata Ribeiro, Cristina Maderuelo, Amparo Sánchez Navarro. Lyoprotective Effects of Mannitol and Lactose Compared to Sucrose and Trehalose: Sildenafil Citrate Liposomes as a Case Study. Pharmaceutics. 2021; 13 (8):1164.
Chicago/Turabian StyleMaría De Jesús Valle; Andreía Alves; Paula Coutinho; Maximiano Prata Ribeiro; Cristina Maderuelo; Amparo Sánchez Navarro. 2021. "Lyoprotective Effects of Mannitol and Lactose Compared to Sucrose and Trehalose: Sildenafil Citrate Liposomes as a Case Study." Pharmaceutics 13, no. 8: 1164.
Solvent-free microwave extraction (SFME) is a combination of microwave heating and dry distillation performed at atmospheric pressure without the addition of water or organic solvents that has been proposed as a green method for the extraction of essential oils from aromatic and medicinal herbs. In this work, SFME and the conventional techniques of steam distillation (SD) and hydrodistillation (HD) were compared with respect to the extraction and antioxidant and antimicrobial activities of Thymus mastichina essential oil. The main constituent of essential oils obtained using different methods was 1,8-cineole (eucalyptol). The results showed that the essential oils extracted by means of SFME in 30 min were quantitatively (yield) and qualitatively (aromatic profile) similar to those obtained using conventional HD over 120 min. In addition, SFME generates less waste and less solvent, consumes less energy, and provides a higher yield for a shorter extraction time, which is advantageous for the extraction of the T. mastichina essential oil compared to SD. The antioxidant and antimicrobial activities of the T. mastichina essential oil obtained from either SFME or conventional extraction methods (SD or HD) showed a similar pattern. Large-scale experiments using this SFME procedure showed a potential industrial application.
André R. T. S. Araujo; Sandrine Périno; Xavier Fernandez; Cassandra Cunha; Márcio Rodrigues; Maximiano P. Ribeiro; Luisa Jordao; Lúcia A. Silva; Jesus Rodilla; Paula Coutinho; Farid Chemat. Solvent-Free Microwave Extraction of Thymus mastichina Essential Oil: Influence on Their Chemical Composition and on the Antioxidant and Antimicrobial Activities. Pharmaceuticals 2021, 14, 709 .
AMA StyleAndré R. T. S. Araujo, Sandrine Périno, Xavier Fernandez, Cassandra Cunha, Márcio Rodrigues, Maximiano P. Ribeiro, Luisa Jordao, Lúcia A. Silva, Jesus Rodilla, Paula Coutinho, Farid Chemat. Solvent-Free Microwave Extraction of Thymus mastichina Essential Oil: Influence on Their Chemical Composition and on the Antioxidant and Antimicrobial Activities. Pharmaceuticals. 2021; 14 (8):709.
Chicago/Turabian StyleAndré R. T. S. Araujo; Sandrine Périno; Xavier Fernandez; Cassandra Cunha; Márcio Rodrigues; Maximiano P. Ribeiro; Luisa Jordao; Lúcia A. Silva; Jesus Rodilla; Paula Coutinho; Farid Chemat. 2021. "Solvent-Free Microwave Extraction of Thymus mastichina Essential Oil: Influence on Their Chemical Composition and on the Antioxidant and Antimicrobial Activities." Pharmaceuticals 14, no. 8: 709.
The search for natural products with benefits for skin regeneration has gained a much broader interest worldwide. Microalgae represent a natural source of interesting bioactive compounds with an impact on wound-healing applications. The potential beneficial effects of microalgal carotenoids, polyunsaturated fatty acids, polysaccharides, and crude extracts have been reported in wound-healing applications due to their different biological activities, namely: i) the quenching of reactive oxygen species; ii) the suppression of the production of inflammatory cytokines and transduction cascades, and iii) the prevention of the microbial growth at the wound site. Wound dressings based on microalgae, and their bioactive compounds, which are aimed at being used in the treatment of skin injuries, are reviewed. Finally, the emerging strategies in the production of live microalgae-based photosynthetic scaffolds for skin regeneration are also outlined.
Sónia P. Miguel; Maximiano P. Ribeiro; Ana Otero; Paula Coutinho. Application of microalgae and microalgal bioactive compounds in skin regeneration. Algal Research 2021, 58, 102395 .
