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Dr. Gokhan Zengin
Department of Biology, Science Faculty, Selçuk Üniversitesi, 42130 Konya, Turkey

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0 phenolic compounds
0 antioxidant capacity
0 Purification and characterization of biomolecules
0 Enzyme inhibitory mechanisms and assays
0 Nutraceutical and functional foods

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https://www.researchgate.net/profile/Gokhan-Zengin-2

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Journal article
Published: 30 August 2021 in Antioxidants
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Epilobium hirsutum is extensively used as a traditional remedy in folk medicine, especially against prostate inflammation. Therefore, we evaluated the chemical profiles and biopharmaceutical potentials of different extracts of E. hirsutum aerial parts and roots. Metabolomic, antioxidant, and enzyme inhibitory profiles were investigated. Human prostate cancer PC3 cells were exposed to the extracts to evaluate antiproliferative effects. Gene expression and bioinformatics analyses were performed to investigate anti-inflammatory mechanisms. Oenothein B and myricetin were prominent compounds in the extracts. In scavenging/reducing assays, the methanol, infusion, and methanol/water extracts exhibited similar activities. We also observed the reduction of PC3 viability occurring following exposure to methanol and methanol/water extracts. According to bioinformatics analysis, myricetin was predicted to interact with COX-2 and TNFα. The interaction between TNFα and oxo-dihydroxy-octadecenoic acid was predicted as well. Intriguingly, the gene expression of COX-2 and TNFα was reduced in PC3 cells after exposure to methanol and methanol/water extracts. These effects were paralleled by the decreased gene expression of IL-8 and NFkB and the inhibition of PGE2 release. Therefore, the present findings suggest the potential use of E. hirsutum for the management of the burden of inflammation and oxidative stress occurring in lower urinary tract diseases, including prostatitis.

ACS Style

Gunes Ak; Gokhan Zengin; Mohamad Fawzi Mahomoodally; Eulogio Llorent-Martínez; Giustino Orlando; Annalisa Chiavaroli; Luigi Brunetti; Lucia Recinella; Sheila Leone; Simonetta Cristina Di Simone; Luigi Menghini; Claudio Ferrante. Shedding Light into the Connection between Chemical Components and Biological Effects of Extracts from Epilobium hirsutum: Is It a Potent Source of Bioactive Agents from Natural Treasure? Antioxidants 2021, 10, 1389 .

AMA Style

Gunes Ak, Gokhan Zengin, Mohamad Fawzi Mahomoodally, Eulogio Llorent-Martínez, Giustino Orlando, Annalisa Chiavaroli, Luigi Brunetti, Lucia Recinella, Sheila Leone, Simonetta Cristina Di Simone, Luigi Menghini, Claudio Ferrante. Shedding Light into the Connection between Chemical Components and Biological Effects of Extracts from Epilobium hirsutum: Is It a Potent Source of Bioactive Agents from Natural Treasure? Antioxidants. 2021; 10 (9):1389.

Chicago/Turabian Style

Gunes Ak; Gokhan Zengin; Mohamad Fawzi Mahomoodally; Eulogio Llorent-Martínez; Giustino Orlando; Annalisa Chiavaroli; Luigi Brunetti; Lucia Recinella; Sheila Leone; Simonetta Cristina Di Simone; Luigi Menghini; Claudio Ferrante. 2021. "Shedding Light into the Connection between Chemical Components and Biological Effects of Extracts from Epilobium hirsutum: Is It a Potent Source of Bioactive Agents from Natural Treasure?" Antioxidants 10, no. 9: 1389.

Full paper
Published: 27 August 2021 in Chemistry & Biodiversity
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Among the alternatives to environmentally toxic and socio-economically unacceptable chemical pesticides, essential oils from Ocimum gratissimum and Cymbopogon citratus were tested on the main pests and beneficial insects of the cotton plant in Côte d'Ivoire. After extraction and chemical analysis of the essential oils, field trials were carried out using a Fisher block system with three treatment repetitions where their effects compared with those of a registered synthetic insecticide (IBIS A 52 EC). Foliar applications of the products were carried out in accordance with the cotton plant protection extension programme in Côte d'Ivoire from the 45th to the 115th day after plant emergence, with one application every fortnight. Twenty-three and forty compounds representing about 96 and 99 % of the oil composition of O. gratissimum and C. citratus respectively were elucidated. The most abundant compounds were p-cymene and thymol (O. gratissimum) and myrcene, neral and geranial (C. citratus). The essential oil of O. gratissimum at concentrations of 2 and 5 % showed insecticidal activity on all pests (biting-sucking and carpophagous), except the phyllophagous Syllepte derogata. C. citratus, at a low concentration (1 %), was particularly toxic to whiteflies (Bemisia tabaci), however it favoured the action of beneficial insects, specifically black ants and ladybirds in the cotton plots, unlike the chemical product. EO of O. gratissimum (1.60 and 4.62 mg GALAE/g, respectively) and C. citratus (2.26 and 2.78 mg GALAE, respectively) exhibited also significant acetyl and butyryl cholinesterase inhibitors. Insecticide formulations based on the essential oils of O. gratissimum and C. citratus offer favourable prospects for their use in cotton cultivation as an alternative to chemical pesticides.

ACS Style

Koffi Christophe Kobenan; Kouadio Kra Norbert Bini; Malanno Kouakou; Kouadio Ibrahime Sinan; Gokhan Zengin; Germain Elisabeth Cynthia Ochou; Nina Reine Kosso Boka; Philippe Menozzi; Ochou Germain Ochou; Acka Emmanuel Dick. Chemical composition and spectrum of insecticidal activity of the essential oils of Ocimumgratissimum L. and Cymbopogoncitratus Stapf on the main insects of the cotton entomofauna in Côte d’Ivoire. Chemistry & Biodiversity 2021, 1 .

AMA Style

Koffi Christophe Kobenan, Kouadio Kra Norbert Bini, Malanno Kouakou, Kouadio Ibrahime Sinan, Gokhan Zengin, Germain Elisabeth Cynthia Ochou, Nina Reine Kosso Boka, Philippe Menozzi, Ochou Germain Ochou, Acka Emmanuel Dick. Chemical composition and spectrum of insecticidal activity of the essential oils of Ocimumgratissimum L. and Cymbopogoncitratus Stapf on the main insects of the cotton entomofauna in Côte d’Ivoire. Chemistry & Biodiversity. 2021; ():1.

Chicago/Turabian Style

Koffi Christophe Kobenan; Kouadio Kra Norbert Bini; Malanno Kouakou; Kouadio Ibrahime Sinan; Gokhan Zengin; Germain Elisabeth Cynthia Ochou; Nina Reine Kosso Boka; Philippe Menozzi; Ochou Germain Ochou; Acka Emmanuel Dick. 2021. "Chemical composition and spectrum of insecticidal activity of the essential oils of Ocimumgratissimum L. and Cymbopogoncitratus Stapf on the main insects of the cotton entomofauna in Côte d’Ivoire." Chemistry & Biodiversity , no. : 1.

