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Prof. Ivica Strelec
Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia

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0 Electrophoresis
0 Food Technology
0 Protein Purification
0 Food Chemistry and Biochemistry
0 Enzyme immobilization

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Journal article
Published: 27 August 2021 in Pharmaceuticals
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Carbon quantum dots (CQDs) have recently emerged as innovative theranostic nanomaterials, enabling fast and effective diagnosis and treatment. In this study, a facile hydrothermal approach for N-doped biomass-derived CQDs preparation from Citrus clementina peel and amino acids glycine (Gly) and arginine (Arg) has been presented. The gradual increase in the N-dopant (amino acids) nitrogen content increased the quantum yield of synthesized CQDs. The prepared CQDs exhibited good biocompatibility, stability in aqueous, and high ionic strength media, similar optical properties, while differences were observed regarding the structural and chemical diversity, and biological and antioxidant activity. The antiproliferative effect of [email protected] against pancreatic cancer cell lines (CFPAC-1) was observed. At the same time, [email protected] has demonstrated the highest quantum yield and antioxidant activity by DPPH scavenging radical method of 81.39 ± 0.39% and has been further used for the ion sensing and cellular imaging of cancer cells. The obtained results have demonstrated selective response toward Fe3+ detection, with linear response ranging from 7.0 µmol dm−3 to 50.0 µmol dm−3 with R2 = 0.9931 and limit of detection (LOD) of 4.57 ± 0.27 µmol dm−3. This research could be a good example of sustainable biomass waste utilization with potential for biomedical analysis and ion sensing applications.

ACS Style

Silvija Šafranko; Anamarija Stanković; Sugato Hajra; Hoe-Joon Kim; Ivica Strelec; Maja Dutour-Sikirić; Igor Weber; Maja Herak Bosnar; Petra Grbčić; Sandra Kraljević Pavelić; Aleksandar Széchenyi; Yogendra Kumar Mishra; Igor Jerković; Stela Jokić. Preparation of Multifunctional N-Doped Carbon Quantum Dots from Citrus clementina Peel: Investigating Targeted Pharmacological Activities and the Potential Application for Fe3+ Sensing. Pharmaceuticals 2021, 14, 857 .

AMA Style

Silvija Šafranko, Anamarija Stanković, Sugato Hajra, Hoe-Joon Kim, Ivica Strelec, Maja Dutour-Sikirić, Igor Weber, Maja Herak Bosnar, Petra Grbčić, Sandra Kraljević Pavelić, Aleksandar Széchenyi, Yogendra Kumar Mishra, Igor Jerković, Stela Jokić. Preparation of Multifunctional N-Doped Carbon Quantum Dots from Citrus clementina Peel: Investigating Targeted Pharmacological Activities and the Potential Application for Fe3+ Sensing. Pharmaceuticals. 2021; 14 (9):857.

Chicago/Turabian Style

Silvija Šafranko; Anamarija Stanković; Sugato Hajra; Hoe-Joon Kim; Ivica Strelec; Maja Dutour-Sikirić; Igor Weber; Maja Herak Bosnar; Petra Grbčić; Sandra Kraljević Pavelić; Aleksandar Széchenyi; Yogendra Kumar Mishra; Igor Jerković; Stela Jokić. 2021. "Preparation of Multifunctional N-Doped Carbon Quantum Dots from Citrus clementina Peel: Investigating Targeted Pharmacological Activities and the Potential Application for Fe3+ Sensing." Pharmaceuticals 14, no. 9: 857.

Review
Published: 25 January 2021 in Biomolecules
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Lipoxygenases are widespread enzymes that catalyze oxidation of polyunsaturated fatty acids (linoleic, linolenic, and arachidonic acid) to produce hydroperoxides. Lipoxygenase reactions can be desirable, but also lipoxygenases can react in undesirable ways. Most of the products of lipoxygenase reactions are aromatic compounds that can affect food properties, especially during long-term storage. Lipoxygenase action on unsaturated fatty acids could result in off-flavor/off-odor development, causing food spoilage. In addition, lipoxygenases are present in the human body and play an important role in stimulation of inflammatory reactions. Inflammation is linked to many diseases, such as cancer, stroke, and cardiovascular and neurodegenerative diseases. This review summarized recent research on plant families and species that can inhibit lipoxygenase activity.

ACS Style

Melita Lončarić; Ivica Strelec; Tihomir Moslavac; Drago Šubarić; Valentina Pavić; Maja Molnar. Lipoxygenase Inhibition by Plant Extracts. Biomolecules 2021, 11, 152 .

AMA Style

Melita Lončarić, Ivica Strelec, Tihomir Moslavac, Drago Šubarić, Valentina Pavić, Maja Molnar. Lipoxygenase Inhibition by Plant Extracts. Biomolecules. 2021; 11 (2):152.

Chicago/Turabian Style

Melita Lončarić; Ivica Strelec; Tihomir Moslavac; Drago Šubarić; Valentina Pavić; Maja Molnar. 2021. "Lipoxygenase Inhibition by Plant Extracts." Biomolecules 11, no. 2: 152.