AMA StyleSónia P. Miguel, Maximiano P. Ribeiro, Ana Otero, Paula Coutinho. Application of microalgae and microalgal bioactive compounds in skin regeneration. Algal Research. 2021; 58 ():102395.
Chicago/Turabian StyleSónia P. Miguel; Maximiano P. Ribeiro; Ana Otero; Paula Coutinho. 2021. "Application of microalgae and microalgal bioactive compounds in skin regeneration." Algal Research 58, no. : 102395.
Introduction: In 2015, the EU(7)-PIM List was published, which identifies potentially inappropriate medicines in older patients and resulted from a consensus of experts from seven European countries. Portugal was not part of this group, so it was not originally adapted to the Portuguese reality. With this work, we intend to elaborate a list of potentially inappropriate medicines adapted to the reality of medicines marketed in Portugal, through the operationalization of the EU(7)-PIM List for the national reality and to evaluate the adequacy of its use for clinical practice.Material and Methods: Search, in INFARMED’s Infomed database, of drugs that are included in the EU(7)-PIM List that have marketing authorization, and analysis of possible new drugs for inclusion in the list. The tool adapted to the Portuguese reality was applied to a sample of 1089 outpatient, polymedicated older patients from 38 primary care units in Central Portugal.Results: The final PIM list adapted to the Portuguese reality includes 184 potentially inappropriate medicines (from these, 178 are active substances, five are classes of drugs, and one corresponds to the sliding scale therapeutic scheme used in insulin therapy). Of 1089 polymedicated older patients, 83.7% took at least one drug included in the final potentially inappropriate medicines list or belonging to one of the groups included in the list, and, on average, each patient took 1.74 (IQR 1 – 2).Discussion: Even though the availability of drugs on the market is quite diverse, the EU(7)-PIM List has been used in several European countries. With this study, we operationalized the European list for the Portuguese reality, which will enable its application in clinical practice.Conclusion: The list drawn up is a useful tool for the identification of potentially inappropriate medicines, easy to use in clinical practice and research.
Daniela A. Rodrigues; Maria T. Herdeiro; Petra A. Thürmann; Adolfo Figueiras; Paula Coutinho; Fátima Roque. Operacionalização para Portugal da Lista EU(7)-PIM para Identificação de Medicamentos Potencialmente Inapropriados nos Idosos. Acta Médica Portuguesa 2021, 34, 194 .
AMA StyleDaniela A. Rodrigues, Maria T. Herdeiro, Petra A. Thürmann, Adolfo Figueiras, Paula Coutinho, Fátima Roque. Operacionalização para Portugal da Lista EU(7)-PIM para Identificação de Medicamentos Potencialmente Inapropriados nos Idosos. Acta Médica Portuguesa. 2021; 34 (3):194.
Chicago/Turabian StyleDaniela A. Rodrigues; Maria T. Herdeiro; Petra A. Thürmann; Adolfo Figueiras; Paula Coutinho; Fátima Roque. 2021. "Operacionalização para Portugal da Lista EU(7)-PIM para Identificação de Medicamentos Potencialmente Inapropriados nos Idosos." Acta Médica Portuguesa 34, no. 3: 194.
The Jarmelista autochthonous bovine breed has a sustainable production and is part of the culture of the Portuguese territory, representing a touristic attraction and originating a differentiated beef product that can only be found in a particular region of the country. However rural and livestock population evolution in Portugal’s inland has demonstrated a great regression with consequences for environment and nature conservation. In this context and considering that silvopastoral activity has shaped the natural areas of mountain territories since its beginning, rethinking the importance of such activity has become vital for the territory sustainability. In this perspective, this work presents an analysis of the adaptation and evolution of Jarmelista bovine breed production to current times, perceiving its limitations, challenges, and success potential, supported by a data collection of secondary and primary sources. Despite the natural, healthy, and sustainable value of this particular bovine meat, we observed that is still not recognised by the market or even by the producers. The inability of proving the Jarmelista beef added value within the value chain is the main cause of businesses and consumers sceptic and disbelief in the potential of its economic and tourism contribution. Several possibilities and actions were identified to contradict this path.
Paula Coutinho; Manuel Simões; Carlos Pereira; Teresa Paiva. Sustainable Local Exploitation and Innovation on Meat Products Based on the Autochthonous Bovine Breed Jarmelista. Sustainability 2021, 13, 2515 .
