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Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive, versatile, and label-free. In this work, an SPR probe, based on a poly(methyl methacrylate) (PMMA) slab waveguide covered by a gold nanofilm, combined with a specific molecularly imprinted polymer (MIP) receptor for bovine serum albumin (BSA) protein, has been realized and experimentally characterized. The obtained experimental results have shown a limit of detection (LOD) equal to about 8.5 × 10−9 M. This value is smaller than the one achieved by another SPR probe, based on a D-shaped plastic optical fiber (POF), functionalized with the same MIP receptor; more specifically, the obtained LOD was reduced by about three orders of magnitude with respect to the POF configuration. Moreover, concerning the D-shaped POF configuration, no manufacturing process is present in the proposed sensor configuration. In addition, fibers are used only to connect the simple sensor chip with a light source and a detector, promoting a bio-chemical sensing approach based on disposable, low-cost, and removable chips.
Francesco Arcadio; Luigi Zeni; Chiara Perri; Girolamo D’Agostino; Giudo Chiaretti; Giovanni Porto; Aldo Minardo; Nunzio Cennamo. Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor. Chemosensors 2021, 9, 218 .
AMA StyleFrancesco Arcadio, Luigi Zeni, Chiara Perri, Girolamo D’Agostino, Giudo Chiaretti, Giovanni Porto, Aldo Minardo, Nunzio Cennamo. Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor. Chemosensors. 2021; 9 (8):218.
Chicago/Turabian StyleFrancesco Arcadio; Luigi Zeni; Chiara Perri; Girolamo D’Agostino; Giudo Chiaretti; Giovanni Porto; Aldo Minardo; Nunzio Cennamo. 2021. "Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor." Chemosensors 9, no. 8: 218.
In a specific biosensing application, a nanoplasmonic sensor chip has been tested by an experimental setup based on an aluminum holder and two plastic optical fibers used to illuminate and collect the transmitted light. The studied plasmonic probe is based on gold nanograting, realized on the top of a Poly(methyl methacrylate) (PMMA) chip. The PMMA substrate could be considered as a transparent substrate and, in such a way, it has been already used in previous work. Alternatively, here it is regarded as a slab waveguide. In particular, we have deposited upon the slab surface, covered with a nanograting, a synthetic receptor specific for bovine serum albumin (BSA), to test the proposed biosensing approach. Exploiting this different experimental configuration, we have determined how the orientation of the nanostripes forming the grating pattern, with respect to the direction of the input light (longitudinal or orthogonal), influences the biosensing performances. For example, the best limit of detection (LOD) in the BSA detection that has been obtained is equal to 23 pM. Specifically, the longitudinal configuration is characterized by two observable plasmonic phenomena, each sensitive to a different BSA concentration range, ranging from pM to µM. This aspect plays a key role in several biochemical sensing applications, where a wide working range is required.
Francesco Arcadio; Luigi Zeni; Aldo Minardo; Caterina Eramo; Stefania Di Ronza; Chiara Perri; Girolamo D’Agostino; Guido Chiaretti; Giovanni Porto; Nunzio Cennamo. A Nanoplasmonic-Based Biosensing Approach for Wide-Range and Highly Sensitive Detection of Chemicals. Nanomaterials 2021, 11, 1961 .
AMA StyleFrancesco Arcadio, Luigi Zeni, Aldo Minardo, Caterina Eramo, Stefania Di Ronza, Chiara Perri, Girolamo D’Agostino, Guido Chiaretti, Giovanni Porto, Nunzio Cennamo. A Nanoplasmonic-Based Biosensing Approach for Wide-Range and Highly Sensitive Detection of Chemicals. Nanomaterials. 2021; 11 (8):1961.
Chicago/Turabian StyleFrancesco Arcadio; Luigi Zeni; Aldo Minardo; Caterina Eramo; Stefania Di Ronza; Chiara Perri; Girolamo D’Agostino; Guido Chiaretti; Giovanni Porto; Nunzio Cennamo. 2021. "A Nanoplasmonic-Based Biosensing Approach for Wide-Range and Highly Sensitive Detection of Chemicals." Nanomaterials 11, no. 8: 1961.
In this work, a novel approach to realize a plasmonic sensor is presented. The proposed optical sensor device is designed, manufactured, and experimentally tested. Two photo-curable resins are used to 3D print a surface plasmon resonance (SPR) sensor. Both numerical and experimental analyses are presented in the paper. The numerical and experimental results confirm that the 3D printed SPR sensor presents performances, in term of figure of merit (FOM), very similar to other SPR sensors made using plastic optical fibers (POFs). For the 3D printed sensor, the measured FOM is 13.6 versus 13.4 for the SPR-POF configuration. The cost analysis shows that the 3D printed SPR sensor can be manufactured at low cost (∼15 €) that is competitive with traditional sensors. The approach presented here allows to realize an innovative SPR sensor showing low-cost, 3D-printing manufacturing free design and the feasibility to be integrated with other optical devices on the same plastic planar support, thus opening undisclosed future for the optical sensor systems.
