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Prof. Marcello Locatelli
University "G.d'Annunzio" Chieti-Pescara - Department of Pharmacy - Italy

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0 Bioequivalence
0 HPLC-MS
0 Pharmacokinetics
0 Urine
0 extraction

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HPLC
extracts
extraction
plasma
Urine
HPLC-DAD
whole blood
HPLC-MS
Pharmacokinetics
natural product
biological matrices
Bioequivalence
Method Development and Validation

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Journal article
Published: 30 August 2021 in Analytica
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An authenticity study on Italian grape marc spirit was carried out by gas chromatography (GC) and chemometrics. A grape marc spirit produced in Italy takes the particular name of “grappa”, a product which has peculiar tradition and production in its country of origin. Therefore, the evaluation of its authenticity plays an important role for its consumption in Italy, as well as for its exportation all around the world. For the present work, 123 samples of grappa and several kinds of spirits were analyzed in their alcohol content by electronic densimetry, and in their volatile fraction by gas-chromatography with a flame-ionization detector. Part of these samples (94) was employed as a training set to compute a chemometric model (by linear discriminant analysis, LDA) and the other part (29 samples) was used as a test set to validate it. Finally, two grappa samples seized from the market by the Italian Customs and Monopolies Agency and considered suspicious due to their aroma reported as non-compliant were projected onto the LDA model to evaluate the compliance with the “grappa” class. A further one-class classification method by principal component analysis (PCA) was carried out to evaluate the compliance with other classes. Results showed that the suspicious samples were not recognized as belonging to any of the analyzed spirit classes, confirming the starting hypothesis that they could be grappa samples adulterated in some way.

ACS Style

Silvia Arduini; Alessandro Zappi; Marcello Locatelli; Salvatore Sgrò; Dora Melucci. An Authentication Study on Grappa Spirit: The Use of Chemometrics to Detect a Food Fraud. Analytica 2021, 2, 84 -92.

AMA Style

Silvia Arduini, Alessandro Zappi, Marcello Locatelli, Salvatore Sgrò, Dora Melucci. An Authentication Study on Grappa Spirit: The Use of Chemometrics to Detect a Food Fraud. Analytica. 2021; 2 (3):84-92.

Chicago/Turabian Style

Silvia Arduini; Alessandro Zappi; Marcello Locatelli; Salvatore Sgrò; Dora Melucci. 2021. "An Authentication Study on Grappa Spirit: The Use of Chemometrics to Detect a Food Fraud." Analytica 2, no. 3: 84-92.

Journal article
Published: 03 August 2021 in Analytica
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The present paper reports a new application: the voltammetric determination in herbal medicines of ultra-trace thallium(I), in the presence of lead(II), tin(II) and antimony(III) as interfering metals, by square wave anodic stripping voltammetry (SWASV). A conventional three-electrodes voltammetric cell was employed with a stationary hanging mercury drop electrode (HMDE) as working electrode and a platinum electrode and an Ag|AgCl|KClsatd. electrode as auxiliary and reference electrodes, respectively. The supporting electrolyte was 0.5 mol L−1 ammonium citrate buffer pH 6.5 + 7.3·10−3 mol L−1 EDTA-Na2. The analytical procedure was verified by the analysis of the standard reference materials: spinach Leaves NIST-SRM 1570a and tomato leaves NIST-SRM 1573a from National Institute of Standards and Technology. Precision and trueness, expressed as relative standard deviation and relative error, respectively, were generally lower than 7% in all cases. Once set up on the standard reference materials, the analytical procedure was transferred and applied to herbal medicines samples—Taraxacun officinale weber, Eucalyptus globulus and Harpagophytum procumbens DC—sold on the market. A critical comparison with spectroscopic measurements is also discussed.

ACS Style

Dora Melucci; Sonia Casolari; Marcello Locatelli; Clinio Locatelli. Thallium: A Polluting Metal of New Generation. Its Voltammetric Determination in Herbal Medicines in Presence of Metal Interferences. Analytica 2021, 2, 76 -83.

AMA Style

Dora Melucci, Sonia Casolari, Marcello Locatelli, Clinio Locatelli. Thallium: A Polluting Metal of New Generation. Its Voltammetric Determination in Herbal Medicines in Presence of Metal Interferences. Analytica. 2021; 2 (3):76-83.

Chicago/Turabian Style

Dora Melucci; Sonia Casolari; Marcello Locatelli; Clinio Locatelli. 2021. "Thallium: A Polluting Metal of New Generation. Its Voltammetric Determination in Herbal Medicines in Presence of Metal Interferences." Analytica 2, no. 3: 76-83.

Review
Published: 29 June 2021 in Pharmaceuticals
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Therapeutic drug monitoring (TDM) of antiseizure medications (ASMs) represents a valuable tool to establish an appropriate patient therapy, to collect important information about drugs’ interactions and to evaluate patient’s metabolic capabilities. In recent years, a new volumetric absorptive microsampling technique using VAMS® technology and Mitra® devices, consisting of a sampling technique for the collection of fixed-volume capillary blood, was developed. These new devices provide a new home-sampling technique for whole blood that has been spread out to simplify sample collection from finger-pricks. This review is aimed to compare published articles concerning the application of VAMS® in epilepsy and to identify the strengths and improvement points for the TDM of antiseizure medications. VAMS® allowed a minimally invasive blood sampling even in the absence of trained personnel. Good stability data have indicated that storage and delivery can be facilitated only for specific ASMs. Trueness and precision parameters have been evaluated, and the hematocrit (HCT) effect was minimized.