Journal article
Published: 17 July 2020 in Pharmaceuticals
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Lipoxygenases (LOXs) are a family of enzymes found in plants, mammals, and microorganisms. In animals and plants, the enzyme has the capability for the peroxidation of unsaturated fatty acids. Although LOXs participate in the plant defense system, the enzyme’s metabolites can have numerous negative effects on human health. Therefore, many types of research are searching for compounds that can inhibit LOXs. The best quantitative structure–activity relationship (QSAR) model was obtained using a Genetic Algorithm (GA). Molecular docking was performed with iGEMDOCK. The inhibition of lipoxygenase was in the range of 7.1 to 96.6%, and the inhibition of lipid peroxidation was 7.0–91.0%. Among the synthesized compounds, the strongest inhibitor of soybean LOX-3 (96.6%) was found to be 3-benzoyl-7-(benzyloxy)-2H-chromen-2-one. A lipid peroxidation inhibition of 91.0% was achieved with ethyl 7-methoxy-2-oxo-2H-chromene-3-carboxylate. The docking scores for the soybean LOX-3 and human 5-LOX also indicated that this compound has the best affinity for these LOX enzymes. The best multiple linear QSAR model contains the atom-centered fragment descriptors C-06, RDF035p, and HATS8p. QSAR and molecular docking studies elucidated the structural features important for the enhanced inhibitory activity of the most active compounds, such as the presence of the benzoyl ring at the 3-position of coumarin’s core. Compounds with benzoyl substituents are promising candidates as potent lipoxygenase inhibitors.

ACS Style

Melita Lončarić; Ivica Strelec; Valentina Pavić; Domagoj Šubarić; Vesna Rastija; Maja Molnar. Lipoxygenase Inhibition Activity of Coumarin Derivatives—QSAR and Molecular Docking Study. Pharmaceuticals 2020, 13, 154 .

AMA Style

Melita Lončarić, Ivica Strelec, Valentina Pavić, Domagoj Šubarić, Vesna Rastija, Maja Molnar. Lipoxygenase Inhibition Activity of Coumarin Derivatives—QSAR and Molecular Docking Study. Pharmaceuticals. 2020; 13 (7):154.

Chicago/Turabian Style

Melita Lončarić; Ivica Strelec; Valentina Pavić; Domagoj Šubarić; Vesna Rastija; Maja Molnar. 2020. "Lipoxygenase Inhibition Activity of Coumarin Derivatives—QSAR and Molecular Docking Study." Pharmaceuticals 13, no. 7: 154.

Journal article
Published: 20 January 2020 in Scientific Reports
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The impact of fullerene C60 water soluble daughter molecules - fullerols C60(OH)24 nanoparticles (FNP) on emerging (non-aflatoxin biosynthetic pathway) toxins production in mycelia and yeast extract sucrose (YES) media of A. flavus was investigated under growth conditions of 29 °C in the dark for a 168 h period. The FNP solution (10, 100 and 1000 ng mL−1) contained predominantly nanoparticles of 8 nm diameter and with zeta potential mean value of −33 mV. Ten emerging metabolites were produced at concentrations reaching 1,745,035 ng 50 mL−1 YES medium. Seven of the metabolites were found in mycelia and media, while three were only in mycelia. Majority of the metabolites were detected in higher quantity in mycelia than in media, at a ratio of 99:1 (m/m). However, higher metabolite quantities were found in media following FNP application, while FNP caused a decrease of total metabolite quantities in mycelia. The concentrations of the metabolites in media increased in the presence of 1000 ng mL−1 FNP while mycelial quantities of the metabolites decreased with increased applied FNP dose. The impacts of global climate changes on FNP availability in the environment and on mycotoxin occurrence in crops increase the relevance of this study for risk assessment of nanoparticles. Cordycepin is reported for the first time as metabolite of A. flavus.

ACS Style

Tihomir Kovač; Ivana Borišev; Marija Kovač; Ante Lončarić; Frane Čačić Kenjerić; Aleksandar Djordjevic; Ivica Strelec; Chibundu N. Ezekiel; Michael Sulyok; Rudolf Krska; Bojan Šarkanj. Impact of fullerol C60(OH)24 nanoparticles on the production of emerging toxins by Aspergillus flavus. Scientific Reports 2020, 10, 725 -10.

AMA Style

Tihomir Kovač, Ivana Borišev, Marija Kovač, Ante Lončarić, Frane Čačić Kenjerić, Aleksandar Djordjevic, Ivica Strelec, Chibundu N. Ezekiel, Michael Sulyok, Rudolf Krska, Bojan Šarkanj. Impact of fullerol C60(OH)24 nanoparticles on the production of emerging toxins by Aspergillus flavus. Scientific Reports. 2020; 10 (1):725-10.

Chicago/Turabian Style

Tihomir Kovač; Ivana Borišev; Marija Kovač; Ante Lončarić; Frane Čačić Kenjerić; Aleksandar Djordjevic; Ivica Strelec; Chibundu N. Ezekiel; Michael Sulyok; Rudolf Krska; Bojan Šarkanj. 2020. "Impact of fullerol C60(OH)24 nanoparticles on the production of emerging toxins by Aspergillus flavus." Scientific Reports 10, no. 1: 725-10.

