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Bruno Mezzetti; Guy Smagghe; Salvatore Arpaia; Olivier Christiaens; Antje Dietz-Pfeilstetter; Huw Jones; Kaloyan Kostov; Silvia Sabbadini; Hilde-Gunn Opsahl-Sorteberg; Vera Ventura; Clauvis Nji Tizi Taning; Jeremy Sweet. Correction to: RNAi, what is its position in crop protection? Journal of Pest Science 2021, 94, 1555 -1555.
AMA StyleBruno Mezzetti, Guy Smagghe, Salvatore Arpaia, Olivier Christiaens, Antje Dietz-Pfeilstetter, Huw Jones, Kaloyan Kostov, Silvia Sabbadini, Hilde-Gunn Opsahl-Sorteberg, Vera Ventura, Clauvis Nji Tizi Taning, Jeremy Sweet. Correction to: RNAi, what is its position in crop protection? Journal of Pest Science. 2021; 94 (4):1555-1555.
Chicago/Turabian StyleBruno Mezzetti; Guy Smagghe; Salvatore Arpaia; Olivier Christiaens; Antje Dietz-Pfeilstetter; Huw Jones; Kaloyan Kostov; Silvia Sabbadini; Hilde-Gunn Opsahl-Sorteberg; Vera Ventura; Clauvis Nji Tizi Taning; Jeremy Sweet. 2021. "Correction to: RNAi, what is its position in crop protection?" Journal of Pest Science 94, no. 4: 1555-1555.
Editorial on the Research Topic Advances and Challenges of RNAi Based Technologies for Plants In this Research Topic, the focus is on recent research and developments in the use of RNAi techniques to protect plants. Cross kingdom effects of RNA expressed in plants primarily for pathogen control are discussed by Schaefer et al. in Cross-Kingdom RNAi of Pathogen Effectors Leads to Quantitative Adult Plant Resistance in Wheat. Pest as well as pathogen control is considered in Plant miRNA Cross-Kingdom Transfer Targeting Parasitic and Mutualistic Organisms as a Tool to Advance Modern Agriculture by Gualtieri et al.. Bachman et al. consider aspects of their research on dsRNA expressed in maize/corn for root worm beetle control in Sequence–Activity Relationships for the Snf7 Insecticidal dsRNA in Chrysomelidae. The efficiency and efficacy of a system for lepidopteran control is discussed in Comparative Analysis of Chitin SynthaseA dsRNA Mediated RNA Interference for Management of Crop Pests of Different Families of Lepidoptera by Rana et al.. The potential for plants to express RNA viruses which can infect both plants and insects but which are pathogenic in their insect vectors is considered as a strategy for vector insect control in The Use of Engineered Plant Viruses in a Trans-Kingdom Silencing Strategy Against Their Insect Vectors by Kolliopoulou et al.. The biosafety assessments of these and other host induced gene silencing (HIGS) systems are discuss in Biosafety of GM Crop Plants Expressing dsRNA: Data Requirements and EU Regulatory Considerations by Arpaia et al.. Exogenous application of dsRNA (SIGS) for pest and pathogen control is an attractive alternative but is confronted with several problems related to application, uptake, persistence, and efficacy. In Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells, these are discussed by Bennett et al.. Uslu et al. describe studies of high pressure spraying to improve efficacy in High-Pressure-Sprayed Double Stranded RNA Does Not Induce RNA Interference of a Reporter Gene. In relation to virus control, Tabein et al. discuss The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region. Research on control of a major insect pest is reviewed in Validating the Potential of Double-Stranded RNA Targeting Colorado Potato Beetle Mesh Gene in Laboratory and Field Trials by Petek et al.. These papers give an insight into the many areas of research on the potential applications for RNAi for crop protection. Some applications are being commercialized while others are at earlier stages of research (Taning et al., 2020a). The Editors believe that applications of HIGS and SIGS can make major contributions to sustainable and integrated crop protection and support “Green” systems for intensification of agricultural production as advocated in UN Sustainability Development Goals and by FAO, EU, and many national governments (Mezzetti et al., 2020; Taning et al., 2020b). The editors would like to acknowledge the opportunity provided by Frontiers for publishing these papers and thank the authors for their contributions. The editors and many of the authors are members of the iPlanta COST action CA 15223 and further information on iPlanta and publications produced by iPlanta are available at https://iplanta.univpm.it/. All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. This publication is produced by the COST Action iPlanta, supported by COST (European Cooperation in Science and Technology). COST is a funding agency for research and innovation networks. Our Actions help connect research initiatives across Europe and enable scientists to grow their ideas by sharing them with their peers. This boosts their research, career and innovation. Mezzetti, B., Smagghe, G., Arpaia, S., Christiaens, O., Dietz-Pfeilstetter, A., Jones, H., et al. (2020). RNAi: what is its position in agriculture? J. Pest Sci. 93, 1125–1130. doi: 10.1007/s10340-020-01238-2 CrossRef Full Text | Google Scholar Taning, C. N. T., Arpaia, S., Christiaens, O., Dietz-Pfeilstetter, A., Jones, H., Mezzetti, B., et al. (2020a). RNA-based biocontrol compounds: current status and perspectives to reach the market. Pest Manage. Sci. 73, 841–845. doi: 10.1002/ps.5686 PubMed Abstract | CrossRef Full Text | Google Scholar Taning, C. N. T., Mezzetti, B., Kleter, G., Smagghe, G., and Baraldi, E. (2020b). Does RNAi-based technology fit within EU sustainability goals? Trends Biotechnol. 12. doi: 10.1016/j.tibtech.2020.11.008. [Epub ahead of print]. PubMed Abstract | CrossRef Full Text | Google Scholar Keywords: gene silencing (siRNA), cross kingdom, pathogen control, biosafety, sustainable agriculture Citation: Mezzetti B, Fladung M and Sweet J (2021) Editorial: Advances and Challenges of RNAi Based Technologies for Plants. Front. Plant Sci. 12:680242. doi: 10.3389/fpls.2021.680242 Received: 13 March 2021; Accepted: 07 April 2021; Published: 07 May 2021. Edited and reviewed by: Fernando Ponz, Instituto Nacional de Investigación y Tecnología Agroalimentaria (INIA), Spain Copyright © 2021 Mezzetti, Fladung and Sweet. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not...
