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Plants produce several chemically diverse bioactive substances that may influence the growth and development of other organisms when released into the environment in a phenomenon called allelopathy. Several of these allelopathic species also have reported medicinal properties. In this study, the potential allelopathic effects of more than a hundred medicinal plants from Cambodia were tested using the dish pack method. The dish pack bioassay method specifically targets volatile allelochemicals. Twenty-five species were found to have significant inhibitory effects on lettuce radicle growth. Eleven different plant families, including Iridaceae (2), Apocynaceae (2), Poaceae (2), Sapindaceae, Araceae, Combretaceae, Orchidaceae, Clusiaceae, Zingiberaceae, Rutaceae and Asparagaceae had the plant species with high inhibitory effects. Allophyllus serrulatus had the highest growth inhibitory effect on lettuce radicles more than 60%, followed by Alocasia macrorrhiza, Iris pallida, Terminalia triptera, Wrightia tomentosa, Cymbidium aloifolium, Garcinia villersiana and Kaempferia parviflora. The candidate species were subjected to further studies to identify the volatile allelochemicals in the volatile constituents.
Yourk Sothearith; Kwame Sarpong Appiah; Hossein Mardani; Takashi Motobayashi; Suzuki Yoko; Khou Eang Hourt; Akifumi Sugiyama; Yoshiharu Fujii. Determination of the Allelopathic Potential of Cambodia’s Medicinal Plants Using the Dish Pack Method. Sustainability 2021, 13, 9062 .
AMA StyleYourk Sothearith, Kwame Sarpong Appiah, Hossein Mardani, Takashi Motobayashi, Suzuki Yoko, Khou Eang Hourt, Akifumi Sugiyama, Yoshiharu Fujii. Determination of the Allelopathic Potential of Cambodia’s Medicinal Plants Using the Dish Pack Method. Sustainability. 2021; 13 (16):9062.
Chicago/Turabian StyleYourk Sothearith; Kwame Sarpong Appiah; Hossein Mardani; Takashi Motobayashi; Suzuki Yoko; Khou Eang Hourt; Akifumi Sugiyama; Yoshiharu Fujii. 2021. "Determination of the Allelopathic Potential of Cambodia’s Medicinal Plants Using the Dish Pack Method." Sustainability 13, no. 16: 9062.
The continuous planting of Japanese pear leads to a soil sickness syndrome that eventually affects the growth and yield of the plant. In this study, we aimed to elucidate the characteristics of soil sickness syndrome in the Japanese pear and construct countermeasures using the rhizosphere soil assay method that can quantify the risk of soil sickness syndrome by inhibitory chemicals. Water flushing treatment, rainfall treatment, and the incorporation of test soils with different rates of activated carbon were evaluated on the risks of soil sickness. The water flushing treatment under laboratory conditions and exposure of the continuous cropping soil to rainfall in the open field decreased the inhibition rate of the soil. The decrease in soil inhibition rate was presumed to be the result of accumulated growth inhibitory substances in the soil being washed away by water. In addition, activated carbon with the potential to reduce the soil sickness syndrome was selected using the rhizosphere soil assay method. It was clarified that the mixing of the selected activated carbon with the continuous cropping soil reduced the inhibition rate and increased the growth of pear trees increased compared to the untreated soil from the continuous cropping field. The inhibition rate of the soil from the continuous cropping field was reduced to the level of soil with no history of Japanese pear cultivation. In the replanted field, these treatments can promote the growth of trees by reducing the influence of soil sickness syndrome.
Tomoaki Toya; Masayoshi Oshida; Tatsuya Minezaki; Akifumi Sugiyama; Kwame Appiah; Takashi Motobayashi; Yoshiharu Fujii. Elucidation of the Characteristics of Soil Sickness Syndrome in Japanese Pear and Construction of Countermeasures Using the Rhizosphere Soil Assay Method. Agronomy 2021, 11, 1468 .
AMA StyleTomoaki Toya, Masayoshi Oshida, Tatsuya Minezaki, Akifumi Sugiyama, Kwame Appiah, Takashi Motobayashi, Yoshiharu Fujii. Elucidation of the Characteristics of Soil Sickness Syndrome in Japanese Pear and Construction of Countermeasures Using the Rhizosphere Soil Assay Method. Agronomy. 2021; 11 (8):1468.
Chicago/Turabian StyleTomoaki Toya; Masayoshi Oshida; Tatsuya Minezaki; Akifumi Sugiyama; Kwame Appiah; Takashi Motobayashi; Yoshiharu Fujii. 2021. "Elucidation of the Characteristics of Soil Sickness Syndrome in Japanese Pear and Construction of Countermeasures Using the Rhizosphere Soil Assay Method." Agronomy 11, no. 8: 1468.