Journal article
Published: 25 April 2019 in Applied Sciences
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A series of fluorinated 4,5-dihydro-1H-pyrazole derivatives were synthesized in the reaction of corresponding acetophenone and different aldehydes followed by the second step synthesis of desired compounds from synthesized chalcone, hydrazine hydrate, and formic acid. Structures of all compounds were confirmed by both 1H and 13C NMR and mass spectrometry. Antibacterial properties of compounds were tested on four bacterial strains, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus. Among synthesized compounds, the strongest inhibitor of monophenolase activity of mushroom tyrosinase (32.07 ± 3.39%) was found to be 5-(2-chlorophenyl)-3-(4-fluorophenyl)-4,5-dihydro-1H-pyrazole-1-carbaldehyde. The PASS program has predicted the highest probable activity for the phosphodiesterase inhibition. To shed light on molecular interactions between the synthesized compounds and phosphodiesterase, all compounds were docked into the active binding site. The obtained results showed that the compound with the dimethoxyphenyl ring could be potent as an inhibitor of phosphodiesterase, which interacts in PDE5 catalytic domain of the enzyme. Key interactions are bidentate hydrogen bond (H-bond) with the side-chain of Gln817 and van der Waals interactions of the dimethoxyphenyl ring and pyrazole ring with hydrophobic clamp, which contains residuals, Val782, Phe820, and Tyr612. Interactions are similar to the binding mode of the inhibitor sildenafil, the first oral medicine for the treatment of male erectile dysfunction.

ACS Style

Vesna Rastija; Harshad Brahmbhatt; Maja Molnar; Melita Lončarić; Ivica Strelec; Mario Komar; Valentina Pavić. Synthesis, Tyrosinase Inhibiting Activity and Molecular Docking of Fluorinated Pyrazole Aldehydes as Phosphodiesterase Inhibitors. Applied Sciences 2019, 9, 1704 .

AMA Style

Vesna Rastija, Harshad Brahmbhatt, Maja Molnar, Melita Lončarić, Ivica Strelec, Mario Komar, Valentina Pavić. Synthesis, Tyrosinase Inhibiting Activity and Molecular Docking of Fluorinated Pyrazole Aldehydes as Phosphodiesterase Inhibitors. Applied Sciences. 2019; 9 (8):1704.

Chicago/Turabian Style

Vesna Rastija; Harshad Brahmbhatt; Maja Molnar; Melita Lončarić; Ivica Strelec; Mario Komar; Valentina Pavić. 2019. "Synthesis, Tyrosinase Inhibiting Activity and Molecular Docking of Fluorinated Pyrazole Aldehydes as Phosphodiesterase Inhibitors." Applied Sciences 9, no. 8: 1704.

Journal article
Published: 10 December 2018 in Toxins
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Aspergillus flavus is the most important mycotoxin-producing fungus involved in the global episodes of aflatoxin B1 contamination of crops at both the pre-harvest and post-harvest stages. However, in order to effectively control aflatoxin contamination in crops using antiaflatoxigenic and/or antifungal compounds, some of which are photosensitive, a proper understanding of the photo-sensitive physiology of potential experimental strains need to be documented. The purpose of the study is therefore to evaluate the effect of visible (VIS) light illumination on growth and conidiation, aflatoxin production ability and modulation of A. flavus oxidative status during in vitro experiment. Aflatoxigenic A. flavus strain was inoculated in aflatoxin-inducing YES media and incubated under three different VIS illumination regimes during a 168 h growth period at 29 °C. VIS illumination reduced A. flavus mycelia biomass yield, both during growth on plates and in liquid media, promoted conidiation and increased the aflatoxin production. Furthermore, aflatoxin production increased with increased reactive oxidative species (ROS) levels at 96 h of growth, confirming illumination-driven oxidative stress modulation activity on A. flavus cells.

ACS Style

Tihomir Kovač; Bojan Šarkanj; Biljana Crevar; Marija Kovač; Ante Lončarić; Ivica Strelec; Chibundu N. Ezekiel; Michael Sulyok; Rudolf Krska. Aspergillus flavus NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability In Vitro under Different Illumination Regimes. Toxins 2018, 10, 528 .

AMA Style

Tihomir Kovač, Bojan Šarkanj, Biljana Crevar, Marija Kovač, Ante Lončarić, Ivica Strelec, Chibundu N. Ezekiel, Michael Sulyok, Rudolf Krska. Aspergillus flavus NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability In Vitro under Different Illumination Regimes. Toxins. 2018; 10 (12):528.

Chicago/Turabian Style

Tihomir Kovač; Bojan Šarkanj; Biljana Crevar; Marija Kovač; Ante Lončarić; Ivica Strelec; Chibundu N. Ezekiel; Michael Sulyok; Rudolf Krska. 2018. "Aspergillus flavus NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability In Vitro under Different Illumination Regimes." Toxins 10, no. 12: 528.

Research article
Published: 08 October 2018 in Engineering in Life Sciences
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In order to increase the current knowledge on cold‐press oil cakes composition, the present study aims to determine the chemical composition of oil cakes from hull‐less pumpkin (Cucurbita pepo L.), flax (Linum usitatissimum L.), and hemp (Canabis sativa L.) before and after the biological treatment with Trametes versicolor and Humicola grisea using fungal‐based solid‐state technology. After 10 days of treatment, the content of ash, total nitrogen, total proteins, and total organic carbon increased in all three oil cakes, while the content of ether extracts decreased. After treatment, the concentration of soluble carbohydrates decreased in pumpkin and hemp seed oil cakes, whereas it increased in flaxseed oil cake. During treatment with T. versicolor, the content of fructose significantly increased in hull‐less pumpkin seed oil cake. Fiber content decreased in pumpkin and flaxseed oil cakes after treatment with both of the fungi, whereas it increased in flaxseed oil cake. This article is protected by copyright. All rights reserved

ACS Style

Sandra Budžaki; Ivica Strelec; Mija Krnić; Kristina Alilović; Marina Tišma; Bruno Zelić. Proximate analysis of cold-press oil cakes after biological treatment with Trametes versicolor and Humicola grisea. Engineering in Life Sciences 2018, 18, 924 -931.