AMA StylePaula Coutinho, Manuel Simões, Carlos Pereira, Teresa Paiva. Sustainable Local Exploitation and Innovation on Meat Products Based on the Autochthonous Bovine Breed Jarmelista. Sustainability. 2021; 13 (5):2515.
Chicago/Turabian StylePaula Coutinho; Manuel Simões; Carlos Pereira; Teresa Paiva. 2021. "Sustainable Local Exploitation and Innovation on Meat Products Based on the Autochthonous Bovine Breed Jarmelista." Sustainability 13, no. 5: 2515.
Thymus mastichina has the appearance of a semishrub and can be found in jungles and rocky lands of the Iberian Peninsula. This work aimed to review and gather available scientific information on the composition and biological properties of T. mastichina. The main constituents of T. mastichina essential oil are 1,8-cineole (or eucalyptol) and linalool, while the extracts are characterized by the presence of flavonoids, phenolic acids, and terpenes. The essential oil and extracts of T. mastichina have demonstrated a wide diversity of biological activities. They showed antibacterial activity against several bacteria such as Escherichia coli, Proteus mirabilis, Salmonella subsp., methicillin-resistant and methicillin-sensitive Staphylococcus aureus, Listeria monocytogenes EGD, Bacillus cereus, and Pseudomonas, among others, and antifungal activity against Candida spp. and Fusarium spp. Additionally, it has antioxidant activity, which has been evaluated through different methods. Furthermore, other activities have also been studied, such as anticancer, antiviral, insecticidal, repellent, anti-Alzheimer, and anti-inflammatory activity. In conclusion, considering the biological activities reported for the essential oil and extracts of T. mastichina, its potential as a preservative agent could be explored to be used in the food, cosmetic, or pharmaceutical industries.
Márcio Rodrigues; Ana Clara Lopes; Filipa Vaz; Melanie Filipe; Gilberto Alves; Maximiano P. Ribeiro; Paula Coutinho; André R. T. S. Araujo. Thymus mastichina: Composition and Biological Properties with a Focus on Antimicrobial Activity. Pharmaceuticals 2020, 13, 479 .
AMA StyleMárcio Rodrigues, Ana Clara Lopes, Filipa Vaz, Melanie Filipe, Gilberto Alves, Maximiano P. Ribeiro, Paula Coutinho, André R. T. S. Araujo. Thymus mastichina: Composition and Biological Properties with a Focus on Antimicrobial Activity. Pharmaceuticals. 2020; 13 (12):479.
Chicago/Turabian StyleMárcio Rodrigues; Ana Clara Lopes; Filipa Vaz; Melanie Filipe; Gilberto Alves; Maximiano P. Ribeiro; Paula Coutinho; André R. T. S. Araujo. 2020. "Thymus mastichina: Composition and Biological Properties with a Focus on Antimicrobial Activity." Pharmaceuticals 13, no. 12: 479.
Exopolysaccharides (EPS) produced by cyanobacteria are complex biomolecules of anionic nature with potential biomedical applications. In this study, the EPS produced by the Nostoc sp. strains PCC7936 and PCC7413 were characterized and evaluated as a biomaterial for new wound dressings. The addition of acetate ions to the culture medium slightly stimulated EPS production, achieving 1463.1 ± 16.0 mgL-1 (PCC7413) and 1372.1 ± 29.0 mgL-1 (PCC7936). Both EPS presented nine monosaccharide residues and a MW > 1000 kDa. The acetate addition changed the monosaccharide molar percentages. FTIR and DLS results confirmed the anionic nature and the presence of sulfate groups in both EPS, which are determinant features for biomedical applications. Both EPS at 1%(w/v) formed gels in the presence of 0.4%(w/v) FeCl3. Results obtained for MTT assay and wound healing in vitro scratch assay revealed hydrogels biocompatibility and ability to promote fibroblast migration and proliferation that was greater in PCC7936. The Nostoc EPS hydrogels presented promising properties to be applied in the treatment of skin injuries.
Xavier Alvarez; Andreia Alves; Maximiano P. Ribeiro; Massimo Lazzari; Paula Coutinho; Ana Otero. Biochemical characterization of Nostoc sp. exopolysaccharides and evaluation of potential use in wound healing. Carbohydrate Polymers 2020, 254, 117303 .