Journal article
Published: 20 August 2021 in Food Research International
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In this paper, an A-type procyanidin (PAC)-rich cranberry extract (CB-B) was obtained mixing different extracts and was formulated with D-mannose and ascorbic acid to obtain a novel nutraceutical (URO-F) aimed at preventing non-complicated bacterial urinary tract infections (UTIs). To assess the bioactivity of CB-B and URO-F, urine samples collected from six healthy volunteers undergoing a 2-days oral consumption of 0.41 g/day of CB-B or 10 g/day of URO-F (corresponding to 72 mg/day of PACs) were tested against uropathogenic E. coli (UPEC) incubated on urinary bladder epithelial cells (T24). Urinary markers of CB-B and URO-F consumption were assessed in the same urine output by UPLC-QTOF-based untargeted metabolomics approach. CB-B and URO-F were evaluated for their ability to promote the intestinal barrier function by restoring the trans-epithelial electrical resistance (TEER) and to inhibit the production of inflammatory cytokines in intestinal epithelial Caco2 cells. CB-B was characterized by a high PAC-A content (70% of total PACs) and a broad distribution of different PACs polymers (dimers-hexamers). Urine from subjects consuming CB-B and URO-F showed a significant effect in reducing the adhesion of UPEC to urothelium in vitro, supporting their efficacy as anti-adhesive agents after oral intake. CB-B inhibited the release of cytokine IL-8, and both products were effective in restoring the TEER. Overall, our results show that the beneficial effects of CB-B and URO-F on UTIs are not only due to the antiadhesive activity of cranberry on UPEC in the urothelium, but also to a multi-target activity involving anti-inflammatory and permeability-enhancing effects on intestinal epithelium.

ACS Style

Marta Faggian; Giulia Bernabè; Marco Valente; Stefano Francescato; Gianni Baratto; Paola Brun; Ignazio Castagliuolo; Stefano Dall'Acqua; Gregorio Peron. Characterization of PACs profile and bioactivity of a novel nutraceutical combining cranberry extracts with different PAC-A oligomers, D-mannose and ascorbic acid: An in vivo/ex vivo evaluation of dual mechanism of action on intestinal barrier and urinary epithelium. Food Research International 2021, 149, 110649 .

AMA Style

Marta Faggian, Giulia Bernabè, Marco Valente, Stefano Francescato, Gianni Baratto, Paola Brun, Ignazio Castagliuolo, Stefano Dall'Acqua, Gregorio Peron. Characterization of PACs profile and bioactivity of a novel nutraceutical combining cranberry extracts with different PAC-A oligomers, D-mannose and ascorbic acid: An in vivo/ex vivo evaluation of dual mechanism of action on intestinal barrier and urinary epithelium. Food Research International. 2021; 149 ():110649.

Chicago/Turabian Style

Marta Faggian; Giulia Bernabè; Marco Valente; Stefano Francescato; Gianni Baratto; Paola Brun; Ignazio Castagliuolo; Stefano Dall'Acqua; Gregorio Peron. 2021. "Characterization of PACs profile and bioactivity of a novel nutraceutical combining cranberry extracts with different PAC-A oligomers, D-mannose and ascorbic acid: An in vivo/ex vivo evaluation of dual mechanism of action on intestinal barrier and urinary epithelium." Food Research International 149, no. : 110649.

Full paper
Published: 16 August 2021 in Chemistry & Biodiversity
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The aim of the present study was to quantify selected phenolic compounds, determine antioxidant activity and enzyme inhibitory effects of the aerial parts of Alkanna trichophylla Hub.-Mor. ( A. trichophylla ) and Convolvulus galaticus Rost.ex Choisy ( C. galaticus ) extracts prepared by homogenizer-assisted extraction (HAE), maceration (MAC) and infusion techniques. This is the first time such report has been designed to validate the phytochemical composition and bioactivity of these plants. Multivariate analysis was conducted on collected data. Rutin and caffeoylquinic acid derivatives were the most significant compounds in A. trichophylla and C. galaticus , respectively. The highest antioxidant activity of A. trichophylla was mostly exhibited by HAE/methanolic extracts as determined by DPPH, ABTS, FRAP (51.39, 112.70 and 145.73 mg TE/g, respectively) and phosphomolybdenum (2.05 mmol TE/g) assays. However, significant antioxidant activities varied within the extracts of C. galaticus . HAE/methanolic extract of A. trichophylla significantly depressed AChE (2.70 mg GALAE/g), BChE (5.53 mg GALAE/g) and tyrosinase (26.34 mg KAE/g) activities and that of C. galaticus inhibited AChE (2.04 mg GALAE/g), tyrosinase (31.25 mg KAE/g) and α-amylase (0.53 mmol ACAE/g) activities significantly. We conclude that HAE was the most efficient extraction technique as high yield of compounds and promising bioactivities were recorded from extracts prepared. Multivariate analysis showed that types of solvents influence recovery of compounds and biological activities. This research study can be used as one methodological starting point for further investigation on these plants as all results are clearly promising and open the door to further research challenges such as cytotoxicity evaluation, molecular docking analysis, and more screening of pharmacological actions.

ACS Style

Mohamad Fawzi Mahomoodally; Gokhan Zengin; Kouadio Ibrahime Sinan; Gunes Ak; Nabeelah Bibi Sadeer; Simone Angeloni; Ahmed M. Mustafa; Giovanni Caprioli; Filippo Maggi; Ugur Cakilcioglu; Alevcan Kaplan; Ebru Yuce Babacan; A. Bouyahya; Ekrem Darendelioglu. Two medicinal plants (Alkanna trichophila and Convolvulus galaticus) from Turkey: Chemical characterization and biological perspectives. Chemistry & Biodiversity 2021, 1 .

AMA Style

Mohamad Fawzi Mahomoodally, Gokhan Zengin, Kouadio Ibrahime Sinan, Gunes Ak, Nabeelah Bibi Sadeer, Simone Angeloni, Ahmed M. Mustafa, Giovanni Caprioli, Filippo Maggi, Ugur Cakilcioglu, Alevcan Kaplan, Ebru Yuce Babacan, A. Bouyahya, Ekrem Darendelioglu. Two medicinal plants (Alkanna trichophila and Convolvulus galaticus) from Turkey: Chemical characterization and biological perspectives. Chemistry & Biodiversity. 2021; ():1.

Chicago/Turabian Style

Mohamad Fawzi Mahomoodally; Gokhan Zengin; Kouadio Ibrahime Sinan; Gunes Ak; Nabeelah Bibi Sadeer; Simone Angeloni; Ahmed M. Mustafa; Giovanni Caprioli; Filippo Maggi; Ugur Cakilcioglu; Alevcan Kaplan; Ebru Yuce Babacan; A. Bouyahya; Ekrem Darendelioglu. 2021. "Two medicinal plants (Alkanna trichophila and Convolvulus galaticus) from Turkey: Chemical characterization and biological perspectives." Chemistry & Biodiversity , no. : 1.

Journal article
Published: 06 August 2021 in International Journal of Molecular Sciences
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Endometriosis, an estrogen-dependent chronic gynecological disease, is characterized by a systemic inflammation that affects circulating red blood cells (RBC), by reducing anti-oxidant defenses. The aim of this study was to investigate the potential beneficial effects of licorice intake to protect RBCs from dapsone hydroxylamine (DDS-NHOH), a harmful metabolite of dapsone, commonly used in the treatment of many diseases. A control group (CG, n = 12) and a patient group (PG, n = 18) were treated with licorice extract (25 mg/day), for a week. Blood samples before (T0) and after (T1) treatment were analyzed for: i) band 3 tyrosine phosphorylation and high molecular weight aggregates; and ii) glutathionylation and carbonic anhydrase activity, in the presence or absence of adjunctive oxidative stress induced by DDS-NHOH. Results were correlated with plasma glycyrrhetinic acid (GA) concentrations, measured by HPLC–MS. Results showed that licorice intake decreased the level of DDS-NHOH-related oxidative alterations in RBCs, and the reduction was directly correlated with plasma GA concentration. In conclusion, in PG, the inability to counteract oxidative stress is a serious concern in the evaluation of therapeutic approaches. GA, by protecting RBC from oxidative assault, as in dapsone therapy, might be considered as a new potential tool for preventing further switching into severe endometriosis.

ACS Style

Chiara Sabbadin; Alessandra Andrisani; Gabriella Donà; Elena Tibaldi; Anna Brunati; Stefano Dall’Acqua; Eugenio Ragazzi; Guido Ambrosini; Decio Armanini; Luciana Bordin. Endometriosis Susceptibility to Dapsone-Hydroxylamine-Induced Alterations Can Be Prevented by Licorice Intake: In Vivo and In Vitro Study. International Journal of Molecular Sciences 2021, 22, 8476 .