AMA Style

Sandra Budžaki, Ivica Strelec, Mija Krnić, Kristina Alilović, Marina Tišma, Bruno Zelić. Proximate analysis of cold-press oil cakes after biological treatment with Trametes versicolor and Humicola grisea. Engineering in Life Sciences. 2018; 18 (12):924-931.

Chicago/Turabian Style

Sandra Budžaki; Ivica Strelec; Mija Krnić; Kristina Alilović; Marina Tišma; Bruno Zelić. 2018. "Proximate analysis of cold-press oil cakes after biological treatment with Trametes versicolor and Humicola grisea." Engineering in Life Sciences 18, no. 12: 924-931.

Journal article
Published: 27 August 2018 in Scientific Reports
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The water soluble fullerene C60 daughter product - fullerols C60(OH)24 (FNP) possesses a great potential of modifying secondary metabolites biosynthesis. In order to clarify the extent of interaction, the impact of FNP (10, 100 and 1000 ng mL−1) on aflatoxin production and the available precursors of biosynthesis pathway from Aspergillus flavus NRRL 3251 was determined, in both the mycelia and yeast extract sucrose (YES) medium, during a 168-hour growth period at 29 °C in the dark. The FNP of 8 nm in diameter, and with a zeta potential of −33 mV affected mycelial growth at 1000 ng mL−1 while conidia production was slightly affected at 10 ng mL−1. The FNP effect on aflatoxin and it biosynthetic precursors was concentration dependent and alteration of the sterigmatocystin (ST) export from the cell was observed. Most of the monitored aflatoxin precursors, except norsolorinic acid, were detected in both mycelia and YES medium. However, observed precursor concentrations were much higher in mycelia, with exception of ST. The study shows the loss of FNP antioxidative effect after 120 hours of growth, and strong concentration dependent aflatoxigenic effect after that time. Thus, this data is relevant to guide future considerations on FNP-fungal interactions in the environments and on risk assessment.

ACS Style

Tihomir Kovač; Ivana Borišev; Biljana Crevar; Frane Čačić Kenjerić; Marija Kovač; Ivica Strelec; Chibundu N. Ezekiel; Michael Sulyok; Rudolf Krska; Bojan Šarkanj. Fullerol C60(OH)24 nanoparticles modulate aflatoxin B1 biosynthesis in Aspergillus flavus. Scientific Reports 2018, 8, 1 -8.

AMA Style

Tihomir Kovač, Ivana Borišev, Biljana Crevar, Frane Čačić Kenjerić, Marija Kovač, Ivica Strelec, Chibundu N. Ezekiel, Michael Sulyok, Rudolf Krska, Bojan Šarkanj. Fullerol C60(OH)24 nanoparticles modulate aflatoxin B1 biosynthesis in Aspergillus flavus. Scientific Reports. 2018; 8 (1):1-8.

Chicago/Turabian Style

Tihomir Kovač; Ivana Borišev; Biljana Crevar; Frane Čačić Kenjerić; Marija Kovač; Ivica Strelec; Chibundu N. Ezekiel; Michael Sulyok; Rudolf Krska; Bojan Šarkanj. 2018. "Fullerol C60(OH)24 nanoparticles modulate aflatoxin B1 biosynthesis in Aspergillus flavus." Scientific Reports 8, no. 1: 1-8.

Journal article
Published: 25 May 2018 in Croatian journal of food science and technology
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ACS Style

Ivica Strelec; Biljana Crevar; Tihomir Kovac; Blanka Bilic Rajs; Ljiljana Primorac; Ivana Flanjak. Glucose oxidase activity and hydrogen peroxide accumulation in Croatian honeys. Croatian journal of food science and technology 2018, 10, 33 -41.

AMA Style

Ivica Strelec, Biljana Crevar, Tihomir Kovac, Blanka Bilic Rajs, Ljiljana Primorac, Ivana Flanjak. Glucose oxidase activity and hydrogen peroxide accumulation in Croatian honeys. Croatian journal of food science and technology. 2018; 10 (1):33-41.

Chicago/Turabian Style

Ivica Strelec; Biljana Crevar; Tihomir Kovac; Blanka Bilic Rajs; Ljiljana Primorac; Ivana Flanjak. 2018. "Glucose oxidase activity and hydrogen peroxide accumulation in Croatian honeys." Croatian journal of food science and technology 10, no. 1: 33-41.

Journal article
Published: 05 February 2018 in AMB Express
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Increased interest in fullerene C60 and derivatives in recent years implies an intensification of their environmental spread. Yet, the potential risks for living organisms are largely unknown, including the interaction of C60 with fungal organisms. This may be especially relevant for mycotoxigenic fungi since C60 may both scavenge and produce reactive oxygen species (ROS), and oxidative stress induces mycotoxin production in fungi. Therefore, this study examined effects of environmentally plausible concentrations of C60 (0, 10, 50, and 100 ng/mL) on Aspergillus flavus growth and aflatoxin production in culture media. In addition, ROS-dependent oxidative stress biomarkers—thiobarbituric acid reactive substances (TBARS), reduced and oxidised glutathione ratio, superoxide dismutase isoenzymes, catalase, glutathione peroxidase, and glutathione reductase were determined in mycelia. Nanoparticles of fullerene C60 (nC60) did not exhibit strong antifungal activity against A. flavus. At the same time, nC60 caused an antiaflatoxigenic effect at 10–100 ng/mL, and 50 ng/mL unexpectedly enhanced aflatoxin production. The TBARS content, reduced and oxidised glutathione ratio, and copper, zinc superoxide dismutase activity suggest that 10 ng/mL nC60 exerted antioxidative action and reduced aflatoxin B1 production within fungal cells. Detected prooxidative effects of 50 ng/mL fullerene exceeded cellular defenses and consequently enhanced aflatoxin B1 production. Finally, the results obtained with 100 ng/mL nC60 point to prooxidative effects, but the absence of increase in aflatoxin output may indicate additional, presumably cytotoxic effects of nC60. Thus, a range of rather low levels of nC60 in the environment has a potential to modify aflatoxin production in A. flavus. Due to possible implications, further studies should test these results in environmental conditions.