Nunzio Cennamo; Lorena Saitta; Claudio Tosto; Francesco Arcadio; Luigi Zeni; Maria Fragalá; Gianluca Cicala. Microstructured Surface Plasmon Resonance Sensor Based on Inkjet 3D Printing Using Photocurable Resins with Tailored Refractive Index. Polymers 2021, 13, 2518 .
AMA StyleNunzio Cennamo, Lorena Saitta, Claudio Tosto, Francesco Arcadio, Luigi Zeni, Maria Fragalá, Gianluca Cicala. Microstructured Surface Plasmon Resonance Sensor Based on Inkjet 3D Printing Using Photocurable Resins with Tailored Refractive Index. Polymers. 2021; 13 (15):2518.
Chicago/Turabian StyleNunzio Cennamo; Lorena Saitta; Claudio Tosto; Francesco Arcadio; Luigi Zeni; Maria Fragalá; Gianluca Cicala. 2021. "Microstructured Surface Plasmon Resonance Sensor Based on Inkjet 3D Printing Using Photocurable Resins with Tailored Refractive Index." Polymers 13, no. 15: 2518.
We demonstrate the use of a graded-index perfluorinated optical fiber (GI-POF) for distributed static and dynamic strain measurements based on Rayleigh scattering. The system is based on an amplitude-based phase-sensitive Optical Time-Domain Reflectometry (ϕ-OTDR) configuration, operated at the unconventional wavelength of 850 nm. Static strain measurements have been carried out at a spatial resolution of 4 m and for a strain up to 3.5% by exploiting the increase of the backscatter Rayleigh coefficient consequent to the application of a tensile strain, while vibration/acoustic measurements have been demonstrated for a sampling frequency up to 833 Hz by exploiting the vibration-induced changes in the backscatter Rayleigh intensity time-domain traces arising from coherent interference within the pulse. The reported tests demonstrate that polymer optical fibers can be used for cost-effective multiparameter sensing.
Agnese Coscetta; Ester Catalano; Enis Cerri; Ricardo Oliveira; Lucia Bilro; Luigi Zeni; Nunzio Cennamo; Aldo Minardo. Distributed Static and Dynamic Strain Measurements in Polymer Optical Fibers by Rayleigh Scattering. Sensors 2021, 21, 5049 .
AMA StyleAgnese Coscetta, Ester Catalano, Enis Cerri, Ricardo Oliveira, Lucia Bilro, Luigi Zeni, Nunzio Cennamo, Aldo Minardo. Distributed Static and Dynamic Strain Measurements in Polymer Optical Fibers by Rayleigh Scattering. Sensors. 2021; 21 (15):5049.
Chicago/Turabian StyleAgnese Coscetta; Ester Catalano; Enis Cerri; Ricardo Oliveira; Lucia Bilro; Luigi Zeni; Nunzio Cennamo; Aldo Minardo. 2021. "Distributed Static and Dynamic Strain Measurements in Polymer Optical Fibers by Rayleigh Scattering." Sensors 21, no. 15: 5049.
A specific aptameric sequence has been immobilized on short polyethyleneglycol (PEG) interface on gold nano-film deposited on a D-shaped plastic optical fiber (POFs) probe, and the protein binding has been monitored exploiting the very sensitive surface plasmon resonance (SPR) phenomenon. The receptor-binding domain (RBD) of the SARS-CoV-2 spike glycoprotein has been specifically used to develop an aptasensor. Surface analysis techniques coupled to fluorescence microscopy and plasmonic analysis have been utilized to characterize the biointerface. Spanning a wide protein range (25 ÷ 1000 nM), the SARS-Cov-2 spike protein was detected with a Limit of Detection (LoD) of about 37 nM. Different interferents (BSA, AH1N1 hemagglutinin protein and MERS spike protein) have been tested confirming the specificity of our aptasensor. Finally, a preliminary test in diluted human serum encouraged its application in a point-of-care device, since POF-based aptasensor represent a potentially low-cost compact biosensor, characterized by a rapid response, a small size and could be an ideal laboratory portable diagnostic tool.
Nunzio Cennamo; Laura Pasquardini; Francesco Arcadio; Lorenzo Lunelli; Lia Vanzetti; Vincenzo Carafa; Lucia Altucci; Luigi Zeni. SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor. Talanta 2021, 233, 122532 -122532.
AMA StyleNunzio Cennamo, Laura Pasquardini, Francesco Arcadio, Lorenzo Lunelli, Lia Vanzetti, Vincenzo Carafa, Lucia Altucci, Luigi Zeni. SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor. Talanta. 2021; 233 ():122532-122532.
Chicago/Turabian StyleNunzio Cennamo; Laura Pasquardini; Francesco Arcadio; Lorenzo Lunelli; Lia Vanzetti; Vincenzo Carafa; Lucia Altucci; Luigi Zeni. 2021. "SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor." Talanta 233, no. : 122532-122532.