AMA Style

Chiara Sabbadin, Alessandra Andrisani, Gabriella Donà, Elena Tibaldi, Anna Brunati, Stefano Dall’Acqua, Eugenio Ragazzi, Guido Ambrosini, Decio Armanini, Luciana Bordin. Endometriosis Susceptibility to Dapsone-Hydroxylamine-Induced Alterations Can Be Prevented by Licorice Intake: In Vivo and In Vitro Study. International Journal of Molecular Sciences. 2021; 22 (16):8476.

Chicago/Turabian Style

Chiara Sabbadin; Alessandra Andrisani; Gabriella Donà; Elena Tibaldi; Anna Brunati; Stefano Dall’Acqua; Eugenio Ragazzi; Guido Ambrosini; Decio Armanini; Luciana Bordin. 2021. "Endometriosis Susceptibility to Dapsone-Hydroxylamine-Induced Alterations Can Be Prevented by Licorice Intake: In Vivo and In Vitro Study." International Journal of Molecular Sciences 22, no. 16: 8476.

Journal article
Published: 06 August 2021 in Food Chemistry
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In this study, the aerial parts and bulbs of nine Allium species were investigated for their functional phytochemical profile, in vitro antioxidant activities, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase, α-glucosidase, and tyrosinase inhibitory properties. Phenolics, alkaloids, glucosinolates and other sulfur-containing compounds were distinctively profiled in the different species. Maceration in methanol allowed recovering the highest cumulative phenolic content in A. scabrifolium (42.31 mg/g), followed by A. goekyigiti (33.15 mg/g) and A. atroviolaceum (28.35 mg/g). The aerial parts of all Allium species showed high in vitro antioxidant activity whereas methanolic extract of A. cappadocicum bulb showed the highest inhibition against AChE (2.44 mg galantamine equivalent/g) and the water extracts of A. isauricum aerial part were the best BChE inhibitors (4.31 mg galantamine equivalent/g). Bulbs were the richer source of oligosaccharides, and in vitro digestion determined an increase of oligosaccharides bioaccessibility. A promising nutraceutical potential could be highlighted in our understudied Allium species.

ACS Style

Gabriele Rocchetti; Leilei Zhang; Serena Bocchi; Gianluca Giuberti; Gunes Ak; Fevzi Elbasan; Evren Yıldıztugay; Ramazan Ceylan; Marie Carene Nancy Picot-Allain; Mohamad Fawzi Mahomoodally; Luigi Lucini; Gokhan Zengin. The functional potential of nine Allium species related to their untargeted phytochemical characterization, antioxidant capacity and enzyme inhibitory ability. Food Chemistry 2021, 368, 130782 .

AMA Style

Gabriele Rocchetti, Leilei Zhang, Serena Bocchi, Gianluca Giuberti, Gunes Ak, Fevzi Elbasan, Evren Yıldıztugay, Ramazan Ceylan, Marie Carene Nancy Picot-Allain, Mohamad Fawzi Mahomoodally, Luigi Lucini, Gokhan Zengin. The functional potential of nine Allium species related to their untargeted phytochemical characterization, antioxidant capacity and enzyme inhibitory ability. Food Chemistry. 2021; 368 ():130782.

Chicago/Turabian Style

Gabriele Rocchetti; Leilei Zhang; Serena Bocchi; Gianluca Giuberti; Gunes Ak; Fevzi Elbasan; Evren Yıldıztugay; Ramazan Ceylan; Marie Carene Nancy Picot-Allain; Mohamad Fawzi Mahomoodally; Luigi Lucini; Gokhan Zengin. 2021. "The functional potential of nine Allium species related to their untargeted phytochemical characterization, antioxidant capacity and enzyme inhibitory ability." Food Chemistry 368, no. : 130782.

Review article
Published: 03 August 2021 in Plant Physiology and Biochemistry
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Plants and microbes interact with each other via different chemical signaling pathways. At the risophere level, the microbes can secrete molecules, called elicitors, which act on their receptors located in plant cells. The so-called elicitor molecules as well as their actions differ according to the mcirobes and induce different bilogical responses in plants such as the synthesis of secondary metabolites. Microbial compounds induced phenotype changes in plants are known as elicitors and signaling pathways which integrate elicitor's signals in plants are called elicitation. In this review, the impact of microbial elicitors on the synthesis and the secretion of secondary metabolites in plants was highlighted. Moreover, biological properties of these bioactive compounds were also highlighted and discussed. Indeed, several bacteria, fungi, and viruses release elicitors which bind to plant cell receptors and mediate signaling pathways involved in secondary metabolites synthesis. Different phytochemical classes such as terpenoids, phenolic acids and flavonoids were synthesized and/or increased in medicinal plants via the action of microbial elicitors. Moreover, these compounds compounds exhibit numerous biological activities and can therefore be explored in drugs discovery.

ACS Style

Imane Chamkhi; Taoufiq Benali; Tarik Aanniz; Naoual El Menyiy; Fatima-Ezzahrae Guaouguaou; Nasreddine El Omari; Mohamed El-Shazly; Gokhan Zengin; Abdelhakim Bouyahya. Plant-microbial interaction: The mechanism and the application of microbial elicitor induced secondary metabolites biosynthesis in medicinal plants. Plant Physiology and Biochemistry 2021, 167, 269 -295.

AMA Style

Imane Chamkhi, Taoufiq Benali, Tarik Aanniz, Naoual El Menyiy, Fatima-Ezzahrae Guaouguaou, Nasreddine El Omari, Mohamed El-Shazly, Gokhan Zengin, Abdelhakim Bouyahya. Plant-microbial interaction: The mechanism and the application of microbial elicitor induced secondary metabolites biosynthesis in medicinal plants. Plant Physiology and Biochemistry. 2021; 167 ():269-295.

Chicago/Turabian Style

Imane Chamkhi; Taoufiq Benali; Tarik Aanniz; Naoual El Menyiy; Fatima-Ezzahrae Guaouguaou; Nasreddine El Omari; Mohamed El-Shazly; Gokhan Zengin; Abdelhakim Bouyahya. 2021. "Plant-microbial interaction: The mechanism and the application of microbial elicitor induced secondary metabolites biosynthesis in medicinal plants." Plant Physiology and Biochemistry 167, no. : 269-295.

Original article
Published: 28 July 2021 in Journal of Food Processing and Preservation
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Propolis, a resinous substance collected by honeybees from plants, is well-known worldwide by its diversified biological activities. This study was aimed to determine the chemical composition of four ethanol extract propolis (EEP) collected from Morocco and to investigate thier antibacterial and antioxidant activities. The chemical composition was determined by Ultra-High Performance Liquid Chromatography-tandem mass spectrometry (UHPLC/MS). The antibacterial activity was evaluated using the well diffusion and microdilution methods. The antioxidant activity was investigated in vitro using three complementary techniques, namely reduction power, β-carotene bleaching, and DPPH scavenging assays. The identified molecules belong mainly to the flavonoid family, and interestingly, several of them were flavonoid glycosides. The four extracts showed strong antibacterial activity. The inhibitory zones varied from 10.00±00 to 22.5±2.12 mm, and MIC values ranged from 0.15 to > 5mg/ml, while MBC values ranged from 1.25 to > 5mg/ml. The antioxidant activity was relatively strong compared to positive controls, with IC50 varied from 67.61 to 140.04 µg/mL against DPPH free radical, and a relative antioxidant activity varied from 68.40±3.90 to 100.92±4.24, determined by β-carotene bleaching test. The antioxidant property was related to the chemical constituents. These results indicate that Moroccan propolis can be a valuable source of bioactive compounds for further drug discovery.