ACS Style

AnnaChiara D’Urso; Marcello Locatelli; Angela Tartaglia; Linda Molteni; Cristian D’Ovidio; Fabio Savini; James Rudge; Ugo de Grazia. Therapeutic Drug Monitoring of Antiseizure Medications Using Volumetric Absorptive Microsampling: Where Are We? Pharmaceuticals 2021, 14, 627 .

AMA Style

AnnaChiara D’Urso, Marcello Locatelli, Angela Tartaglia, Linda Molteni, Cristian D’Ovidio, Fabio Savini, James Rudge, Ugo de Grazia. Therapeutic Drug Monitoring of Antiseizure Medications Using Volumetric Absorptive Microsampling: Where Are We? Pharmaceuticals. 2021; 14 (7):627.

Chicago/Turabian Style

AnnaChiara D’Urso; Marcello Locatelli; Angela Tartaglia; Linda Molteni; Cristian D’Ovidio; Fabio Savini; James Rudge; Ugo de Grazia. 2021. "Therapeutic Drug Monitoring of Antiseizure Medications Using Volumetric Absorptive Microsampling: Where Are We?" Pharmaceuticals 14, no. 7: 627.

Review
Published: 28 May 2021 in Molecules
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Currently, forensic research is multidisciplinary with new methods and parameters useful to define the cause and time of death as well as survival/agony times. The identification of biochemical markers able to estimate agonal period has been studied by many forensic researchers. It is known that the estimation of agonal time in different types of death is not always easy, hence our interest in literature’s data. The studies analyzed in this review confirm the important role of thanatobiochemistry for the estimation of survival times. Regardless of the death cause, the survival/agony time between the primary event and death influences markers concentrations in biological samples (e.g., blood, urine, cerebrospinal fluid). Different biomarkers can be used for qualitative evaluations in deaths with short and long agony (e.g., C-reactive protein, ferritin, GFAP, etc.). Instead, the quantitative interpretation showed limits due to the lack of reference cut-offs. Thanatobiochemistry is a useful tool to confirm what emerged from autopsies findings (macroscopic and histological analysis), but further studies are desirable to confirm the evidence emerging from our review of the literature.

ACS Style

Enrica Rosato; Martina Bonelli; Marcello Locatelli; Ugo de Grazia; Angela Tartaglia; Fabio Savini; Cristian D'Ovidio. Forensic Biochemical Markers to Evaluate the Agonal Period: A Literature Review. Molecules 2021, 26, 3259 .

AMA Style

Enrica Rosato, Martina Bonelli, Marcello Locatelli, Ugo de Grazia, Angela Tartaglia, Fabio Savini, Cristian D'Ovidio. Forensic Biochemical Markers to Evaluate the Agonal Period: A Literature Review. Molecules. 2021; 26 (11):3259.

Chicago/Turabian Style

Enrica Rosato; Martina Bonelli; Marcello Locatelli; Ugo de Grazia; Angela Tartaglia; Fabio Savini; Cristian D'Ovidio. 2021. "Forensic Biochemical Markers to Evaluate the Agonal Period: A Literature Review." Molecules 26, no. 11: 3259.

Journal article
Published: 26 May 2021 in Journal of Pharmaceutical and Biomedical Analysis
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The reported method aims to be a powerful aid for the simultaneous determination of tetrahydrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG), tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA), and tetrahydrocannabivarin (THCV) in oily based preparations. The chromatographic separation was carried out using an Hypersil Gold PFP (50 × 2.1 mm, 1.9 μm) column, using H2O + 2 mM ammonium formate + 0.2 % formic acid (M1) and Methanol + 2 mM ammonium formate + 0.2 % formic acid (M2) as mobile phases. The flow rate was set 0.4 mL/min. Specifically, this method was validated in terms of linearity, limit of detections and quantifications (LODs and LOQs), accuracy (precision and trueness, both intra and interday), selectivity, and matrix effects. This procedure allowed quantifying seven phytocannabinoids in less than 10 min. The validated method shows a good linearity within the range 0.25−1000 ng/mL, while precision and trueness (intra- and inter-day) were below <13.25 % and 7.59 %, respectively. Regarding the matrix effect, the method satisfies all the requirements, except for the THC and THCV, where it reaches about 120 %. This element does not affect the method performances as it has been observed that this value is constant and reproducible and therefore does not involve errors in the quantitative analysis. The method was tested and applied on more 70 different oily based preparations. Furthermore, starting from four different cannabis cultivar (FM2, Bedrolite, Bedrocan, and Bediol), it allowed to evaluate the reproducibility of the magistrali preparations. The real samples, in fact, derive from different local pharmacies, and were analyzed by the accredited UNI CEI EN ISO/IEC 17025:2018, Pharmatoxicology Laboratory (ACCREDIA, lab n. 2274 ASLPE, accreditation number 1822 L), accordingly to the current regulations.

ACS Style

G.M. Merone; A. Tartaglia; S. Rossi; F. Santavenere; E. Bassotti; C. D’Ovidio; M. Bonelli; E. Rosato; U. de Grazia; A. Zanardo; M. Locatelli; F. Savini. Fast liquid chromatography-tandem mass spectrometry method for the simultaneous determination of phytocannabinoids in oily based preparations. Journal of Pharmaceutical and Biomedical Analysis 2021, 203, 114174 .

AMA Style

G.M. Merone, A. Tartaglia, S. Rossi, F. Santavenere, E. Bassotti, C. D’Ovidio, M. Bonelli, E. Rosato, U. de Grazia, A. Zanardo, M. Locatelli, F. Savini. Fast liquid chromatography-tandem mass spectrometry method for the simultaneous determination of phytocannabinoids in oily based preparations. Journal of Pharmaceutical and Biomedical Analysis. 2021; 203 ():114174.