Phnom Kulen National Park, in north-western Cambodia, has huge richness in biodiversity and medicinal value. One hundred and ninety-five (195) medicinal plant species were collected from the national park to examine allelopathic potentials by using the sandwich method, a specific bioassay for the evaluation of leachates from plants. The study found 58 out of 195 medicinal plant species showed significant inhibitory effects on lettuce radicle elongation as evaluated by standard deviation variance based on the normal distribution. Three species including Iris pallida (4% of control), Parabarium micranthum (7.5% of control), and Peliosanthes teta (8.2% of control) showed strong inhibition of lettuce radicle elongation less than 10% of the control. The results presented could present as a benchmark for isolation and identification of allelochemicals among medicinal plants used in Cambodia.
Yourk Sothearith; Kwame Appiah; Takashi Motobayashi; Izumi Watanabe; Chan Somaly; Akifumi Sugiyama; Yoshiharu Fujii. Evaluation of Allelopathic Potentials from Medicinal Plant Species in Phnom Kulen National Park, Cambodia by the Sandwich Method. Sustainability 2020, 13, 264 .
AMA StyleYourk Sothearith, Kwame Appiah, Takashi Motobayashi, Izumi Watanabe, Chan Somaly, Akifumi Sugiyama, Yoshiharu Fujii. Evaluation of Allelopathic Potentials from Medicinal Plant Species in Phnom Kulen National Park, Cambodia by the Sandwich Method. Sustainability. 2020; 13 (1):264.
Chicago/Turabian StyleYourk Sothearith; Kwame Appiah; Takashi Motobayashi; Izumi Watanabe; Chan Somaly; Akifumi Sugiyama; Yoshiharu Fujii. 2020. "Evaluation of Allelopathic Potentials from Medicinal Plant Species in Phnom Kulen National Park, Cambodia by the Sandwich Method." Sustainability 13, no. 1: 264.
Weed management is an important issue since weeds directly compete with crop plants for space, nutrients; serve as habitat for insect pests and diseases, and can create a significant annual reduction in crop productivity. This study focused on evaluating the contribution of the secondary metabolites of the fruit pulp of Couroupita guianensis Aubl. for its potential growth inhibitory effect. Crude extracts of C. guianensis fruit pulp were collected with different solvents and applied to test plants in petri dishes. The crude extracts of methanol and 1% dimethyl sulfoxide (DMSO) showed potential growth inhibitions with the 50% effective concentration (EC50) of 223 and 229 µg/mL in the bioassay experiment. In the greenhouse pot experiment, soil incorporated with oven-dried fruit pulp of C. guianensis was evaluated on cultivated plant species including Lactuca sativa L., Trifolium repens L., Medicago sativa L., Lolium multiflorum Lam., and Phleum pratense L. The incorporation of dried fruit pulp of C. guianensis into soil reduced shoot and root lengths and the germination percentage of test plants. It was observed that the monocot plants were more affected than the dicot plants. The fruit pulp of C. guianensis was subjected to reversed-phase high-performance liquid chromatography (HPLC) analysis to identify the active compounds. Indigo, identified as one of the candidate compounds of the C. guianensis, had high specific activity (i.e., strong inhibitory activity) in a phytotoxicity bioassay and could explain through the total activity concept the growth inhibitory effect of the C. guianensis on test plants. The results suggested that indigo has plant growth inhibitory effect, indicating the allelopathic potential of C. guianensis, which could be exploited in sustainable weed management.
Kohinoor Begum; Takashi Motobayashi; Nazmul Hasan; Kwame Sarpong Appiah; Mashura Shammi; Yoshiharu Fujii. Indigo as a Plant Growth Inhibitory Chemical from the Fruit Pulp of Couroupita guianensis Aubl. Agronomy 2020, 10, 1388 .
AMA StyleKohinoor Begum, Takashi Motobayashi, Nazmul Hasan, Kwame Sarpong Appiah, Mashura Shammi, Yoshiharu Fujii. Indigo as a Plant Growth Inhibitory Chemical from the Fruit Pulp of Couroupita guianensis Aubl. Agronomy. 2020; 10 (9):1388.
Chicago/Turabian StyleKohinoor Begum; Takashi Motobayashi; Nazmul Hasan; Kwame Sarpong Appiah; Mashura Shammi; Yoshiharu Fujii. 2020. "Indigo as a Plant Growth Inhibitory Chemical from the Fruit Pulp of Couroupita guianensis Aubl." Agronomy 10, no. 9: 1388.
