Several studies state that phase change material (PCM) improves the electrical power and efficiency of the photovoltaic (PV) module. To find the suitable PCM for tropical climatic conditions, multi-PCMs are examined simultaneously with melting temperatures of 31 °C, 35 °C, 37 °C, and 42 °C. In this study, PCM containers are integrated behind the PV module with a thickness of 50 mm. The performance of the multi PV-PCMs is monitored year-round and compared with PV-noPCM. The experimental results show that the selected four PCMs performed the cooling process autonomously in all the climates, such as PCM with a melting temperature of 37 °C and 42 °C enhanced the higher cooling rate in summer, and the same PCMs failed to achieve a higher cooling rate in winter. The lowest temperature drop was noted for pre-monsoon and monsoon seasons due to the low irradiance. On the other hand, the highest temperature drop of 16.33 °C is observed for pre-summer (March) and 15.7 °C, and 17.14 °C for summer (April) as compared to PV-noPCM. The results of the present investigation highlight the requirement for choosing the proper PCM melting temperature based on optimal year-round performance. Further, it is recommended that a single PCM melting temperature for cooling the PV modules year-round in tropical climates is inappropriate, and instead, a cascaded structure with different PCM melting temperatures is recommended.

In this study, we report the assembly and annotation of the mitochondrial genome (mitogenome) of Acheta domesticus from breeding facility, a species commonly known as the house cricket. This species is considered to be an important edible cricket. The mitogenome was assembled using a reproducible protocol implemented on the Galaxy Europe Server, which involved uploading paired‐end fastq reads for bioinformatic analysis. The resulting mitogenome is 15,784 base pairs in length and has a GC content of 29.05%. The nucleotide composition of this mitogenome is similar to that of other insect mitogenomes, with A, T, C, and G nucleotides comprising 39.2%, 31.7%, 19.6%, and 9.5% of the mitogenome, respectively. The gene organization of the A. domesticus mitogenome is identical to that of other cricket species. The mitogenome consists of 37 genes, including 13 protein‐coding genes, 22 tRNA genes, and two rRNA genes. The congruence between PCA and Bayesian evolutionary tree analysis in clustering the divergent A. domesticus sequences highlights these genomes as candidates for further study to elucidate their distinct features and evolutionary history.

The red‐whiskered bulbul (Pycnonotus jocosus) is a popular avian species in Thailand and many other countries. The red‐whiskered bulbul has a high economic value, but breeding is challenging since sex identification is difficult. The PCR method is now used for sex identification. However, PCR amplification and post‐PCR analysis necessitate the use of a laboratory equipped with specialized scientific instruments, which is inconvenient for field operations. This research describes a method for amplification of DNA samples using the loop‐mediated isothermal amplification (LAMP) approach, which is a molecular biology methodology for isothermal amplification that is extremely sensitive, fast, and easy for post‐LAMP product visualization. Herein, total of 23 blood samples were collected and DNA was extracted. Two sets of LAMP primers were designed for CHD‐Z and CHD‐W genes. The colorimetric assay was used to investigate the best conditions for LAMP reactions and post‐LAMP product visualization. LAMP reactions for sex identification were compared to traditional PCR in terms of sensitivity and specificity. LAMP reactions were found to be 10‐fold more sensitive than PCR at 1 ng of DNA. When compared to electrophoresis analysis, the visualization with colorimetric assay using GelRed® and SYTO™ 9 was 100% accurate. The optimal LAMP condition tested simple DNA extracted from bird feathers using the HotSHOT technique. The result showed that the optimal condition could distinguish the sex of red‐whiskered bulbuls totally and accurately. A powerful method for red‐whiskered bulbul sex identification is demonstrated in this study, which can be used in field studies because it is quick and easy to perform, has high sensitivity, and does not require advanced scientific equipment.

Herein, we have described genome wide screening of microsatellites (Simple Sequence Repeats: SSRs) and their distribution in the Acheta domesticus genome using a custom python script. A total of 232,179 microsatellites were identified, in which trinucleotide repeats were found to be the most abundant repeats in the genome, representing 60 % of the total microsatellite, followed by dinucleotides, tetranucleotides, pentanucleotides, and hexanucleotides. Among trinucleotides, dinucleotides, and tetranucleotides, the most prevalent microsatellite motifs were AAT/ATT, TG/CA, and AAAT/ATTT, respectively. Notably, statistical analysis of the SSR repetition frequency distribution showed that SSR motifs that were repeated four times were the most recurrent. Additionally, the SSR length distribution showed that SSRs with a12 bp size were the most common. As a result, the statistical analysis of the SSR dataset in A. domesticus will be a useful resource for a better understanding of microsatellite distribution in crickets for evolutionary genetics.