AMA StyleXavier Alvarez, Andreia Alves, Maximiano P. Ribeiro, Massimo Lazzari, Paula Coutinho, Ana Otero. Biochemical characterization of Nostoc sp. exopolysaccharides and evaluation of potential use in wound healing. Carbohydrate Polymers. 2020; 254 ():117303.
Chicago/Turabian StyleXavier Alvarez; Andreia Alves; Maximiano P. Ribeiro; Massimo Lazzari; Paula Coutinho; Ana Otero. 2020. "Biochemical characterization of Nostoc sp. exopolysaccharides and evaluation of potential use in wound healing." Carbohydrate Polymers 254, no. : 117303.
The competitiveness of the agro-industrial sector depends not only on its specific performance but also on the character and degree of the innovation performance, vital to added value development and differentiation in the biobased value-chain. This work intends to show, how through research and development (R&D), collaboration is possible to improve agri-food companies’ competitiveness, helping them to integrate biotechnology and offer innovative products. The method used to support the R&D collaboration model developed involves a diagnosis of biotechnological tools use, for developing appropriate solutions from food safety to food quality, improving health, and achieving new ingredients and/or food products within an agri-food Association partners survey results were integrated into the study of R&D collaboration practice. Results show that the companies (wine culture, fruticulture, and olive culture subsectors) inquired do not develop biotechnology research. They were all micro-business with a low volume of commercial billing, and only 27.3% claimed to have developed research activities in partnership with external research centres, but were not associated with higher education institutions. The barriers to the implementation of biotechnology techniques considered more relevant by respondents were access to capital and specialized human resources, which led to reinforcing the R&D collaboration strategy design.
Teresa Paiva; Maximiano Ribeiro; Paula Coutinho. R&D Collaboration, Competitiveness Development, and Open Innovation in R&D. Journal of Open Innovation: Technology, Market, and Complexity 2020, 6, 116 .
AMA StyleTeresa Paiva, Maximiano Ribeiro, Paula Coutinho. R&D Collaboration, Competitiveness Development, and Open Innovation in R&D. Journal of Open Innovation: Technology, Market, and Complexity. 2020; 6 (4):116.
Chicago/Turabian StyleTeresa Paiva; Maximiano Ribeiro; Paula Coutinho. 2020. "R&D Collaboration, Competitiveness Development, and Open Innovation in R&D." Journal of Open Innovation: Technology, Market, and Complexity 6, no. 4: 116.
Population ageing is one of the most significant social transformations of the twenty-first century. The increase in average life expectancy was a successful challenge achieved in the modern world. However, nowadays a new challenge arises for all society: achieving a better quality of life for increasing people’s life. The comorbidities associated with ageing make elderly prone to polypharmacy. On the other hand, physiological and cognitive changes interfere with drugs’ pharmacodynamics and pharmacokinetics contributing to drug-related problems which have been reported to account for a large percentage of emergency treatment and hospitalizations of older people, increasing the costs with health in the most aged regions. In order to reduce the use of potentially inappropriate medicines in this population, strategies and tools have been developed in recent years to assess the appropriateness medication use in the elderly.
Daniela A. Rodrigues; Maria Teresa Herdeiro; Adolfo Figueiras; Paula Coutinho; Fátima Roque. Elderly and Polypharmacy: Physiological and Cognitive Changes. Frailty in the Elderly - Physical, Cognitive and Emotional Domains [Working Title] 2020, 1 .
AMA StyleDaniela A. Rodrigues, Maria Teresa Herdeiro, Adolfo Figueiras, Paula Coutinho, Fátima Roque. Elderly and Polypharmacy: Physiological and Cognitive Changes. Frailty in the Elderly - Physical, Cognitive and Emotional Domains [Working Title]. 2020; ():1.
Chicago/Turabian StyleDaniela A. Rodrigues; Maria Teresa Herdeiro; Adolfo Figueiras; Paula Coutinho; Fátima Roque. 2020. "Elderly and Polypharmacy: Physiological and Cognitive Changes." Frailty in the Elderly - Physical, Cognitive and Emotional Domains [Working Title] , no. : 1.
Hydrogels are considered to be the most ideal materials for the production of wound dressings since they display a three-dimensional structure that mimics the native extracellular matrix of skin as well as a high-water content, which confers a moist environment at the wound site. Until now, different polymers have been used, alone or blended, for the production of hydrogels aimed for this biomedical application. From the best of our knowledge, the application of a xanthan gum–konjac glucomannan blend has not been used for the production of wound dressings. Herein, a thermo-reversible hydrogel composed of xanthan gum–konjac glucomannan (at different concentrations (1% and 2% w/v) and ratios (50/50 and 60/40)) was produced and characterized. The obtained data emphasize the excellent physicochemical and biological properties of the produced hydrogels, which are suitable for their future application as wound dressings.
