The design and development of a compact square-wave pulse generator for the electroporation of biological cells is presented. This electroporator can generate square-wave pulses with durations from 3 μs up to 10 ms, voltage amplitudes up to 3500 V, and currents up to 250 A. The quantity of the accumulated energy is optimized by means of a variable capacitor bank. The pulse forming unit design uses a crowbar circuit, which gives better control of the pulse form and its duration, independent of the load impedance. In such cases, the square-wave pulse form ensures better control of electroporation efficiency by choosing parameters determined in advance. The device has an integrated graphic LCD screen and measurement modules for the visualization of the current pulse, allowing for express control of the electroporation quality and does not require an external oscilloscope for current pulse recording. This electroporator was tested on suspensions of Saccharomyces cerevisiae yeast cells, during which, it was demonstrated that the application of such square-wave pulses ensured better control of the electroporation efficiency and cell viability after treatment using the pulsed electric field (PEF).

The thyristor-controlled reactor (TCR) compensator for smooth asymmetric compensation of reactive power in a low-voltage utility grid is proposed in this work. Two different topologies of compensator were investigated: topology based on a single-cored three-phase reactor and topology with separate reactors for every phase. The investigation of the proposed TCR compensator was performed experimentally using a developed experimental test bench for 12 kVAr total reactive power. The obtained results show that employment of separate reactors for every phase allows us to control the reactive power in every phase independently, and that the TCR compensator with three single-phase reactors is suitable for smooth and asymmetric compensation of reactive power in a low-voltage utility grid.

A measurement system based on the colossal magnetoresistance CMR-B-scalar sensor was developed for the measurement of short-duration high-amplitude magnetic fields. The system consists of a magnetic field sensor made from thin nanostructured manganite film with minimized memory effect, and a magnetic field recording module. The memory effect of the La_1−xSr_x(Mn_1−yCo_y)_zO₃ manganite films doped with different amounts of Co and Mn was investigated by measuring the magnetoresistance (MR) and resistance relaxation in pulsed magnetic fields up to 20 T in the temperature range of 80–365 K. It was found that for low-temperature applications, films doped with Co (LSMCO) are preferable due to the minimized magnetic memory effect at these temperatures, compared with LSMO films without Co. For applications at temperatures higher than room temperature, nanostructured manganite LSMO films with increased Mn content above the stoichiometric level have to be used. These films do not exhibit magnetic memory effects and have higher MR values. To avoid parasitic signal due to electromotive forces appearing in the transmission line of the sensor during measurement of short-pulsed magnetic fields, a bipolar-pulsed voltage supply for the sensor was used. For signal recording, a measurement module consisting of a pulsed voltage generator with a frequency up to 12.5 MHz, a 16-bit ADC with a sampling rate of 25 MHz, and a microprocessor was proposed. The circuit of the measurement module was shielded against low- and high-frequency electromagnetic noise, and the recorded signal was transmitted to a personal computer using a fiber optic link. The system was tested using magnetic field generators, generating magnetic fields with pulse durations ranging from 3 to 20 μs. The developed magnetic field measurement system can be used for the measurement of high-pulsed magnetic fields with pulse durations in the order of microseconds in different fields of science and industry.

This study presents a system for precisely measuring pulsed magnetic fields with high amplitude and microsecond duration with minimal interference. The system comprises a probe with an advanced magnetic field sensor and a measurement unit for signal conversion, analysis, and digitization. The sensor uses a thin nanostructured manganite La-Sr-Mn-O film exhibiting colossal magnetoresistance, which enables precise magnetic field measurement independent of its orientation. Films with different compositions were optimized and tested in pulsed magnetic fields. The measurement unit includes a pulsed voltage generator, an ADC, a microcontroller, and an amplifier unit. Two versions of the measurement unit were developed: one with a separate amplifier unit configured for the sensor positioned more than 1 m away from the measurement unit, and the other with an integrated amplifier for the sensor positioned at a distance of less than 0.5 m. A bipolar pulsed voltage supplying the sensor minimized the parasitic effects of the electromotive force induced in the probe circuit. The data were transmitted via a fiber optic cable to a PC equipped with a special software for processing and recording. Tests with 20–30 μs pulses up to 15 T confirmed the effectiveness of the system for measuring high pulsed magnetic fields.

The developed stepless compensation system of reactive power based on the thyristor controlled reactors is presented. The investigation results of the system obtained using simulation and experimental investigation are given.

The developed high power microsecond pulse generator (U = 50– 3500 V, I < 250 A, t = 3 μs–1 ms) based on the push-pull inverter has been presented. The generator is developed for the electroporation of biological cells. The high power microsecond pulse generator was tested using simulation and experimental investigation.