Bruno Mezzetti; Matthias Fladung; Jeremy Sweet. Editorial: Advances and Challenges of RNAi Based Technologies for Plants. Frontiers in Plant Science 2021, 12, 1 .
AMA StyleBruno Mezzetti, Matthias Fladung, Jeremy Sweet. Editorial: Advances and Challenges of RNAi Based Technologies for Plants. Frontiers in Plant Science. 2021; 12 ():1.
Chicago/Turabian StyleBruno Mezzetti; Matthias Fladung; Jeremy Sweet. 2021. "Editorial: Advances and Challenges of RNAi Based Technologies for Plants." Frontiers in Plant Science 12, no. : 1.
Plant pathogenic fungi are the largest group of disease-causing agents on crop plants and represent a persistent and significant threat to agriculture worldwide. Conventional approaches based on the use of pesticides raise social concern for the impact on the environment and human health and alternative control methods are urgently needed. The rapid improvement and extensive implementation of RNA interference (RNAi) technology for various model and non-model organisms has provided the initial framework to adapt this post-transcriptional gene silencing technology for the management of fungal pathogens. Recent studies showed that the exogenous application of double-stranded RNA (dsRNA) molecules on plants targeting fungal growth and virulence-related genes provided disease attenuation of pathogens like Botrytis cinerea, Sclerotinia sclerotiorum and Fusarium graminearum in different hosts. Such results highlight that the exogenous RNAi holds great potential for RNAi-mediated plant pathogenic fungal disease control. Production of dsRNA can be possible by using either in-vitro or in-vivo synthesis. In this review, we describe exogenous RNAi involved in plant pathogenic fungi and discuss dsRNA production, formulation, and RNAi delivery methods. Potential challenges that are faced while developing a RNAi strategy for fungal pathogens, such as off-target and epigenetic effects, with their possible solutions are also discussed.
Daniel Gebremichael; Zeraye Haile; Francesca Negrini; Silvia Sabbadini; Luca Capriotti; Bruno Mezzetti; Elena Baraldi. RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges. Plants 2021, 10, 650 .
AMA StyleDaniel Gebremichael, Zeraye Haile, Francesca Negrini, Silvia Sabbadini, Luca Capriotti, Bruno Mezzetti, Elena Baraldi. RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges. Plants. 2021; 10 (4):650.
Chicago/Turabian StyleDaniel Gebremichael; Zeraye Haile; Francesca Negrini; Silvia Sabbadini; Luca Capriotti; Bruno Mezzetti; Elena Baraldi. 2021. "RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges." Plants 10, no. 4: 650.
Plant pathogenic fungi are the largest group of disease-causing agents on crop plants and represent a persistent and significant threat to agriculture worldwide. Conventional approaches based on the use of pesticides raise social concern for the impact on the environment and human health and alternative control methods are urgently needed. The rapid improvement and extensive implementation of RNAi technology for various model and non-model organisms has provided the initial framework to adapt this post-transcriptional gene silencing technology for the management of fungal pathogens. In this review, we describe exogenous RNAi involved in plant pathogenic fungi and discuss small RNA production, formulation, and RNAi delivery methods. We explore some challenges with possible solutions. Furthermore, exogenous RNAi holds great potential for RNAi-mediated plant pathogenic fungal disease control.
Daniel Gebremichael; Zeraye Mehari Haile; Francesca Negrini; Silvia Sabbadini; Luca Capriotti; Bruno Mezzetti; Elena Baraldi. RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges. 2021, 1 .
AMA StyleDaniel Gebremichael, Zeraye Mehari Haile, Francesca Negrini, Silvia Sabbadini, Luca Capriotti, Bruno Mezzetti, Elena Baraldi. RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges. . 2021; ():1.
Chicago/Turabian StyleDaniel Gebremichael; Zeraye Mehari Haile; Francesca Negrini; Silvia Sabbadini; Luca Capriotti; Bruno Mezzetti; Elena Baraldi. 2021. "RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges." , no. : 1.