The inhibitory activities of the leachates and volatiles from 53 plant species (spices and herbs) were evaluated against lettuce (Lactuca sativa “Great Lakes 366”) seedling growth using the sandwich and dish pack methods, respectively. With the sandwich method, parsley (Petroselinum sativum) showed the strongest inhibitory effect on lettuce radicle growth (77%), followed by tarragon (Artemisia dracunculus) (72%). However, caraway (Carum carvi), dill (Anethum graveolens) (seed), laurel (Laurus nobilis), rosemary (Rosmarinus officinalis), and sage (Salvia officinalis) were the most inhibitory species (100% inhibition of lettuce radicle and hypocotyl growth inhibition at all distance wells) in the dish pack method. Cardamom (Elettaria cardamomum) and thyme (Thymus vulgaris) also showed strong inhibitory activity (100% for radicle and hypocotyl growth inhibition at all 41 and 58 mm distance wells). The headspace sampling and gas chromatography-mass spectrometry (GC-MS) analysis identified the main inhibitory active compounds as carvone in caraway and dill (seeds), 1,8-cineole in laurel and cardamom, and borneol in thyme. Both camphor and 1,8-cineole were detected in rosemary and sage, and the total activity evaluation showed that camphor was the major inhibitory compound in rosemary, although both compounds played equal roles in sage.
Takayuki Sekine; Kwame Sarpong Appiah; Majid Azizi; Yoshiharu Fujii. Plant Growth Inhibitory Activities and Volatile Active Compounds of 53 Spices and Herbs. Plants 2020, 9, 264 .
AMA StyleTakayuki Sekine, Kwame Sarpong Appiah, Majid Azizi, Yoshiharu Fujii. Plant Growth Inhibitory Activities and Volatile Active Compounds of 53 Spices and Herbs. Plants. 2020; 9 (2):264.
Chicago/Turabian StyleTakayuki Sekine; Kwame Sarpong Appiah; Majid Azizi; Yoshiharu Fujii. 2020. "Plant Growth Inhibitory Activities and Volatile Active Compounds of 53 Spices and Herbs." Plants 9, no. 2: 264.
This study focuses on identifying volatile emissions from plants with potential plant growth inhibitory activity. The aim of this study was to evaluate plant species from the Asian country Bangladesh for new, potential volatile allelopathic species. A total of 103 plant samples from 40 different families were assessed with the dish pack (DP) method. About 25% of the evaluated plant samples influenced (inhibited or stimulated) the growth of lettuce, due to the presence of potentially volatile allelochemicals. The pericarp of Sapindus mukorossi Gaertn. caused the lowest radicle elongation (3% of control) of the lettuce. This was followed by the leaves of Cassia nodosa Roxb. (34.4%) and the root of Kaempferia galangal L. (43.4%), in that order. Therefore, the pericarp of S. mukorossi is reported from this study as a new potential volatile allelopathic species. On the contrary, the leaves of Gynostemma pentaphyllum Thunb. had a stimulatory effect on the hypocotyl elongation of lettuce seedlings (156% of control). The single petri dish (SPD), a new method, was also adapted to justify the potentiality of the growth control of particular allelopathic species. This study revealed that the new potentially volatile allelopathic plant species could be further explored in sustainable weed management.
Kohinoor Begum; Mashura Shammi; Nazmul Hasan; Kwame Sarpong Appiah; Yoshiharu Fujii. Evaluation of Potential Volatile Allelopathic Plants from Bangladesh, with Sapindus mukorossi as a Candidate Species. Agronomy 2019, 10, 49 .
AMA StyleKohinoor Begum, Mashura Shammi, Nazmul Hasan, Kwame Sarpong Appiah, Yoshiharu Fujii. Evaluation of Potential Volatile Allelopathic Plants from Bangladesh, with Sapindus mukorossi as a Candidate Species. Agronomy. 2019; 10 (1):49.
Chicago/Turabian StyleKohinoor Begum; Mashura Shammi; Nazmul Hasan; Kwame Sarpong Appiah; Yoshiharu Fujii. 2019. "Evaluation of Potential Volatile Allelopathic Plants from Bangladesh, with Sapindus mukorossi as a Candidate Species." Agronomy 10, no. 1: 49.
Turkey has one of the richest plant diversities in the Mediterranean region. In the current literature, no broad screening has been conducted on the potential allelopathy of plants from Turkey. This study aimed to evaluate the allelopathic activity of a large number of plants from Turkey for the first time and to determine the species with significant plant growth inhibitory potentials by bioassay. Dried samples of different plant parts were collected from local herbalists. The sandwich method was used to evaluate the potential allelopathy of 126 medicinal plants belonging to 55 families. The results of lettuce radicle and hypocotyl growth for 10 and 50 mg sample treatment conformed to normal distribution. Significant inhibition on lettuce radicle elongation with 10 mg sample was observed in 40 species, out of which 27 species showed over 50% inhibitory activity. The results suggested that these species could contain potential inhibitory compounds against lettuce radicle or hypocotyl growth. The calyxes of Hibiscus sabdariffa (3.2% of control) and the seeds of Prunus dulcis (5.7% of control) showed the most potent growth inhibitory activity on lettuce radicle elongation. The potential plant growth inhibitory effects of these plants, together with the fruits of Rhus coriaria and seeds of Prunus mahaleb, have been reported in this study for the first time. All these plants are medicinal, and the results hereby presented provide essential information about the allelopathic effects of medicinal plants from Turkey.