Andreia Alves; Sónia P. Miguel; André R.T.S. Araujo; María José De Jesús Valle; Amparo Sánchez Navarro; Ilídio J. Correia; Maximiano P. Ribeiro; Paula Coutinho. Xanthan Gum–Konjac Glucomannan Blend Hydrogel for Wound Healing. Polymers 2020, 12, 99 .
AMA StyleAndreia Alves, Sónia P. Miguel, André R.T.S. Araujo, María José De Jesús Valle, Amparo Sánchez Navarro, Ilídio J. Correia, Maximiano P. Ribeiro, Paula Coutinho. Xanthan Gum–Konjac Glucomannan Blend Hydrogel for Wound Healing. Polymers. 2020; 12 (1):99.
Chicago/Turabian StyleAndreia Alves; Sónia P. Miguel; André R.T.S. Araujo; María José De Jesús Valle; Amparo Sánchez Navarro; Ilídio J. Correia; Maximiano P. Ribeiro; Paula Coutinho. 2020. "Xanthan Gum–Konjac Glucomannan Blend Hydrogel for Wound Healing." Polymers 12, no. 1: 99.
The nutritional value of the marine cryptophyte Rhodomonas lens for the filter feeder Brachionus plicatilis as well as its biotechnological potential as a source of phycoerythrin (PE) and polyunsaturated fatty acids (PUFA) were evaluated in semi-continuous cultures maintained with different daily renewal rates (RR), from 10% (R10) to 50% (R50) of the total volume. Steady-state cell density decreased from 22 to 7 × 106 cells mL−1 with increasing RR, with the maximum cell productivity, nearly 0.4 g L−1 day−1, observed with R40. PE cell content attained the highest values with the highest RR (circa 9 pg cell−1). All treatments of R. lens maintained under nitrate-saturated conditions (R20-R50) showed a similar high content of PUFAs, > 60% of total fatty acids (FA), with linolenic acid (18:3n-3) and 18:4n-3, representing 12 and 29% of total FA respectively. The PUFA level in the nitrogen-limited R10 cultures was significantly lower (37%). R. lens promoted higher weight gain in the rotifer B. plicatilis than Tisochrysis lutea (T-ISO), a species commonly used for rotifer culture and enrichment. Significant differences were found in the protein content and in the ratio n-3/n-6 fatty acids among rotifers fed with R. lens from different RRs, with higher values being found in those fed with R. lens from higher RRs. The enrichment of the rotifers for short periods of 3 h was sufficient to modify the biochemical composition of the rotifers, but it was evidenced as too short for the accumulation of PUFAs, when compared to long-term (24 h) enrichment. The rotifers reflected the higher protein and PUFA content of R. lens cultivated with nutrient sufficient microalgae (R40) after only 3 h of enrichment. These results demonstrate that semi-continuous culture of R. lens under appropriate conditions can strongly enhance the nutritional value of this species, being reflected in the growth and biochemical composition of the filter feeder, even in short exposure periods.
Paula Coutinho; Martiña Ferreira; Isabel Freire; Ana Otero. Enriching Rotifers with “Premium” Microalgae: Rhodomonas lens. Marine Biotechnology 2019, 22, 118 -129.
AMA StylePaula Coutinho, Martiña Ferreira, Isabel Freire, Ana Otero. Enriching Rotifers with “Premium” Microalgae: Rhodomonas lens. Marine Biotechnology. 2019; 22 (1):118-129.
Chicago/Turabian StylePaula Coutinho; Martiña Ferreira; Isabel Freire; Ana Otero. 2019. "Enriching Rotifers with “Premium” Microalgae: Rhodomonas lens." Marine Biotechnology 22, no. 1: 118-129.