ACS Style

Tihomir Kovač; Bojan Šarkanj; Tomislav Klapec; Ivana Borišev; Marija Kovač; Ante Nevistić; Ivica Strelec. Antiaflatoxigenic effect of fullerene C60 nanoparticles at environmentally plausible concentrations. AMB Express 2018, 8, 1 -8.

AMA Style

Tihomir Kovač, Bojan Šarkanj, Tomislav Klapec, Ivana Borišev, Marija Kovač, Ante Nevistić, Ivica Strelec. Antiaflatoxigenic effect of fullerene C60 nanoparticles at environmentally plausible concentrations. AMB Express. 2018; 8 (1):1-8.

Chicago/Turabian Style

Tihomir Kovač; Bojan Šarkanj; Tomislav Klapec; Ivana Borišev; Marija Kovač; Ante Nevistić; Ivica Strelec. 2018. "Antiaflatoxigenic effect of fullerene C60 nanoparticles at environmentally plausible concentrations." AMB Express 8, no. 1: 1-8.

Correction
Published: 17 November 2017 in Food Analytical Methods
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The original version of this article unfortunately contained an incorrect version of author’s names and surnames (underlined). The correct names and surnames are: “Ivica Strelec”, “Lidija Brodar”, “Iv

ACS Style

Ivica Strelec; Lidija Brodar; Ivana Flanjak; Frane Čačić Kenjerić; Tihomir Kovač; Daniela Čačić Kenjerić; Ljiljana Primorac. Correction to: Characterization of Croatian Honeys by Right-Angle Fluorescence Spectroscopy and Chemometrics. Food Analytical Methods 2017, 11, 839 -839.

AMA Style

Ivica Strelec, Lidija Brodar, Ivana Flanjak, Frane Čačić Kenjerić, Tihomir Kovač, Daniela Čačić Kenjerić, Ljiljana Primorac. Correction to: Characterization of Croatian Honeys by Right-Angle Fluorescence Spectroscopy and Chemometrics. Food Analytical Methods. 2017; 11 (3):839-839.

Chicago/Turabian Style

Ivica Strelec; Lidija Brodar; Ivana Flanjak; Frane Čačić Kenjerić; Tihomir Kovač; Daniela Čačić Kenjerić; Ljiljana Primorac. 2017. "Correction to: Characterization of Croatian Honeys by Right-Angle Fluorescence Spectroscopy and Chemometrics." Food Analytical Methods 11, no. 3: 839-839.

Article
Published: 14 October 2017 in Food Analytical Methods
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The potential of right-angle fluorescence spectroscopy and selected chemical parameters for discrimination of botanical origin of Croatian honey types (n = 55) previously classified by physicochemical and melissopalynological analyses was evaluated. Systematic step-by-step fluorescence analysis included the measurement of complete excitation-emission matrix (EEM) in the range of excitation wavelengths from 260 to 400 nm, and emission wavelengths from 300 to 600 nm, followed by fluorescence intensity measurement at detected peaks of different excitation/emission wavelengths, and emission spectra recordings at selected specific excitation wavelengths of honey solutions in 50 mmol L−1 phosphate buffer pH 7.0 and methanol. A total of five different sets of emission spectral data for buffer and two for methanolic honey solutions were considered for chemometric analysis of original and normalized emission spectra including principal component analysis and linear discriminant analysis. Additionally, chemical analysis of buffer and methanolic honey solutions included determination of protein, total polyphenol and reactive amino group content. Results showed that right-angle fluorescence spectroscopy of honey solutions has great potential for honey botanical origin discrimination, either by visual comparison of excitation-emission spectra landscapes, or even better, by normalized emission fluorescence spectra recordings at 260 and 280 nm of excitation. Moreover, increased honey discrimination was achieved in conjunction with chemometrics of fluorescence spectras. In addition, two rapid markers/indicators of honey authentication were found. Chestnut honey could be clearly discriminated from the other honey types by simple measurement of fluorescence intensity at 390/470 nm, while sage honeys by measurement of reactive amino group content.

ACS Style

Ivica Strelec; Lidija Brodar; Ivana Flanjak; Frane Čačić Kenjerić; Tihomir Kovač; Daniela Čačić Kenjerić; Ljiljana Primorac. Characterization of Croatian Honeys by Right-Angle Fluorescence Spectroscopy and Chemometrics. Food Analytical Methods 2017, 11, 824 -838.

AMA Style

Ivica Strelec, Lidija Brodar, Ivana Flanjak, Frane Čačić Kenjerić, Tihomir Kovač, Daniela Čačić Kenjerić, Ljiljana Primorac. Characterization of Croatian Honeys by Right-Angle Fluorescence Spectroscopy and Chemometrics. Food Analytical Methods. 2017; 11 (3):824-838.