Recently, the consumption of healthy foods has been related to the prevention of cardiovascular, degenerative diseases and different forms of cancers, underlying the importance of the diet for the consumer’s health. Fruits and vegetables contain phytochemicals that act as protective factors for the human body, through different mechanisms of action. Among vegetables, Brassica received a lot of attention in the last years for the phytochemical compounds content and antioxidant capacity that confer nutraceutical value to the product. The amount of healthy bioactive compounds present in the Brassica defines the nutritional quality. These molecules could belong to the class of antioxidant compounds (e.g., phenols, vitamin C, etc.), or to non-antioxidant compounds (e.g., minerals, glucosinolates, etc.). The amount of these compounds in Brassica vegetables could be influenced by several factors, depending on the genotypes, the environmental conditions and the cultivation techniques adopted. The aim of this study is to highlight the main phytochemical compounds present in brassicas used as a food vegetable that confer nutritional and sensorial quality to the final product, and to investigate the main factors that affect the phytochemical concentration and the overall quality of Brassica vegetables.
Francesca Biondi; Francesca Balducci; Franco Capocasa; Marino Visciglio; Elena Mei; Massimo Vagnoni; Bruno Mezzetti; Luca Mazzoni. Environmental Conditions and Agronomical Factors Influencing the Levels of Phytochemicals in Brassica Vegetables Responsible for Nutritional and Sensorial Properties. Applied Sciences 2021, 11, 1927 .
AMA StyleFrancesca Biondi, Francesca Balducci, Franco Capocasa, Marino Visciglio, Elena Mei, Massimo Vagnoni, Bruno Mezzetti, Luca Mazzoni. Environmental Conditions and Agronomical Factors Influencing the Levels of Phytochemicals in Brassica Vegetables Responsible for Nutritional and Sensorial Properties. Applied Sciences. 2021; 11 (4):1927.
Chicago/Turabian StyleFrancesca Biondi; Francesca Balducci; Franco Capocasa; Marino Visciglio; Elena Mei; Massimo Vagnoni; Bruno Mezzetti; Luca Mazzoni. 2021. "Environmental Conditions and Agronomical Factors Influencing the Levels of Phytochemicals in Brassica Vegetables Responsible for Nutritional and Sensorial Properties." Applied Sciences 11, no. 4: 1927.
The increasing evidences on the relation between diet and human health are driving the consumers toward the choice of foods with high amount of nutrients, considered the molecules responsible for the regulation of several metabolic and biochemical processes, and for the prevention of many chronic diseases. Among food products, fruits represent a natural source of many different nutrients and possess much importance in a balanced diet. The increase of the micro/macronutrient levels and the decrease of antinutrients amount are considered main objectives for the improvement of fruit trees nutritional value. This review summarizes many of the attempts made to increase the nutritional quality of fruit tree species during the last decades through the application of traditional and molecular breeding technologies, and the most recent New breeding techniques (NBTs). New genotypes with improved nutritional quality have been obtained for different fruit tree species, either by increasing the content of well-known beneficial molecules, such as phenolic compounds, vitamins, and carotenoids, or by decreasing the levels of specific antinutrients. The development of molecular techniques and biotechnological tools allowed the identification and validation of candidate genes involved in the regulation of specific classes of nutritional compounds in fruit trees, and useful to obtain nutritionally improved products. NBTs represent alternative tools to classical breeding techniques, to regulate one or more fruit trees key nutrients in a more quick and precise manner. However, genetic engineering approaches still present public and regulatory concerns that strongly limit their exploitation.
Silvia Sabbadini; Franco Capocasa; Maurizio Battino; Luca Mazzoni; Bruno Mezzetti. Improved nutritional quality in fruit tree species through traditional and biotechnological approaches. Trends in Food Science & Technology 2021, 1 .
AMA StyleSilvia Sabbadini, Franco Capocasa, Maurizio Battino, Luca Mazzoni, Bruno Mezzetti. Improved nutritional quality in fruit tree species through traditional and biotechnological approaches. Trends in Food Science & Technology. 2021; ():1.
Chicago/Turabian StyleSilvia Sabbadini; Franco Capocasa; Maurizio Battino; Luca Mazzoni; Bruno Mezzetti. 2021. "Improved nutritional quality in fruit tree species through traditional and biotechnological approaches." Trends in Food Science & Technology , no. : 1.
Plant-derived exosome-like nanovesicles (EPDENs) have recently been isolated and evaluated as potential bioactive nutraceutical biomolecules. It has been hypothesized that EPDENs may exert their activity on mammalian cells through their specific cargo. In this study, we isolated and purified EPDENs from the strawberry juice of Fragaria x ananassa (cv. Romina), a new cultivar characterized by a high content of anthocyanins, folic acid, flavonols, and vitamin C and an elevated antioxidant capacity. Fragaria-derived EPDENs were purified by a series of centrifugation and filtration steps. EPDENs showed size and morphology similar to mammalian extracellular nanovesicles. The internalization of Fragaria-derived EPDENs by human mesenchymal stromal cells (MSCs) did not negatively affect their viability, and the pretreatment of MSCs with Fragaria-derived EPDENs prevented oxidative stress in a dose-dependent manner. This is possibly due to the presence of vitamin C inside the nanovesicle membrane. The analysis of EPDEN cargo also revealed the presence of small RNAs and miRNAs. These findings suggest that Fragaria-derived EPDENs may be considered nanoshuttles contained in food, with potential health-promoting activity.
Francesca Perut; Laura Roncuzzi; Sofia Avnet; Annamaria Massa; Nicoletta Zini; Silvia Sabbadini; Francesca Giampieri; Bruno Mezzetti; Nicola Baldini. Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells. Biomolecules 2021, 11, 87 .