Development of cosmetic formulations to provide a controlled release of hydrophilic active compounds from mineral medicinal waters constitutes an attractive challenge. The objective of this study was the development and the characterization of a dermocosmetic gel formulation with Cró thermal water, from Beira Interior of Portugal, as a major functional ingredient. Concentrations of mineral chemical elements of Cró thermal water were previously determined by inductively coupled plasma-optical emission spectrometry and cytotoxicity assays using thermal water were carried out on normal human dermal fibroblasts (NHDF) cells. Then, the Cró thermal water was included (95%) in a developed gel formulation that was characterized through rheological and texture analysis and submitted to stability assays during 30 days. The effects on the skin volunteers, namely skin pH, the degree of hydration, transepidermal water loss and skin relief, were evaluated through non-invasive biometric techniques. A gel formulation including purified water as used as control. Cró thermal water is rich on several chemical elements in particular sodium, silica, potassium and calcium besides some trace elements, with important functions for the skin. NHDF cells adhered and proliferated in the presence of thermal water confirming the biocompatibility of the major component of the gel formulation. The developed gel formulation based on thermal water resulted in an improvement of textural parameters, comparing with the purified water-based one. Significant improvements in the cutaneous biometric parameters (degree of hydration, transepidermal water loss and skin relief) of volunteers were also registered for the gel formulation containing thermal water. This study demonstrated for the first time the potential benefits of Cró thermal water in a gel formulation to be used in cosmetic and dermatological applications.
Fábio Nunes; Márcio Rodrigues; Maximiano Ribeiro; Elena Ugazio; Roberta Cavalli; Ornella Abollino; Paula Coutinho; A. R. T. S. Araujo. Incorporation of Cró thermal water in a dermocosmetic formulation: cytotoxicity effects, characterization and stability studies and efficacy evaluation. International Journal of Cosmetic Science 2019, 41, 604 -612.
AMA StyleFábio Nunes, Márcio Rodrigues, Maximiano Ribeiro, Elena Ugazio, Roberta Cavalli, Ornella Abollino, Paula Coutinho, A. R. T. S. Araujo. Incorporation of Cró thermal water in a dermocosmetic formulation: cytotoxicity effects, characterization and stability studies and efficacy evaluation. International Journal of Cosmetic Science. 2019; 41 (6):604-612.
Chicago/Turabian StyleFábio Nunes; Márcio Rodrigues; Maximiano Ribeiro; Elena Ugazio; Roberta Cavalli; Ornella Abollino; Paula Coutinho; A. R. T. S. Araujo. 2019. "Incorporation of Cró thermal water in a dermocosmetic formulation: cytotoxicity effects, characterization and stability studies and efficacy evaluation." International Journal of Cosmetic Science 41, no. 6: 604-612.
The present work deals with the rational design and in vitro evaluation of vaginal tablets for focal delivery of fluconazole (FLZ) and itraconazol (ITZ). Drug loaded liposomes with and without d-alpha-tocopheryl polyethylene glycol 1000 succinate (vit E TPGS) were prepared by direct sonication of the components and mixed with albumin to obtain albusomes. Tablets were obtained by direct compression of the lyophilized cake. The influence of vit E TPGS on size, zeta potential and entrapment efficiency (EE%) of liposomes and albusomes was evaluated. Tablet swelling and drug release were studied by in vitro assays. Vit E TPGS neither affected the zeta potential nor the EE% of liposomes and albusomes, but affected the liposomes size and the tablet disintegration time. A rapid erosion was observed for the tablets with the highest content of vitamin, while a slow swelling for those lacking the vitamin (swelling index = 57.76 ± 13.51%). A faster drug release profile was obtained for the former compared to the latter. The in vitro assay showed that FLZ diffused and solved in the vaginal fluid simulant while ITZ remained into the albusomes, which slowly released ITZ-albumin complex and ITZ-loaded liposomes, both suitable carriers for drug transport to deeper vaginal endothelium.
Maria José De Jesús Valle; Paula Coutinho; Maximiano Prata Ribeiro; Amparo Sánchez Navarro. Lyophilized tablets for focal delivery of fluconazole and itraconazole through vaginal mucosa, rational design and in vitro evaluation. European Journal of Pharmaceutical Sciences 2018, 122, 144 -151.
AMA StyleMaria José De Jesús Valle, Paula Coutinho, Maximiano Prata Ribeiro, Amparo Sánchez Navarro. Lyophilized tablets for focal delivery of fluconazole and itraconazole through vaginal mucosa, rational design and in vitro evaluation. European Journal of Pharmaceutical Sciences. 2018; 122 ():144-151.