Tugba Gonca Isin Ozkan; Emine Akalin Urusak; Kwame Sarpong Appiah; Yoshiharu Fujii; Isin Ozkan. First Broad Screening of Allelopathic Potential of Wild and Cultivated Plants in Turkey. Plants 2019, 8, 532 .
AMA StyleTugba Gonca Isin Ozkan, Emine Akalin Urusak, Kwame Sarpong Appiah, Yoshiharu Fujii, Isin Ozkan. First Broad Screening of Allelopathic Potential of Wild and Cultivated Plants in Turkey. Plants. 2019; 8 (12):532.
Chicago/Turabian StyleTugba Gonca Isin Ozkan; Emine Akalin Urusak; Kwame Sarpong Appiah; Yoshiharu Fujii; Isin Ozkan. 2019. "First Broad Screening of Allelopathic Potential of Wild and Cultivated Plants in Turkey." Plants 8, no. 12: 532.
One hundred and thirty-nine medicinal plant species were screened for their allelopathic activity through volatile emissions using Lactuca sativa as a test plant. Volatile emissions from the leaves of star anise (Illicium verum) showed the highest inhibition (100%) on the radicle and hypocotyl growth. Using headspace gas collection and gas chromatography-mass spectrometry (GC-MS), seven major volatile compounds from the leaves of star anise, including α-pinene, β-pinene, camphene, 1,8-cineole, D-limonene, camphor, and L-fenchone were detected. To determine volatile compounds that may contribute to the inhibitory activity of star anise, the allelopathic potential of individual volatiles from star anise was evaluated using the cotton swab bioassay. The EC50 was calculated for each of the seven identified compounds. L-fenchone showed the strongest growth inhibitory activity (EC50 is 1.0 ng/cm3 for radicle and hypocotyl growth of lettuce), followed by 1,8-cineole, and camphene. This is the first report that L-fenchone could be an important volatile allelochemical from the leaves of star anise. From the actual concentration of each volatile compound in headspace and EC50 value, we concluded that the four volatile compounds, including L-fenchone, 1,8-cineole, β-pinene, and camphene are the most important contributors to the volatile allelopathy of star anise.
Gaowa Kang; Maryia Mishyna; Kwame Sarpong Appiah; Masaaki Yamada; Akihito Takano; Valery Prokhorov; Yoshiharu Fujii. Screening for Plant Volatile Emissions with Allelopathic Activity and the Identification of L-Fenchone and 1,8-Cineole from Star Anise (Illicium verum) Leaves. Plants 2019, 8, 457 .
AMA StyleGaowa Kang, Maryia Mishyna, Kwame Sarpong Appiah, Masaaki Yamada, Akihito Takano, Valery Prokhorov, Yoshiharu Fujii. Screening for Plant Volatile Emissions with Allelopathic Activity and the Identification of L-Fenchone and 1,8-Cineole from Star Anise (Illicium verum) Leaves. Plants. 2019; 8 (11):457.
Chicago/Turabian StyleGaowa Kang; Maryia Mishyna; Kwame Sarpong Appiah; Masaaki Yamada; Akihito Takano; Valery Prokhorov; Yoshiharu Fujii. 2019. "Screening for Plant Volatile Emissions with Allelopathic Activity and the Identification of L-Fenchone and 1,8-Cineole from Star Anise (Illicium verum) Leaves." Plants 8, no. 11: 457.
The present study aimed to examine the phytotoxic potential of seven Vietnamese tea samples based on the specific and total activity of caffeine and tea extracts on test plants. The sandwich method results indicated that the inhibitory effect of tea samples on the radicle and hypocotyl growth of lettuce seedlings was dependent on the concentration and type of tea samples, and also the presence of agar soluble allelochemicals. Among the seven tea samples, the leachates from Vinatea-green tea showed the highest inhibition on the radicle growth of lettuce seedlings with 50% suppression at 0.12 mg dry leaves/mL of agar. Caffeine concentration in tea samples analyzed by high-performance liquid chromatography (HPLC) varied from 20.7 to 38.2 µg/mL of dry leaves. The specific activity (EC50 value) of pure caffeine was 75 µg/mL, and the highest total activity of caffeine estimated in Vinatea-green tea was 0.51 [no unit]. Caffeine from green and oolong tea may be considered as one of the contributors to the inhibitory activity of the crude extract. Moreover, the phytotoxicity of pure caffeine and aqueous tea extracts was highly selective on the growth of different plant species. The concentration of caffeine detected from tea farm soil ranged from 0.137 to 0.145 µg/g soil. The results indicated that caffeine might be considered as a promising allelochemical from Vietnamese tea and can be a good candidate for weed management.
