In this study, novel blends of mycelium biocomposites (MB) were developed. Various combinations of birch sawdust and hemp shives with birch bark (BB) and wheat bran (WB) additives were inoculated with basidiomycete Trametes versicolor to produce self-growing biomaterials. MB were characterized according to mycelial biomass increment in final samples, changes in chemical composition, elemental (C, H, N) analyses, granulometry of substrates, water-related and mechanical properties, as well as mold resistance and biodegradability. The mycelial biomass in manufactured MB increased by ~100% and ~50% in hemp and sawdust substrates, respectively. The lignocellulose ingredients during fungal growth were degraded as follows: cellulose up to 7% and 28% in sawdust and hemp substrates, respectively, and lignin in the range of 13% in both substrates. A larger granulometric fraction in hemp MB ensured higher strength property but weakened water absorption (600–880%) performance. Perspective MB combinations regarding strength performance were hemp/BB and pure hemp MB (σ10 0.19–0.20 MPa; E 2.9 MPa), as well as sawdust/WB combination (σ10 0.23 MPa; E 2.9 MPa). WB positively affected fungal biomass yield, but elevated water absorption ability. WB improved compressive strength in the sawdust samples but decreased it in the hemp samples. BB supplement reduced water absorption by more than 100% and increased the density of sawdust and hemp samples. All MB samples were susceptible to mold contamination after full water immersion, with identified fungal genera Rhizopus, Trichoderma and Achremonium. The MB exhibited high biodegradability after 12 weeks’ exposure in compost, and are therefore competitive with non-biodegradable synthetic foam materials.

Lignocellulosic biomass (LCB)-based thermal insulation materials available in the market are more expensive than conventional ones and consist mainly of wood or agricultural bast fibers which are primarily used in construction and textile industries. Therefore, it is crucial to develop LCB-based thermal insulation materials from cheap and available raw materials. The study investigates new thermal insulation materials from locally available residues of annual plants like wheat straw, reeds and corn stalks. The treatment of raw materials was performed by mechanical crushing and defibration by steam explosion process. Optimization of thermal conductivity of the obtained loose-fill thermal insulation materials was investigated at different bulk density levels (30–45–60–75–90 kg m⁻³). The obtained thermal conductivity varies in range of 0.0401–0.0538 W m⁻¹ K⁻¹ depending on raw material, treatment mode and a target density. The changes of thermal conductivity depending on density were described by the second order polynomial models. In most cases, the optimal thermal conductivity was revealed for the materials with the density of 60 kg m⁻³. The obtained results suggest the adjustment of density to achieve an optimal thermal conductivity of LCB-based thermal insulation materials. The study also approves the suitability of used annual plants for further investigation towards sustainable LCB-based thermal insulation materials.

This study investigates the mycelial biomass production and chitosan extraction potential of various Basidiomycota strains, including Heterobasidion annosum, Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, and Lentinus lepideus. Both submerged fermentation (SF) and solid-state fermentation (SSF) methods were employed. The chitosan yield in basidiocarps of Pleurotus ostreatus, Agaricus bisporus, and Ganoderma applanatum was also evaluated as a reference material. The chitosan extracted from fungal cells was characterized using elemental analyses and FTIR spectroscopy. Among the cultivated strains, P. chrysosporium exhibited the highest mycelial biomass concentration in SF (1.03 g 100 mL^–1) after 14 days, while T. versicolor achieved the highest biomass concentration in SSF (3.65 g 100 mL^–1). The highest chitosan yield was obtained from the mycelium of P. chrysosporium (0.38%) and T. versicolor (0.37%) in shaken SF. Additionally, commercially cultivated A. bisporus demonstrated the highest chitosan yield in fungal fruiting bodies (1.7%). The extracted chitosan holds potential as a functional biopolymer additive for eco-friendly materials, serving as an alternative to synthetic wet and dry strength agents in packaging materials.

The demand for eco-friendly packaging materials has urged researchers to look for alternatives to petroleum-based polymers. In this regard, paper-based products have turned out to be a promising choice; however, their weak resistance to water has limited their application. The use of various additives to enhance paper’s moisture resistance is a common practice. However, considering the growing global agenda for sustainable development, the search for new bio-based paper additives has become increasingly important. This study investigated the potential synergistic impact of the addition of nanofibrillated cellulose (NFC) and chitosan additives (CHIT) to different fiber combinations to improve paper’s properties, in particular, their wet strength. The efficacy of the additive application order was examined and was found to be crucial in achieving the desired outcomes. The results showed that incorporating CHIT after NFC enhanced the paper’s tensile and burst indicators, as well as the paper stretch in the dry state, by 35–70%, 35–55%, and 20–35%, respectively. In addition, the tensile index and stretch in the wet state improved 9–13 times and 2.5–5.5 times over, respectively. The air permeability decreased 2.5–12 times over. These findings demonstrate that the sequential addition of the NFC and CHIT additives yield a greater enhancement of paper’s properties than using each additive separately.

Bone fractures and bone defects affect millions of people every year. Metal implants for bone fracture fixation and autologous bone for defect reconstruction are used extensively in treatment of these pathologies. Simultaneously, alternative, sustainable, and biocompatible materials are being researched to improve existing practice. Wood as a biomaterial for bone repair has not been considered until the last 50 years. Even nowadays there is not much research on solid wood as a biomaterial in bone implants. A few species of wood have been investigated. Different techniques of wood preparation have been proposed. Simple pre-treatments such as boiling in water or preheating of ash, birch and juniper woods have been used initially. Later researchers have tried using carbonized wood and wood derived cellulose scaffold. Manufacturing implants from carbonized wood and cellulose requires more extensive wood processing—heat above 800 °C and chemicals to extract cellulose. Carbonized wood and cellulose scaffolds can be combined with other materials, such as silicon carbide, hydroxyapatite, and bioactive glass to improve biocompatibility and mechanical durability. Throughout the publications wood implants have provided good biocompatibility and osteoconductivity thanks to wood’s porous structure.

A significant increase in the consumption of recycled fiber products has been observed worldwide, and the industry is forced to solve the challenges of recycled fiber quality and add strength agents and/or virgin fibers to reach sufficient properties. In order to investigate whether the mechanical and air permeability properties of waste fiber (WF) material can be significantly improved by adding wood kraft fibers (KF), hemp soda fibers (HF), and ammonium persulfate oxidated cellulose nanofibrils (CNF), different fiber blends were prepared and tested. Results revealed the excellence of hemp fibers over wood fibers regarding the improvement of WF products. The results of WF after the addition of 10% mixed fibers (KF + HF) were higher than the results of a 50% KF addition. The impact of CNF depended on fiber composition and properties. A formula for modeling the CNF impact on different fiber compositions was proposed. Obtained fiber material samples showed suitability for home composting, thus contributing to the goals of the European Green Deal regarding reducing landfill waste and the development of cleaner products.