Chicago/Turabian StyleMaria José De Jesús Valle; Paula Coutinho; Maximiano Prata Ribeiro; Amparo Sánchez Navarro. 2018. "Lyophilized tablets for focal delivery of fluconazole and itraconazole through vaginal mucosa, rational design and in vitro evaluation." European Journal of Pharmaceutical Sciences 122, no. : 144-151.
Paula Coutinho; Carlos Pereira; Marcio Rodrigues; Paulo Poco; Ana Carreira; Joao Pedro Borges; Ana Soeiro; Teresa Paiva. Jarmelo Value – a pilot study of livestock production and territorial eco valorisation. Journal of Biotechnology 2018, 280, S42 .
AMA StylePaula Coutinho, Carlos Pereira, Marcio Rodrigues, Paulo Poco, Ana Carreira, Joao Pedro Borges, Ana Soeiro, Teresa Paiva. Jarmelo Value – a pilot study of livestock production and territorial eco valorisation. Journal of Biotechnology. 2018; 280 ():S42.
Chicago/Turabian StylePaula Coutinho; Carlos Pereira; Marcio Rodrigues; Paulo Poco; Ana Carreira; Joao Pedro Borges; Ana Soeiro; Teresa Paiva. 2018. "Jarmelo Value – a pilot study of livestock production and territorial eco valorisation." Journal of Biotechnology 280, no. : S42.
Andreia Isabel Moreira Alves; Maximiano P. Ribeiro; Paula Coutinho. New approaches to the development of wound dressing based on Xanthan Gum and Konjac Glucomannan. Journal of Biotechnology 2018, 280, S23 -S24.
AMA StyleAndreia Isabel Moreira Alves, Maximiano P. Ribeiro, Paula Coutinho. New approaches to the development of wound dressing based on Xanthan Gum and Konjac Glucomannan. Journal of Biotechnology. 2018; 280 ():S23-S24.
Chicago/Turabian StyleAndreia Isabel Moreira Alves; Maximiano P. Ribeiro; Paula Coutinho. 2018. "New approaches to the development of wound dressing based on Xanthan Gum and Konjac Glucomannan." Journal of Biotechnology 280, no. : S23-S24.
Paula Coutinho; Carina Pissarra; Andreia Isabel Moreira Alves; Andre R.T.S. Araújo; Maximiano P. Ribeiro. Postharvest quality of coated cherries cv. ‘Smith’ with alginate, chitosan and konjac glucomannan. Journal of Biotechnology 2018, 280, S51 .
AMA StylePaula Coutinho, Carina Pissarra, Andreia Isabel Moreira Alves, Andre R.T.S. Araújo, Maximiano P. Ribeiro. Postharvest quality of coated cherries cv. ‘Smith’ with alginate, chitosan and konjac glucomannan. Journal of Biotechnology. 2018; 280 ():S51.
Chicago/Turabian StylePaula Coutinho; Carina Pissarra; Andreia Isabel Moreira Alves; Andre R.T.S. Araújo; Maximiano P. Ribeiro. 2018. "Postharvest quality of coated cherries cv. ‘Smith’ with alginate, chitosan and konjac glucomannan." Journal of Biotechnology 280, no. : S51.
Skin wounds have significant morbidity and mortality rates associated. This is explained by the limited effectiveness of the currently available treatments, which in some cases do not allow the reestablishment of the structure and functions of the damaged skin, leading to wound infection and dehydration. These drawbacks may have an impact on the healing process and ultimately prompt patients’ death. For this reason, researchers are currently developing new wound dressings that enhance skin regeneration. Among them, electrospun polymeric nanofibres have been regarded as promising tools for improving skin regeneration due to their structural similarity with the extracellular matrix of normal skin, capacity to promote cell growth and proliferation and bactericidal activity as well as suitability to deliver bioactive molecules to the wound site. In this review, an overview of the recent studies concerning the production and evaluation of electrospun polymeric nanofibrous membranes for skin regenerative purposes is provided. Moreover, the current challenges and future perspectives of electrospun nanofibrous membranes suitable for this biomedical application are highlighted.
Sónia Alexandra Pereira Miguel; Daniela R. Figueira; Déborah Simões; Maximiano Ribeiro; Paula Coutinho; Paula Ferreira; Ilídio J. Correia. Electrospun polymeric nanofibres as wound dressings: A review. Colloids and Surfaces B: Biointerfaces 2018, 169, 60 -71.