Van Thi Thanh Pham; Tamer Ismail; Maryia Mishyna; Kwame Sarpong Appiah; Yosei Oikawa; Yoshiharu Fujii. Caffeine: The Allelochemical Responsible for the Plant Growth Inhibitory Activity of Vietnamese Tea (Camellia sinensis L. Kuntze). Agronomy 2019, 9, 396 .
AMA StyleVan Thi Thanh Pham, Tamer Ismail, Maryia Mishyna, Kwame Sarpong Appiah, Yosei Oikawa, Yoshiharu Fujii. Caffeine: The Allelochemical Responsible for the Plant Growth Inhibitory Activity of Vietnamese Tea (Camellia sinensis L. Kuntze). Agronomy. 2019; 9 (7):396.
Chicago/Turabian StyleVan Thi Thanh Pham; Tamer Ismail; Maryia Mishyna; Kwame Sarpong Appiah; Yosei Oikawa; Yoshiharu Fujii. 2019. "Caffeine: The Allelochemical Responsible for the Plant Growth Inhibitory Activity of Vietnamese Tea (Camellia sinensis L. Kuntze)." Agronomy 9, no. 7: 396.
Weed management is one of the significant challenges of field crops since weeds pose a remarkable threat to crop productivity in South Asian countries, including Bangladesh. Allelopathy, a phenomenon whereby secondary metabolites produced and released by one plant species influence the growth and development of other species can be exploited in sustainable management. The focus of this study was to evaluate potential allelopathic plant species which can be further explored as alternatives to synthetic herbicides or incorporated as part of integrated weed management in sustainable agriculture. Two hundred fifty-two plant samples from 70 families were collected from Bangladesh and evaluated with the sandwich bioassay. Thirty-one percent of the samples showed significant allelopathic potential on lettuce radicle elongation. Among the species that showed substantial inhibition, more than 7% of the samples showed higher inhibition (HI) and 25% showed moderate inhibition (MI) on lettuce radicle. Fruit pulps of Couroupita guianensis (95.4%), fruits of Phyllanthus emblica (95.4%), and Acacia concinna (95.4%) showed the highest inhibition on lettuce radicle elongation. In contrast, the leaf of Bombax insigne had growth promoting activity by stimulating radicle (23%) and hypocotyl (80%) elongation of lettuce seedlings. This result suggested that the species with significant plant growth inhibitory potential may play a vital role as an alternative to the increasing use of synthetic herbicides for sustainable weed management in agricultural land.
Kohinoor Begum; Mashura Shammi; Nazmul Hasan; Asaduzzaman; Kwame Sarpong Appiah; Yoshiharu Fujii. Potential Allelopathic Candidates for Land Use and Possible Sustainable Weed Management in South Asian Ecosystem. Sustainability 2019, 11, 2649 .
AMA StyleKohinoor Begum, Mashura Shammi, Nazmul Hasan, Asaduzzaman, Kwame Sarpong Appiah, Yoshiharu Fujii. Potential Allelopathic Candidates for Land Use and Possible Sustainable Weed Management in South Asian Ecosystem. Sustainability. 2019; 11 (9):2649.
Chicago/Turabian StyleKohinoor Begum; Mashura Shammi; Nazmul Hasan; Asaduzzaman; Kwame Sarpong Appiah; Yoshiharu Fujii. 2019. "Potential Allelopathic Candidates for Land Use and Possible Sustainable Weed Management in South Asian Ecosystem." Sustainability 11, no. 9: 2649.
Safranal, the main volatile chemical of Saffron (Crocus sativus) was studied to estimate its allelopathic effects on the photosynthetic pigment chlorophyll, leaf electrolyte leakage, fresh weight, catalase (CAT), and peroxidase (POX) activity of the test plant Lettuce (Lactuca sativa). In this study, the effective concentration (EC50) of safranal on CAT was estimated to be 6.12 µg/cm3. CAT activity was inhibited in a dose-dependent manner by the increase in the safranal concentration while POX activity was increased. Moreover, Safranal caused significant physiological changes in chlorophyll content, leaf electrolyte leakage, and fresh weight of several weed species with Lolium multiflorum being the most sensitive. Furthermore, 5 µM Safranal showed significant inhibitory activity against dicotyledonous in comparison to the monocotyledons under greenhouse conditions. The inhibition of the CAT by safranal was similar to those of uncompetitive inhibitors, and therefore the decline in carbon fixation by plants might be the mechanism behind the inhibitory activity of safranal.