Chicago/Turabian Style

G.M. Merone; A. Tartaglia; S. Rossi; F. Santavenere; E. Bassotti; C. D’Ovidio; M. Bonelli; E. Rosato; U. de Grazia; A. Zanardo; M. Locatelli; F. Savini. 2021. "Fast liquid chromatography-tandem mass spectrometry method for the simultaneous determination of phytocannabinoids in oily based preparations." Journal of Pharmaceutical and Biomedical Analysis 203, no. : 114174.

Journal article
Published: 02 May 2021 in Journal of Chromatography A
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A new analyte separation and preconcentration method for the trace determination of antidepressant drugs, Fluoxetine (FLU) and Citalopram (CIT) in urine and wastewaters, was developed based on HPLC-DAD analysis after magnetic solid phase extraction (MSPE). In the proposed method, FLU and CIT were retained on the newly synthetized magnetic sorbent ([email protected]) in the presence of buffer (pH 10.0) and then were desorbed into a lower volume of acetonitrile prior to the chromatographic determinations. Before HPLC analysis, all samples were filtered through a 0.45 µm PTFE filter. Experimental parameters such as interaction time, desorption solvent and volume, and pH were studied and optimized in order to establish the detection limit, linearity, enrichment factor and other analytical figures of merit under optimum operation conditions. In the developed method, FLU and CIT were analyzed by diode array detector at the corresponding maximum wavelengths of 227 and 238 nm, respectively, by using an isocratic elution of 60% pH 3.0 buffer, 30% acetonitrile, and 10% methanol. By using the optimum conditions, limit of detections for FLU and CIT were 1.58 and 1.43 ng mL−1, respectively, while the limit of quantifications was 4.82 and 4.71 ng mL−1, respectively. Relative standard deviations (RSD%) for triplicate analyses of model solutions containing 100 ng mL−1 target molecules were found to be less than 5.0 %. Finally, the method was successfully applied to urine (both simulated and real healthy human) and wastewater samples, and quantitative results were obtained in recovery experiments.

ACS Style

Merve Sarıkaya; Halil Ibrahim Ulusoy; Ummugulsum Morgul; Songül Ulusoy; Angela Tartaglia; Erkan Yılmaz; Mustafa Soylak; Marcello Locatelli; Abuzar Kabir. Sensitive determination of Fluoxetine and Citalopram antidepressants in urine and wastewater samples by liquid chromatography coupled with photodiode array detector. Journal of Chromatography A 2021, 1648, 462215 .

AMA Style

Merve Sarıkaya, Halil Ibrahim Ulusoy, Ummugulsum Morgul, Songül Ulusoy, Angela Tartaglia, Erkan Yılmaz, Mustafa Soylak, Marcello Locatelli, Abuzar Kabir. Sensitive determination of Fluoxetine and Citalopram antidepressants in urine and wastewater samples by liquid chromatography coupled with photodiode array detector. Journal of Chromatography A. 2021; 1648 ():462215.

Chicago/Turabian Style

Merve Sarıkaya; Halil Ibrahim Ulusoy; Ummugulsum Morgul; Songül Ulusoy; Angela Tartaglia; Erkan Yılmaz; Mustafa Soylak; Marcello Locatelli; Abuzar Kabir. 2021. "Sensitive determination of Fluoxetine and Citalopram antidepressants in urine and wastewater samples by liquid chromatography coupled with photodiode array detector." Journal of Chromatography A 1648, no. : 462215.

Editorial
Published: 29 April 2021 in Analytica
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In this issue of the Journal “Analytica”, there is a selection of accepted articles, after peer-review process, in which the great importance that Analytical Chemistry plays in the field of Applied Sciences and the vastness of the implications in the various sectors are highlighted

ACS Style

Marcello Locatelli. Analytical Chemistry and Innovative Applications. Analytica 2021, 2, 37 -37.

AMA Style

Marcello Locatelli. Analytical Chemistry and Innovative Applications. Analytica. 2021; 2 (2):37-37.

Chicago/Turabian Style

Marcello Locatelli. 2021. "Analytical Chemistry and Innovative Applications." Analytica 2, no. 2: 37-37.

Journal article
Published: 16 March 2021 in Talanta
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A magnetic solid phase extraction (MSPE) coupled with high-performance liquid chromatography-diode array detection (HPLC-DAD) methodology was developed for the determination of chloramphenicol (CP) and tetracycline (TET) antibiotic residues in milk samples. As a solid phase sorbent, C-nanofiber coated magnetic nanoparticles were synthesized and extensively characterized using Field Emission Scanning Electron Microscopy (FE-SEM), Raman Spectroscopy and X-ray Powder Diffraction (XRD) analysis. Experimental variables of MSPE method for both antibiotic analytes were investigated and optimized systematically. After MSPE, the linear range for both the analytes (r2 > 0.9987) were obtained in a range 10.0–600.0 ng mL−1. The limit of detections (LODs) for CP and TET were 3.02 and 3.52 ng mL-1, respectively while RSDs % were below than 4.0 %. Finally, the developed method based on MPSE-HPLC-DAD was applied to real milk samples to quantify the antibiotic residues. Recovery values for each antibiotic compound were found in the range of 94.6–105.4 % (n = 3) by using spiked model solution.

ACS Style

Busra Vuran; Halil Ibrahim Ulusoy; Gokhan Sarp; Erkan Yilmaz; Ummügülsüm Morgül; Abuzar Kabir; Angela Tartaglia; Marcello Locatelli; Mustafa Soylak. Determination of chloramphenicol and tetracycline residues in milk samples by means of nanofiber coated magnetic particles prior to high-performance liquid chromatography-diode array detection. Talanta 2021, 230, 122307 .