ACS Style

Omar Belmehdi; Abdelhakim Bouyahya; József Jekő; Zoltán Cziáky; Gokhan Zengin; Gyula Sotkó; Aicha El Baaboua; Nadia Skali Senhaji; Jamal Abrini. Chemical analysis, antibacterial, and antioxidant activities of flavonoid‐rich extracts from four Moroccan propolis. Journal of Food Processing and Preservation 2021, e15816 .

AMA Style

Omar Belmehdi, Abdelhakim Bouyahya, József Jekő, Zoltán Cziáky, Gokhan Zengin, Gyula Sotkó, Aicha El Baaboua, Nadia Skali Senhaji, Jamal Abrini. Chemical analysis, antibacterial, and antioxidant activities of flavonoid‐rich extracts from four Moroccan propolis. Journal of Food Processing and Preservation. 2021; ():e15816.

Chicago/Turabian Style

Omar Belmehdi; Abdelhakim Bouyahya; József Jekő; Zoltán Cziáky; Gokhan Zengin; Gyula Sotkó; Aicha El Baaboua; Nadia Skali Senhaji; Jamal Abrini. 2021. "Chemical analysis, antibacterial, and antioxidant activities of flavonoid‐rich extracts from four Moroccan propolis." Journal of Food Processing and Preservation , no. : e15816.

Review article
Published: 26 July 2021 in Environmental Science and Pollution Research
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It has recently been proven that epigenetic dysregulation is importantly involved in cell transformation and therefore induces cancerous diseases. The development of molecules called epidrugs, which target specifically different epigenetic modifications to restore cellular memory and therefore the treatment, became a real challenge currently. Currently, bioactive compounds of medicinal plants as epidrugs have been can identified and explored in cancer therapy. Indeed, these molecules can target specifically different epigenetic modulators including DNMT, HDAC, HAT, and HMT. Moreover, some compounds exhibit stochastic epigenetic actions on different pathways regulating cell memory. In this work, pharmacodynamic actions of natural epidrugs belonging to cannabinoids, carotenoids, chalcones, fatty acids, lignans, polysaccharides, saponins, secoiridoids, steroids, tannins, tanshinones, and other chemical classes we reported and highlighted. In this review, the effects of several natural bioactive compounds of epigenetic medications on cancerous diseases were highlighted. Numerous active molecules belonging to different chemical classes such as cannabinoids, carotenoids, fatty acids, lignans, polysaccharides, saponins, secoiridoids, steroids, tannins, and tanshinones are discussed in this review.

ACS Style

Nasreddine El Omari; Mohamed Bakha; Hamada Imtara; Fatima-Ezzahrae Guaouguaoua; Abdelaali Balahbib; Gokhan Zengin; Abdelhakim Bouyahya. Anticancer mechanisms of phytochemical compounds: focusing on epigenetic targets. Environmental Science and Pollution Research 2021, 1 -35.

AMA Style

Nasreddine El Omari, Mohamed Bakha, Hamada Imtara, Fatima-Ezzahrae Guaouguaoua, Abdelaali Balahbib, Gokhan Zengin, Abdelhakim Bouyahya. Anticancer mechanisms of phytochemical compounds: focusing on epigenetic targets. Environmental Science and Pollution Research. 2021; ():1-35.

Chicago/Turabian Style

Nasreddine El Omari; Mohamed Bakha; Hamada Imtara; Fatima-Ezzahrae Guaouguaoua; Abdelaali Balahbib; Gokhan Zengin; Abdelhakim Bouyahya. 2021. "Anticancer mechanisms of phytochemical compounds: focusing on epigenetic targets." Environmental Science and Pollution Research , no. : 1-35.

Journal article
Published: 24 July 2021 in Antioxidants
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In the current study, Achillea santolinoides and Achillea aleppica aeral parts and root were extracted with ethyl acetate, methanol, and water. Detailed phytochemical profiles were obtained using UHPLC-MS, yielding the identification of hydroxybenzoic and hydroxycinnamic acids, phenolic acid glycosides and sugar esters, acylquinic acids, O-glycosyl flavones and flavonols, and flavonoid aglycons, among others. The antioxidant properties and enzyme inhibitory activities of the extracts were assayed with in vitro tests. The phenolic content of the water extracts was significantly higher as compared to the ethyl acetate and methanol ones. A. aleppica aerial parts methanol extract possessed highest flavonoid content (49.18 mg rutin equivalent/g). Antioxidant properties assessment revealed that the methanol extract of A. santolinoides roots actively scavenged DPPH (54.11 mg TE/g) and ABTS radicals (112.53 mg TE/g) and possessed highest reducing potential (183.55 and 129.92 mg TE/g, for CUPRAC and FRAP, respectively). The ethyl acetate extracts of aerial parts and roots of both species showed highest inhibition against BuCHE (6.07–6.76 mg GALAE/g). The ethyl acetate extract of A.santolinoides aerial part showed highest inhibition against tyrosinase (73.00 mg KAE/g). These results showed that the tested Achillea species might represent novel phytotherapeutic avenues for the management of Alzheimer’s disease and epidermal hyperpigmentation conditions, which are both associated with oxidative stress. This paper could shed light into future potential industrial applications using the tested Achillea species.

ACS Style

Reneta Gevrenova; Gokhan Zengin; Kouadio Ibrahime Sinan; Evren Yıldıztugay; Dimitrina Zheleva-Dimitrova; Carene Picot-Allain; Mohamad Fawzi Mahomoodally; Muhammad Imran; Stefano Dall’Acqua. UHPLC-MS Characterization and Biological Insights of Different Solvent Extracts of Two Achillea Species (A. aleppica and A. santolinoides) from Turkey. Antioxidants 2021, 10, 1180 .

AMA Style

Reneta Gevrenova, Gokhan Zengin, Kouadio Ibrahime Sinan, Evren Yıldıztugay, Dimitrina Zheleva-Dimitrova, Carene Picot-Allain, Mohamad Fawzi Mahomoodally, Muhammad Imran, Stefano Dall’Acqua. UHPLC-MS Characterization and Biological Insights of Different Solvent Extracts of Two Achillea Species (A. aleppica and A. santolinoides) from Turkey. Antioxidants. 2021; 10 (8):1180.

Chicago/Turabian Style

Reneta Gevrenova; Gokhan Zengin; Kouadio Ibrahime Sinan; Evren Yıldıztugay; Dimitrina Zheleva-Dimitrova; Carene Picot-Allain; Mohamad Fawzi Mahomoodally; Muhammad Imran; Stefano Dall’Acqua. 2021. "UHPLC-MS Characterization and Biological Insights of Different Solvent Extracts of Two Achillea Species (A. aleppica and A. santolinoides) from Turkey." Antioxidants 10, no. 8: 1180.

Journal article
Published: 24 July 2021 in Journal of Pharmaceutical and Biomedical Analysis
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This study was aimed at providing a comprehensive phytochemical characterization and multi-biological assessment of Symphytum officinale L., a medicinal plant with a noteworthy traditional use, and Anchusa ochroleuca M. Bieb., a Boraginaceae species from the Romanian flora. The dichloromethane, methanol and 65 % ethanol extracts obtained from the roots and aerial parts of both plants revealed the presence of numerous phenolic acids, oxygenated fatty acids, pyrrolizidine alkaloids (PAs) and flavonoids, as assessed by LC-HRMS/MS analysis. Consistent with their higher total phenolic content, the polar aerial part extracts of S. officinale and root extracts of A. ochroleuca showed the most significant antioxidant activities, as evaluated by DPPH (173.22–216.98 mg TE/g) and ABTS (219.41–311.97 mg TE/g) radical scavenging, CUPRAC (387.18–626.40 mg TE/g), FRAP (199.36–299.86 mg TE/g) and total antioxidant capacity (2.28–2.68 mmol TE/g). Furthermore, both plants exhibited good tyrosinase (19.11–43.89 mg KAE/g) and α-glucosidase (2.45–12.54 mmol ACAE/g) inhibitory effects. The orthogonal projections to latent structures discriminant analysis (OPLS-DA) allowed the objective differentiation between the roots and aerial parts of the two investigated species based on their phytochemical and biological profiles. The partial least square (PLS) analysis showed that several individual phenolic acids, such as danshensu, rabdosiin and rosmarinic acid, significantly contributed to the antioxidant potential of both Boraginaceae species, whilst the relative levels of sucrose were positively correlated with the anti-enzymatic properties. Overall, S. officinale and A. ochroleuca could be regarded as rich sources of bioactive phytochemicals that could further lead to developing novel phyto-pharmaceutical commodities.