AMA StyleFrancesca Perut, Laura Roncuzzi, Sofia Avnet, Annamaria Massa, Nicoletta Zini, Silvia Sabbadini, Francesca Giampieri, Bruno Mezzetti, Nicola Baldini. Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells. Biomolecules. 2021; 11 (1):87.
Chicago/Turabian StyleFrancesca Perut; Laura Roncuzzi; Sofia Avnet; Annamaria Massa; Nicoletta Zini; Silvia Sabbadini; Francesca Giampieri; Bruno Mezzetti; Nicola Baldini. 2021. "Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells." Biomolecules 11, no. 1: 87.
An increasing number of epidemiological studies highlights a remarkable association between a diet rich in fruits and vegetables and a lower incidence of different inflammatory-related pathologies. Berries represent an interesting source of phytochemicals and nutrients, widely investigated for their role in health promotion and disease prevention. the aim of this review was to summarize and update the effect of different berry extracts, their fractions and single bioactive compounds against the inflammatory status promoted by the Gram-negative bacteria endotoxin lipopolysaccharide (LPS). The main molecular mechanisms involved have been elucidated, focusing particular attention on the biological response evoked in different in vitro and in vivo models. the inhibition of inflammatory response mediated by MAPK and NF-kB is the main molecular pathway involved in berries anti-inflammatory role, especially in grape and blueberry which represent the main investigated fruits, improving antioxidant defence and exerting beneficial effects in the maintenance of healthy conditions in LPS-treated models.
Massimiliano Gasparrini; Tamara Y. Forbes-Hernandez; Danila Cianciosi; José L. Quiles; Bruno Mezzetti; Jianbo Xiao; Francesca Giampieri; Maurizio Battino. The efficacy of berries against lipopolysaccharide-induced inflammation: A review. Trends in Food Science & Technology 2021, 1 .
AMA StyleMassimiliano Gasparrini, Tamara Y. Forbes-Hernandez, Danila Cianciosi, José L. Quiles, Bruno Mezzetti, Jianbo Xiao, Francesca Giampieri, Maurizio Battino. The efficacy of berries against lipopolysaccharide-induced inflammation: A review. Trends in Food Science & Technology. 2021; ():1.
Chicago/Turabian StyleMassimiliano Gasparrini; Tamara Y. Forbes-Hernandez; Danila Cianciosi; José L. Quiles; Bruno Mezzetti; Jianbo Xiao; Francesca Giampieri; Maurizio Battino. 2021. "The efficacy of berries against lipopolysaccharide-induced inflammation: A review." Trends in Food Science & Technology , no. : 1.
European Union (EU) and global sustainability policies emphasize the need to replace contentious pesticides with safe, efficient, and cost-effective alternatives to ensure sustainable food production. However, R&D for alternatives to contentious pesticides are lagging behind and need to be broadened. Here, we discuss how RNAi-based technology can contribute to pesticide risk reduction.
Clauvis Nji Tizi Taning; Bruno Mezzetti; Gijs Kleter; Guy Smagghe; Elena Baraldi. Does RNAi-Based Technology Fit within EU Sustainability Goals? Trends in Biotechnology 2020, 39, 644 -647.
AMA StyleClauvis Nji Tizi Taning, Bruno Mezzetti, Gijs Kleter, Guy Smagghe, Elena Baraldi. Does RNAi-Based Technology Fit within EU Sustainability Goals? Trends in Biotechnology. 2020; 39 (7):644-647.
Chicago/Turabian StyleClauvis Nji Tizi Taning; Bruno Mezzetti; Gijs Kleter; Guy Smagghe; Elena Baraldi. 2020. "Does RNAi-Based Technology Fit within EU Sustainability Goals?" Trends in Biotechnology 39, no. 7: 644-647.
Downy mildew, powdery mildew, and grey mold are some of the phytopathological diseases causing economic losses in agricultural crops, including grapevine, worldwide. In the current scenario of increasing global warming, in which the massive use of agrochemicals should be limited, the management of fungal disease has become a challenge. The knowledge acquired on candidate resistant (R) genes having an active role in plant defense mechanisms has allowed numerous breeding programs to integrate these traits into selected cultivars, even though with some limits in the conservation of the proper qualitative characteristics of the original clones. Given their gene-specific mode of action, biotechnological techniques come to the aid of breeders, allowing them to generate simple and fast modifications in the host, without introducing other undesired genes. The availability of efficient gene transfer procedures in grapevine genotypes provide valid tools that support the application of new breeding techniques (NBTs). The expertise built up over the years has allowed the optimization of these techniques to overexpress genes that directly or indirectly limit fungal and oomycetes pathogens growth or silence plant susceptibility genes. Furthermore, the downregulation of pathogen genes which act as virulence effectors by exploiting the RNA interference mechanism, represents another biotechnological tool that increases plant defense. In this review, we summarize the most recent biotechnological strategies optimized and applied on Vitis species, aimed at reducing their susceptibility to the most harmful fungal and oomycetes diseases. The best strategy for combating pathogenic organisms is to exploit a holistic approach that fully integrates all these available tools.
Luca Capriotti; Elena Baraldi; Bruno Mezzetti; Cecilia Limera; Silvia Sabbadini. Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine. International Journal of Molecular Sciences 2020, 21, 5701 .
