Here, we describe and discuss a method based on microscopical field of view (FOV) area to estimate diatom absolute abundances (densities or concentrations), and we statistically verify its reliability, also comparing it to another commonly used method (microspheres). To test the new method, we purposely performed replicate counts, both with the FOV and with the microsphere method, on both subfossil and recent material (samples) from mires. Intraclass correlation (ICC) revealed a high degree of agreement between the measurements obtained for the replicates with each of the two methods, suggesting that both are reliable for measuring diatom valve concentrations. However, the FOV consistently overestimated diatom absolute abundances, as compared to the microsphere method, and the ICC value used to assess the reliability of the two methods combined suggested that the two methods cannot be used interchangeably. The FOV method is relatively simple, has a lower cost, wider applicability, higher resolution, and warrants compatibility with existing datasets. However, there may also be drawbacks, such as the potential for sample distortion during the concentration process. Therefore, it is important to carefully evaluate the strengths and limitations of the different methods before adopting one for specific research or applied questions.

The transition from a perennial to an intermittent regime in newly intermittent rivers (nIRs) negatively affects both taxonomic and functional diversity, with significant repercussions on freshwater ecosystem processes and services. However, to better understand how changes in the natural flow regime may influence the structure and functioning of freshwater ecosystems, it is fundamental to assess variations in abiotic and biotic parameters throughout the hydrological phases characterizing nIRs. For these reasons, we evaluated the temporal changes in community structure and composition during the drying phase of a Central Apennines stream (Italy) over two consecutive drought years. We demonstrated that the different hydrological regime of the pre-drought phase profoundly affected the structure and composition of freshwater communities. The reduced discharge during the low-flow conditions of 2024 led to a transition from insect- to non-insect-dominated communities, with small-sized, lentic-adapted and generalist taxa replacing rheophile and more sensitive insect taxa. We also found marked interannual differences in temporal beta diversity. However, in both years, taxa richness did not exhibit a negative stepped response pattern during the sequence of channel contraction, flow cessation and pools formation. Consequently, we can assume that in newly intermittent Apennine rivers, the response of freshwater communities to drying is strictly dependent on the local and interannual variable hydrological context. This study emphasizes the need for further investigation to better understand the ecological impacts of increasing intermittence in formerly perennial streams and rivers.

Mires are significant C-sinks and important habitats for biodiversity conservation. Particularly in the Alpine peatlands, grazing and trampling cause mires to degrade, causing changes in plant communities that increase bulk density, peat erosion, and nutrient excesses. We took sixteen samples of epipelic and epiphytic diatoms from mire pools subjected to varying degrees of grazing in the Adamello-Brenta Nature Park (Trento, Italy). We identified >100 diatom species (ca. 70% included in Red List threat categories). We used nMDS to identify groups of diatom species. Their statistical significance was checked with an ANOSIM and, to identify the species that contributed most to the difference between the two groups, a BEST-SIMPER procedure was carried out. The multivariate analyses allowed us to identify significant differences among the most and least grazed sites, and to select the species that contributed most to this distinction. We confirmed that some species were more frequent and abundant in the most grazed sites and behaved as opportunists in the presence of a greater nutrient input, to the detriment of rare and sensitive species, which were more numerous and abundant in the less grazed sites. We provided useful information on grazing effects, underlining the importance of managing and protecting habitats of unique environmental value.

In many EU countries, a large proportion of domestic effluents is treated in small, decentralized wastewater treatment plants (WWTPs) which often lack appropriate treatment technologies. The low efficiency of these systems and their incorrect maintenance often contribute to environmental deterioration, with a large quantity of inadequately treated sewage dispersed in the soil or discharged into superficial watercourses. In the Abruzzo region (Central Italy), almost all rivers and streams receive wastewater from Imhoff tanks with only primary treatments. The ecological impacts of these effluents have rarely been investigated. This paper aimed to evaluate the response of freshwater invertebrates to Imhoff effluents in receiving watercourses with different ecological status. Our results showed that wastewater from Imhoff plants negatively affected the structure and composition of freshwater communities, with a reduction in the richness and abundance of more sensitive taxa and an increase in the most tolerant ones. These negative effects were more accentuated during low flow periods and in streams with moderate ecological status. To improve the ecological status of rivers and streams and to limit the diffusion of pathogens and micropollutants in freshwater, a more efficient (secondary treatments, possibly with nature-based solutions) and sustainable (water reuse or sewage sludge recycling) approach to wastewater treatment in decentralized WWTPs is urgently needed.