ACS Style

Adriana Trifan; Gokhan Zengin; Kouadio Ibrahime Sinan; Evelyn Wolfram; Krystyna Skalicka-Woźniak; Simon Vlad Luca. LC-HRMS/MS phytochemical profiling of Symphytum officinale L. and Anchusa ochroleuca M. Bieb. (Boraginaceae): Unveiling their multi-biological potential via an integrated approach. Journal of Pharmaceutical and Biomedical Analysis 2021, 204, 114283 .

AMA Style

Adriana Trifan, Gokhan Zengin, Kouadio Ibrahime Sinan, Evelyn Wolfram, Krystyna Skalicka-Woźniak, Simon Vlad Luca. LC-HRMS/MS phytochemical profiling of Symphytum officinale L. and Anchusa ochroleuca M. Bieb. (Boraginaceae): Unveiling their multi-biological potential via an integrated approach. Journal of Pharmaceutical and Biomedical Analysis. 2021; 204 ():114283.

Chicago/Turabian Style

Adriana Trifan; Gokhan Zengin; Kouadio Ibrahime Sinan; Evelyn Wolfram; Krystyna Skalicka-Woźniak; Simon Vlad Luca. 2021. "LC-HRMS/MS phytochemical profiling of Symphytum officinale L. and Anchusa ochroleuca M. Bieb. (Boraginaceae): Unveiling their multi-biological potential via an integrated approach." Journal of Pharmaceutical and Biomedical Analysis 204, no. : 114283.

Journal article
Published: 23 July 2021 in Nutrients
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In animals it has been demonstrated that Saccharomyces boulardii and Superoxide Dismutase (SOD) decrease low-grade inflammation and that S. boulardii can also decrease adiposity. The purpose of this study was to evaluate the effect of a 60-day S. boulardii and SOD supplementation on circulating markers of inflammation, body composition, hunger sensation, pro/antioxidant ratio, hormonal, lipid profile, glucose, insulin and HOMA-IR, in obese adults (BMI 30–35 kg/m2). Twenty-five obese adults were randomly assigned to intervention (8/4 women/men, 57 ± 8 years) or Placebo (9/4 women/men, 50 ± 9 years). Intervention group showed a statistically significant (p< 0.05) decrease of body weight, BMI, fat mass, insulin, HOMA Index and uric acid. Patients in intervention and control groups showed a significant decrease (p< 0.05) of GLP-1. Intervention group showed an increase (p< 0.05) of Vitamin D as well. In conclusion, the 60-day S. boulardii-SOD supplementation in obese subjects determined a significant weight loss with consequent decrease on fat mass, with preservation of fat free mass. The decrease of HOMA index and uric acid, produced additional benefits in obesity management. The observed increase in vitamin D levels in treated group requires further investigation.

ACS Style

Mariangela Rondanelli; Niccolò Miraglia; Pietro Putignano; Ignazio Castagliuolo; Paola Brun; Stefano Dall’Acqua; Gabriella Peroni; Milena Faliva; Maurizio Naso; Mara Nichetti; Vittoria Infantino; Simone Perna. Effects of 60-Day Saccharomyces boulardii and Superoxide Dismutase Supplementation on Body Composition, Hunger Sensation, Pro/Antioxidant Ratio, Inflammation and Hormonal Lipo-Metabolic Biomarkers in Obese Adults: A Double-Blind, Placebo-Controlled Trial. Nutrients 2021, 13, 2512 .

AMA Style

Mariangela Rondanelli, Niccolò Miraglia, Pietro Putignano, Ignazio Castagliuolo, Paola Brun, Stefano Dall’Acqua, Gabriella Peroni, Milena Faliva, Maurizio Naso, Mara Nichetti, Vittoria Infantino, Simone Perna. Effects of 60-Day Saccharomyces boulardii and Superoxide Dismutase Supplementation on Body Composition, Hunger Sensation, Pro/Antioxidant Ratio, Inflammation and Hormonal Lipo-Metabolic Biomarkers in Obese Adults: A Double-Blind, Placebo-Controlled Trial. Nutrients. 2021; 13 (8):2512.

Chicago/Turabian Style

Mariangela Rondanelli; Niccolò Miraglia; Pietro Putignano; Ignazio Castagliuolo; Paola Brun; Stefano Dall’Acqua; Gabriella Peroni; Milena Faliva; Maurizio Naso; Mara Nichetti; Vittoria Infantino; Simone Perna. 2021. "Effects of 60-Day Saccharomyces boulardii and Superoxide Dismutase Supplementation on Body Composition, Hunger Sensation, Pro/Antioxidant Ratio, Inflammation and Hormonal Lipo-Metabolic Biomarkers in Obese Adults: A Double-Blind, Placebo-Controlled Trial." Nutrients 13, no. 8: 2512.

Journal article
Published: 22 July 2021 in Molecules
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Prunus mahaleb L. fruit has long been used in the production of traditional liqueurs. The fruit also displayed scavenging and reducing activity, in vitro. The present study focused on unravelling peripheral and central protective effects, antimicrobial but also anti-COVID-19 properties exerted by the water extract of P. mahaleb. Anti-inflammatory effects were studied in isolated mouse colons exposed to lipopolysaccharide. Neuroprotection, measured as a blunting effect on hydrogen-peroxide-induced dopamine turnover, was investigated in hypothalamic HypoE22 cells. Antimicrobial effects were tested against different Gram+ and Gram- bacterial strains. Whereas anti-COVID-19 activity was studied in lung adenocarcinoma H1299 cells, where the gene expression of ACE2 and TMPRSS2 was measured after extract treatment. The bacteriostatic effects induced on Gram+ and Gram- strains, together with the inhibition of COX-2, TNFα, HIF1α, and VEGFA in the colon, suggest the potential of P. mahaleb water extract in contrasting the clinical symptoms related to ulcerative colitis. The inhibition of the hydrogen peroxide-induced DOPAC/DA ratio indicates promising neuroprotective effects. Finally, the downregulation of the gene expression of ACE2 and TMPRSS2 in H1299 cells, suggests the potential to inhibit SARS-CoV-2 virus entry in the human host. Overall, the results support the valorization of the local cultivation of P. mahaleb.

ACS Style

Giustino Orlando; Annalisa Chiavaroli; Sabrina Adorisio; Domenico Delfino; Luigi Brunetti; Lucia Recinella; Sheila Leone; Gokhan Zengin; Alessandra Acquaviva; Paola Angelini; Giancarlo Flores; Roberto Venanzoni; Simonetta Di Simone; Francesca Di Corpo; Andrei Mocan; Luigi Menghini; Claudio Ferrante. Unravelling the Phytochemical Composition and the Pharmacological Properties of an Optimized Extract from the Fruit from Prunus mahaleb L.: From Traditional Liqueur Market to the Pharmacy Shelf. Molecules 2021, 26, 4422 .

