In the context of agricultural soil degradation caused by the extensive use of chemical amendments, ecological alternatives with minimal detrimental impact on ecosystems are gaining popularity. Recent advancements in processing technologies have improved the quality and extraction efficiency of bioactive compounds, particularly when multiple conventional or innovative techniques are being used to potentially overcome the most common limitations. This paper proposes the development and testing of a hybrid technology design that employs two extraction techniques, namely ultrasound and high pressure, that can be used either separately or in tandem. An initial assessment of the prototype potential for isolating the desired compounds was made, by testing three various working regimens for the processing of a mixture of onion, pea, and soybean. By incorporating the bioactive compounds produced during the experimental phase in the seedling transplantation holes, we were able to test the potential of stimulating the development rate of vegetables and reducing the attack of pests. The extracts obtained using the hybrid technology showed positive results when used to reduce pest attacks (decreasing average attack frequency by 7%), however had negative effects when used to promote biostimulation, when acted as an inhibitor. The hybrid extraction approach improved the mass transfer into solvent by 14% when compared to high-pressure processing and by 7% when compared to sonication.

This paper presents studies performed for the monitoring of imidacloprid, clothianidin and thiamethoxam residues applied as seed treatment in rapeseed (Brassica napus ssp. oleifera), maize (Zea mays) and sunflower crops (Helianthus annuus). The experiments were located in representative areas of the mentioned crops. Residue levels were determined in plant samples at different phenological development stages, including flowers, as well as in bees and hive products (pollen, honeycomb, honey) by liquid chromatography/tandem mass spectroscopy (LC-MS/MS). The analyses were performed in ISO 17025-accredited laboratories, referring to the limit of quantification (LOQ), characteristic of the method used to determine the residues. In 2019, the percentage of samples that contained residues of the three substances, applied to the seed, was 16.39%, representing 20 samples out of the total of 122 analyzed samples. In 2020, 10 samples contained neonicotinoid residues above the LOQ, including 5 soil samples and 5 plant samples, representing 6.17% of the total samples. In 2021, from 149 samples with neonicotinoid applied as seed treatment, residues were identified in 12 soil samples and 11 plant samples, representing 15.43% of the total number of samples. In 2022, only 12 soil samples and 1 pasture sample contained residues above the LOQ. The results show that the highest percentage of samples with residues above the LOQ was recorded by the soil samples, while the flower and bee samples had the lowest percentages of samples with residues above the LOQ; no residues of the three neonicotinoid substances were identified in the honey samples.

Over the past years, increasing attention has been drawn to the adverse effects of agricultural pesticide use on pollinators, with honeybees being especially vulnerable. The aim of this study was to evaluate the levels of residues detectable and/or quantifiable of neonicotinoid pesticides and other pesticides in biological materials (bees, bee brood, etc.) and beehive products (honey, pollen, etc.) applied as seed dressings in rapeseed and sunflower plants in two growing seasons (2020–2021) in fields located in three agro-climatic regions in Romania. The study involved the comparative sampling of hive products (honey, pollen, adult bees, and brood) from experimental and control apiaries, followed by pesticide residue analysis in an accredited laboratory (Primoris) using validated chromatographic techniques (LC-MS/MS and GC-MS). Toxicological analyses of 96 samples, including bees, bee brood, honey, and pollen, confirmed the presence of residues in 46 samples, including 10 bee samples, 10 bee brood samples, 18 honey samples, and 8 pollen bread samples. The mean pesticide residue concentrations detected in hive products were 0.032 mg/kg in honey, 0.061 mg/kg in pollen, 0.167 mg/kg in bees, and 0.371 mg/kg in bee brood. The results highlight the exposure of honeybee colonies to multiple sources of pesticide residue contamination, under conditions where legal recommendations for the controlled application of agricultural treatments are not followed. The study provides relevant evidence for strengthening the risk assessment framework and underscores the need for adopting stricter monitoring and regulatory measures to ensure the protection of honeybee colony health.