AMA StyleLuca Capriotti, Elena Baraldi, Bruno Mezzetti, Cecilia Limera, Silvia Sabbadini. Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine. International Journal of Molecular Sciences. 2020; 21 (16):5701.
Chicago/Turabian StyleLuca Capriotti; Elena Baraldi; Bruno Mezzetti; Cecilia Limera; Silvia Sabbadini. 2020. "Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine." International Journal of Molecular Sciences 21, no. 16: 5701.
Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established. Many strategies have been tested in order to obtain an efficient peach transformation system. Despite the amount of time and the efforts invested, the lack of success has significantly limited the utility of peach as a model genetic system for trees, despite its relatively short generation time; small, high-quality genome; and well-studied genetic resources. Additionally, the absence of efficient genetic transformation protocols precludes the application of many biotechnological tools in peach breeding programs. In this review, we provide an overview of research on regeneration and genetic transformation in this species and summarize novel strategies and procedures aimed at producing transgenic peaches. Promising future approaches to develop a robust peach transformation system are discussed, focusing on the main bottlenecks to success including the low efficiency of A. tumefaciens-mediated transformation, the low level of correspondence between cells competent for transformation and those that have regenerative competence, and the high rate of chimerism in the few shoots that are produced following transformation.
Angela Ricci; Silvia Sabbadini; Humberto Prieto; Isabel Mg Padilla; Chris Dardick; Zhijian Li; Ralph Scorza; Cecilia Limera; Bruno Mezzetti; Margarita Perez-Jimenez; Lorenzo Burgos; Cesar Petri. Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward. Plants 2020, 9, 971 .
AMA StyleAngela Ricci, Silvia Sabbadini, Humberto Prieto, Isabel Mg Padilla, Chris Dardick, Zhijian Li, Ralph Scorza, Cecilia Limera, Bruno Mezzetti, Margarita Perez-Jimenez, Lorenzo Burgos, Cesar Petri. Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward. Plants. 2020; 9 (8):971.
Chicago/Turabian StyleAngela Ricci; Silvia Sabbadini; Humberto Prieto; Isabel Mg Padilla; Chris Dardick; Zhijian Li; Ralph Scorza; Cecilia Limera; Bruno Mezzetti; Margarita Perez-Jimenez; Lorenzo Burgos; Cesar Petri. 2020. "Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward." Plants 9, no. 8: 971.
In the present study, an efficient system for the in vitro regeneration of adventitious shoots from the peach rootstock Hansen 536 leaves has been established. Twenty regeneration media containing McCown Woody Plant Medium (WPM) as a basal salt supplemented with different concentrations and combinations of plant growth regulators (PGRs) were tested. Expanded leaves along with their petiole from 3-week-old elongated in vitro shoot cultures were used as starting explants. The highest regeneration rate (up to 53%) was obtained on WPM basal medium enriched with 15.5 μM N6-benzylaminopurine (BAP). The influences on leaf regeneration of the ethylene inhibitor silver thiosulphate (STS) and of different combinations of antibiotics added to the optimized regeneration medium were also investigated. The use of 10 μM STS or carbenicillin (238 mM) combined with cefotaxime (210 μM) significantly increased the average number of regenerating shoots per leaf compared to the control. In vitro shoots were finally elongated, rooted and successfully acclimatized in the greenhouse. The results achieved in this study advances the knowledge on factors affecting leaf organogenesis in Prunus spp., and the regeneration protocol described looks promising for the optimization of new genetic transformation procedures in Hansen 536 and other peach rootstocks and cultivars.
Angela Ricci; Luca Capriotti; Bruno Mezzetti; Oriano Navacchi; Silvia Sabbadini. Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536. Plants 2020, 9, 1 .
AMA StyleAngela Ricci, Luca Capriotti, Bruno Mezzetti, Oriano Navacchi, Silvia Sabbadini. Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536. Plants. 2020; 9 (6):1.
Chicago/Turabian StyleAngela Ricci; Luca Capriotti; Bruno Mezzetti; Oriano Navacchi; Silvia Sabbadini. 2020. "Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536." Plants 9, no. 6: 1.
RNA interference (RNAi) is being developed and exploited to improve plants by modifying endogenous gene expression as well as to target pest and pathogen genes both within plants (i.e. host-induced gene silencing) and/or as topical applications (e.g. spray-induced gene silencing). RNAi is a natural mechanism which can be exploited to make a major contribution towards integrated pest management and sustainable agricultural strategies needed worldwide to secure current and future food production. RNAi plants are being assessed and regulated using existing regulatory frameworks for GMO. However, there is an urgent need to develop appropriate science-based risk assessment procedures for topical RNAi applications within existing plant protection products legislation.
Bruno Mezzetti; Guy Smagghe; Salvatore Arpaia; Olivier Christiaens; Antje Dietz-Pfeilstetter; Huw Jones; Kaloyan Kostov; Silvia Sabbadini; Hilde-Gunn Opsahl-Sorteberg; Vera Ventura; Clauvis Nji Tizi Taning; Jeremy Sweet. RNAi: What is its position in agriculture? Journal of Pest Science 2020, 93, 1125 -1130.
AMA StyleBruno Mezzetti, Guy Smagghe, Salvatore Arpaia, Olivier Christiaens, Antje Dietz-Pfeilstetter, Huw Jones, Kaloyan Kostov, Silvia Sabbadini, Hilde-Gunn Opsahl-Sorteberg, Vera Ventura, Clauvis Nji Tizi Taning, Jeremy Sweet. RNAi: What is its position in agriculture? Journal of Pest Science. 2020; 93 (4):1125-1130.