AMA Style

Busra Vuran, Halil Ibrahim Ulusoy, Gokhan Sarp, Erkan Yilmaz, Ummügülsüm Morgül, Abuzar Kabir, Angela Tartaglia, Marcello Locatelli, Mustafa Soylak. Determination of chloramphenicol and tetracycline residues in milk samples by means of nanofiber coated magnetic particles prior to high-performance liquid chromatography-diode array detection. Talanta. 2021; 230 ():122307.

Chicago/Turabian Style

Busra Vuran; Halil Ibrahim Ulusoy; Gokhan Sarp; Erkan Yilmaz; Ummügülsüm Morgül; Abuzar Kabir; Angela Tartaglia; Marcello Locatelli; Mustafa Soylak. 2021. "Determination of chloramphenicol and tetracycline residues in milk samples by means of nanofiber coated magnetic particles prior to high-performance liquid chromatography-diode array detection." Talanta 230, no. : 122307.

Short communication
Published: 08 February 2021 in Natural Product Research
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Anagallis arvensis L. commonly known as ‘Scarlet Pimpernel’ has been used in folklore as natural remedy for treating common ailments. The present research is aimed to explore the phytochemical composition and enzyme inhibition potential of methanol and dichloromethane (DCM) extracts of A. arvensis aerial and root parts. The phytochemical composition was established via HPLC-PDA polyphenolic quantification and UHPLC-MS analysis, while the inhibition potential against amylase and tyrosinase enzymes were assessed using standard in vitro protocols. The HPLC-PDA polyphenolic quantification revealed the presence of important compounds including catechin, gallic acid, chlorogenic acid, and ferulic acid, whereas 34 different secondary metabolites were tentatively identified by UHPLC-MS of both the DCM extracts. All the extracts showed moderate tyrosinase and a weak amylase inhibition activity. The aerial-DCM extract showed comparatively higher tyrosinase and amylase enzyme inhibition potential, which may be due to the presence of secondary metabolites as tentatively identified by its UHPLC-MS profiling. Graphical Abstract

ACS Style

Hammad Saleem; Gokhan Zengin; Marcello Locatelli; Syafiq Asnawi Zainal Abidin; Nafees Ahemad. Investigation of phytochemical composition and enzyme inhibitory potential of Anagallis arvensis L. Natural Product Research 2021, 1 -6.

AMA Style

Hammad Saleem, Gokhan Zengin, Marcello Locatelli, Syafiq Asnawi Zainal Abidin, Nafees Ahemad. Investigation of phytochemical composition and enzyme inhibitory potential of Anagallis arvensis L. Natural Product Research. 2021; ():1-6.

Chicago/Turabian Style

Hammad Saleem; Gokhan Zengin; Marcello Locatelli; Syafiq Asnawi Zainal Abidin; Nafees Ahemad. 2021. "Investigation of phytochemical composition and enzyme inhibitory potential of Anagallis arvensis L." Natural Product Research , no. : 1-6.

Brief report
Published: 20 January 2021 in Analytical Chemistry
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This study introduces an innovative device for the noninvasive sampling and chromatographic analysis of different compounds present in exhaled breath aerosol (EBA). The new sampling device, especially in light of the recent COVID-19 pandemic that forced many countries to impose mandatory facemasks, allows an easy monitoring of the subject’s exposure to different compounds they may come in contact with, actively or passively. The project combines the advantages of a fabric-phase sorptive membrane (FPSM) as an in vivo sampling device with a validated LC-MS/MS screening procedure able to monitor more than 739 chemicals with an overall analysis time of 18 min. The project involves the noninvasive in vivo sampling of the EBA using an FPSM array inserted inside an FFP2 mask. The study involved 15 healthy volunteers, and no restrictions were imposed during or prior to the sampling process regarding the consumption of drinks, food, or drugs. The FPSM array-LC-MS/MS approach allowed us to effectively exploit the advantages of the two complementary procedures (the convenient sampling by an FPSM array and the rapid analysis by LC-MS/MS), obtaining a powerful and green tool to carry out rapid screening analyses for human exposure to different compounds. The flexible fabric substrate, the sponge-like porous architecture of the high-efficiency sol–gel sorbent coating, the availability of a large cache of sorbent coatings, including polar, nonpolar, mixed mode, and zwitterionic phases, the easy installation into the facemask, and the possibility of sampling without interrupting regular activities provide FPSMs unparalleled advantages over other sampling techniques, and their applications are expected to expand to many other clinical or toxicological studies.

ACS Style

Marcello Locatelli; Angela Tartaglia; Halil I. Ulusoy; Songul Ulusoy; Fabio Savini; Sandra Rossi; Francesco Santavenere; Giuseppe M. Merone; Elisa Bassotti; Cristian D’Ovidio; Enrica Rosato; Kenneth G. Furton; Abuzar Kabir. Fabric-Phase Sorptive Membrane Array As a Noninvasive In Vivo Sampling Device For Human Exposure To Different Compounds. Analytical Chemistry 2021, 93, 1957 -1961.

AMA Style

Marcello Locatelli, Angela Tartaglia, Halil I. Ulusoy, Songul Ulusoy, Fabio Savini, Sandra Rossi, Francesco Santavenere, Giuseppe M. Merone, Elisa Bassotti, Cristian D’Ovidio, Enrica Rosato, Kenneth G. Furton, Abuzar Kabir. Fabric-Phase Sorptive Membrane Array As a Noninvasive In Vivo Sampling Device For Human Exposure To Different Compounds. Analytical Chemistry. 2021; 93 (4):1957-1961.

Chicago/Turabian Style

Marcello Locatelli; Angela Tartaglia; Halil I. Ulusoy; Songul Ulusoy; Fabio Savini; Sandra Rossi; Francesco Santavenere; Giuseppe M. Merone; Elisa Bassotti; Cristian D’Ovidio; Enrica Rosato; Kenneth G. Furton; Abuzar Kabir. 2021. "Fabric-Phase Sorptive Membrane Array As a Noninvasive In Vivo Sampling Device For Human Exposure To Different Compounds." Analytical Chemistry 93, no. 4: 1957-1961.