AMA Style

Giustino Orlando, Annalisa Chiavaroli, Sabrina Adorisio, Domenico Delfino, Luigi Brunetti, Lucia Recinella, Sheila Leone, Gokhan Zengin, Alessandra Acquaviva, Paola Angelini, Giancarlo Flores, Roberto Venanzoni, Simonetta Di Simone, Francesca Di Corpo, Andrei Mocan, Luigi Menghini, Claudio Ferrante. Unravelling the Phytochemical Composition and the Pharmacological Properties of an Optimized Extract from the Fruit from Prunus mahaleb L.: From Traditional Liqueur Market to the Pharmacy Shelf. Molecules. 2021; 26 (15):4422.

Chicago/Turabian Style

Giustino Orlando; Annalisa Chiavaroli; Sabrina Adorisio; Domenico Delfino; Luigi Brunetti; Lucia Recinella; Sheila Leone; Gokhan Zengin; Alessandra Acquaviva; Paola Angelini; Giancarlo Flores; Roberto Venanzoni; Simonetta Di Simone; Francesca Di Corpo; Andrei Mocan; Luigi Menghini; Claudio Ferrante. 2021. "Unravelling the Phytochemical Composition and the Pharmacological Properties of an Optimized Extract from the Fruit from Prunus mahaleb L.: From Traditional Liqueur Market to the Pharmacy Shelf." Molecules 26, no. 15: 4422.

Review
Published: 21 July 2021 in Environmental Science and Pollution Research
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Human papillomavirus (HPV) is a well-known sexually transmitted disorder globally. Human papillomavirus (HPV) is the 3rd most common cancer that causes cervical carcinoma, and globally it accounts for 275,000 deaths every year. The load of HPV-associated abrasions can be lessened through vaccination. At present, three forms of prophylactic vaccines, Cervarix, Gadrasil, and Gardasil 9, are commercially accessible but all these prophylactic vaccines have not the ability to manage and control developed abrasions or infections. Therefore, a considerable amount of the population is not secured from HPV infectivity. Consequently, the development of therapeutic HPV vaccines is a crucial requirement of this era, for the treatment of persisting infections, and to stop the progression of HPV-associated cancers. Therapeutic vaccines are a developing trial approach. Because of the constitutive expression of E6 and E7 early genes in cancerous and pre-cancerous tissues, and their involvement in disturbance of the cell cycle, these are best targets for this therapeutic vaccine treatment. For the synthesis and development of therapeutic vaccines, various approaches have been examined comprising cell-based vaccines, peptide/protein-based vaccines, nucleic acid-based vaccines, and live-vector vaccines all proceeding towards clinical trials. This review emphasizes the development, progress, current status, and future perspective of several vaccines for the cure of HPV-related abrasions and cancers. This review also provides an insight to assess the effectiveness, safety, efficacy, and immunogenicity of therapeutic vaccines in the cure of patients infected with HPV-associated cervical cancer.

ACS Style

Noor Ayesha; Sara Aboulaghras; Muhammad Jahangeer; Areej Riasat; Rehana Ramzan; Rameen Fatima; Muhammad Akram; Abdelaali Balahbib; Abdelhakim Bouyahya; Ekaterina Sepiashvili; Gokhan Zengin; Mohammad Ali Shariati. Physiopathology and effectiveness of therapeutic vaccines against human papillomavirus. Environmental Science and Pollution Research 2021, 1 .

AMA Style

Noor Ayesha, Sara Aboulaghras, Muhammad Jahangeer, Areej Riasat, Rehana Ramzan, Rameen Fatima, Muhammad Akram, Abdelaali Balahbib, Abdelhakim Bouyahya, Ekaterina Sepiashvili, Gokhan Zengin, Mohammad Ali Shariati. Physiopathology and effectiveness of therapeutic vaccines against human papillomavirus. Environmental Science and Pollution Research. 2021; ():1.

Chicago/Turabian Style

Noor Ayesha; Sara Aboulaghras; Muhammad Jahangeer; Areej Riasat; Rehana Ramzan; Rameen Fatima; Muhammad Akram; Abdelaali Balahbib; Abdelhakim Bouyahya; Ekaterina Sepiashvili; Gokhan Zengin; Mohammad Ali Shariati. 2021. "Physiopathology and effectiveness of therapeutic vaccines against human papillomavirus." Environmental Science and Pollution Research , no. : 1.

Journal article
Published: 16 July 2021 in Plants
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The quest for sustainable strategies aimed at increasing the bioactive properties of plant-based foods has grown quickly. In this work, we investigated the impact of exogenously applied phenolics, i.e., chlorogenic acid (CGA), hesperidin (HES), and their combinations (HES + CGA), on Lactuca sativa L. grown under normal- and mild-salinity conditions. To this aim, the phenolic profile, antioxidant properties, and enzyme inhibitory activity were determined. The untargeted metabolomics profiling revealed that lettuce treated with CGA under non-stressed conditions exhibited the highest total phenolic content (35.98 mg Eq./g). Lettuce samples grown under salt stress showed lower phenolic contents, except for lettuce treated with HES or HES + CGA, when comparing the same treatment between the two conditions. Furthermore, the antioxidant capacity was investigated through DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonate)), and FRAP (ferric reducing antioxidant power) assays, coupled with metal-chelating activity and phosphomolybdenum capacity. An exciting increase in radical scavenging capacity was observed in lettuce treated with exogenous phenolics, in both stress and non-stress conditions. The inhibitory activity of the samples was evaluated against target health-related enzymes, namely cholinesterases (acetylcholinesterase; AChE; butyryl cholinesterase; BChE), tyrosinase, α-amylase, and α-glucosidase. Lettuce treated with HES + CGA under non-stress conditions exhibited the strongest inhibition against AChE and BChE, while the same treatment under salinity conditions resulted in the highest inhibition capacity against α-amylase. Additionally, CGA under non-stress conditions exhibited the best inhibitory effect against tyrosinase. All the functional traits investigated were significantly modulated by exogenous phenolics, salinity, and their combination. In more detail, flavonoids, lignans, and stilbenes were the most affected phenolics, whereas glycosidase enzymes and tyrosinase activity were the most affected among enzyme assays. In conclusion, the exogenous application of phenolics to lettuce represents an effective and green strategy to effectively modulate the phenolic profile, antioxidant activity, and enzyme inhibitory effects in lettuce, deserving future application to produce functional plant-based foods in a sustainable way.

ACS Style

Leilei Zhang; Erika Martinelli; Biancamaria Senizza; Begoña Miras-Moreno; Evren Yildiztugay; Busra Arikan; Fevzi Elbasan; Gunes Ak; Melike Balci; Gokhan Zengin; Youssef Rouphael; Luigi Lucini. The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce. Plants 2021, 10, 1457 .

AMA Style

Leilei Zhang, Erika Martinelli, Biancamaria Senizza, Begoña Miras-Moreno, Evren Yildiztugay, Busra Arikan, Fevzi Elbasan, Gunes Ak, Melike Balci, Gokhan Zengin, Youssef Rouphael, Luigi Lucini. The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce. Plants. 2021; 10 (7):1457.

Chicago/Turabian Style

Leilei Zhang; Erika Martinelli; Biancamaria Senizza; Begoña Miras-Moreno; Evren Yildiztugay; Busra Arikan; Fevzi Elbasan; Gunes Ak; Melike Balci; Gokhan Zengin; Youssef Rouphael; Luigi Lucini. 2021. "The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce." Plants 10, no. 7: 1457.