Postoperative pancreatic fistula (POPF), the major driver of morbidity and mortality following pancreatectomy, is caused by an abnormal communication between the pancreatic ductal epithelium and another epithelial surface containing pancreas-derived, enzyme-rich fluid. There is a strong correlation between the amylase content in surgically-placed drains early in the postoperative course and the development of POPF. A simple and cheap method to determine the amylase content from the drain effluent has been eagerly advocated. Here, we developed an amylase optical biosensor, based on a surface plasmon resonance (SPR) plastic optical fiber (POF), metallized with a 60 nm layer of gold and interrogated with white light. The sensor was made specific by coupling it with an anti-amylase antibody. Each surface derivatization step was optimized and studied by XPS, contact angle, and fluorescence. The POF-biosensor was tested for its response to amylase in diluted drain effluents. The volume of sample required was 50 µL and the measurement time was 8 min. The POF-biosensor showed selectivity for amylase, a calibration curve log-linear in the range of 0.8–25.8 U/L and a limit of detection (LOD) of ~0.5 U/L. In preliminary tests, the POF-biosensor allowed for the measurement of the amylase content of diluted surgically-placed drain effluents with an accuracy of >92% with respect to the gold standard. The POF-biosensor allows for reliable measurement and could be implemented to allow for a rapid bedside assessment of amylase value in drains following pancreatectomy.
Laura Pasquardini; Nunzio Cennamo; Giuseppe Malleo; Lia Vanzetti; Luigi Zeni; Deborah Bonamini; Roberto Salvia; Claudio Bassi; Alessandra Bossi. A Surface Plasmon Resonance Plastic Optical Fiber Biosensor for the Detection of Pancreatic Amylase in Surgically-Placed Drain Effluent. Sensors 2021, 21, 3443 .
AMA StyleLaura Pasquardini, Nunzio Cennamo, Giuseppe Malleo, Lia Vanzetti, Luigi Zeni, Deborah Bonamini, Roberto Salvia, Claudio Bassi, Alessandra Bossi. A Surface Plasmon Resonance Plastic Optical Fiber Biosensor for the Detection of Pancreatic Amylase in Surgically-Placed Drain Effluent. Sensors. 2021; 21 (10):3443.
Chicago/Turabian StyleLaura Pasquardini; Nunzio Cennamo; Giuseppe Malleo; Lia Vanzetti; Luigi Zeni; Deborah Bonamini; Roberto Salvia; Claudio Bassi; Alessandra Bossi. 2021. "A Surface Plasmon Resonance Plastic Optical Fiber Biosensor for the Detection of Pancreatic Amylase in Surgically-Placed Drain Effluent." Sensors 21, no. 10: 3443.
Nowadays there is an increasing request to realize green, eco-friendly and biodegradable electronic devices for biosensor implementation. In this context, we have conceived and realized a green sensor based on Localized Surface Plasmon Resonance (LSPR) phenomenon in a thin slab waveguide of Bacterial Cellulose (BC). These LSPR sensors can be obtained simply by gold sputtering on the slab BC waveguides. The performances have been studied investigating the presence of ionic liquids (ILs) inside and in absence of ILs with various thickness of the BC substrate. Depending of the thickness of the BC layer, the ILs effect on the LSPR can be constructive or destructive. In this work, we present a study of the sensor performances, in terms of bulk sensitivity and resolution by changing the aforementioned parameters. Analyses in terms of BC geometry are pursued in order to improve the interaction between the light and the LSPR phenomenon. The experimental setup used for this kind of extrinsic optical fiber LSPR sensor is based on two optical fibers used to connect a white light source and a spectrometer with the green LSPR sensor chip. Results evince the suitability of the proposed approach in order to realize sensors and biosensors with several intriguing properties and features. In fact, these LSPR platforms could be used to realize disposable biosensors, when a specific bioreceptor is covalently bonded to the gold.
Nunzio Cennamo; Carlo Trigona; Salvatore Graziani; Luigi Zeni; Francesco Arcadio; Liu Xiaoyan; Giovanna Di Pasquale; Antonino Pollicino. Green LSPR Sensors Based on Thin Bacterial Cellulose Waveguides for Disposable Biosensor Implementation. IEEE Transactions on Instrumentation and Measurement 2021, 70, 1 -8.
AMA StyleNunzio Cennamo, Carlo Trigona, Salvatore Graziani, Luigi Zeni, Francesco Arcadio, Liu Xiaoyan, Giovanna Di Pasquale, Antonino Pollicino. Green LSPR Sensors Based on Thin Bacterial Cellulose Waveguides for Disposable Biosensor Implementation. IEEE Transactions on Instrumentation and Measurement. 2021; 70 (99):1-8.
Chicago/Turabian StyleNunzio Cennamo; Carlo Trigona; Salvatore Graziani; Luigi Zeni; Francesco Arcadio; Liu Xiaoyan; Giovanna Di Pasquale; Antonino Pollicino. 2021. "Green LSPR Sensors Based on Thin Bacterial Cellulose Waveguides for Disposable Biosensor Implementation." IEEE Transactions on Instrumentation and Measurement 70, no. 99: 1-8.
