Given the limited understanding of species diversity and ecological preferences of diatoms of the genus Navicula in spring ecosystems, herein we present and describe as species new to science, Navicula aquaesuavis Lange-Bert., Levkov, Cid-Rodríguez, A.A.Saber and Cantonati sp. nov. This species was collected from a mountain spring located above the tree line at 1613 m a.s.l. in the Northern Apennines. The Fontana del Vescovo (Bishop’s spring), which is the locus classicus of the new species, has a low conductivity (60–70 µS cm⁻¹), temperature of ca. 5 °C, circumneutral pH (7.3–7.5), relatively low nitrate (ca. 1 mg L⁻¹), and also suffered from a discharge reduction from 1 to 0.1 L s⁻¹ from 2011 to 2023. The putative new species was confirmed by a second finding in Northern Macedonia, and we thoroughly document this second population as well. We seized the opportunity to describe this new Navicula and review the global literature on the diatom genus Navicula in spring ecosystems. Using the results of this review and our own databases on springs and wells in central Europe and Egypt, we discuss the main Navicula species and their environmental preferences in spring habitats.

Peatlands play a crucial role in carbon (C) sequestration and biodiversity conservation. However, these environments are highly vulnerable, and Europe has lost >60 % of its peatland habitat in recent decades. Cattle grazing and trampling contribute to peatland degradation, which generally result in a shift from moss-dominated vegetation to vascular plants and in lower C sequestration rates. Overgrazing poses also a significant threat to habitat integrity and biodiversity, especially in the Alpine area, where close-to-pristine mires with high ecological integrity are becoming extremely rare. Thus, a more in depth understanding of how cattle grazing and trampling are threatening Alpine mires is strongly needed for a sustainable management and conservation of these habitats. The objective of this study was to examine the impact of grazing on the physical, chemical, and biological characteristics of peat, with a focus on diatoms. To answer such a question, seven 50-cm deep cores were collected from mires located in the Adamello-Brenta Nature Park (North of Italy) along a grazing-induced disturbance gradient. Results indicated that grazing primarily affected at least the upper 15 cm of the peat, resulting in increased density and reduced water content, due to compaction, and lower C-to‑nitrogen ratio, possibly caused by both cow manure inputs and increased peat mineralization. Moreover, almost 200 diatom taxa were recorded across the 7 cores, with several of them falling under threat categories in the Red List for central Europe. The higher percentage of eutraphentic species in highly-grazed areas was related to the increase in nutrients caused by cattle manure. Finally, intense grazing increased the share of taxa that are more likely to survive in environments with unstable water availability (= aerial species). We showed that diatom data, supported by physical and chemical parameters, can be a refined tool to inform mire protection and rehabilitation.

Here, we describe and discuss a method based on microscopical field of view (FOV) area to estimate diatom absolute abundances (densities or concentrations), and we statistically verify its reliability, also comparing it to another commonly used method (microspheres). To test the new method, we purposely performed replicate counts, both with the FOV and with the microsphere method, on both subfossil and recent material (samples) from mires. Intraclass correlation (ICC) revealed a high degree of agreement between the measurements obtained for the replicates with each of the two methods, suggesting that both are reliable for measuring diatom valve concentrations. However, the FOV consistently overestimated diatom absolute abundances, as compared to the microsphere method, and the ICC value used to assess the reliability of the two methods combined suggested that the two methods cannot be used interchangeably. The FOV method is relatively simple, has a lower cost, wider applicability, higher resolution, and warrants compatibility with existing datasets. However, there may also be drawbacks, such as the potential for sample distortion during the concentration process. Therefore, it is important to carefully evaluate the strengths and limitations of the different methods before adopting one for specific research or applied questions.

Cymbella ectorii sp. nov. is a new freshwater diatom described from phytobenthos samples of Miño River (Galicia, NW of Iberian Peninsula). This new diatom is illustrated and discussed based on different samples collected in July-August 2015 in Miño River as it passes through the city of Ourense and was found four years later (2019) at the same sites. Cymbella ectorii sp. nov. is placed in the genus Cymbella due to shared features such as asymmetry about the apical axis, deflection of the external terminal raphe fissures towards the dorsal margin, and the presence of apical pore fields and stigmata. This taxon was compared with other Cymbella taxa, and its morphological features are documented through light and scanning electron micrographs. Cymbella ectorii sp. nov. is characterized by solitary cells, moderately dorsiventral and broadly lanceolate valves, a distinctly convex dorsal margin and a moderately convex ventral margin. Ends are not protracted but are slightly subrostrate and bluntly rounded. This taxon may be confused with other taxa such as Cymbella tumidula var. tumidula, C. tumidula var. procera, C. tropica, C. affinis, C. turgidula and Cymbopleura heilprinensis in terms of length, stria density and outline. Cymbella ectorii sp. nov. was found in freshwater environments with high dissolved oxygen concentrations, low conductivity, neutral to slightly alkaline pH and low nitrate concentrations.