Journal article
Published: 16 July 2021 in International Journal of Molecular Sciences
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This study focused on the biological evaluation and chemical characterization of Geranium pyrenaicum Burm. f. Different solvent extracts (hexane, ethyl acetate, methanol, and water extracts) were prepared. The phytochemical profile, antioxidant, and enzyme inhibitory activity were investigated. Cytotoxicity was assessed using VERO, FaDu, HeLa and RKO cells. The antiviral activity was carried out against HSV-1 (Herpes simplex virus 1) propagated in VERO cell line. The aqueous extract, possessing high phenolic content (170.50 mg gallic acid equivalent/g extract), showed the highest reducing capacity (613.27 and 364.10 mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant power, respectively), radical scavenging potential (469.82 mg Trolox equivalent/g extract, against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), metal chelating ability (52.39 mg ethylenediaminetetraacetic acid equivalent/g extract) and total antioxidant capacity (3.15 mmol Trolox equivalent/g extract). Liquid chromatography-electrospray ionization-quadrupole time-of-flight-mass spectrometry (LC-ESI-QTOF-MS/MS) alloved to tentatively identify a total of 56 compounds in the extracts, including ellagitannins, gallic acid and galloyl derivatives amongst others. The ethyl acetate extracts substantially depressed cholinesterase enzymes (4.49 and 12.26 mg galantamine equivalent/g extract against AChE and BChE, respectively) and α-amylase enzyme (1.04 mmol acarbose equivalent/g extract). On the other hand, the methanolic extract inhibited tyrosinase (121.42 mg kojic acid equivalent/g extract) and α-glucosidase (2.39 mmol acarbose equivalent/g extract) activities. The highest selectivity towards all cancer cell lines (SI 4.5–10.8) was observed with aqueous extract with the FaDu cells being the most sensitive (CC50 40.22 µg/mL). It can be concluded that the presence of certain bioactive antiviral molecules may be related to the high anti HSV-1 activity of the methanolic extract. This work has generated vital scientific data on this medicinal plant, which is a prospective candidate for the creation of innovative phyto-pharmaceuticals.

ACS Style

Łukasz Świątek; Elwira Sieniawska; Kouadio Sinan; Magdalena Maciejewska-Turska; Anastazja Boguszewska; Małgorzata Polz-Dacewicz; Ismail Senkardes; Gokalp Guler; Nabeelah Bibi Sadeer; Mohamad Mahomoodally; Gokhan Zengin. LC-ESI-QTOF-MS/MS Analysis, Cytotoxic, Antiviral, Antioxidant, and Enzyme Inhibitory Properties of Four Extracts of Geranium pyrenaicum Burm. f.: A Good Gift from the Natural Treasure. International Journal of Molecular Sciences 2021, 22, 7621 .

AMA Style

Łukasz Świątek, Elwira Sieniawska, Kouadio Sinan, Magdalena Maciejewska-Turska, Anastazja Boguszewska, Małgorzata Polz-Dacewicz, Ismail Senkardes, Gokalp Guler, Nabeelah Bibi Sadeer, Mohamad Mahomoodally, Gokhan Zengin. LC-ESI-QTOF-MS/MS Analysis, Cytotoxic, Antiviral, Antioxidant, and Enzyme Inhibitory Properties of Four Extracts of Geranium pyrenaicum Burm. f.: A Good Gift from the Natural Treasure. International Journal of Molecular Sciences. 2021; 22 (14):7621.

Chicago/Turabian Style

Łukasz Świątek; Elwira Sieniawska; Kouadio Sinan; Magdalena Maciejewska-Turska; Anastazja Boguszewska; Małgorzata Polz-Dacewicz; Ismail Senkardes; Gokalp Guler; Nabeelah Bibi Sadeer; Mohamad Mahomoodally; Gokhan Zengin. 2021. "LC-ESI-QTOF-MS/MS Analysis, Cytotoxic, Antiviral, Antioxidant, and Enzyme Inhibitory Properties of Four Extracts of Geranium pyrenaicum Burm. f.: A Good Gift from the Natural Treasure." International Journal of Molecular Sciences 22, no. 14: 7621.

Journal article
Published: 28 June 2021 in Antibiotics
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Larch (Larix decidua) bark is a sawmill waste, traditionally used for antiseptic, expectorant and dermatological (wound healing, eczema, psoriasis) purposes. In this work, we developed a food-grade dry larch bark extract (LBE) from sawmill by-products using hydro-alcoholic extraction. The antibacterial activity of LBE was evaluated against respiratory-tract pathogens, i.e., Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa and Haemophilus influenza, and it was compared to that of grapefruit seed extract (GSE), a commercially available raw material commonly proposed as antibacterial ingredient for over-the-counter products. Procyanidins (PACs) and other polyphenols contents in LBE were determined by HPLC-FLD-MS and HPLC-DAD-MSn, respectively. The antimicrobial activity of LBE and GSE was assessed using the micro-plate dilution technique in concentration range of 2–200 µg/mL, and the safety of these dosages was assessed in cellular and animal models. LBE showed considerable contents of PACs (15% w/w; especially B-type) and other polyphenols (3.8% w/w), among which the characteristic spiropolyphenols larixinol and epilarixinol were identified, together with the flavonoids isoquercitrin and rutin, already reported as growth inhibitors of different respiratory-tract pathogens. LBE showed higher antimicrobial activity compared to GSE, demonstrated by a growth inhibition range of 10–40% towards five of six strains tested, compared to 10–15% of GSE. These results suggest that LBE may represent a natural and sustainable source of active compounds with antibacterial activity for pharmaceutical and nutraceutical applications.

ACS Style

Marta Faggian; Giulia Bernabè; Sara Ferrari; Stefano Francescato; Gianni Baratto; Ignazio Castagliuolo; Stefano Dall’Acqua; Gregorio Peron. Polyphenol-Rich Larix decidua Bark Extract with Antimicrobial Activity against Respiratory-Tract Pathogens: A Novel Bioactive Ingredient with Potential Pharmaceutical and Nutraceutical Applications. Antibiotics 2021, 10, 789 .

AMA Style

Marta Faggian, Giulia Bernabè, Sara Ferrari, Stefano Francescato, Gianni Baratto, Ignazio Castagliuolo, Stefano Dall’Acqua, Gregorio Peron. Polyphenol-Rich Larix decidua Bark Extract with Antimicrobial Activity against Respiratory-Tract Pathogens: A Novel Bioactive Ingredient with Potential Pharmaceutical and Nutraceutical Applications. Antibiotics. 2021; 10 (7):789.

Chicago/Turabian Style

Marta Faggian; Giulia Bernabè; Sara Ferrari; Stefano Francescato; Gianni Baratto; Ignazio Castagliuolo; Stefano Dall’Acqua; Gregorio Peron. 2021. "Polyphenol-Rich Larix decidua Bark Extract with Antimicrobial Activity against Respiratory-Tract Pathogens: A Novel Bioactive Ingredient with Potential Pharmaceutical and Nutraceutical Applications." Antibiotics 10, no. 7: 789.

Review
Published: 28 June 2021 in Food Reviews International
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Camphene (C10H16) also-called2,2-dimethyl-3-methylidenebicyclo[2.2.1] heptane is a volatile compound belonging to the terpenoid family, in particular to the group of monoterpene hydrocarbons. It is presented as a secondary metabolite in various aromatic and medicinal plants, especially Thymus, Origanum, and Salvia genera, and is considered one of the major components of their essential oils. Numerous in vitro and in vivo investigations have proven the biological properties of camphene including antibacterial, antifungal, anticancer, antioxidant, antiparasitic, antidiabetic, anti-inflammatory, and hypolipidemic activities. Moreover, camphene was also reported to exhibit anti-leishmanial, hepatoprotective, antiviral, and anti-acetylcholinesterase inhibitory activities. The current review highlights the previous reports on the biological properties of this molecule; camphene. The mechanisms of action involved and the potential application of camphene as a drug are also discussed. In light of these findings, in-depth preclinical and clinical studies on this molecule are strongly required to validate its applications in pharmaceutical field. Presented information could shed light into further studies on this molecule.