Chicago/Turabian StyleBruno Mezzetti; Guy Smagghe; Salvatore Arpaia; Olivier Christiaens; Antje Dietz-Pfeilstetter; Huw Jones; Kaloyan Kostov; Silvia Sabbadini; Hilde-Gunn Opsahl-Sorteberg; Vera Ventura; Clauvis Nji Tizi Taning; Jeremy Sweet. 2020. "RNAi: What is its position in agriculture?" Journal of Pest Science 93, no. 4: 1125-1130.
The determination of strawberry fruit quality through the traditional destructive lab techniques has some limitations related to the amplitude of the samples, the timing and the applicability along all phases of the supply chain. The aim of this study was to determine the main qualitative characteristics through traditional lab destructive techniques and Near Infrared Spectroscopy (NIR) in fruits of five strawberry genotypes. Principal Component Analysis (PCA) was applied to search for spectral differences among all the collected samples. A Partial Least Squares regression (PLS) technique was computed in order to predict the quality parameters of interest. The PLS model for the soluble solids content prediction was the best performing—in fact, it is a robust and reliable model and the validation values suggested possibilities for its use in quality applications. A suitable PLS model is also obtained for the firmness prediction—the validation values tend to worsen slightly but can still be accepted in screening applications. NIR spectroscopy represents an important alternative to destructive techniques, using the infrared region of the electromagnetic spectrum to investigate in a non-destructive way the chemical–physical properties of the samples, finding remarkable applications in the agro-food market.
Manuela Mancini; Luca Mazzoni; Francesco Gagliardi; Francesca Balducci; Daniele Duca; Giuseppe Toscano; Bruno Mezzetti; Franco Capocasa. Application of the Non-Destructive NIR Technique for the Evaluation of Strawberry Fruits Quality Parameters. Foods 2020, 9, 441 .
AMA StyleManuela Mancini, Luca Mazzoni, Francesco Gagliardi, Francesca Balducci, Daniele Duca, Giuseppe Toscano, Bruno Mezzetti, Franco Capocasa. Application of the Non-Destructive NIR Technique for the Evaluation of Strawberry Fruits Quality Parameters. Foods. 2020; 9 (4):441.
Chicago/Turabian StyleManuela Mancini; Luca Mazzoni; Francesco Gagliardi; Francesca Balducci; Daniele Duca; Giuseppe Toscano; Bruno Mezzetti; Franco Capocasa. 2020. "Application of the Non-Destructive NIR Technique for the Evaluation of Strawberry Fruits Quality Parameters." Foods 9, no. 4: 441.
Background Cultivation of highbush blueberry (Vaccinium corymbosum L.) is increasing in Europe in the last years, in particular due to the availability of new genotypes suitable for the cultivation in many different environmental conditions. The aim of this study was to evaluate the resilience and nutritional quality of eleven highbush blueberry cultivars and two new selections (from The New Zealand Institute for Plant & Food Research Ltd breeding program) to Mediterranean hot summer climate conditions, by measuring: plant yield; seasonality; fruit sensorial traits; phytochemical content in fruits. Results The new blueberry genotype PFR005 showed high adaptability to these environmental conditions, with the highest total plant yield, while PFR075 was the best genotypes for the nutritional characteristics. Among cultivars, ‘Cosmopolitan’ showed the maximum average fruit weight, ‘Blueray’ and ‘Hortblue Poppins’ demonstrated a good sensorial profile, while the best cultivars from the nutritional point of view were ‘Hortblue Poppins’, ‘Hortblue Petite’ and ‘Early Blue’. Conclusion Cultivated varieties or new genotypes could be suitable to satisfy the needs of different actors of the productive chain. The integration of the germplasm evaluation with tailored breeding program will help in the next future to create new cultivars useful to expand blueberry cultivation in Mediterranean hot summer climate conditions, up to now of high limitation for this crop. This article is protected by copyright. All rights reserved.
Luca Mazzoni; Francesca Balducci; Lucia Di Vittori; Jessica Scalzo; Franco Capocasa; Chuan-Fei Zhong; Tamara Y. Forbes-Hernandez; Francesca Giampieri; Maurizio Battino; Bruno Mezzetti. Yield and nutritional quality of highbush blueberry genotypes trialled in a Mediterranean hot summer climate. Journal of the Science of Food and Agriculture 2020, 100, 3675 -3686.
AMA StyleLuca Mazzoni, Francesca Balducci, Lucia Di Vittori, Jessica Scalzo, Franco Capocasa, Chuan-Fei Zhong, Tamara Y. Forbes-Hernandez, Francesca Giampieri, Maurizio Battino, Bruno Mezzetti. Yield and nutritional quality of highbush blueberry genotypes trialled in a Mediterranean hot summer climate. Journal of the Science of Food and Agriculture. 2020; 100 (9):3675-3686.
Chicago/Turabian StyleLuca Mazzoni; Francesca Balducci; Lucia Di Vittori; Jessica Scalzo; Franco Capocasa; Chuan-Fei Zhong; Tamara Y. Forbes-Hernandez; Francesca Giampieri; Maurizio Battino; Bruno Mezzetti. 2020. "Yield and nutritional quality of highbush blueberry genotypes trialled in a Mediterranean hot summer climate." Journal of the Science of Food and Agriculture 100, no. 9: 3675-3686.