Peatlands are a major carbon (C) sink and represent important habitats for nature conservation because of the occurrence of specifically adapted organisms. Peatlands are typically nutrient-poor environments, and thus extremely sensitive to nitrogen (N) depositions. In fact, increasing N inputs can cause a shift from a Sphagnum moss- to a vascular plant-dominated vegetation as well as an alteration of the mire geochemistry which, in turn, affects the litter composition and decomposition rate. Peatland is an extremely fragile ecosystem: at the European level, >60% of this habitat type has been lost just in the last decades. In Alpine peatlands, in particular, overgrazing has been identified as a main problem for habitat integrity and biodiversity.In the present study, six Belarus (50-cm deep) cores were collected from several peatlands located in the Adamello-Brenta Nature Park (Trentino, Italy) mirroring a grazing-induced disturbance gradient. All cores were cut frozen into 3-cm sections, and analysed for dry density, water content, pH, EC, and elemental (CHNS) composition. Diatom taphocoenoses were also determined. In particular, diatom frustules were prepared using hot hydrogen peroxide and finally mounted in the Naphrax© resin to produce permanent mounts for identifications and counts. 400 valves were counted and identified to the species level in each slide using a light microscope at x1000 magnification. The whole procedure was kept quantitative to allow the calculation of absolute abundances (n-valves/g-peat-dw).Physical, chemical and biological parameters generally underlined how the grazing influence was clear mainly in the top 20 cm, and resulted in an increasing of density (up to 2×) and N concentration (up to 3×) as well as in a lower gravimetric water content (up to 50%). No significant differences were observed below 30 cm of depth.  More than 100 diatom species were identified throughout the 6 cores investigated, and several of them are included in threat categories of the Red List for central Europe (e.g., Cymbopleura valaiseana, Eunotia hexaglyphis, E. triodon). Since diatoms can reflect major regional environmental gradients, they can be used as indicators of ecological conditions in peatlands. In fact, some species that are trivial (= frequent and often abundant) in mire environments in spite of being relatively rare at the overall level of European inland waters (e.g., Aulacoseira alpigena, Encyonema perpusillum, Kobayasiella micropunctata) were found in the cores from highly-grazed areas whilst several rare and sensitive species were detected only (or were clearly more frequent) in cores from mildly-grazed areas.This research can provide useful indications on the harmful effects of grazing in terms of both biogeochemical cycles and nature/habitats conservation.

The Mediterranean Region holds significant ecological importance, characterised by its unique climate, biodiversity and the crucial role it plays in global ecosystems. Mediterranean streams are naturally highly-stressed environments mainly due to fluctuations in water quantity. River flow generally varies from perennial to ephemeral and temporary rivers constitute significant water resources. Streams that flow through Balearic Islands are subjected to these conditions. The majority of these streams sustain water flow for 4–5 months annually, with exceptions noted for streams associated with springs, which typically maintain water throughout most of the year. Benthic diatoms are widely recognised as reliable bioindicators of water quality, used in many aquatic ecosystems. Analysing diatom communities and their biodiversity serves as a valuable tool to ensure the ecological and sustainable utilisation of water resources as well as the accurate development of guidelines for their conservation.The field of diatom taxonomy and distribution plays a crucial role in advancing our understanding of aquatic ecosystems and their biodiversity. Species of the genus Nitzschia are extensively found throughout the Mediterranean Region, including the Balearic Islands. However, they have rarely been investigated in temporary streams. This study presents the first record of Nitzschia transtagensis Morales, Novais, Wetzel, Morais & Ector, outside the type locality and being the second record in Europe. In this study, the authors found this taxon in one temporary stream of Majorca Island, Torrent des Castellot in November 2005 (Balearic Islands). Nitzschia transtagensis occurred at 2.6% abundance in this stream with oligotrophic waters (0.052 mg∙l^-1 of nitrate), slightly alkaline pH values (7.8) and water conductivity levels of 626.5 µS cm^-1. This species was recorded in the biofilm of the stones together with other taxa such as Achnanthidium minutissimum (Kützing) Czarnecki (39.2%), Gomphonema rosenstockianum Lange-Bertalot & Reichardt (28.9%) and Halamphora oligotraphenta (Lange-Bertalot) Levkov (20.4%). The Nitzschia transtagensis frustules found in the examined material have similar dimensions and a higher fibulae count (8–11 in 10 μm vs. 6–10 in 10 μm) compared to the type material of Nitzschia transtagensis. The habitat characteristics in which this species was found are described, together with LM micrographs of this taxon.