Vacuum degassing of seeds is a basic preliminary stage of the treatment process to improve the viability of seeds of various crops. In this work, the degassing process of corn seeds was experimentally and numerically analyzed by removing air or other gases from around the seeds, specifically from the seed coating, in a rough vacuum chamber. Two complementary variants were employed to understand and optimize this process to improve the quality and germination rate of the seeds. The average germination percentage on the first day was about 98%, and the germination speed of 5.0 days. Several experiments were conducted with well-established durations of 10 min and masses of 5 kg and masses of corn seeds at different temperatures to observe and record the behavior of the system, facilitating the modeling of the degasification process in the vacuum compartment. Modeling the degasification operation in the vacuum chamber allowed for determining the pressure profiles on the vacuum chamber and its lid. Numerical simulations were either conducted using a simulation program developed in the Visual Basic Applications (VBA) language for Microsoft Excel to model the degassing process in the vacuum chamber or with the assistance of specialized software (transient structural analysis and simulation program in the ANSYS Workbench environment). Statistical analysis of the correlation between experimental and estimated pressure values revealed that both the proposed mathematical model and the solution method are well-chosen, with differences expressed through the absolute error (EA) being very small, only 1.425 mbar. Structural dynamic analysis carried through the Finite Element Method (FEM) highlights that the chosen materials for manufacturing the vacuum chamber vessel (316 stainless steel—yield strength 225 MPa and tangent modulus 2091 MPa) or the chamber lid (transparent acrylic plastic—yield strength 62.35 MPa and shear modulus 1445.3 MPa) are durable and capable of withstanding the desired pressure and temperature demands in the seed treatment process. Additionally, through structural dynamic analysis, it was possible to study the deformation of system components, providing a detailed perspective on their structural distribution. Thus, the paper aims to improve the quality and survival/germination rate of corn seeds as an important step to improve corn yield through simulations and analyses (numerical and experimental) of the vacuum corn seed degassing system. The degassing process of the vacuum chamber was simulated with a simulation program developed for Microsoft Excel for Microsoft 365 MSO (Version 2401 Build 16.0.17231.20236) 64-bit in the VBA language and software (transient structural dynamic analysis in the ANSYS environment through FEM). Vacuum degassing of corn seeds involves the removal of air or other gases around the seeds or products, which is crucial in various fields such as the food, pharmaceutical, or space technology industries.

The potato (Solanum tuberosum L.) is a globally cultivated staple food crop of great importance, both as a food plant and as an industrial and fodder plant. Significant annual losses in potato production are reported due to soilborne phytopathogens. Therefore, effective control or management of plant diseases is an essential component of sustainable agricultural practices. Alternaria species synthesize dangerous toxins, which can accumulate in infected agricultural crops and persist in processed products. Fungi belonging to the genus Trichoderma have been widely used as effective biological control agents against fungal phytopathogens. The present study represents a continuation of this research, focusing on the prevention and control of Alternaria diseases in potato cultivation.

Twenty-one clinically healthy honeybee colonies were monitored in a private apiary located in Vâlcea County, Romania, within an area influenced by electromagnetic fields (EMF) according to the National Authority for Management and Regulation in Communications (ANCOM) allocations. The site was situated below the activation threshold of the 5G n78 band (3.3–3.8 GHz), where mean field intensities of 12.74 mW/m² and peak values of 17.96 mW/m² were recorded. For each colony, five thermographic measurements were conducted (at the hive entrance, left lateral wall, rear wall, right lateral wall, and interior), totaling 105 recordings. During the inactive beekeeping season of 2025, strong colonies initiated winter clustering at approximately 7 °C, medium-strength colonies at 8 °C, and swarms at 10 °C, values consistent with the literature (7–10 °C). Thermographic analyses showed that only strong colonies consistently maintained the optimal internal range (32–36 °C), although peripheral zones exhibited peaks up to 52 °C. Medium-strength colonies displayed higher variability, with maxima of 52–55 °C, while swarms remained unstable, fluctuating around the minimum optimal temperature (≈25 °C) and frequently dropping to lower values (21–24 °C), confirming significant differences in thermoregulatory capacity among colony categories. Acoustic monitoring of the colonies revealed a nonlinear profile of sound level: ~36 dB at 50 m, maximum levels of 43–45 dB at 400–540 m due to reflections and interferences of EMF, and ~40 dB at 640–1000 m, with high variability influenced by terrain and nearby constructions.