Journal article
Published: 07 December 2020 in Molecules
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The increased risk of illness and disability is related to the age inevitable biological changes. Oxidative stress is a proposed mechanism for many age-related diseases. The crucial importance of polyphenol pharmacophore for aging process is largely described thanks to its effects on concentrations of reactive oxygen species. Resveratrol (3,5,4′-trihydroxy-trans-stilbene, RSV) plays a critical role in slowing the aging process but has a poor bioavailabity after oral intake. In this present work, a series of RSV derivatives was designed, synthesized, and evaluated as potential antioxidant agents. These derivatives contain substituents with different electronic and steric properties in different positions of aromatic rings. This kind of substituents affects the activity and the bioavailability of these compounds compared with RSV used as reference compound. Studies of Log P values demonstrated that the introduction of halogens gives the optimum lipophilicity to be considered promising active agents. Among them, compound 6 showed the higher antioxidant activity than RSV. The presence of trifluoromethyl group together with a chlorine atom increased the antioxidant activity compared to RSV.

ACS Style

Ester Sara Di Filippo; Letizia Giampietro; Barbara De Filippis; Marwa Balaha; Vincenzo Ferrone; Marcello Locatelli; Tiziana Pietrangelo; Angela Tartaglia; Rosa Amoroso; Stefania Fulle. Synthesis and Biological Evaluation of Halogenated E-Stilbenols as Promising Antiaging Agents. Molecules 2020, 25, 5770 .

AMA Style

Ester Sara Di Filippo, Letizia Giampietro, Barbara De Filippis, Marwa Balaha, Vincenzo Ferrone, Marcello Locatelli, Tiziana Pietrangelo, Angela Tartaglia, Rosa Amoroso, Stefania Fulle. Synthesis and Biological Evaluation of Halogenated E-Stilbenols as Promising Antiaging Agents. Molecules. 2020; 25 (23):5770.

Chicago/Turabian Style

Ester Sara Di Filippo; Letizia Giampietro; Barbara De Filippis; Marwa Balaha; Vincenzo Ferrone; Marcello Locatelli; Tiziana Pietrangelo; Angela Tartaglia; Rosa Amoroso; Stefania Fulle. 2020. "Synthesis and Biological Evaluation of Halogenated E-Stilbenols as Promising Antiaging Agents." Molecules 25, no. 23: 5770.

Review
Published: 30 October 2020 in Analytica
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Nowadays, the challenges in analytical chemistry, and mostly in quantitative analysis, include the development and validation of new materials, strategies and procedures to meet the growing need for rapid, sensitive, selective and green methods. In this context, considering the constantly updated International Guidelines, constant innovation is mandatory both in the pre-treatment procedures and in the instrumental configurations to obtain reliable, true, and reproducible information. In this context, additionally to the classic plasma (or serum) matrices, biopsies, whole blood, and urine have seen an increase in the works that also consider non-conventional matrices. Obviously, all these studies have shown that there is a correlation between the blood levels and those found in the new matrix, in order to be able to correlate and compare the results in a robust way and reduce any bias problems. This review provides an update of the most recent developments currently in use in the sample pre-treatment and instrument configurations in the biological/clinical fields. Furthermore, the review concludes with a series of considerations regarding the role and future developments of Analytical Chemistry in light of the forthcoming challenges and new goals to be achieved.

ACS Style

Giuseppe Merone; Angela Tartaglia; Marcello Locatelli; Cristian D’Ovidio; Enrica Rosato; Ugo De Grazia; Francesco Santavenere; Sandra Rossi; Fabio Savini. Analytical Chemistry in the 21st Century: Challenges, Solutions, and Future Perspectives of Complex Matrices Quantitative Analyses in Biological/Clinical Field. Analytica 2020, 1, 44 -59.

AMA Style

Giuseppe Merone, Angela Tartaglia, Marcello Locatelli, Cristian D’Ovidio, Enrica Rosato, Ugo De Grazia, Francesco Santavenere, Sandra Rossi, Fabio Savini. Analytical Chemistry in the 21st Century: Challenges, Solutions, and Future Perspectives of Complex Matrices Quantitative Analyses in Biological/Clinical Field. Analytica. 2020; 1 (1):44-59.

Chicago/Turabian Style

Giuseppe Merone; Angela Tartaglia; Marcello Locatelli; Cristian D’Ovidio; Enrica Rosato; Ugo De Grazia; Francesco Santavenere; Sandra Rossi; Fabio Savini. 2020. "Analytical Chemistry in the 21st Century: Challenges, Solutions, and Future Perspectives of Complex Matrices Quantitative Analyses in Biological/Clinical Field." Analytica 1, no. 1: 44-59.

Liquid chromatography
Published: 15 September 2020 in Analytical Letters
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Polycyclic aromatic hydrocarbons (PAHs) represent a class of organic compounds. Fish oil has been identified as one of the most important contributors to the level of persistent organic pollutants in foods. In fact, fish oils could contain elevated levels of lipophilic pollutants, such as PAHs due to the low metabolization capability of fish. The determination of PAHs in fish oils is complicated due to the fat matrix, which adversely affects both the extraction efficiency and analytical data quality. Target compounds were successfully extracted from nutritional supplements using fabric phase sorptive extraction (FPSE). For elution of the PAHs, the prepared sol-gel phenyl/polydimethylsiloxane (PDMS) coated fabric phase sorptive extraction membranes were back-extracted with acetonitrile and then eluted sample was analyzed by high-performance liquid chromatography (HPLC) with fluorescence detection. The PAHs were eluted on a C18 125 × 4 mm, 5 μm column, the oven temperature was 30 °C, and gradient elution was achieved with acetonitrile and water. The compounds were measured at wavelengths of 270 and 390 nm for pyrene and chrysene and 290 and 430 nm for benzo(a)pyrene. The method was validated in terms of linearity (2.5 to 75 ng/mL for pyrene and chrysene; 2.5 to 100 ng/mL for benzo(a)pyrene), precision and trueness (both intra- and inter-day), and recovery (from 63.2% to 102.3%). The obtained results by applying an innovative FPSE sampling/sample clean-up procedure which has recently shown its advantages in several applications for analysis of biological, environmental, cosmetic, and food matrices are presented in this work for the first time.