In this work, an unconventional light-diffusing fiber (LDF) plasmonic platform is exploited to efficiently monitor a bio-interaction. The surface plasmon resonance (SPR)-LDF sensor is used to detect the immunoglobulin G (IgG)/anti-IgG interaction as exemplifying bioassay. The IgG bioreceptor was deposited on the gold surface of the SPR-LDF platform, and the biological target was transported through a customized thermo-stabilized flow cell by means of a buffer fluid. Moreover, to test the usability of the proposed SPR-LDF biosensor also for immunosensing in complex matrices, a second assay was conducted by immobilizing anti-C-reactive protein (CRP) and detecting different concentrations of CRP in human serum. An innovative data analysis approach for the extraction of the best value of the resonance wavelength was developed and applied. The obtained results reveal that the unconventional platform represented by the LDF can be successfully exploited for the implementation of SPR-based biosensors with very good performances, also when compared to more common SPR systems.
Nunzio Cennamo; Cosimo Trono; Ambra Giannetti; Francesco Baldini; Aldo Minardo; Luigi Zeni; Sara Tombelli. Biosensors exploiting unconventional platforms: The case of plasmonic light-diffusing fibers. Sensors and Actuators B: Chemical 2021, 337, 129771 .
AMA StyleNunzio Cennamo, Cosimo Trono, Ambra Giannetti, Francesco Baldini, Aldo Minardo, Luigi Zeni, Sara Tombelli. Biosensors exploiting unconventional platforms: The case of plasmonic light-diffusing fibers. Sensors and Actuators B: Chemical. 2021; 337 ():129771.
Chicago/Turabian StyleNunzio Cennamo; Cosimo Trono; Ambra Giannetti; Francesco Baldini; Aldo Minardo; Luigi Zeni; Sara Tombelli. 2021. "Biosensors exploiting unconventional platforms: The case of plasmonic light-diffusing fibers." Sensors and Actuators B: Chemical 337, no. : 129771.
A surface plasmon resonance (SPR) platform, based on a D-shaped plastic optical fiber (POF), combined with a biomimetic receptor, i.e., a molecularly imprinted polymer (MIP), is proposed to detect furfural (2-furaldheide, 2-FAL) in fermented beverages like wine. MIPs have been demonstrated to be a very convenient biomimetic receptor in the proposed sensing device, being easy and rapid to develop, suitable for on-site determinations at low concentrations, and cheap. Moreover, the MIP film thickness can be changed to modulate the sensing parameters. The possibility of performing single drop measurements is a further favorable aspect for practical applications. For example, the use of an SPR-MIP sensor for the analysis of 2-FAL in a real life matrix such as wine is proposed, obtaining a low detection limit of 0.004 mg L−1. The determination of 2-FAL in fermented beverages is becoming a crucial task, mainly for the effects of the furanic compounds on the flavor of food and their toxic and carcinogenic effect on human beings.
Maria Pesavento; Luigi Zeni; Letizia De Maria; Giancarla Alberti; Nunzio Cennamo. SPR-Optical Fiber-Molecularly Imprinted Polymer Sensor for the Detection of Furfural in Wine. Biosensors 2021, 11, 72 .
AMA StyleMaria Pesavento, Luigi Zeni, Letizia De Maria, Giancarla Alberti, Nunzio Cennamo. SPR-Optical Fiber-Molecularly Imprinted Polymer Sensor for the Detection of Furfural in Wine. Biosensors. 2021; 11 (3):72.
Chicago/Turabian StyleMaria Pesavento; Luigi Zeni; Letizia De Maria; Giancarla Alberti; Nunzio Cennamo. 2021. "SPR-Optical Fiber-Molecularly Imprinted Polymer Sensor for the Detection of Furfural in Wine." Biosensors 11, no. 3: 72.
The rapid spread of the Coronavirus Disease 2019 (COVID-19) pandemic, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pathogen has generated a huge international public health emergency. Currently the reference diagnostic technique for virus determination is Reverse Transcription Polymerase Chain Reaction (RT-PCR) real time analysis that requires specialized equipment, reagents and facilities and typically 3–4 h to perform. Thus, the realization of simple, low-cost, small-size, rapid and point-of-care diagnostics tests has become a global priority. In response to the current need for quick, highly sensitive and on-site detection of the SARS-CoV-2 virus in several aqueous solutions, a specific molecularly imprinted polymer (MIP) receptor has been designed, realized, and combined with an optical sensor. More specifically, the proof of concept of a SARS-CoV-2 sensor has been demonstrated by exploiting a plasmonic plastic optical fiber sensor coupled with a novel kind of synthetic MIP nano-layer, especially designed for the specific recognition of Subunit 1 of the SARS-CoV-2 Spike protein. First, we have tested the effectiveness of the developed MIP receptor to bind the Subunit 1 of the SARS-CoV-2 spike protein, then the results of preliminary tests on SARS-CoV-2 virions, performed on samples of nasopharyngeal (NP) swabs in universal transport medium (UTM) and physiological solution (0.9% NaCl), were compared with those obtained with RT-PCR. According to these preliminary results, the sensitivity of the proposed optical-chemical sensor proved to be higher than the RT-PCR one. Furthermore, a relatively fast response time (about 10 min) to the virus was obtained without the use of additional reagents.