AMA StyleSónia Alexandra Pereira Miguel, Daniela R. Figueira, Déborah Simões, Maximiano Ribeiro, Paula Coutinho, Paula Ferreira, Ilídio J. Correia. Electrospun polymeric nanofibres as wound dressings: A review. Colloids and Surfaces B: Biointerfaces. 2018; 169 ():60-71.
Chicago/Turabian StyleSónia Alexandra Pereira Miguel; Daniela R. Figueira; Déborah Simões; Maximiano Ribeiro; Paula Coutinho; Paula Ferreira; Ilídio J. Correia. 2018. "Electrospun polymeric nanofibres as wound dressings: A review." Colloids and Surfaces B: Biointerfaces 169, no. : 60-71.
Skin and soft tissue infections (SSTIs) have high rates of morbidity and mortality associated. Despite the successful treatment of some SSTIs, those affecting the subcutaneous tissue, fascia, or muscle delay the healing process and can lead to life-threatening conditions. Therefore, more effective treatments are required to deal with such pathological situations. Recently, wound dressings loaded with antimicrobial agents emerged as viable options to reduce wound bacterial colonization and infection, in order to improve the healing process. In this review, an overview of the most prominent antibacterial agents incorporated in wound dressings along with their mode of action is provided. Furthermore, the recent advances in the therapeutic approaches used in the clinic and some future perspectives regarding antibacterial wound dressings are also discussed.
Déborah Simões; Sónia Alexandra Pereira Miguel; Maximiano Ribeiro; Paula Coutinho; António Mendonça; Ilídio J. Correia. Recent advances on antimicrobial wound dressing: A review. European Journal of Pharmaceutics and Biopharmaceutics 2018, 127, 130 -141.
AMA StyleDéborah Simões, Sónia Alexandra Pereira Miguel, Maximiano Ribeiro, Paula Coutinho, António Mendonça, Ilídio J. Correia. Recent advances on antimicrobial wound dressing: A review. European Journal of Pharmaceutics and Biopharmaceutics. 2018; 127 ():130-141.
Chicago/Turabian StyleDéborah Simões; Sónia Alexandra Pereira Miguel; Maximiano Ribeiro; Paula Coutinho; António Mendonça; Ilídio J. Correia. 2018. "Recent advances on antimicrobial wound dressing: A review." European Journal of Pharmaceutics and Biopharmaceutics 127, no. : 130-141.
A novel high-performance liquid chromatography-diode array detection method based on microextraction by packed sorbent as a sample preparation approach was developed and validated for the determination of zonisamide in human plasma.
Dina Lourenço; Mafalda Sarraguça; Gilberto Alves; Paula Coutinho; André R. T. S. Araujo; Márcio José De Abreu Marques Rodrigues. A novel HPLC method for the determination of zonisamide in human plasma using microextraction by packed sorbent optimised by experimental design. Analytical Methods 2017, 9, 5910 -5919.
AMA StyleDina Lourenço, Mafalda Sarraguça, Gilberto Alves, Paula Coutinho, André R. T. S. Araujo, Márcio José De Abreu Marques Rodrigues. A novel HPLC method for the determination of zonisamide in human plasma using microextraction by packed sorbent optimised by experimental design. Analytical Methods. 2017; 9 (40):5910-5919.
Chicago/Turabian StyleDina Lourenço; Mafalda Sarraguça; Gilberto Alves; Paula Coutinho; André R. T. S. Araujo; Márcio José De Abreu Marques Rodrigues. 2017. "A novel HPLC method for the determination of zonisamide in human plasma using microextraction by packed sorbent optimised by experimental design." Analytical Methods 9, no. 40: 5910-5919.
Paula Barjona Do Nascimento Coutinho; Christine Friedl; Rainer Buchholz; Stephanie Christine Stute. Chemical regulation of Fea1 driven transgene expression in Chlamydomonas reinhardtii. Algal Research 2017, 26, 323 -329.
AMA StylePaula Barjona Do Nascimento Coutinho, Christine Friedl, Rainer Buchholz, Stephanie Christine Stute. Chemical regulation of Fea1 driven transgene expression in Chlamydomonas reinhardtii. Algal Research. 2017; 26 ():323-329.
Chicago/Turabian StylePaula Barjona Do Nascimento Coutinho; Christine Friedl; Rainer Buchholz; Stephanie Christine Stute. 2017. "Chemical regulation of Fea1 driven transgene expression in Chlamydomonas reinhardtii." Algal Research 26, no. : 323-329.