This study has been based on the assumption that articular motion is an important aspect of mechanotransduction in synovial joints. For this reason a new bioreactor concept, able to reproduce joint kinematics more closely, has been designed. The prototype consists of a rotating scaffold and/or cartilage pin, which is pressed onto an orthogonally rotating ball. By oscillating pin and ball in phase difference, elliptical displacement trajectories are generated that are similar to the motion paths occurring in vivo. Simultaneously, dynamic compression may be applied with a linear actuator, while two-step-motors generate the rotation of pin and ball. The whole apparatus is placed in an incubator. The control station is located outside. Preliminary investigations at the gene expression level demonstrated promising results. Compared with free-swelling control and/or simply compression- loaded samples, chondrocyte-seeded scaffolds as well as nasal cartilage explants exposed to interface motion both showed elevated levels of cartilage oligomeric matrix protein mRNA. The final design of the bioreactor will include four individual stations in line, which will facilitate the investigation of motion-initiated effects at the contacting surfaces in more detail.

Although numerous in vitro studies report on the tribological performance of and, separately, on the corrosion properties of cobalt-based alloys in metal-on-metal (MoM) bearings, the few studies that take into account the synergistic interaction of wear and corrosion (tribocorrosion) have used canonical tribo-test methods. We therefore developed synergistic study using a test method that more closely simulates hip bearing conditions. (1) Is the total material loss during tribocorrosion larger than the sum of its components generated during isolated mechanical wear and isolated corrosion? (2) How is the tribocorrosive process affected by the presence of protein? High carbon CoCrMo alloy discs (18) were subjected to corrosion and tribocorrosion tests under potentiostatic conditions in an apparatus simulating hip contact conditions. The input variables were the applied potential and the protein content of the electrolyte (NaCl solution versus bovine serum, 30 g/L protein). The output variables were mass loss resulting from wear in the absence of corrosion, mass loss resulting from corrosion in the absence of wear, and the total mass loss under tribocorrosion, from which the additional mass loss resulting from the combined action of wear and corrosion, or synergism, was determined in the presence and absence of protein. The degradation mechanisms were sensitive to the interaction of wear and corrosion. The synergistic component (64 μg) in the presence of protein amounted to 34% of total material loss (187 μg). The presence of protein led to a 23% decrease in the total mass loss and to a considerable reduction in the mean current (4 μA to 0.05 μA) under tribocorrosion. Synergistic effects during tribocorrosion may account for a considerable portion of MoM degradation and are affected by proteins. The in vivo performance of some large-diameter MoM joints is unsatisfactory. The synergistic component resulting from tribocorrosion may have been missed in conventional preclinical wear tests.

He interaction of wear and corrosion, and putting the characteristics and the potential origin of wear particles in perspective with the proposed wear mechanisms. It then summarizes the current knowledge on the biological effects of particles and metal ions in relation to these wear mechanisms. Results: Tribochemical reactions play an important role in the wear of metal-on-metal joints. The generated tribomaterial, which progressively forms by mechanical mixing of the uppermost nanocrystalline zone of the metal surface with proteins from the synovial fluid, governs the wear rate and influences the corrosive behavior of the bearing. Nanometer-sized wear particles may initially originate from the passivation layer covering the implant surface and then detach from this tribolayer. The inflammatory response observed surrounding metal-on-metal implants appears to be lower than that around metal-on-polyethylene implants. However, metallic byproducts, which can complex with proteins, may lead to a T lymphocyte-mediated hypersensitivity response. Conclusions: The tribolayer appears to have beneficial effects on the wear rate. Much information has been gained on wear particle characteristics, but the exact mechanisms of particle detachment remain to be further elucidated. Excessive wear along with a hypersensitivity response may be at the origin of the early adverse tissue reactions that have been recently reported in some patients with metal-on-metal implants. Clinical Relevance: Future development of new methods to improve the tribolayer retention and optimize the tribocorrosive properties of the implant may minimize the clinical impact of implant wear and immune responses. This article was updated on May 13, 2011, because of previous editorial errors....