Nunzio Cennamo; Girolamo D’Agostino; Chiara Perri; Francesco Arcadio; Guido Chiaretti; Eva Parisio; Giulio Camarlinghi; Chiara Vettori; Francesco Di Marzo; Rosario Cennamo; Giovanni Porto; Luigi Zeni. Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers. Sensors 2021, 21, 1681 .
AMA StyleNunzio Cennamo, Girolamo D’Agostino, Chiara Perri, Francesco Arcadio, Guido Chiaretti, Eva Parisio, Giulio Camarlinghi, Chiara Vettori, Francesco Di Marzo, Rosario Cennamo, Giovanni Porto, Luigi Zeni. Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers. Sensors. 2021; 21 (5):1681.
Chicago/Turabian StyleNunzio Cennamo; Girolamo D’Agostino; Chiara Perri; Francesco Arcadio; Guido Chiaretti; Eva Parisio; Giulio Camarlinghi; Chiara Vettori; Francesco Di Marzo; Rosario Cennamo; Giovanni Porto; Luigi Zeni. 2021. "Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers." Sensors 21, no. 5: 1681.
A review on bio-chemical label-free sensing applications based on receptors combined with multimode plastic optical fibers (POFs) is carried out. In particular, this work focuses on different kinds of POF intrinsic sensors used in transmission mode, exploiting various types of receptors and useful in several application fields. The sensor structures and the sensing mechanisms of different simple, highly sensitive, and low-cost POF probes are reported and compared. Moreover, different kinds of chemical and biological receptors combined with POF probes, highlighting the advantages and disadvantages of each receptor, are presented in detail. More specifically, the chosen POF sensing platforms, including low-cost and simple interrogation setups, are described showing their ability to monitor specific receptors exploiting intensity variation techniques and plasmonic phenomena, as well. Finally, a prospect of future developments of biochemical sensors with multimode POFs is discussed.
Nunzio Cennamo; Maria Pesavento; Luigi Zeni. A review on simple and highly sensitive plastic optical fiber probes for bio-chemical sensing. Sensors and Actuators B: Chemical 2020, 331, 129393 .
AMA StyleNunzio Cennamo, Maria Pesavento, Luigi Zeni. A review on simple and highly sensitive plastic optical fiber probes for bio-chemical sensing. Sensors and Actuators B: Chemical. 2020; 331 ():129393.
Chicago/Turabian StyleNunzio Cennamo; Maria Pesavento; Luigi Zeni. 2020. "A review on simple and highly sensitive plastic optical fiber probes for bio-chemical sensing." Sensors and Actuators B: Chemical 331, no. : 129393.
A surface plasmon resonance (SPR) platform, based on a D-shaped plastic optical fiber (POF), combined with a biomimetic receptor, i.e., a molecularly imprinted polymer (MIP), is proposed to detect 2-furaldheide (2-FAL) in fermented beverages such as wine. The determination of 2-FAL in food samples is becoming a very crucial task, on the one hand for its role in the flavor and on the other in relation to its toxic and carcinogenic effects on human beings. The proposed sensing device is easy to use and cheap; it has been tested successfully for the detection and quantification of substances of interest in different fields, such as health, the environment and industry. The possibility of performing single-drop measurements is a further favorable aspect for practical applications. As an example, the use of an SPR-MIP sensor for the analysis of 2-FAL in wine, in a concentration range useful for practical applications, is here described.
Maria Pesavento; Nunzio Cennamo; Luigi Zeni; Letizia De Maria; Giancarla Alberti; Daniele Merli. Single Drop Detection of Furfural in Wine by an SPR-Optical Fiber-MIP Based Sensor. Proceedings of The 1st International Electronic Conference on Biosensors 2020, 60, 22 .
AMA StyleMaria Pesavento, Nunzio Cennamo, Luigi Zeni, Letizia De Maria, Giancarla Alberti, Daniele Merli. Single Drop Detection of Furfural in Wine by an SPR-Optical Fiber-MIP Based Sensor. Proceedings of The 1st International Electronic Conference on Biosensors. 2020; 60 (1):22.
Chicago/Turabian StyleMaria Pesavento; Nunzio Cennamo; Luigi Zeni; Letizia De Maria; Giancarla Alberti; Daniele Merli. 2020. "Single Drop Detection of Furfural in Wine by an SPR-Optical Fiber-MIP Based Sensor." Proceedings of The 1st International Electronic Conference on Biosensors 60, no. 1: 22.
We present a novel methodology for magnetic field sensing by exploiting Surface Plasmon Resonance (SPR) sensors based on D-shaped Plastic Optical Fiber (POF) and Magnetic Fluids (MFs). The proposed methodology adopts a multimode POF patch covered with ferrofluid placed between the light source and the SPR-POF platform. The working principle exploits the changing of the SPR spectra due to variations of the SPR resonance conditions generated by the changing in the input light in the SPR-POF sensor due to the attractive force exerted by the target magnetic field on the POF covered with ferrofluid. A model showing a good fitting between the simulated and measured values of the magnetic field is also presented. A proof of concept for this novel sensing approach has been obtained exploiting a prototype of sensor that has been characterized in the range of values between 0.15 mT and 1.2 mT. Particularly, we have estimated in the linear range of the sensor response a sensitivity equal to about 6800 pm/mT and a resolution equal to about 0.029 mT. Moreover, the comparison between the expected and the experimental behaviour has shown a very good match with a mean squared error of about 5%.