Chicago/Turabian Style

Ivica Strelec; Lidija Brodar; Ivana Flanjak; Frane Čačić Kenjerić; Tihomir Kovač; Daniela Čačić Kenjerić; Ljiljana Primorac. 2017. "Characterization of Croatian Honeys by Right-Angle Fluorescence Spectroscopy and Chemometrics." Food Analytical Methods 11, no. 3: 824-838.

Research article
Published: 30 May 2017 in Environmental Science and Pollution Research
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Increased use of fullerols in various fields and expected increase of their environmental spread impose the necessity for testing fullerol nanoparticles (FNP) effects on microbiota. There is little information available on the interaction of mycotoxigenic fungi and FNP, despite FNP having a great potential of modifying mycotoxin production. Namely, FNP exhibit both ROS-quenching and ROS-producing properties, while oxidative stress stimulates mycotoxin synthesis in the fungi. In order to shed some light on the extent of interaction between FNP and mycotoxigenic fungi, the effects of fullerol C60(OH)24 nanoparticles (10, 100, 1000 ng/mL) on mycelial growth, aflatoxin production and oxidative stress modulation in an aflatoxigenic strain of Aspergillus flavus (NRRL 3251) during 168 h of incubation in a liquid culture medium were examined. FNP slightly reduced mycelial biomass weight, but significantly decreased aflatoxin concentration in media. Lipid peroxide content, superoxide dismutase, catalase and glutathione peroxidase activities suggest that FNP treatments hormetically reduced oxidative stress within fungal cells in turn suppressing aflatoxin production. These findings contribute to the assessment of environmental risk and application potential of fullerols.

ACS Style

Tihomir Kovač; Bojan Šarkanj; Tomislav Klapec; Ivana Borišev; Marija Kovač; Ante Nevistić; Ivica Strelec. Fullerol C60(OH)24 nanoparticles and mycotoxigenic fungi: a preliminary investigation into modulation of mycotoxin production. Environmental Science and Pollution Research 2017, 24, 16673 -16681.

AMA Style

Tihomir Kovač, Bojan Šarkanj, Tomislav Klapec, Ivana Borišev, Marija Kovač, Ante Nevistić, Ivica Strelec. Fullerol C60(OH)24 nanoparticles and mycotoxigenic fungi: a preliminary investigation into modulation of mycotoxin production. Environmental Science and Pollution Research. 2017; 24 (20):16673-16681.

Chicago/Turabian Style

Tihomir Kovač; Bojan Šarkanj; Tomislav Klapec; Ivana Borišev; Marija Kovač; Ante Nevistić; Ivica Strelec. 2017. "Fullerol C60(OH)24 nanoparticles and mycotoxigenic fungi: a preliminary investigation into modulation of mycotoxin production." Environmental Science and Pollution Research 24, no. 20: 16673-16681.

Journal article
Published: 01 March 2017 in Archives of Industrial Hygiene and Toxicology
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The antifungal and antiaflatoxigenic effects of two series of coumarinyl thiosemicarbazides on Aspergillus flavus NRRL 3251 were studied. Fungi were grown in YES medium for 72 h at 29 °C in the presence of 0, 0.1, 1, and 10 μg mL-1 of coumarinyl thiosemicarbazides: one series with substitution in position 7 and another with substitution in position 4 of the coumarin core. Dry mycelia weight determination was used for antifungal activity estimation, while the aflatoxin B1 content in YES media, determined by the dilute and shoot LC-MS/MS technique, was used for the antiaflatoxigenic effect estimation. Standard biochemical assays were used for oxidative status marker (TBARS, SOD, CAT, and GPX) determination in A. flavus NRRL 3251 mycelia. Results show that 7-substituted-coumarinyl thiosemicarbazides possess a better antifungal and antiaflatoxigenic activity than 4-substituted ones. The most prominent substituted compound was the compound 3, N-(4-chlorophenyl)-2-(2-((4-methyl-2-oxo-2H-chromen-7-yl)oxy)acetyl)hydrazine-1-carbothioamide, which completely inhibited aflatoxin production at the concentration of 10 μg mL-1. Oxidative stress response of A. flavus exposed to the selected compounds points to the modulation of oxidative stress as a possible reason of aflatoxin production inhibition.

ACS Style

Tihomir Kovač; Marija Kovač; Ivica Strelec; Ante Nevistić; Maja Molnar. Antifungal and antiaflatoxigenic activities of coumarinyl thiosemicarbazides against Aspergillus flavus NRRL 3251. Archives of Industrial Hygiene and Toxicology 2017, 68, 9 -15.

AMA Style

Tihomir Kovač, Marija Kovač, Ivica Strelec, Ante Nevistić, Maja Molnar. Antifungal and antiaflatoxigenic activities of coumarinyl thiosemicarbazides against Aspergillus flavus NRRL 3251. Archives of Industrial Hygiene and Toxicology. 2017; 68 (1):9-15.

Chicago/Turabian Style

Tihomir Kovač; Marija Kovač; Ivica Strelec; Ante Nevistić; Maja Molnar. 2017. "Antifungal and antiaflatoxigenic activities of coumarinyl thiosemicarbazides against Aspergillus flavus NRRL 3251." Archives of Industrial Hygiene and Toxicology 68, no. 1: 9-15.

