The number of tropical species established in the Mediterranean Sea has risen at an unprecedented rate in the last 40–50 years, often entering the basin from the Red Sea and to a lesser extent by shipping or mariculture. The taxonomic identity of these alien species can be difficult to determine and DNA barcoding can help to clarify the status of questionable species. One of these cases is the W-shaped mark Aglajidae slug usually identified as being the Indo-West Pacific species Chelidonura fulvipunctata Baba, 1938 but was first described in the Mediterranean Sea as a native species under the name C. mediterranea Swennen, 1961. A Bayesian phylogeny using the barcode marker cytochrome c oxidase subunit I and an ABGD species delimitation analysis unequivocally showed that specimens from the eastern and central Mediterranean Sea are conspecific with specimens from the Indian and Pacific Oceans. In this work, we hypothesize that C. fulvipunctata (presently occurring in the entire Mediterranean Sea and Indo-West Pacific; only once recorded in the Red Sea) has entered the Red Sea through the Mediterranean Sea. Thus the Red Sea can also be a receiver of tropical species that have arrived in the Mediterranean by other routes than Lessepsian immigration, with the Suez Canal acting as a “revolving door” allowing both species of Indo-Pacific origin to enter the Mediterranean and species established in the Mediterranean resilient to tropical/subtropical environmental conditions to move into the Red Sea

One of the aspects considered about the presence of contaminants in the aquatic ecosystems is their possible effect on critically endangered species, as the case of European eel, Anguilla anguilla. However, there is a lack of analytical methods to determine these contaminants due to the complexity of eel matrix (contains 5–20 % of lipids and 5–15 % of proteins). Thus, a multi-residue method using QuEChERS extraction a clean-up based on new specific sorbents (to eliminate lipids) and liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed to determine a mix of 21 contaminants. Compared to the previously reported methods (Degani et al., 1986), which were developed for mussels, in this study, one of the proposed extraction methods were adapted to different fish tissues of higher complexity, such as liver and muscle of A. anguilla. • The effectivity of dispersive solid phase extraction (dSPE) using new specific Enhanced Matrix Removal (EMR-lipid) as clean-up for lipid removal was tested. • Clean extracts of matrices with high protein (5–15 %) and lipid (5–20 %) content were obtained ensuring robustness and durability of the analytical systems. • Emerging contaminants extractable by this procedure comprise four different families (pesticides, perfluoroalkyl substances (PFASs), pharmaceuticals and drugs of abuse). Then, it could be further applied to wide scope screening strategies.

A census of the “opisthobranch” fauna in the Faro Lake and connected canals (NE Sicily) has been realized by photo documentation and specimen samplings, carried out monthly from February 2010 to the present. A total of 47 species have been reported, nine of which are cited in the historic and recent literature (from 1969 to 2016) vs. 38 first records; six were non-indigenous species. A major number of species, 16, was found exclusively in the lake with respect to the four species localized in the canals, whilst 10 were shared species. Based on the frequency of records, 24 “occasional”, 20 “settled” and three “invasive” species have been distinguished; these latter included two non-indigenous species, Aplysia dactylomela and Bursatella leachii, plus the Mediterranean Dendrodoris limbata, whose recent introduction in the Faro Lake from other Mediterranean lagoons is here postulated.

This study reports the returning of the seahorse in the Faro Lake and the most recent finding in a brackish coastal Mediterranean lagoon. After about 50 years from the first documented occurrence of Hippocampus hippocampus (Linnaeus, 1758) within the “Capo Peloro Lagoons”, North East Sicily, two adult specimens were observed alive in 2015. During that time, until the present rediscovery, the species was not recorded in biological studies and censuses for the area. This paper increases the knowledge about both the threatened seahorse species and the Natural Oriented Reserve of Capo Peloro.

According to UNEP and FAO reports, the fishing nets abandoned in the seas around the world represent approximately 10% of the plastic waste present in the oceans. These nets, also called ghost nets, can be degraded over time, releasing microplastics and contaminating the environment. Studying the presence and amount of microplastics in an area impacted by ghost nets in the Gulf of Cefalù, northern coast of Sicily, Italy can help to understand the level of contamination in these zones. The planned study methodology has been carried out by sampling sediment and rock substrate, on a quantitative basis, by scuba diving at seven stations selected as the most representative for the presence or absence of fishing nets in the study area. Two different extraction methods for sediment and rock samples were taken from the literature and modified for the present study. Microplastics determination was carried out according to, first, a visual identification and, second, a polymer type identification by ATR-FTIR spectroscopy, demonstrating the presence of polyamide, nylon, or polyethylene in the impacted areas, probably derived from the degradation of fishing nets. The present study reports the first record of microplastics determination in rock substrate samples recollected by scuba diving activities.