ACS Style

Isil Gazioglu; Ozge Sultan Zengin; Angela Tartaglia; Marcello Locatelli; Kenneth G. Furton; Abuzar Kabir. Determination of Polycyclic Aromatic Hydrocarbons in Nutritional Supplements by Fabric Phase Sorptive Extraction (FPSE) with High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection. Analytical Letters 2020, 54, 1683 -1696.

AMA Style

Isil Gazioglu, Ozge Sultan Zengin, Angela Tartaglia, Marcello Locatelli, Kenneth G. Furton, Abuzar Kabir. Determination of Polycyclic Aromatic Hydrocarbons in Nutritional Supplements by Fabric Phase Sorptive Extraction (FPSE) with High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection. Analytical Letters. 2020; 54 (10):1683-1696.

Chicago/Turabian Style

Isil Gazioglu; Ozge Sultan Zengin; Angela Tartaglia; Marcello Locatelli; Kenneth G. Furton; Abuzar Kabir. 2020. "Determination of Polycyclic Aromatic Hydrocarbons in Nutritional Supplements by Fabric Phase Sorptive Extraction (FPSE) with High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection." Analytical Letters 54, no. 10: 1683-1696.

Journal article
Published: 26 August 2020 in Molecules
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Analysis of 4-cyanophenol and 3-nitrophenol was carried out using multi-walled carbon nanotubes-based solid-phase extraction (SPE) and capillary electrophoresis (CE) methods. Capillary electrophoresis was carried out with 18 kV voltage, 214 nm detection, and phosphate buffer (pH 7.0, 15 mM) as background electrolyte at 25 ± 1 °C temperature with 15.05 and 16.5 min migration times of 4-cyanophenol and 3-nitrophenol. The separation and resolution factors were 1.10 and 2.90. The optimized experimental conditions were 40 mg/L concentration, 1.0 g multi-walled carbon nanotubes (MWCNTs) per SPE cartridge, 5.0 mL/min flow rate of water, 0.1 mL flow rate of eluting solvent. The maximum recoveries were 91% and 98% at 0.1 mL/min flow rate of 4-cyanophenol and 3-nitrophenol. These methods were applied successfully for extraction and estimation of 4-cyanophenol and 3-nitrophenol in the municipal wastewater. The reported methods are reproducible, efficient, and practical for the estimation of these phenols in water.

ACS Style

Zeid A. Alothman; Ahmad Yacine Badjah; Marcello Locatelli; Imran Ali. Multi-Walled Carbon Nanotubes Solid-Phase Extraction and Capillary Electrophoresis Methods for the Analysis of 4-Cyanophenol and 3-Nitrophenol in Water. Molecules 2020, 25, 3893 .

AMA Style

Zeid A. Alothman, Ahmad Yacine Badjah, Marcello Locatelli, Imran Ali. Multi-Walled Carbon Nanotubes Solid-Phase Extraction and Capillary Electrophoresis Methods for the Analysis of 4-Cyanophenol and 3-Nitrophenol in Water. Molecules. 2020; 25 (17):3893.

Chicago/Turabian Style

Zeid A. Alothman; Ahmad Yacine Badjah; Marcello Locatelli; Imran Ali. 2020. "Multi-Walled Carbon Nanotubes Solid-Phase Extraction and Capillary Electrophoresis Methods for the Analysis of 4-Cyanophenol and 3-Nitrophenol in Water." Molecules 25, no. 17: 3893.

Journal article
Published: 30 July 2020 in Food Research International
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Calligonum polygonoides L. also known as famine food plant, is normally consumed in times of food scarcity in India and Pakistan and also used traditionally in the management of common diseases. The present design aims to provide an insight into the medicinal potential of four solvent extracts of C. polygonoides via an assessment of its phytochemical profile, antioxidant and enzyme inhibitory potential. Phytochemical composition was estimated by deducing total bioactive constituents, UHPLC-MS secondary metabolites profile, and HPLC phenolic quantification. Antioxidant potential was determined via six methods (radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC), phosphomolybdenum total antioxidant capacity and metal chelation activity). Enzyme inhibitory potential was assessed against clinical enzymes (acetylcholinesterase -AChE, butyrylcholinesterase -BChE, tyrosinase, and α-amylase). The highest amounts of phenolic contents were found in chloroform extract (76.59 mg GAE/g extract) which may be attributed to its higher radical scavenging, reducing power and tyrosinase inhibition potential. The n-butanol extract containing the maximum amount of flavonoids (55.84 mg RE/g extract) exhibited highest metal chelating capacity. Similarly, the n-hexane extract was found to be most active against AChE (4.65 mg GALAE/g extract), BChE (6.59 mg GALAE/g extract), and α-amylase (0.70 mmol ACAE/g extract) enzymes. Secondary metabolite assessment of the crude methanol extract as determined by UHPLC-MS analysis revealed the presence of 24 (negative ionization mode) and 15 (positive ionization mode) secondary metabolites, with most of them belonging to phenolic, flavonoids, terpene, and alkaloid groups. Moreover, gallic acid and naringenin were the main phenolics quantified by HPLC-PDA analysis in all the tested extracts (except n-butanol extract). PCA statistical analysis was also conducted to establish any possible relationship amongst bioactive contents and biological activities. Overall, the C. polygonoides extracts could be further considered to isolate bioactive enzyme inhibitory and antioxidant natural phytocompounds.