Nunzio Cennamo; Francesco Arcadio; Vincenzo Marletta; Salvatore Baglio; Luigi Zeni; Bruno Ando. A Magnetic Field Sensor Based on SPR-POF Platforms and Ferrofluids. IEEE Transactions on Instrumentation and Measurement 2020, 70, 1 -10.
AMA StyleNunzio Cennamo, Francesco Arcadio, Vincenzo Marletta, Salvatore Baglio, Luigi Zeni, Bruno Ando. A Magnetic Field Sensor Based on SPR-POF Platforms and Ferrofluids. IEEE Transactions on Instrumentation and Measurement. 2020; 70 (99):1-10.
Chicago/Turabian StyleNunzio Cennamo; Francesco Arcadio; Vincenzo Marletta; Salvatore Baglio; Luigi Zeni; Bruno Ando. 2020. "A Magnetic Field Sensor Based on SPR-POF Platforms and Ferrofluids." IEEE Transactions on Instrumentation and Measurement 70, no. 99: 1-10.
Over the past three decades, extensive research activity on Brillouin scattering-based distributed optical fiber sensors has led to the availability of commercial instruments capable of measuring the static temperature/strain distribution over kilometer distances and with high spatial resolution, with applications typically covering structural and environmental monitoring. At the same time, the interest in dynamic measurements has rapidly grown due to the relevant number of applications which could benefit from this technology, including structural analysis for defect identification, vibration detection, railway traffic monitoring, shock events detection, and so on. In this paper, we present an overview of the recent advances in Brillouin-based distributed optical fiber sensors for dynamic sensing. The aspects of the Brillouin scattering process relevant in distributed dynamic measurements are analyzed, and the different techniques are compared in terms of performance and hardware complexity.
Agnese Coscetta; Aldo Minardo; Luigi Zeni. Distributed Dynamic Strain Sensing Based on Brillouin Scattering in Optical Fibers. Sensors 2020, 20, 5629 .
AMA StyleAgnese Coscetta, Aldo Minardo, Luigi Zeni. Distributed Dynamic Strain Sensing Based on Brillouin Scattering in Optical Fibers. Sensors. 2020; 20 (19):5629.
Chicago/Turabian StyleAgnese Coscetta; Aldo Minardo; Luigi Zeni. 2020. "Distributed Dynamic Strain Sensing Based on Brillouin Scattering in Optical Fibers." Sensors 20, no. 19: 5629.
Different lines of evidence indicate that monitoring the blood levels of therapeutic antibodies, characterized by high inter-individual variability, can help to optimize clinical decision making, improving patient outcomes and reducing costs with these expensive treatments. A surface plasmon resonance (SPR)-based immunoassay has recently been shown to allow highly reliable and robust monitoring of serum concentrations of infliximab, with significant advantages over classical ELISA. The next level of advancement would be the availability of compact and transportable SPR devices suitable for easy, fast and cheap point-of-care analysis. Here we report the data obtained with recently developed, cost-effective, optical-fibre-based SPR sensors (SPR-POF), which allow the construction of a compact miniaturized system for remote sensing. We carried out an extensive characterization of infliximab binding to an anti-infliximab antibody immobilized on the SPR-POF sensor surface. The present proof-of-principle studies demonstrate the feasibility of the proposed SPR-POF platform for the specific detection of infliximab, in both buffer and human serum, and pave the way for further technological improvements.
Luigi Zeni; Chiara Perri; Nunzio Cennamo; Francesco Arcadio; Girolamo D’Agostino; Mario Salmona; Marten Beeg; Marco Gobbi. A portable optical-fibre-based surface plasmon resonance biosensor for the detection of therapeutic antibodies in human serum. Scientific Reports 2020, 10, 1 -9.
AMA StyleLuigi Zeni, Chiara Perri, Nunzio Cennamo, Francesco Arcadio, Girolamo D’Agostino, Mario Salmona, Marten Beeg, Marco Gobbi. A portable optical-fibre-based surface plasmon resonance biosensor for the detection of therapeutic antibodies in human serum. Scientific Reports. 2020; 10 (1):1-9.
Chicago/Turabian StyleLuigi Zeni; Chiara Perri; Nunzio Cennamo; Francesco Arcadio; Girolamo D’Agostino; Mario Salmona; Marten Beeg; Marco Gobbi. 2020. "A portable optical-fibre-based surface plasmon resonance biosensor for the detection of therapeutic antibodies in human serum." Scientific Reports 10, no. 1: 1-9.