Hossein Mardani; John Maninang; Kwame Sarpong Appiah; Yosei Oikawa; Majid Azizi; Yoshiharu Fujii. Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method. Molecules 2019, 24, 1788 .
AMA StyleHossein Mardani, John Maninang, Kwame Sarpong Appiah, Yosei Oikawa, Majid Azizi, Yoshiharu Fujii. Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method. Molecules. 2019; 24 (9):1788.
Chicago/Turabian StyleHossein Mardani; John Maninang; Kwame Sarpong Appiah; Yosei Oikawa; Majid Azizi; Yoshiharu Fujii. 2019. "Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method." Molecules 24, no. 9: 1788.
Background: The in-depth traditional knowledge of medicinal plants is at risk of extinction due to the dependency on oral transmission, and as such, there is an urgent need to document such knowledge. This study aimed to document indigenous uses of medicinal plants among community members in the Ejisu-Juaben Municipality. Methods: Data was collected in 2016 from community members and local herbalists in the Ejisu-Juaben Municipality through a semi-structured questionnaire. Statistical tools and ethnobotanical indices, i.e., informant consensus factor (ICF), fidelity level (FL), and use value (UV) were used to analyse the data. Results: One hundred and six medicinal plants belonging to 45 families were reported to cure 68 different human diseases. The most frequently used plant part in this study was the leaves (52%). Decoction (57.5%) and oral administration (58.3%) were the most utilised herbal preparation and administration route respectively. Cleistopholis patens had the highest UV (0.54) with pain & fevers and skin diseases having the highest ICF values (0.88 and 0.85 respectively). Furthermore, new medicinal uses of Hilleria latifolia and ten other species were recorded for the treatment of the traditional local disease, aseram. Conclusions: The current knowledge and uses of medicinal plants are still high in the study area based on the high degree of consensus among informants. This study could allow for the preservation of knowledge and biodiversity of medicinal plants, both of which are threatened with extinction.
Kwame Sarpong Appiah; Clement Peprah Oppong; Hossein Korrani Mardani; Richard Ansong Omari; Sylvia Kpabitey; Christiana Adukwei Amoatey; Siaw Onwona-Agyeman; Yosei Oikawa; Keisuke Katsura; Yoshiharu Fujii. Medicinal Plants Used in the Ejisu-Juaben Municipality, Southern Ghana: An Ethnobotanical Study. Medicines 2018, 6, 1 .
AMA StyleKwame Sarpong Appiah, Clement Peprah Oppong, Hossein Korrani Mardani, Richard Ansong Omari, Sylvia Kpabitey, Christiana Adukwei Amoatey, Siaw Onwona-Agyeman, Yosei Oikawa, Keisuke Katsura, Yoshiharu Fujii. Medicinal Plants Used in the Ejisu-Juaben Municipality, Southern Ghana: An Ethnobotanical Study. Medicines. 2018; 6 (1):1.
Chicago/Turabian StyleKwame Sarpong Appiah; Clement Peprah Oppong; Hossein Korrani Mardani; Richard Ansong Omari; Sylvia Kpabitey; Christiana Adukwei Amoatey; Siaw Onwona-Agyeman; Yosei Oikawa; Keisuke Katsura; Yoshiharu Fujii. 2018. "Medicinal Plants Used in the Ejisu-Juaben Municipality, Southern Ghana: An Ethnobotanical Study." Medicines 6, no. 1: 1.
Research Highlights: Some organisms such as plants and fungi release certain secondary metabolites, generally called allelochemicals, which can influence the organisms around them. Some of the secondary metabolites released by mushrooms may have certain effects on the growth and development of neighboring plants. Background Objectives: The purpose of the present study was to investigate the allelopathic potential of mushrooms in a forest ecosystem. To this end, 289 Japanese mushroom species were collected from the wild and tested using a modified sandwich method, which is a quick and effective bioassay technique. Materials and Methods: The collected specimens were prepared for bioassay as dried samples, and 10 mg/well (10 cm2) was added to a 6-well multidish according to the mycelia biomass, which was estimated at 700−900 kg ha−1 year−1 (7–9 mg 10 cm−2) in coniferous forests. Results: Of the screened mushroom species, 74% inhibited more than 50% of the radicle elongation in lettuce (Lactuca sativa var. Great Lakes 366) seedlings, while the average of all species was 41.1%. This result suggests that wild mushrooms have a significant regulatory effect on lettuce growth. According to our standard deviation variance analysis, 54 out of 289 species showed significant allelopathic activity. Among these species, Xeromphalina tenuipes, Cortinarius violaceus, and Clavaria miyabeana exhibited the strongest growth inhibitory activity, with radicle elongation of 5.1%, 4.3%, and 7.6% of the control, respectively. In contrast, Ischnoderma resinosum stimulated the length of radicle and hypocotyl growth by 30.6% and 42.0%, respectively. These results suggest that these species may play important roles in ecosystems. In addition, the wide range of allelopathic activities observed in mushrooms indicates that various amounts of diverse secondary metabolites from these species are involved in mushroom allelopathy. Conclusions: Our study reveals the importance of evaluating mushroom allelopathy to understand the wider ecological structures within complex ecosystems.