ACS Style

Irfan Pervaiz; Hammad Saleem; Muhammad Sarfraz; Muhammad Imran Tousif; Umair Khurshid; Saeed Ahmad; Gokhan Zengin; Kouadio Ibrahime Sinan; Marcello Locatelli; Fawzi M. Mahomoodally; Syafiq Asnawi Zainal Abidin; Nafees Ahemad. Multidirectional insights into the phytochemical, biological, and multivariate analysis of the famine food plant (Calligonum polygonoides L).: A novel source of bioactive phytocompounds. Food Research International 2020, 137, 109606 .

AMA Style

Irfan Pervaiz, Hammad Saleem, Muhammad Sarfraz, Muhammad Imran Tousif, Umair Khurshid, Saeed Ahmad, Gokhan Zengin, Kouadio Ibrahime Sinan, Marcello Locatelli, Fawzi M. Mahomoodally, Syafiq Asnawi Zainal Abidin, Nafees Ahemad. Multidirectional insights into the phytochemical, biological, and multivariate analysis of the famine food plant (Calligonum polygonoides L).: A novel source of bioactive phytocompounds. Food Research International. 2020; 137 ():109606.

Chicago/Turabian Style

Irfan Pervaiz; Hammad Saleem; Muhammad Sarfraz; Muhammad Imran Tousif; Umair Khurshid; Saeed Ahmad; Gokhan Zengin; Kouadio Ibrahime Sinan; Marcello Locatelli; Fawzi M. Mahomoodally; Syafiq Asnawi Zainal Abidin; Nafees Ahemad. 2020. "Multidirectional insights into the phytochemical, biological, and multivariate analysis of the famine food plant (Calligonum polygonoides L).: A novel source of bioactive phytocompounds." Food Research International 137, no. : 109606.

Editorial
Published: 21 July 2020 in Analytica
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Back in 1894, Wilhelm Ostwald defined analytical chemistry as “the art of recognizing different substances and determining their constituents”, which “occupies a prominent position among the applications of science, since the questions it allows us to answer arise wherever chemical processes are used for scientific or technical purposes”

ACS Style

Marcello Locatelli; Roberto Mandrioli; Victoria Samanidou; Thomas W. Bocklitz. Analytica—A Journal of Analytical Chemistry and Chemical Analysis. Analytica 2020, 1, 12 -13.

AMA Style

Marcello Locatelli, Roberto Mandrioli, Victoria Samanidou, Thomas W. Bocklitz. Analytica—A Journal of Analytical Chemistry and Chemical Analysis. Analytica. 2020; 1 (1):12-13.

Chicago/Turabian Style

Marcello Locatelli; Roberto Mandrioli; Victoria Samanidou; Thomas W. Bocklitz. 2020. "Analytica—A Journal of Analytical Chemistry and Chemical Analysis." Analytica 1, no. 1: 12-13.

Journal article
Published: 15 July 2020 in Reviews in Separation Sciences
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[icon name="unlock"] OPEN-ACCESS [icon name="eye"] PEER-REVIEWED REVIEW Angela Tartaglia, Francesca D’Ambrosio, Piera Ramundo, Vincenzo Ferrone, Daniele Ricci,Marcello Locatelli* University “G.... The QuEChERS method, acronym of quick, easy, cheap, effective, rugged and safe is one of the pre-treatment techniques that has gained great popularity.

ACS Style

Angela Tartaglia; Francesca D’Ambrosio; Piera Ramundo; Vincenzo Ferrone; Daniele Ricci; Marcello Locatelli. Innovative approach to increase sensibility and selectivity in analytical chemistry: QuEChERS method. Reviews in Separation Sciences 2020, 2, 19 -34.

AMA Style

Angela Tartaglia, Francesca D’Ambrosio, Piera Ramundo, Vincenzo Ferrone, Daniele Ricci, Marcello Locatelli. Innovative approach to increase sensibility and selectivity in analytical chemistry: QuEChERS method. Reviews in Separation Sciences. 2020; 2 (1):19-34.

Chicago/Turabian Style

Angela Tartaglia; Francesca D’Ambrosio; Piera Ramundo; Vincenzo Ferrone; Daniele Ricci; Marcello Locatelli. 2020. "Innovative approach to increase sensibility and selectivity in analytical chemistry: QuEChERS method." Reviews in Separation Sciences 2, no. 1: 19-34.

Communication
Published: 29 June 2020 in Processes
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The current research work is an endeavor to study the chemical profiling and enzyme-inhibition potential of different polarity solvent (n-hexane, dichloromethane—DCM and methanol—MeOH) extracts from the aerial and stem parts of Buxus papillosa C.K. Schneid. All the extracts were analyzed for HPLC-PDA phenolic quantification, while both (aerial and stem) DCM extracts were studied for UHPLC-MS phytochemical composition. The inhibitory activity against the clinically important enzymes having crucial role in different pathologies like skin diseases (tyrosinase), inflammatory problems (lipoxygenase—LOX) and diabetes mellitus (α-amylase) were studied using standard in vitro bioassays. The DCM extracts upon UHPLC-MS analysis conducted in both negative and positive ionization modes has led to the tentative identification of 52 important secondary metabolites. Most of these belonged to the alkaloid, flavonoid, phenolic and triterpenoid classes. The HPLC-PDA polyphenolic quantification identified the presence of 10 phenolic compounds. Catechin was present in significant amounts in aerial-MeOH (7.62 ± 0.45 μg/g extract) and aerial-DCM (2.39 ± 0.51-μg/g extract) extracts. Similarly, higher amounts of epicatechin (2.76 ± 0.32-μg/g extract) and p-hydroxybenzoic acid (1.06 ± 0.21 μg/g extract) were quantified in aerial-DCM and stem-MeOH extracts, respectively. Likewise, all the extracts exhibited moderate inhibition against all the tested enzymes. These findings explain the wide usage of this plant in folklore medicine and suggest that it could be further studied as an origin of novel bioactive phytocompounds and for the designing of new pharmaceuticals.