In this work, we have compared several configurations of surface plasmon resonance (SPR) sensors based on D-shaped tapered plastic optical fibers (TPOFs). Particularly, the TPOFs used to obtain the SPR sensors are made by a lab-built system based on two motorized linear positioning stages and a heating plate. Preliminarily, a comparative analysis has been carried out between two different configurations, one with and one without a thin buffer layer deposited between the core of TPOFs and the gold film. After this preliminary step, we have used the simpler configuration, obtained without the buffer layer, to realize different SPR D-shaped TPOF sensors. This study could be of interest in SPR D-shaped multimode plastic optical fiber (POF) sensors because, without the tapers, the performances decrease when the POF’s diameter decreases, whereas the performances improve in SPR D-shaped tapered POF sensors, where the diameter decreases in the D-shaped sensing area. The performances of the SPR sensors based on different taper ratios have been analyzed and compared. The SPR-TPOF sensors have been tested using water–glycerin mixtures with refractive indices ranging from 1.332 to 1.381 RIU. According to the theory, the experimental results have demonstrated that, as the taper ratio increases, the sensitivity of the SPR sensor increases as well, while on the contrary the signal-to-noise ratio (SNR) decreases.
Nunzio Cennamo; Francesco Arcadio; Aldo Minardo; Domenico Montemurro; Luigi Zeni. Experimental Characterization of Plasmonic Sensors Based on Lab-Built Tapered Plastic Optical Fibers. Applied Sciences 2020, 10, 4389 .
AMA StyleNunzio Cennamo, Francesco Arcadio, Aldo Minardo, Domenico Montemurro, Luigi Zeni. Experimental Characterization of Plasmonic Sensors Based on Lab-Built Tapered Plastic Optical Fibers. Applied Sciences. 2020; 10 (12):4389.
Chicago/Turabian StyleNunzio Cennamo; Francesco Arcadio; Aldo Minardo; Domenico Montemurro; Luigi Zeni. 2020. "Experimental Characterization of Plasmonic Sensors Based on Lab-Built Tapered Plastic Optical Fibers." Applied Sciences 10, no. 12: 4389.
The selective detection of pollutants in water in a laboratory scenario has been presented by authors exploiting low-cost optical biosensors based on plastic optical fibers (POFs) and biological or bio-mimetic receptors. For instance, the detection in water of naphthalene, perfluoroalkyl and polyfluoroalkyl substances (PFAs) have been investigated with interesting detection limits when compared to those obtained by using different expensive traditional approaches (e.g. liquid chromatography-mass spectrometry with high performances). In this work, we have developed and tested a novel approach used in a smart measuring system to use POF sensors in situ for the remote measures of pollutants in water for smart cities applications. More specifically, we have used different water-glycerin solutions to test the novel sensor system based on a Raspberry PI connected to the Internet and to a spectrometer, a light source, a POF sensor, and two computers connected to Internet used as client and server.
Nunzio Cennamo; Francesco Arcadio; Fiore Capasso; Chiara Perri; Girolamo D’Agostino; Gianni Porto; Adriano Biasiolo; Luigi Zeni. Towards Smart Selective Sensors exploiting a novel approach to connect Optical Fiber Biosensors in Internet. IEEE Transactions on Instrumentation and Measurement 2020, 69, 1 -1.
AMA StyleNunzio Cennamo, Francesco Arcadio, Fiore Capasso, Chiara Perri, Girolamo D’Agostino, Gianni Porto, Adriano Biasiolo, Luigi Zeni. Towards Smart Selective Sensors exploiting a novel approach to connect Optical Fiber Biosensors in Internet. IEEE Transactions on Instrumentation and Measurement. 2020; 69 (10):1-1.
Chicago/Turabian StyleNunzio Cennamo; Francesco Arcadio; Fiore Capasso; Chiara Perri; Girolamo D’Agostino; Gianni Porto; Adriano Biasiolo; Luigi Zeni. 2020. "Towards Smart Selective Sensors exploiting a novel approach to connect Optical Fiber Biosensors in Internet." IEEE Transactions on Instrumentation and Measurement 69, no. 10: 1-1.
We have used the same bio-mimetic receptor, a specific molecularly imprinted polymer (MIP), on two different plasmonic plastic optical fiber (POF) sensors, to measure how the optical response influences the chemical response. To obtain two surface plasmon resonance (SPR) platforms, we have used two different overlayers between the core of the POF and the gold film. These overlayers, with a refractive index higher than that of the POF’s core (PMMA), have been used to improve the performances of the plasmonic sensor. As overlayers, we have deposited a photoresist (Microposit S1813), in two different ageing conditions, namely before and after its expiry date. In this work, we have demonstrated how the alteration in the photoresist changes the sensor’s performances and eventually the chemical response of the MIPs. More specifically, we have compared the MIPs responses of these two SPR-POF platforms in two different refractive index ranges. For this purpose, an MIP receptor already tested in the detection of Perfluorinated compounds (PFAs) in water, has been exploited.
Nunzio Cennamo; Girolamo DrAgostino; Francesco Arcadio; Chiara Perri; Gianni Porto; Adriano Biasiolo; Luigi Zeni. Measurement of MIPs Responses Deposited on Two SPR-POF Sensors Realized by Different Photoresist Buffer Layers. IEEE Transactions on Instrumentation and Measurement 2020, 69, 1464 -1473.