The strawberry breeding is now aimed to produce new cultivars combining high plant adaptability and yield with high sensorial and nutritional quality of the fruit. The aim of this study is to assess the breeding progress achieved in 3 backcross generations (BC1, BC2, BC3) from F1 Fragaria x ananassa (Fxa) x F. virginiana glauca (FVG) interspecific crosses, in terms of productive parameters (plant commercial yield) and the sensorial quality (Average Fruit Weight, Soluble Solids and Titratable Acidity). Among those genotypes, the most interesting were selected and analyzed for nutritional parameters (Total Antioxidant Capacity, Total Phenolics Content and Total Anthocyanins Content by spectrophotometer; Vitamin C, specific Anthocyanins and specific Phenolic Acids through HPLC). For the sensorial analyses, the BC3 genotypes showed higher Soluble Solids content in respect to BC1 and BC2. Regarding the Titratable Acidity, F1 showed the highest values. Also for the qualitative parameters, BC3 showed a higher value, in particular for the Total Antioxidant Capacity and phenolic acids content. For the Total Phenolics Content and the Total Anthocyanins Content the highest values were registered in F1 and Fxa (aimed to Industry), respectively. Finally, in the case of Vitamin C, the BC1 had fruits rich in this compound. The highest values for anthocyanins content were registered in Fxa (aimed to Industry). The results of the back-crossing program developed at UNIVPM-D3A showed that the BC3 generation possess many improved characters, in respect to Fxa genotype and the F1 generation.
Luca Mazzoni; Lucia Di Vittori; Francesca Balducci; Tamara Y. Forbes-Hernández; Francesca Giampieri; Maurizio Battino; Bruno Mezzetti; Franco Capocasa. Sensorial and nutritional quality of inter and intra—Specific strawberry genotypes selected in resilient conditions. Scientia Horticulturae 2019, 261, 108945 .
AMA StyleLuca Mazzoni, Lucia Di Vittori, Francesca Balducci, Tamara Y. Forbes-Hernández, Francesca Giampieri, Maurizio Battino, Bruno Mezzetti, Franco Capocasa. Sensorial and nutritional quality of inter and intra—Specific strawberry genotypes selected in resilient conditions. Scientia Horticulturae. 2019; 261 ():108945.
Chicago/Turabian StyleLuca Mazzoni; Lucia Di Vittori; Francesca Balducci; Tamara Y. Forbes-Hernández; Francesca Giampieri; Maurizio Battino; Bruno Mezzetti; Franco Capocasa. 2019. "Sensorial and nutritional quality of inter and intra—Specific strawberry genotypes selected in resilient conditions." Scientia Horticulturae 261, no. : 108945.
Facing current climate challenges and drastically reduced chemical options for plant protection, the exploitation of RNA interference (RNAi) as an agricultural biotechnology tool has unveiled possible new solutions to the global problems of agricultural losses caused by pests and other biotic and abiotic stresses. While the use of RNAi as a tool in agriculture is still limited to a few transgenic crops, and only adopted in restricted parts of the world, scientists and industry are already seeking innovations in leveraging and exploiting the potential of RNAi in the form of RNA‐based biocontrol compounds for external applications. Here, we highlight the expanding research and development pipeline, commercial landscape and regulatory environment surrounding the pursuit of RNA‐based biocontrol compounds with improved environmental profiles. The commitments of well‐established agrochemical companies to invest in research endeavours and the role of start‐up companies are crucial for the successful development of practical applications for these compounds. Additionally, the availability of standardized guidelines to tackle regulatory ambiguities surrounding RNA‐based biocontrol compounds will help to facilitate the entire commercialization process. Finally, communication to create awareness and public acceptance will be key to the deployment of these compounds. This article is protected by copyright. All rights reserved.
Clauvis Nji Tizi Taning; Salvatore Arpaia; Olivier Christiaens; Antje Dietz‐Pfeilstetter; Huw Jones; Bruno Mezzetti; Silvia Sabbadini; Hilde‐Gunn Sorteberg; Jeremy Sweet; Vera Ventura; Guy Smagghe. RNA‐based biocontrol compounds: current status and perspectives to reach the market. Pest Management Science 2019, 76, 841 -845.
AMA StyleClauvis Nji Tizi Taning, Salvatore Arpaia, Olivier Christiaens, Antje Dietz‐Pfeilstetter, Huw Jones, Bruno Mezzetti, Silvia Sabbadini, Hilde‐Gunn Sorteberg, Jeremy Sweet, Vera Ventura, Guy Smagghe. RNA‐based biocontrol compounds: current status and perspectives to reach the market. Pest Management Science. 2019; 76 (3):841-845.
Chicago/Turabian StyleClauvis Nji Tizi Taning; Salvatore Arpaia; Olivier Christiaens; Antje Dietz‐Pfeilstetter; Huw Jones; Bruno Mezzetti; Silvia Sabbadini; Hilde‐Gunn Sorteberg; Jeremy Sweet; Vera Ventura; Guy Smagghe. 2019. "RNA‐based biocontrol compounds: current status and perspectives to reach the market." Pest Management Science 76, no. 3: 841-845.