ACS Style

Hammad Saleem; Thet Thet Htar; Rakesh Naidu; Gokhan Zengin; Marcello Locatelli; Angela Tartaglia; Syafiq Asnawi Zainal Abidin; Nafees Ahemad. Phytochemical Composition and Enzyme Inhibition Studies of Buxus papillosa C.K. Schneid. Processes 2020, 8, 757 .

AMA Style

Hammad Saleem, Thet Thet Htar, Rakesh Naidu, Gokhan Zengin, Marcello Locatelli, Angela Tartaglia, Syafiq Asnawi Zainal Abidin, Nafees Ahemad. Phytochemical Composition and Enzyme Inhibition Studies of Buxus papillosa C.K. Schneid. Processes. 2020; 8 (7):757.

Chicago/Turabian Style

Hammad Saleem; Thet Thet Htar; Rakesh Naidu; Gokhan Zengin; Marcello Locatelli; Angela Tartaglia; Syafiq Asnawi Zainal Abidin; Nafees Ahemad. 2020. "Phytochemical Composition and Enzyme Inhibition Studies of Buxus papillosa C.K. Schneid." Processes 8, no. 7: 757.

Journal article
Published: 17 June 2020 in Pharmaceutics
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Mesoporous silicon microparticles (MSMPs) can incorporate drug-carrying nanoparticles (NPs) into their pores. An NP-loaded MSMP is a multistage vector (MSV) that forms a Matryoshka-like structure that protects the therapeutic cargo from degradation and prevents its dilution in the circulation during delivery to tumor cells. We developed an MSV constituted by 1 µm discoidal MSMPs embedded with PEGylated liposomes containing oxaliplatin (oxa) which is a therapeutic agent for colorectal cancer (CRC). To obtain extra-small liposomes able to fit the 60 nm pores of MSMP, we tested several liposomal formulations, and identified two optimal compositions, with a prevalence of the rigid lipid 1,2-distearoyl-sn-glycero-3-phosphocholine and of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]. To improve the MSV assembly, we optimized the liposome-loading inside the MSMP and achieved a five-fold increase of the payload using an innovative lyophilization approach. This procedure also increased the load and limited dimensional changes of the liposomes released from the MSV in vitro. Lastly, we found that the cytotoxic efficacy of oxa-loaded liposomes and-oxa-liposome-MSV in CRC cell culture was similar to that of free oxa. This study increases knowledge about extra-small liposomes and their loading into porous materials and provides useful hints about alternative strategies for designing drug-encapsulating NPs.

ACS Style

Armando Cevenini; Christian Celia; Stefania Orrù; Daniela Sarnataro; Maddalena Raia; Valentina Mollo; Marcello Locatelli; Esther Imperlini; Nicoletta Peluso; Rosa Peltrini; Enrica De De Rosa; Alessandro Parodi; Luigi Del Del Vecchio; Luisa Di Di Marzio; Massimo Fresta; Paolo Antonio Netti; Haifa Shen; Xuewu Liu; Ennio Tasciotti; Francesco Salvatore. Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy. Pharmaceutics 2020, 12, 559 .

AMA Style

Armando Cevenini, Christian Celia, Stefania Orrù, Daniela Sarnataro, Maddalena Raia, Valentina Mollo, Marcello Locatelli, Esther Imperlini, Nicoletta Peluso, Rosa Peltrini, Enrica De De Rosa, Alessandro Parodi, Luigi Del Del Vecchio, Luisa Di Di Marzio, Massimo Fresta, Paolo Antonio Netti, Haifa Shen, Xuewu Liu, Ennio Tasciotti, Francesco Salvatore. Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy. Pharmaceutics. 2020; 12 (6):559.

Chicago/Turabian Style

Armando Cevenini; Christian Celia; Stefania Orrù; Daniela Sarnataro; Maddalena Raia; Valentina Mollo; Marcello Locatelli; Esther Imperlini; Nicoletta Peluso; Rosa Peltrini; Enrica De De Rosa; Alessandro Parodi; Luigi Del Del Vecchio; Luisa Di Di Marzio; Massimo Fresta; Paolo Antonio Netti; Haifa Shen; Xuewu Liu; Ennio Tasciotti; Francesco Salvatore. 2020. "Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy." Pharmaceutics 12, no. 6: 559.

Journal article
Published: 15 June 2020 in Journal of Applied Bioanalysis
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Marcello Locatelli. Perspectives on the Multidisciplinary role of Bioanalysis: an Interview with Prof. Marcello Locatelli. Journal of Applied Bioanalysis 2020, 6, 71 -75.

AMA Style

Marcello Locatelli. Perspectives on the Multidisciplinary role of Bioanalysis: an Interview with Prof. Marcello Locatelli. Journal of Applied Bioanalysis. 2020; 6 (2):71-75.

Chicago/Turabian Style

Marcello Locatelli. 2020. "Perspectives on the Multidisciplinary role of Bioanalysis: an Interview with Prof. Marcello Locatelli." Journal of Applied Bioanalysis 6, no. 2: 71-75.