Journal article
Published: 01 June 2016 in Journal of Apicultural Science
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In honey, the content of proteins, including the enzymes, is relatively low and has a minor nutritive significance. On the other hand, the proteins, including the enzymes, are usually used as honey quality evaluation parameters. This is because protein content and enzyme activities vary regarding the botanical origin of the honey. Since the results of protein content, glucose-oxidase, and acid phosphatase, for honeys produced in Croatia, are not available, four of the most abundant honey types produced in Croatia (black locust, sage, chestnut, and honeydew honey) are characterised according to the protein and proline content and enzyme activities. The characterisation was done to determine specificities and contribute to the characterisation of unifloral honeys. Dark honey types (honeydew and chestnut honey) had a higher proline content, and diastase, invertase, and glucose-oxidase activity than lighter sage and black locust honey. Black locust honey has a naturally low enzyme activity and showed the highest acid phosphatase activity among the analysed honey types, while honeydew honey, otherwise known to possess high proline content and enzyme activity, had a low protein content comparable to black locust honey. Statistically significant correlations were obtained between all analysed parameters, with the exception of acid phosphatase activity.

ACS Style

Ivana Flanjak; Ivica Strelec; Daniela Čačić Kenjerić; Ljiljana Primorac. Croatian produced unifloral honey characterized according to the protein and proline content and enzyme activities. Journal of Apicultural Science 2016, 60, 39 -48.

AMA Style

Ivana Flanjak, Ivica Strelec, Daniela Čačić Kenjerić, Ljiljana Primorac. Croatian produced unifloral honey characterized according to the protein and proline content and enzyme activities. Journal of Apicultural Science. 2016; 60 (1):39-48.

Chicago/Turabian Style

Ivana Flanjak; Ivica Strelec; Daniela Čačić Kenjerić; Ljiljana Primorac. 2016. "Croatian produced unifloral honey characterized according to the protein and proline content and enzyme activities." Journal of Apicultural Science 60, no. 1: 39-48.

Journal article
Published: 19 December 2015 in Acta Veterinaria Brno
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The research focused on the effects of dietary replacement of 3% sunflower oil (SO group) with 3% milk thistle oil (MTO group) on the technological quality of meat, such as pH value, colour (CIE L*, CIE a*, CIE b*), drip loss (%), shear force (N), and cooking loss (%), as well as on the content of fatty acid lipids in broiler breast and thigh muscles. Significant difference (P < 0.05) was determined for pHi, pHu, CIE a*, CIE b* values between groups, although the values for the stated indicators were within the standard range. Lipids of breast meat of the MTO group contained more arachidic acid (P < 0.001), octadecenoic acid isomer B (P = 0.047) and eicosatrienoic acid (P = 0.041), and less α-linolenic acid (P < 0.001) and Σn-3PUFA. Lipids of thigh meat of the MTO group contained more ΣSFA, myristoleic acid, eicosatrienoic acid (P < 0.05) and eicosenoic acid (P < 0.001), and less α-linolenic acid, and had narrower Σn-3/n-6 PUFA ratio than the SO group. According to the antioxidant status of broiler liver, there was significantly higher catalase activity determined in the MTO group.

ACS Style

Gordana Kralik; Zlata Kralik; Eva Straková; Marcela Šperanda; Igor Kralik; Ivica Strelec. Influence of dietary replacement of sunflower oil with milk thistle (Silybum marianum) oil on chicken meat quality and antioxidant status of liver. Acta Veterinaria Brno 2015, 84, 373 -382.

AMA Style

Gordana Kralik, Zlata Kralik, Eva Straková, Marcela Šperanda, Igor Kralik, Ivica Strelec. Influence of dietary replacement of sunflower oil with milk thistle (Silybum marianum) oil on chicken meat quality and antioxidant status of liver. Acta Veterinaria Brno. 2015; 84 (4):373-382.

Chicago/Turabian Style

Gordana Kralik; Zlata Kralik; Eva Straková; Marcela Šperanda; Igor Kralik; Ivica Strelec. 2015. "Influence of dietary replacement of sunflower oil with milk thistle (Silybum marianum) oil on chicken meat quality and antioxidant status of liver." Acta Veterinaria Brno 84, no. 4: 373-382.

Journal article
Published: 16 August 2015 in Journal of Food Biochemistry
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The influence of extraction temperature (90–180C), solvent concentration (36–90% ethanol) and time (30–90 min) on extractability of phenolic compounds from barley grains and on antiradical power of extracts was examined. RSM was used to design and optimize the processing parameters. Experimental results of total phenolic contents, total flavonoids and antiradical power of barley extracts were in the range from 3.27 to 26.60 mgGAE/gdb, 0.91 to 6.09 mgCE/gdb and 0.33 to 4.05 gDPPH/mLext, respectively. HPLC analysis of extracts showed epigallocatechin gallate to be the predominant phenolic compound, representing up to 93% of all phenolic compounds determined. Based on RSM analysis, temperature was the most significant factor affecting the observed responses (P < 0.05) and optimal extraction conditions were achieved at 180C, 36% ethanol and 90 min. Additionally, strong correlation between protein content and phenolic compounds of extracts was observed (R = 0.925), indicating the influence of total proteins on possible overestimation of polyphenol content.Practical ApplicationsBarley is a cereal crop with potential health benefits related to its content of phenolic compounds. Extraction is one of the essential steps in the isolation of bioactive compounds from natural sources and it is well known that it depends on numerous parameters. The search for nonconventional method of extraction that can overcome drawbacks of conventional (solid‐liquid) extraction was the motivation of this research. Thus, the current study employed high‐pressure/high‐temperature stirred reactor for extraction process, and RSM for modeling the possible relationship between different extraction parameters and yield of phenolic compounds extracted from barley as well as their antioxidant activities. The data shown would provide the scientific rationale for better understanding of extraction of bioactive phenolic compounds from barley at high‐pressure/high‐temperature regime and as a contribution to the development of this technique in spite of the high cost of equipment.