Francesca Balducci; Luca Capriotti; Luca Mazzoni; Irene Medori; Andrea Albanesi; Francesca Giampieri; Bruno Mezzetti; Franco Capocasa. The rootstock effects on vigor, production and fruit quality in sweet cherry (Prunus avium L.). Journal of Berry Research 2019, 9, 249 -265.
AMA StyleFrancesca Balducci, Luca Capriotti, Luca Mazzoni, Irene Medori, Andrea Albanesi, Francesca Giampieri, Bruno Mezzetti, Franco Capocasa. The rootstock effects on vigor, production and fruit quality in sweet cherry (Prunus avium L.). Journal of Berry Research. 2019; 9 (2):249-265.
Chicago/Turabian StyleFrancesca Balducci; Luca Capriotti; Luca Mazzoni; Irene Medori; Andrea Albanesi; Francesca Giampieri; Bruno Mezzetti; Franco Capocasa. 2019. "The rootstock effects on vigor, production and fruit quality in sweet cherry (Prunus avium L.)." Journal of Berry Research 9, no. 2: 249-265.
Prunus spp. is one of the most recalcitrant fruit tree species in terms of in vitro regeneration and transformation, mostly when mature tissues are used as explants. The present study describes the in vitro regeneration via indirect organogenesis, and Agrobacterium tumefaciens-mediated transformation of the peach rootstock Hansen 536 (Prunus persica × Prunus amygdalus) through the use of meristematic bulks (MBs) as starting explants. Efficient adventitious shoot regeneration was obtained when Hansen 536 MBs were cultured on an optimized medium consisting of modified McCown Woody Plant medium (WPM) enriched with 4.4 M 6-Benzyladenine (BA), 0.1 M 1-Naphthaleneacetic acid (NAA) and 6.0 g L−1 plant agar S1000 (B&V). MB slices were used later as starting explants for Agrobacterium-mediated transformation to introduce an RNAi construct “ihp35S-PPV194” against PPV virus. Transgenic events were identified by both green fluorescent protein (GFP) screening and kanamycin selection at different concentrations (0, 17 or 42 M). GFP-fluorescent proliferating callus lines were selected and confirmed to stably express the ihp35S-PPV194::eGFP gene construct by molecular analysis. Although shoot regeneration from these transgenic calli has not been obtained yet, this represents one of the few examples of successful attempts in peach genetic transformation from somatic tissues, and also serves as a useful in vitro system for future gene functional analysis in peach.
Silvia Sabbadini; Angela Ricci; Cecilia Limera; Dania Baldoni; Luca Capriotti; Bruno Mezzetti. Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus. Plants 2019, 8, 178 .
AMA StyleSilvia Sabbadini, Angela Ricci, Cecilia Limera, Dania Baldoni, Luca Capriotti, Bruno Mezzetti. Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus. Plants. 2019; 8 (6):178.
Chicago/Turabian StyleSilvia Sabbadini; Angela Ricci; Cecilia Limera; Dania Baldoni; Luca Capriotti; Bruno Mezzetti. 2019. "Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus." Plants 8, no. 6: 178.
The success of in vitro plant regeneration and the competence of genetic transformation greatly depends on the genotype of the species of interest. In previous work, we developed a method for the efficient Agrobacterium-mediated genetic transformation via organogenesis of V. vinifera cultivar Thompson Seedless, by using meristematic bulk (MB) as starting tissue. In this study, we applied this method for the regeneration and transformation of MBs obtained from the Italian cultivar Ciliegiolo and two of the commonly used Vitis rootstocks, 110 Richter and Kober 5BB, in comparison with Thompson Seedless. The A. tumefaciens strain EHA105, harbouring pK7WG2 binary vector, was used for the transformation trials, which allowed selection through the enhanced-green fluorescent protein (eGFP) and the neomycin phosphotransferase (nptII) gene. Putative transformed tissues and/or shoots were identified by either a screening based on the eGFP expression alone or its use in combination with kanamycin in the medium. MBs obtained from Thompson Seedless showed the highest regeneration and transformation cell competence, which subsequently allowed the recovery of stably transformed plants. Ciliegiolo, 110 Richter, and Kober 5BB, produced actively growing transgenic calli showing eGFP fluorescence, more consistently on selective media, but had no regenerative competence.
S. Sabbadini; L. Capriotti; B. Molesini; T. Pandolfini; O. Navacchi; C. Limera; A. Ricci; B. Mezzetti. Comparison of regeneration capacity and Agrobacterium-mediated cell transformation efficiency of different cultivars and rootstocks of Vitis spp. via organogenesis. Scientific Reports 2019, 9, 582 .
AMA StyleS. Sabbadini, L. Capriotti, B. Molesini, T. Pandolfini, O. Navacchi, C. Limera, A. Ricci, B. Mezzetti. Comparison of regeneration capacity and Agrobacterium-mediated cell transformation efficiency of different cultivars and rootstocks of Vitis spp. via organogenesis. Scientific Reports. 2019; 9 (1):582.
Chicago/Turabian StyleS. Sabbadini; L. Capriotti; B. Molesini; T. Pandolfini; O. Navacchi; C. Limera; A. Ricci; B. Mezzetti. 2019. "Comparison of regeneration capacity and Agrobacterium-mediated cell transformation efficiency of different cultivars and rootstocks of Vitis spp. via organogenesis." Scientific Reports 9, no. 1: 582.