In this review, the composition, characteristics, and management of dairy buffalo calves were examined and compared with bovines. The neonatal period is critical for buffalo calves and is characterized by a high mortality rate (more than 40%). The early intake of high-quality colostrum (IgG > 50 mg/mL) is the one way to improve the immune system of calves (serum IgG > 10 mg/mL after 12 h), thus increasing their chances of survival. Mainly in intensive farms, the availability of high-quality colostrum is necessary; thus, good quality colostrum is often stored to provide newborn calves which cannot be fed by their mothers. Also, the manipulation of the immunological status of animals through vaccination has been depicted since the quality of colostrum tended to be influenced by vaccination against pathogens. Buffalo breeding is constantly expanding in Italy, mainly thanks to the Mozzarella cheese production that represents the excellence of the “Made in Italy” and is exported worldwide. Indeed, high calf mortality rates directly affect the profitability of the business. For these reasons, the aim of this review was to examine specific research on buffalo colostrum that, compared with other species, are scarce. Improving the knowledge of buffalo colostrum, in terms of characteristics and management, is critical to guarantee buffalo newborns’ health in order to reduce their mortality rate. Importantly, considering the knowledge on cattle valid also for buffalo is a widespread, and often erroneous, habit in several fields, including colostrum feeding. Therefore, the two species were compared in this review.

In formulating balanced rations for ruminants, knowing the chemical composition of forage and feeds in general is crucial to create adequate nutritional plans that meet animals’ feed requirements. From July to December of 2020, a total of 175 samples of corn silage hybrid PR31Y43 grown (135 days of maturity class) in three sites (Piana del Sele, Vallo di Diano and Caserta) of the Campania region (South Italy) were collected and analysed by using a portable Near Infrared Spectroscopy (NIRS). The area of cultivation/storage of corn silage highly (p < 0.05) affected the nutritional characteristics of the analysed samples. The silages produced in the Vallo di Diano showed the significantly highest (p < 0.05) DM content compared to those from the other sites (349.3 vs. 323.4 and 328.1 g/kg as feed from Caserta and Piana del Sele, respectively). The structural carbohydrates were significantly higher (p < 0.05) in Piana del Sele feed than those in both Caserta and Vallo di Diano feeds (420.1 vs. 396.7 and 397.6 g/kg as feed), whereas the non-fibrous carbohydrates were significantly higher in Caserta and Vallo di Diano feeds (p < 0.05; 469.6 and 471.8 g/kg as feed); intermediate values were registered in the corn silages produced in Piana del Sele (446.6 g/kg as feed). No differences were detected for protein levels. The NIRS technology could be useful to obtain fast and accurate picture of silage quality. The knowledge of the nutritional characteristics of silages can improve the formulation of balanced rations, contributing to guarantee animal welfare and good productive performances.

Botanical and herbal supplements contain diverse compounds that support health and prevent disease by influencing biological pathways. In ruminant nutrition, such supplements are rarely used as most integrations focus on production improvements using synthetic additives. This review explores the potential of Mediterranean herbs, namely, garlic, oregano, and rosemary, in dairy ruminant diets, emphasizing their effects on milk production, antioxidant activity, weaning management, in vitro studies, and economic impacts. Using medicinal plants reduces reliance on synthetic additives, lowers costs, enhances animal health, and boosts productivity. These plants align with sustainable farming practices, enable premium market access, and support local resources to improve performance and reduce livestock’s environmental footprint.

In this study, we assessed the interspecific “social buffering effect” of humans on horses, exploring how human presence influences stress responses in horses in an unfamiliar environment using the “isolation paradigm.” We examined nine Haflinger horses under two counterbalanced conditions: with a passive human stranger ( social condition) or alone ( isolation condition). Stress responses were assessed through cortisol measurements, heart rate monitoring, and behavioral observations. While cortisol levels significantly increased in both conditions, with no notable differences before and after the tests, heart rate data revealed a different pattern. Results indicated that stress generally decreased in both scenarios, impacting heart rate. Initially, during the first five minutes, heart rate was significantly higher in the social condition compared to isolation, but this trend reversed in the following intervals, with heart rate significantly decreasing as interaction with the stranger increased. Positive interaction between time and stranger-directed behaviors suggested the stranger’s influence on heart rate strengthened over time. Overall, these finding suggest that while cortisol data did not reflect a social buffering effect, other metrics indicated that human presence effectively reduced stress in horses after a brief adjustment period, supporting the hypothesis that horses can benefit from human presence during stress, after a short adaptation time. This study highlights the complex nature of stress responses in horses and the potential role of humans as social buffers in interspecific contexts.

Determination of digestibility represents the first step for the evaluation of the net energy content of feed for livestock animals. The aim of this study was to evaluate the in vivo digestibility and in vitro degradability of five diets characterized by different forage/concentrate ratios (F:C) in horses. The in vitro degradability was determined by the Gas Production Technique (GPT), using as an inoculum source the feces of the same subjects used for the in vivo test. Five diets consisting of poliphyte hay, straw and grains of barley and oats with a different F:C ratio [90/10 (Diet 1); 78/22 (Diet 2); 68/32 (Diet 3); 60/40 (Diet 4); 50/50 (Diet 5) were formulated and administered in succession, starting with Diet 1. In the in vivo results, no significant differences emerged, despite the different F:C content. In in vitro fermentation, four diets out of the five (2, 3, 4, 5) presented a similar trend of the curve of gas production, showing good activity of the fecal micro population during the first hours of incubation. An important correlation between gas and Volatile Fatty Acid (VFA) were found, suggesting that the processes linked to the micro population deriving from the horse’s caecum follow metabolic pathways whose products can be modeled in the same way as for the rumen. The GPT could represent the correct method for studying the nutritional characteristics of feed for horses, using feces as the source of inoculum, even if further investigations must be performed to improve the technique.

Viticulture is vital to Italy’s agricultural sector, since it significantly contributes to the global wine industry. Microflora and microfauna are considered important factors for soil quality, improving grapevine growth, and promoting resistance to biotic and abiotic stresses. This study examined the impact of selected Trichoderma strains (T. harzianum M10 and T. afroharzianum T22) and their secondary metabolite 6-pentyl-α-pyrone (6PP) on the soil microbiome, the metabolome, and physiological changes of grapevines. Before treatment application, low levels of plant-parasitic nematodes (Rotylenchulus spp., Xiphinema pachtaicum) were found in the soil, together with pathogens (Fusarium spp., Neonectria spp.) and beneficial microbes (Clonostachys rosea, Pseudomonas spp.). Metagenomic analysis revealed significant treatment impacts in the soil microbiome, with T22 and 6PP treatments increasing Proteobacteria abundance, while slight variations of fungal communities and no significant differences in nematofauna were found. Metabolomic analysis showed that treatments induced grapevines to produce antioxidant secondary metabolites able to boost plant defense against abiotic and biotic stresses and increase nutraceutical grapes’ value. Finally, T22 treatment increased the grapes’ winemaking value, raising their Brix grade. Our results demonstrate that microbial or metabolite-based treatments could affect the soil microbiome composition, grapevine health and resilience, and grapes’ oenological and nutraceutical properties.