ACS Style

Ana Bucić-Kojić; Alessandro A. Casazza; Ivica Strelec; Marco Paini; Mirela Planinić; Patrizia Perego. Influence of High-Pressure/High-Temperature Extraction on the Recovery of Phenolic Compounds from Barley Grains. Journal of Food Biochemistry 2015, 39, 696 -707.

AMA Style

Ana Bucić-Kojić, Alessandro A. Casazza, Ivica Strelec, Marco Paini, Mirela Planinić, Patrizia Perego. Influence of High-Pressure/High-Temperature Extraction on the Recovery of Phenolic Compounds from Barley Grains. Journal of Food Biochemistry. 2015; 39 (6):696-707.

Chicago/Turabian Style

Ana Bucić-Kojić; Alessandro A. Casazza; Ivica Strelec; Marco Paini; Mirela Planinić; Patrizia Perego. 2015. "Influence of High-Pressure/High-Temperature Extraction on the Recovery of Phenolic Compounds from Barley Grains." Journal of Food Biochemistry 39, no. 6: 696-707.

Journal article
Published: 15 July 2013 in Journal of Food Biochemistry
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I. Strelec; Bojan Šarkanj; V. Mrša; Ž. Ugarčić-Hardi. Chemical Composition, Quality Parameters, Exopeptidase and Oxidoreductase Activity Changes During Temporal Development of Wheat Grain Infestation by Sitophilus granarius. Journal of Food Biochemistry 2013, 38, 175 -183.

AMA Style

I. Strelec, Bojan Šarkanj, V. Mrša, Ž. Ugarčić-Hardi. Chemical Composition, Quality Parameters, Exopeptidase and Oxidoreductase Activity Changes During Temporal Development of Wheat Grain Infestation by Sitophilus granarius. Journal of Food Biochemistry. 2013; 38 (2):175-183.

Chicago/Turabian Style

I. Strelec; Bojan Šarkanj; V. Mrša; Ž. Ugarčić-Hardi. 2013. "Chemical Composition, Quality Parameters, Exopeptidase and Oxidoreductase Activity Changes During Temporal Development of Wheat Grain Infestation by Sitophilus granarius." Journal of Food Biochemistry 38, no. 2: 175-183.

Journal article
Published: 01 December 2012 in Food and Chemical Toxicology
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This study determined exposure of pregnant women to ochratoxin A (OTA). Forty samples of first-void urine samples from Croatian women in the third trimester of pregnancy were analyzed for OTA and its major metabolite ochratoxin alpha (OTα). The subjects filled a short food frequency questionnaire (FFQ). Analysis was performed by HPLC-FLD following liquid-liquid extraction. All samples were subjected in parallel to enzymatic treatment (β-glucuronidase/aryl sulfatase) to release OTA and OTα from the conjugates. The median urinary levels of OTA and OTα before treatment were 0.02 (range: nd-1.07) ng/mL and 0.16 (nd-1.86) ng/mL; the concentrations after enzyme hydrolysis were 0.02 (nd-1.11) ng/mL and 1.18 (0.11-7.57) ng/mL. While OTα levels increased significantly following enzymatic treatment, evidence for OTA conjugation was weak. The ratio of urinary OTα medians after and before hydrolysis was 1.5 times higher than previously reported for nonpregnant female subjects, possibly indicating upregulated metabolism and/or elimination of the mycotoxin and metabolites in pregnancy. The mean daily dietary OTA intake calculated from FFQs (1.08±0.57 ng/kg body weight) was well below the provisional tolerable daily intake and the greatest contributors to intake were cereal products, fruit juices, chocolate and coffee.

ACS Style

T. Klapec; B. Šarkanj; I. Banjari; I. Strelec. Urinary ochratoxin A and ochratoxin alpha in pregnant women. Food and Chemical Toxicology 2012, 50, 4487 -4492.

AMA Style

T. Klapec, B. Šarkanj, I. Banjari, I. Strelec. Urinary ochratoxin A and ochratoxin alpha in pregnant women. Food and Chemical Toxicology. 2012; 50 (12):4487-4492.

Chicago/Turabian Style

T. Klapec; B. Šarkanj; I. Banjari; I. Strelec. 2012. "Urinary ochratoxin A and ochratoxin alpha in pregnant women." Food and Chemical Toxicology 50, no. 12: 4487-4492.

Journal article
Published: 01 July 2012 in Journal of Stored Products Research
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ACS Style

Ivica Strelec; Lucija Kučko; Dragica Roknić; Vladimir Mrsa; Žaneta Ugarčić-Hardi. Spectrofluorimetric, spectrophotometric and chemometric analysis of wheat grains infested by Sitophilus granarius. Journal of Stored Products Research 2012, 50, 42 -48.

AMA Style

Ivica Strelec, Lucija Kučko, Dragica Roknić, Vladimir Mrsa, Žaneta Ugarčić-Hardi. Spectrofluorimetric, spectrophotometric and chemometric analysis of wheat grains infested by Sitophilus granarius. Journal of Stored Products Research. 2012; 50 ():42-48.

Chicago/Turabian Style

Ivica Strelec; Lucija Kučko; Dragica Roknić; Vladimir Mrsa; Žaneta Ugarčić-Hardi. 2012. "Spectrofluorimetric, spectrophotometric and chemometric analysis of wheat grains infested by Sitophilus granarius." Journal of Stored Products Research 50, no. : 42-48.