For fruit quality evaluation, sensory characterisation is necessary, to satisfy the needs in both research and along the value chain. Garden strawberry (Fragaria × ananassa Duch.) is globally the most important small fruit, with numerous varieties and intense breeding strategies. In this study, we introduce a multilingual lexicon of 36 sensory attributes, to provide a tool for consistent and comparative sensory evaluations of strawberries across different countries. The attributes were based on scientific literature, selected by a multicultural group of scientists and berry industry actors, and supplied with attribute descriptions in seven languages (English, Finnish, French, German, Italian, Spanish and Turkish). The lexicon was applied by trained sensory panels for validation of attributes related to eating acceptance across three countries, applying the Rate-All-That-Apply (RATA) test, an intensity-based sensory analysis method. We highlight the lexicon’s efficiency and potential to define the fruit quality of different strawberry varieties, and discriminate between them. To identify the most relevant terms in defining the sensory profile of strawberries, the cross-region comparison revealed the importance of the flavours ‘strawberry’, ‘berry’, ‘fruity’, ‘floral’, ‘peach’ and ‘watery’, as well as ‘sweet’ and ‘sour’ tastes, the mouthfeel attributes ‘juicy’, ‘firm’, ‘fibrous’, ‘crunchy’, and the metadescriptor ‘fresh’. Further attributes distinguished between varieties or had local importance. The introduced sensory lexicon can be a key tool for harmonised communication among actors in the strawberry sector, not only sensory professionals, horticultural scientists, and breeders, but also product developers and market stakeholders, despite different cultural backgrounds or native languages, sharing the sensory knowledge of strawberry.