Asma Osivand; Hiroshi Araya; Kwame S. Appiah; Hossein Mardani; Takayuki Ishizaki; Yoshiharu Fujii. Allelopathy of Wild Mushrooms—An Important Factor for Assessing Forest Ecosystems in Japan. Forests 2018, 9, 773 .
AMA StyleAsma Osivand, Hiroshi Araya, Kwame S. Appiah, Hossein Mardani, Takayuki Ishizaki, Yoshiharu Fujii. Allelopathy of Wild Mushrooms—An Important Factor for Assessing Forest Ecosystems in Japan. Forests. 2018; 9 (12):773.
Chicago/Turabian StyleAsma Osivand; Hiroshi Araya; Kwame S. Appiah; Hossein Mardani; Takayuki Ishizaki; Yoshiharu Fujii. 2018. "Allelopathy of Wild Mushrooms—An Important Factor for Assessing Forest Ecosystems in Japan." Forests 9, no. 12: 773.
Weeds are rapidly developing resistance to synthetic herbicides, and this can pose a threat to the ecosystem. Exploring allelopathic species as an alternative weed control measure can help minimize the ecological threat posed by herbicide-resistant weeds. In this study, we aimed to evaluate the contribution of some polyphenols to the allelopathy of rosemary (Rosmarinus officinalis L.). The phytotoxic effects of rosemary (leaves, roots, inflorescences, and stems) crude extracts were tested on lettuce (Lactuca sativa L.). Soils incorporated with dried rosemary leaves were also tested on test plants. Reversed-phase high-performance liquid chromatography (HPLC) analysis was used to determine the content of some polyphenols (caffeic, ferulic, gallic, rosmarinic, carnosic, and chlorogenic acids) in rosemary. The specific activity and total activity of crude extracts and individual compounds were evaluated using lettuce. The crude extract of rosemary leaves showed the highest growth inhibitory effect among the rosemary organs tested. Soil amended with rosemary leaf debris reduced the dry matter and seed emergence of lettuce. Carnosic acid was the main compound detected in rosemary leaves and had a high specific activity when tested on lettuce. During the seed germination period, there was observed filter paper coloration among the test plants treated with carnosic acid (250 μg/mL). The high concentration and strong inhibitory effect of carnosic acid could explain the inhibitory activity of the rosemary leaf extract. Hence, we conclude based on the total activity estimation that carnosic acid among the other tested compounds is the major allelochemical in rosemary leaves.
Kwame Sarpong Appiah; Hossein Korrani Mardani; Richard Ansong Omari; Vincent Yao Eziah; John Ofosu-Anim; Siaw Onwona-Agyeman; Christiana Adukwei Amoatey; Kiyokazu Kawada; Keisuke Katsura; Yosei Oikawa; Yoshiharu Fujii. Involvement of Carnosic Acid in the Phytotoxicity of Rosmarinus officinalis Leaves. Toxins 2018, 10, 498 .
AMA StyleKwame Sarpong Appiah, Hossein Korrani Mardani, Richard Ansong Omari, Vincent Yao Eziah, John Ofosu-Anim, Siaw Onwona-Agyeman, Christiana Adukwei Amoatey, Kiyokazu Kawada, Keisuke Katsura, Yosei Oikawa, Yoshiharu Fujii. Involvement of Carnosic Acid in the Phytotoxicity of Rosmarinus officinalis Leaves. Toxins. 2018; 10 (12):498.
Chicago/Turabian StyleKwame Sarpong Appiah; Hossein Korrani Mardani; Richard Ansong Omari; Vincent Yao Eziah; John Ofosu-Anim; Siaw Onwona-Agyeman; Christiana Adukwei Amoatey; Kiyokazu Kawada; Keisuke Katsura; Yosei Oikawa; Yoshiharu Fujii. 2018. "Involvement of Carnosic Acid in the Phytotoxicity of Rosmarinus officinalis Leaves." Toxins 10, no. 12: 498.