AMA StyleNunzio Cennamo, Girolamo DrAgostino, Francesco Arcadio, Chiara Perri, Gianni Porto, Adriano Biasiolo, Luigi Zeni. Measurement of MIPs Responses Deposited on Two SPR-POF Sensors Realized by Different Photoresist Buffer Layers. IEEE Transactions on Instrumentation and Measurement. 2020; 69 (4):1464-1473.
Chicago/Turabian StyleNunzio Cennamo; Girolamo DrAgostino; Francesco Arcadio; Chiara Perri; Gianni Porto; Adriano Biasiolo; Luigi Zeni. 2020. "Measurement of MIPs Responses Deposited on Two SPR-POF Sensors Realized by Different Photoresist Buffer Layers." IEEE Transactions on Instrumentation and Measurement 69, no. 4: 1464-1473.
The development of optical biosensors for the rapid and costless determination of clinical biomarkers is of paramount importance in medicine. Here we report a fast and low-cost biosensor based on a plasmonic D-shaped plastic optical fibre (POF) sensor derivatized with an aptamer specific for the recognition of thrombin, the target marker of blood homeostasis and coagulation cascade. In particular, we designed a functional interface based on a Self Assembled Monolayer (SAM) composed of short Poly Ethylene Glycol (PEG) chains and biotin-modified PEG thiol in ratio 8:2 mol:mol, these latter serving as baits for the binding of the aptamer through streptavidin-chemistry. The SAM was studied by X-ray Photoelectron Spectroscopy (XPS) analysis, static contact angle (CA), Surface Plasmon Resonance (SPR) in POFs, and fluorescence microscopy on gold surface. The optimized SAM composition enabled the immobilization of about 112 ng/cm2 of aptamer. The thrombin detection exploiting POF-Aptasensor occurred in short times (5–10 minutes), the reached Limit of Detection (LOD) was about 1 nM, and the detection range was 1.6–60 nM, indicating the POF-Aptasensor well addresses the needs for a low-cost, simple to use and to realize, rapid, small size and portable diagnostic platform.
Nunzio Cennamo; Laura Pasquardini; Francesco Arcadio; Lia E. Vanzetti; Alessandra Maria Bossi; Luigi Zeni. D-shaped plastic optical fibre aptasensor for fast thrombin detection in nanomolar range. Scientific Reports 2019, 9, 1 -9.
AMA StyleNunzio Cennamo, Laura Pasquardini, Francesco Arcadio, Lia E. Vanzetti, Alessandra Maria Bossi, Luigi Zeni. D-shaped plastic optical fibre aptasensor for fast thrombin detection in nanomolar range. Scientific Reports. 2019; 9 (1):1-9.
Chicago/Turabian StyleNunzio Cennamo; Laura Pasquardini; Francesco Arcadio; Lia E. Vanzetti; Alessandra Maria Bossi; Luigi Zeni. 2019. "D-shaped plastic optical fibre aptasensor for fast thrombin detection in nanomolar range." Scientific Reports 9, no. 1: 1-9.
Highly sensitive plasmonic optical fiber platforms combined with receptors have been recently used to obtain selective sensors. A low-cost configuration can be obtained exploiting a D-shaped plastic optical fiber covered with a multilayer sensing surface. The multilayer consists of a gold film, functionalized with a specific receptor, where the surface plasmon resonance (SPR) occurs. The signal is produced by the refractive index variation occurring as a consequence of the receptor-to analyte binding. In this work, a selective sensor for copper(II) detection in drinking water, exploiting a self-assembled monolayer (SAM) of d,l-penicillamine as the sensing layer, has been developed and tested. Different concentrations of copper(II) in NaCl 0.1 M solutions at different pH values and in a real matrix (drinking water) have been considered. The results show that the sensor is able to sense copper(II) at concentrations ranging from 4 × 10-6 M to 2 × 10-4 M. The use of this optical chemical sensor is a very attractive perspective for fast, in situ and low-cost detection of Cu(II) in drinking water for human health concerns. Furthermore, the possibility of remote control is feasible as well, because optical fibers are employed.
Maria Pesavento; Antonella Profumo; Daniele Merli; Lucia Cucca; Luigi Zeni; Nunzio Cennamo. An Optical Fiber Chemical Sensor for the Detection of Copper(II) in Drinking Water. Sensors 2019, 19, 5246 .
AMA StyleMaria Pesavento, Antonella Profumo, Daniele Merli, Lucia Cucca, Luigi Zeni, Nunzio Cennamo. An Optical Fiber Chemical Sensor for the Detection of Copper(II) in Drinking Water. Sensors. 2019; 19 (23):5246.
Chicago/Turabian StyleMaria Pesavento; Antonella Profumo; Daniele Merli; Lucia Cucca; Luigi Zeni; Nunzio Cennamo. 2019. "An Optical Fiber Chemical Sensor for the Detection of Copper(II) in Drinking Water." Sensors 19, no. 23: 5246.