ACS Style

Naoufal EL Hachlafi; Tariq Aanniz; Naoual El Menyiy; Aicha El Baaboua; Nasreddine El Omari; Abdelaali Balahbib; Mohammad Ali Shariati; Gokhan Zengin; Kawtar Fikri-Benbrahim; Abdelhakim Bouyahya. In Vitro and in Vivo Biological Investigations of Camphene and Its Mechanism Insights: A Review. Food Reviews International 2021, 1 -28.

AMA Style

Naoufal EL Hachlafi, Tariq Aanniz, Naoual El Menyiy, Aicha El Baaboua, Nasreddine El Omari, Abdelaali Balahbib, Mohammad Ali Shariati, Gokhan Zengin, Kawtar Fikri-Benbrahim, Abdelhakim Bouyahya. In Vitro and in Vivo Biological Investigations of Camphene and Its Mechanism Insights: A Review. Food Reviews International. 2021; ():1-28.

Chicago/Turabian Style

Naoufal EL Hachlafi; Tariq Aanniz; Naoual El Menyiy; Aicha El Baaboua; Nasreddine El Omari; Abdelaali Balahbib; Mohammad Ali Shariati; Gokhan Zengin; Kawtar Fikri-Benbrahim; Abdelhakim Bouyahya. 2021. "In Vitro and in Vivo Biological Investigations of Camphene and Its Mechanism Insights: A Review." Food Reviews International , no. : 1-28.

Journal article
Published: 25 June 2021 in Molecules
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In the present study the ability of supercritical carbon dioxide (SCO2) extracts of M. longifolia L. leaves to modulate low-density lipoprotein receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9) expression was evaluated in cultured human hepatoma cell lines Huh7 and HepG2. Two SCO2 extracts, one oil (ML-SCO2) and a semisolid (MW-SCO2), were subjected to detailed chemical characterization by mono- and bidimensional nuclear magnetic resonance (1D, 2D-NMR), gas chromatography coupled with mass spectrometry (GC-MS) and liquid chromatography coupled with mass spectrometry (LC-MS). Chemical analysis revealed significant amounts of fatty acids, phytosterols and terpenoids. ML-SCO2 was able to induce LDLR expression at a dose of 60 µg/mL in HuH7 and HepG2 cell lines. Furthermore, ML-SCO2 reduced PCSK9 secretion in a concentration-dependent manner in both cell lines. Piperitone oxide, the most abundant compound of the volatile constituent of ML-SCO2 (27% w/w), was isolated and tested for the same targets, showing a very effective reduction of PCSK9 expression. The overall results revealed the opportunity to obtain a new nutraceutical ingredient with a high amount of phytosterols and terpenoids using the SCO2 extraction of M. longifolia L., a very well-known botanical species used as food. Furthermore, for the first time we report the high activity of piperitone oxide in the reduction of PCSK9 expression.

ACS Style

Stefania Sut; Irene Ferrarese; Maria Lupo; Nicola De Zordi; Elisa Tripicchio; Nicola Ferri; Stefano Dall’ Acqua. The Modulation of PCSK9 and LDLR by Supercritical CO2 Extracts of Mentha longifolia and Isolated Piperitone Oxide, an In Vitro Study. Molecules 2021, 26, 3886 .

AMA Style

Stefania Sut, Irene Ferrarese, Maria Lupo, Nicola De Zordi, Elisa Tripicchio, Nicola Ferri, Stefano Dall’ Acqua. The Modulation of PCSK9 and LDLR by Supercritical CO2 Extracts of Mentha longifolia and Isolated Piperitone Oxide, an In Vitro Study. Molecules. 2021; 26 (13):3886.

Chicago/Turabian Style

Stefania Sut; Irene Ferrarese; Maria Lupo; Nicola De Zordi; Elisa Tripicchio; Nicola Ferri; Stefano Dall’ Acqua. 2021. "The Modulation of PCSK9 and LDLR by Supercritical CO2 Extracts of Mentha longifolia and Isolated Piperitone Oxide, an In Vitro Study." Molecules 26, no. 13: 3886.

Journal article
Published: 18 June 2021 in Molecules
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Diclinanona calycina R. E. Fries popularly known as “envira”, is a species of the Annonaceae family endemic to Brazil. In our ongoing search for bioactive compounds from Annonaceae Amazon plants, the bark of D. calycina was investigated by classical chromatography techniques that yielded thirteen compounds (alkaloids and flavonoids) described for the first time in D. calycina as well as in the genus Diclinanona. The structure of these isolated compounds were established by extensive analysis using 1D/2D-NMR spectroscopy in combination with MS. The isolated alkaloids were identified as belonging to the subclasses: simple isoquinoline, thalifoline (1); aporphine, anonaine (2); oxoaporphine, liriodenine (3); benzyltetrahydroisoquinolines, (S)-(+)-reticuline (4); dehydro-oxonorreticuline (3,4-dihydro-7-hydroxy-6-methoxy-1-isoquinolinyl)(3-hydroxy-4-methoxyphenyl)-methanone) (5); (+)-1S,2R-reticuline Nβ-oxide (6); and (+)-1S,2S-reticuline Nα-oxide (7); tetrahydroprotoberberine, coreximine (8); and pavine, bisnorargemonine (9). While the flavonoids belong to the benzylated dihydroflavones, isochamanetin (10), dichamanetin (11), and a mixture of uvarinol (12) and isouvarinol (13). Compound 5 is described for the first time in the literature as a natural product. The cytotoxic activity of the main isolated compounds was evaluated against cancer and non-cancerous cell lines. Among the tested compounds, the most promising results were found for the benzylated dihydroflavones dichamanetin (10), and the mixture of uvarinol (12) and isouvarinol (13), which presented moderate cytotoxic activity against the tested cancer cell lines (25.0 µg·mL−1). Dichamanetin (11) showed cytotoxic activity against HL-60 and HCT116 with IC50 values of 15.78 µg·mL−1 (33.70 µmol·L−1) and 18.99 µg·mL−1 (40.56 µmol·L−1), respectively while the mixture of uvarinol (12) and isouvarinol (13) demonstrated cytotoxic activity against HL-60, with an IC50 value of 9.74 µg·mL−1, and HCT116, with an IC50 value of 17.31 µg·mL−1. These cytotoxic activities can be attributed to the presence of one or more hydroxybenzyl groups present in these molecules as well as the position in which these groups are linked. The cytotoxic activities of reticuline, anonaine and liriodenine have been previously established, with liriodenine being the most potent compound.

ACS Style

Emmanoel Costa; Liviane Soares; Jamal Chaar; Valdenizia Silva; Luciano Santos; Hector Koolen; Felipe Silva; Josean Tavares; Gokhan Zengin; Milena Soares; Daniel Bezerra. Benzylated Dihydroflavones and Isoquinoline-Derived Alkaloids from the Bark of Diclinanona calycina (Annonaceae) and Their Cytotoxicities. Molecules 2021, 26, 3714 .

AMA Style

Emmanoel Costa, Liviane Soares, Jamal Chaar, Valdenizia Silva, Luciano Santos, Hector Koolen, Felipe Silva, Josean Tavares, Gokhan Zengin, Milena Soares, Daniel Bezerra. Benzylated Dihydroflavones and Isoquinoline-Derived Alkaloids from the Bark of Diclinanona calycina (Annonaceae) and Their Cytotoxicities. Molecules. 2021; 26 (12):3714.

Chicago/Turabian Style

Emmanoel Costa; Liviane Soares; Jamal Chaar; Valdenizia Silva; Luciano Santos; Hector Koolen; Felipe Silva; Josean Tavares; Gokhan Zengin; Milena Soares; Daniel Bezerra. 2021. "Benzylated Dihydroflavones and Isoquinoline-Derived Alkaloids from the Bark of Diclinanona calycina (Annonaceae) and Their Cytotoxicities." Molecules 26, no. 12: 3714.