The use of location-specific and underutilized organic residues (OR) as soil amendments in small-holder agro-ecosystems is promising. Six ORs (Leucaena leucocephala, Centrosema pubescens, Gliricidia sepium, Pueraria phaseoloides, Azadirachta indica, and Theobroma cacao) were amended to three tropical soils each at 24 mg g−1 dry soil in 120-day incubation study to estimate their nitrogen (N) mineralization and microbial biomass carbon (C) dynamics. Inorganic N contents varied among ORs, soil type and incubation days. Regardless of soil type, Gliricidia had the highest inorganic N among the studied ORs. Mineralization rate of 1.4 to 1.5 mg N kg−1 soil day−1 was observed for Lego and Tec soils, respectively, and was twice higher than Nya soil. However, Nya soil released higher inorganic N than Tec and Lego soils, implying high N mineralization efficiency in the former. Consistent soil pH increase was respectively observed for Theobroma and Pueraria treatments in all soils. Moreover, Theobroma and Pueraria amendments showed the highest soil microbial biomass C (MBC) at the end of the incubation. The assessed soil properties likely affected by the dominant edaphic factors and management influenced differences in MBC and dissolved organic carbon (DOC) while OR quality indices controlled N mineralization. Thus, we conclude that soil properties and OR type are important factors for optimal utilization of organic resources.
Richard Ansong Omari; Dorothea Sonoko Bellingrath-Kimura; Yoshiharu Fujii; Elsie Sarkodee-Addo; Kwame Appiah Sarpong; Yosei Oikawa. Nitrogen Mineralization and Microbial Biomass Dynamics in Different Tropical Soils Amended with Contrasting Organic Resources. Soil Systems 2018, 2, 63 .
AMA StyleRichard Ansong Omari, Dorothea Sonoko Bellingrath-Kimura, Yoshiharu Fujii, Elsie Sarkodee-Addo, Kwame Appiah Sarpong, Yosei Oikawa. Nitrogen Mineralization and Microbial Biomass Dynamics in Different Tropical Soils Amended with Contrasting Organic Resources. Soil Systems. 2018; 2 (4):63.
Chicago/Turabian StyleRichard Ansong Omari; Dorothea Sonoko Bellingrath-Kimura; Yoshiharu Fujii; Elsie Sarkodee-Addo; Kwame Appiah Sarpong; Yosei Oikawa. 2018. "Nitrogen Mineralization and Microbial Biomass Dynamics in Different Tropical Soils Amended with Contrasting Organic Resources." Soil Systems 2, no. 4: 63.
This paper presents the first application of ethnobotanical studies to screen for allelopathic species among medicinal plants for sustainable weed management. This study assesses the possible relationship between ethnobotanical indices and allelopathy of medicinal plants. Ethnobotanical data were collected in 2016 by using semi-structured interviews with 140 informants in the Ejisu-Juaben Municipality, Ghana. Data were analysed using statistical tool and ethnobotanical indices including use value (UV), Fidelity Level (FL), Relative Frequency of Citation (RFC). The Sandwich and Dish pack methods were respectively used to evaluate allelopathy through leachates and volatiles of collected samples. Ninety-five species belonging to 43 families are reported in this study, with leaves (52%) cited the most utilised plant part. Cleistopholis patens (UV = 0.54; FL = 90.7%; RFC = 0.37) and Ocimum gratissimum (UV = 0.37; FL = 38.4%; RFC = 0.35) were among the most cited species. Thirty-two species showed inhibition (≥49.3%) by leachates, while twenty-four species were found with potential volatile inhibitory compounds against lettuce radicle growth. There was a significant positive correlation (Pearson) between the UV and RFC of medicinal plants and allelopathy by leaf leachates (r = 0.639 **; p = 0.01 and r = 0.653 **; p = 0.01 respectively). This systematic documentation of medicinal plants in Ejisu-Juaben Municipality shows medicinal plants with ethnomedicinal values and potential allelopathy that can be utilised in sustainable weed control.
Kwame Sarpong Appiah; Hossein Korrani Mardani; Asma Osivand; Sylvia Kpabitey; Christiana Adukwei Amoatey; Yosei Oikawa; Yoshiharu Fujii. Exploring Alternative Use of Medicinal Plants for Sustainable Weed Management. Sustainability 2017, 9, 1468 .
AMA StyleKwame Sarpong Appiah, Hossein Korrani Mardani, Asma Osivand, Sylvia Kpabitey, Christiana Adukwei Amoatey, Yosei Oikawa, Yoshiharu Fujii. Exploring Alternative Use of Medicinal Plants for Sustainable Weed Management. Sustainability. 2017; 9 (8):1468.
Chicago/Turabian StyleKwame Sarpong Appiah; Hossein Korrani Mardani; Asma Osivand; Sylvia Kpabitey; Christiana Adukwei Amoatey; Yosei Oikawa; Yoshiharu Fujii. 2017. "Exploring Alternative Use of Medicinal Plants for Sustainable Weed Management." Sustainability 9, no. 8: 1468.